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The map label for this gene is dacF [H]

Identifier: 170755178

GI number: 170755178

Start: 1570256

End: 1571530

Strand: Reverse

Name: dacF [H]

Synonym: CLD_3133

Alternate gene names: 170755178

Gene position: 1571530-1570256 (Counterclockwise)

Preceding gene: 170755054

Following gene: 170755521

Centisome position: 39.7

GC content: 27.61

Gene sequence:

>1275_bases
ATGAGAAAAGGAAAAATGACTTTAATTTCACTATTAATTTTTATTCTCACCTTAACATCTAATGTATTTGCTGCAGGAAA
TTCAAATACTGAGTCCCCTACTATTTATGGTAAGACAGCTATGACTATAGATGTTGAAACTGGTGAAATAATATATGCTA
AAAATATTGACAAGCGTATGTATCCTGCTAGTACTACAAAATATTTAACAGCCCTTTTATTGGCTGAAAACAAAGGGAAA
AATGATAATATACCTTATACAGCTACTGCTAAAAGTCAACCACAATATTCTCTAAATATAAATCTTCATCCTATAGATAT
AGGAGAAACTATAAAAGCAGAAAATGTTATGGACGGATTATTACTTTTTTCTGGTAATGATGTAGCTTATATGATAGGTG
ATGCCTTAGCTGGTAATAATAAAAATTTTGAAAATATGATGAATAAAAAGATACAAGAGCTAAATTTAAAGAACACTCAC
TTTGTAACCCCTAATGGATTACATAATCCTAATCATTATACTACAGCTTATGATTTAAGTGTAATTAGTAGGGCTGCTCT
AAAAAATACTTGGGTTTACCAATCTTCTAATAAAAAAGAAAGCTCTATAGTCACTTCTAAAGGTACAAAAATGACTATAG
AAAATAGTAATAAGCTTTTAGGGAAAGATGGTTGCGTTGCAGGTAAAACAGGTTACACTGATGCTGCTGGTAGATGTTTA
GTAACTTTGTTTAATAGAGATGGTAGAAAAATACTTGGGGTAGTTATGGGATCTGTATATGATGCTAAAGATTCTTTCGT
TTTTCAAGATATGAAAAAAATTATAGATTGGAGTTATAATGTAAAACCATCCACTCTATATAAAAAAGACTCTAGCCTAA
AAACAGAAACAGTAACCTATAATCCTTTAAAAATTATAGGACCTTCTGTGACTATAGATGTGCCTATAATTTTAAAAGAA
GATATTACTTACTATAAAAATGAAGTAAATGATAAAGAAGCTAATAAAACAATCAATATAGATAATACTTCTTATGCTGC
TCTTTCTGGCAAAAAAAATATGGGAACTTTAACTGTAAAAGAAAGAGAATACGAAAAAAAATATGAAGTATACTCTGGTT
TACCTAAAGAAGAAATAATGAAAAAGAATATGCCTTTTTATGTGGTTTCTATAATTTTAGTACTAGCTATAATTACTGTA
ATATTCTTTTTAGTAAAATTTTTTAAAAATAAGATTAACAAAAAAAATAGAAATAAAAATAAATACTGGTATTAA

Upstream 100 bases:

>100_bases
AAAATATACTCTCTAATGCTATACTATCAAAGTGTATACATATAGTATATACTTAAAATTTTATATTCAAATTAGAAAAA
AGTAAATGGAGAGATGGCCT

Downstream 100 bases:

>100_bases
AATATCAATATTATTTACATCATAAAAAACTTGCTAATATACTTTAAATATATAATAAAAACACTATCAAAATATAAATG
ATAGTGTTTTTATTATATAC

Product: D-alanyl-D-alanine carboxypeptidase family protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MRKGKMTLISLLIFILTLTSNVFAAGNSNTESPTIYGKTAMTIDVETGEIIYAKNIDKRMYPASTTKYLTALLLAENKGK
NDNIPYTATAKSQPQYSLNINLHPIDIGETIKAENVMDGLLLFSGNDVAYMIGDALAGNNKNFENMMNKKIQELNLKNTH
FVTPNGLHNPNHYTTAYDLSVISRAALKNTWVYQSSNKKESSIVTSKGTKMTIENSNKLLGKDGCVAGKTGYTDAAGRCL
VTLFNRDGRKILGVVMGSVYDAKDSFVFQDMKKIIDWSYNVKPSTLYKKDSSLKTETVTYNPLKIIGPSVTIDVPIILKE
DITYYKNEVNDKEANKTINIDNTSYAALSGKKNMGTLTVKEREYEKKYEVYSGLPKEEIMKKNMPFYVVSIILVLAIITV
IFFLVKFFKNKINKKNRNKNKYWY

Sequences:

