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The map label for this gene is aspC

Identifier: 170754606

GI number: 170754606

Start: 1131065

End: 1132354

Strand: Reverse

Name: aspC

Synonym: CLD_3547

Alternate gene names: 170754606

Gene position: 1132354-1131065 (Counterclockwise)

Preceding gene: 170755346

Following gene: 170754809

Centisome position: 28.61

GC content: 28.68

Gene sequence:

>1290_bases
ATGAAAAGAACATTAATAAGTGAATTGAAAAATTTTATAAATGAAAATGTAAATCTTCAAGGATGGATTCATAAAATTAG
GAAATTGAAGAATATAACATTTTTAATAATAAGAGATAGATCAGGATTGATTCAATGTATTGTAGATAATGATAAATTGG
ATTTATCGCAGGTAAAGATAGAATCTATAGTATCAATGACAGGAGTTGTTAAAGAAAGTACTAATGCTTTAAATCCTTTT
GAAATACAAGTAGAAAATTTAGAAATTATAAATGCTGCTTTAGATAAAGTTCCAATAGAAATAAATAAAGAAAATCTAGA
GATTAATTTAGATACTATGCTTAACAATAGGATATTAAGCTTAAGGCACCCTAAGGTAAATTCAATATTTAAAGTTCAAA
ACTCAATAGTAAATGGATTTAGAGAATTTTTAAATAAAGAAGAATTTACTGAAATTTATACTCCAAAGATTGTATGGGAA
GGAGCTGAAGGTGGTACAGAAGTATTTAAGGTAAAATACTTTGAAAATACTGCTTATTTAGCCCAAAGCCCTCAATTCTA
CAAACAGATGATGGTAGGAGCTGGATTTGAAAAGGTATTTGAAATAGGTCATGCCTATAGGGCAGAAGAGCATAATACTA
ATAGACATTTAAATGAATATATTAGTATGGACTTGGAAATTGGATTTATAGAAGATGAAAAAGATATTATGGAAGTTGAG
GAAAGGTTATTAAAATTCATATTAGAAAAACTTAATAAAGAATGTAAGGAATATTTTGACCTACTGGATTCAGAATTACC
AAAAATAGAATCCGAAATACCTAAGATTACATTTAAGGAGGCATTAGATATATTAAGTAAGGAATATAATAAAAATAACT
TAACTTGTGACTTAGATCCAGAAGGAGAAAAACTCATATGTAAGTATGCAAAGGAAAAGCTAAATAGTGATTTCATATTT
TTAACTAACTATCCTAGAGAAAAGAGACCAATGTATACCATGCCTCTAGGTAAAGATGGAACTCATAGTTTCGACCTGTT
ATTTAGAGGTATAGAGATTACTACAGGTGGGCAAAGAATTCACGACTATAATATGCTAGTAGATAATATAGATTTTAAAG
GATTTAATCCTAAGGACTATGAGAGTTATTTATCTGCTTTTAAGTATGGAATGCCTAAGCATGGAGGCCTTGCCATTGGA
TTAGAACGACTAACATCAAGACTTCTAGGATTAGAAAATGTTAGAGAAGCTGCATTAATTACAAGAGATAGAACTCGTTT
ACTTCCTTAA

Upstream 100 bases:

>100_bases
CTAATTTTGTATAATCATTGTTAAAAAGGGTGGTACCGCAGATTATTTTGCCCCTTGGGTTTTTACTCAAGGGGCTTATT
TTATTTAGGAGGTAATAAAT

Downstream 100 bases:

>100_bases
AGTTACTCTATTATCACAATTGTCTTCCTAGCTCTTTATAAATTTCAATATCTTATTTATTCATAAGTATCTTAGGGAGG
AACATTAATAATTTACTCCC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKRTLISELKNFINENVNLQGWIHKIRKLKNITFLIIRDRSGLIQCIVDNDKLDLSQVKIESIVSMTGVVKESTNALNPF
EIQVENLEIINAALDKVPIEINKENLEINLDTMLNNRILSLRHPKVNSIFKVQNSIVNGFREFLNKEEFTEIYTPKIVWE
GAEGGTEVFKVKYFENTAYLAQSPQFYKQMMVGAGFEKVFEIGHAYRAEEHNTNRHLNEYISMDLEIGFIEDEKDIMEVE
ERLLKFILEKLNKECKEYFDLLDSELPKIESEIPKITFKEALDILSKEYNKNNLTCDLDPEGEKLICKYAKEKLNSDFIF
LTNYPREKRPMYTMPLGKDGTHSFDLLFRGIEITTGGQRIHDYNMLVDNIDFKGFNPKDYESYLSAFKYGMPKHGGLAIG
LERLTSRLLGLENVREAALITRDRTRLLP

Sequences:

>Translated_429_residues
MKRTLISELKNFINENVNLQGWIHKIRKLKNITFLIIRDRSGLIQCIVDNDKLDLSQVKIESIVSMTGVVKESTNALNPF
EIQVENLEIINAALDKVPIEINKENLEINLDTMLNNRILSLRHPKVNSIFKVQNSIVNGFREFLNKEEFTEIYTPKIVWE
GAEGGTEVFKVKYFENTAYLAQSPQFYKQMMVGAGFEKVFEIGHAYRAEEHNTNRHLNEYISMDLEIGFIEDEKDIMEVE
ERLLKFILEKLNKECKEYFDLLDSELPKIESEIPKITFKEALDILSKEYNKNNLTCDLDPEGEKLICKYAKEKLNSDFIF
LTNYPREKRPMYTMPLGKDGTHSFDLLFRGIEITTGGQRIHDYNMLVDNIDFKGFNPKDYESYLSAFKYGMPKHGGLAIG
LERLTSRLLGLENVREAALITRDRTRLLP
>Mature_429_residues
MKRTLISELKNFINENVNLQGWIHKIRKLKNITFLIIRDRSGLIQCIVDNDKLDLSQVKIESIVSMTGVVKESTNALNPF
EIQVENLEIINAALDKVPIEINKENLEINLDTMLNNRILSLRHPKVNSIFKVQNSIVNGFREFLNKEEFTEIYTPKIVWE
GAEGGTEVFKVKYFENTAYLAQSPQFYKQMMVGAGFEKVFEIGHAYRAEEHNTNRHLNEYISMDLEIGFIEDEKDIMEVE
ERLLKFILEKLNKECKEYFDLLDSELPKIESEIPKITFKEALDILSKEYNKNNLTCDLDPEGEKLICKYAKEKLNSDFIF
LTNYPREKRPMYTMPLGKDGTHSFDLLFRGIEITTGGQRIHDYNMLVDNIDFKGFNPKDYESYLSAFKYGMPKHGGLAIG
LERLTSRLLGLENVREAALITRDRTRLLP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI45439306, Length=445, Percent_Identity=34.3820224719101, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI4758762, Length=438, Percent_Identity=26.7123287671233, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI226491502, Length=445, Percent_Identity=26.5168539325843, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI40789249, Length=252, Percent_Identity=30.5555555555556, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI1787161, Length=452, Percent_Identity=26.1061946902655, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788173, Length=265, Percent_Identity=27.9245283018868, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1789256, Length=451, Percent_Identity=24.1685144124169, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790571, Length=451, Percent_Identity=22.8381374722838, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17551876, Length=451, Percent_Identity=34.589800443459, Blast_Score=262, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI71984122, Length=448, Percent_Identity=28.7946428571429, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17556372, Length=415, Percent_Identity=25.7831325301205, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI32566633, Length=283, Percent_Identity=26.8551236749117, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323011, Length=452, Percent_Identity=34.5132743362832, Blast_Score=266, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6321807, Length=448, Percent_Identity=28.5714285714286, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6319872, Length=476, Percent_Identity=23.9495798319328, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6325153, Length=264, Percent_Identity=28.4090909090909, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI17136276, Length=458, Percent_Identity=33.1877729257642, Blast_Score=261, Evalue=8e-70,
Organism=Drosophila melanogaster, GI19921528, Length=440, Percent_Identity=30, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI19922416, Length=460, Percent_Identity=23.2608695652174, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24584738, Length=288, Percent_Identity=27.7777777777778, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24640851, Length=468, Percent_Identity=21.5811965811966, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24640849, Length=468, Percent_Identity=21.5811965811966, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020780
- InterPro:   IPR004523
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 49817; Mature: 49817

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTLISELKNFINENVNLQGWIHKIRKLKNITFLIIRDRSGLIQCIVDNDKLDLSQVKI
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCCHHHHHH
ESIVSMTGVVKESTNALNPFEIQVENLEIINAALDKVPIEINKENLEINLDTMLNNRILS
HHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCEEEECCCEEEEEEEHHCCCEEE
LRHPKVNSIFKVQNSIVNGFREFLNKEEFTEIYTPKIVWEGAEGGTEVFKVKYFENTAYL
ECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCEEEEECCCCCCEEEEEEEECCCCHH
AQSPQFYKQMMVGAGFEKVFEIGHAYRAEEHNTNRHLNEYISMDLEIGFIEDEKDIMEVE
HCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEEEEECCHHHHHHHH
ERLLKFILEKLNKECKEYFDLLDSELPKIESEIPKITFKEALDILSKEYNKNNLTCDLDP
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCEEHHHHHHHHHHHCCCCCEEEEECC
EGEKLICKYAKEKLNSDFIFLTNYPREKRPMYTMPLGKDGTHSFDLLFRGIEITTGGQRI
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCCCCCCHHHEEECEEEECCCCEE
HDYNMLVDNIDFKGFNPKDYESYLSAFKYGMPKHGGLAIGLERLTSRLLGLENVREAALI
EEHHHEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHCHHHHHHHHHH
TRDRTRLLP
CCCHHHCCC
>Mature Secondary Structure
MKRTLISELKNFINENVNLQGWIHKIRKLKNITFLIIRDRSGLIQCIVDNDKLDLSQVKI
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCCHHHHHH
ESIVSMTGVVKESTNALNPFEIQVENLEIINAALDKVPIEINKENLEINLDTMLNNRILS
HHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCEEEECCCEEEEEEEHHCCCEEE
LRHPKVNSIFKVQNSIVNGFREFLNKEEFTEIYTPKIVWEGAEGGTEVFKVKYFENTAYL
ECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCEEEEECCCCCCEEEEEEEECCCCHH
AQSPQFYKQMMVGAGFEKVFEIGHAYRAEEHNTNRHLNEYISMDLEIGFIEDEKDIMEVE
HCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEEEEECCHHHHHHHH
ERLLKFILEKLNKECKEYFDLLDSELPKIESEIPKITFKEALDILSKEYNKNNLTCDLDP
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCEEHHHHHHHHHHHCCCCCEEEEECC
EGEKLICKYAKEKLNSDFIFLTNYPREKRPMYTMPLGKDGTHSFDLLFRGIEITTGGQRI
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCCCCCCHHHEEECEEEECCCCEE
HDYNMLVDNIDFKGFNPKDYESYLSAFKYGMPKHGGLAIGLERLTSRLLGLENVREAALI
EEHHHEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