>Translated_424_residues
MRKGKMTLISLLIFILTLTSNVFAAGNSNTESPTIYGKTAMTIDVETGEIIYAKNIDKRMYPASTTKYLTALLLAENKGK
NDNIPYTATAKSQPQYSLNINLHPIDIGETIKAENVMDGLLLFSGNDVAYMIGDALAGNNKNFENMMNKKIQELNLKNTH
FVTPNGLHNPNHYTTAYDLSVISRAALKNTWVYQSSNKKESSIVTSKGTKMTIENSNKLLGKDGCVAGKTGYTDAAGRCL
VTLFNRDGRKILGVVMGSVYDAKDSFVFQDMKKIIDWSYNVKPSTLYKKDSSLKTETVTYNPLKIIGPSVTIDVPIILKE
DITYYKNEVNDKEANKTINIDNTSYAALSGKKNMGTLTVKEREYEKKYEVYSGLPKEEIMKKNMPFYVVSIILVLAIITV
IFFLVKFFKNKINKKNRNKNKYWY
>Mature_424_residues
MRKGKMTLISLLIFILTLTSNVFAAGNSNTESPTIYGKTAMTIDVETGEIIYAKNIDKRMYPASTTKYLTALLLAENKGK
NDNIPYTATAKSQPQYSLNINLHPIDIGETIKAENVMDGLLLFSGNDVAYMIGDALAGNNKNFENMMNKKIQELNLKNTH
FVTPNGLHNPNHYTTAYDLSVISRAALKNTWVYQSSNKKESSIVTSKGTKMTIENSNKLLGKDGCVAGKTGYTDAAGRCL
VTLFNRDGRKILGVVMGSVYDAKDSFVFQDMKKIIDWSYNVKPSTLYKKDSSLKTETVTYNPLKIIGPSVTIDVPIILKE
DITYYKNEVNDKEANKTINIDNTSYAALSGKKNMGTLTVKEREYEKKYEVYSGLPKEEIMKKNMPFYVVSIILVLAIITV
IFFLVKFFKNKINKKNRNKNKYWY

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=312, Percent_Identity=28.525641025641, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787062, Length=305, Percent_Identity=26.2295081967213, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786851, Length=284, Percent_Identity=28.8732394366197, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082054, Length=254, Percent_Identity=27.9527559055118, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47686; Mature: 47686

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKGKMTLISLLIFILTLTSNVFAAGNSNTESPTIYGKTAMTIDVETGEIIYAKNIDKRM
CCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEECEEEEEEECCCCEEEEECCCCCC
YPASTTKYLTALLLAENKGKNDNIPYTATAKSQPQYSLNINLHPIDIGETIKAENVMDGL
CCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCCCCCHHHHHCEE
LLFSGNDVAYMIGDALAGNNKNFENMMNKKIQELNLKNTHFVTPNGLHNPNHYTTAYDLS
EEEECCCEEEEEECHHCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEHHH
VISRAALKNTWVYQSSNKKESSIVTSKGTKMTIENSNKLLGKDGCVAGKTGYTDAAGRCL
HHHHHHHHCCEEEECCCCCCCCEEECCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCEE
VTLFNRDGRKILGVVMGSVYDAKDSFVFQDMKKIIDWSYNVKPSTLYKKDSSLKTETVTY
EEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEEEE
NPLKIIGPSVTIDVPIILKEDITYYKNEVNDKEANKTINIDNTSYAALSGKKNMGTLTVK
CCEEEECCCEEEEEEEEEECCCHHHHHCCCCCCCCCEEEECCCCEEEECCCCCCCEEEEE
EREYEKKYEVYSGLPKEEIMKKNMPFYVVSIILVLAIITVIFFLVKFFKNKINKKNRNKN
HHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KYWY
CCCC
>Mature Secondary Structure
MRKGKMTLISLLIFILTLTSNVFAAGNSNTESPTIYGKTAMTIDVETGEIIYAKNIDKRM
CCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEECEEEEEEECCCCEEEEECCCCCC
YPASTTKYLTALLLAENKGKNDNIPYTATAKSQPQYSLNINLHPIDIGETIKAENVMDGL
CCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCCCCCHHHHHCEE
LLFSGNDVAYMIGDALAGNNKNFENMMNKKIQELNLKNTHFVTPNGLHNPNHYTTAYDLS
EEEECCCEEEEEECHHCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEHHH
VISRAALKNTWVYQSSNKKESSIVTSKGTKMTIENSNKLLGKDGCVAGKTGYTDAAGRCL
HHHHHHHHCCEEEECCCCCCCCEEECCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCEE
VTLFNRDGRKILGVVMGSVYDAKDSFVFQDMKKIIDWSYNVKPSTLYKKDSSLKTETVTY
EEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEEEE
NPLKIIGPSVTIDVPIILKEDITYYKNEVNDKEANKTINIDNTSYAALSGKKNMGTLTVK
CCEEEECCCEEEEEEEEEECCCHHHHHCCCCCCCCCEEEECCCCEEEECCCCCCCEEEEE
EREYEKKYEVYSGLPKEEIMKKNMPFYVVSIILVLAIITVIFFLVKFFKNKINKKNRNKN
HHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KYWY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]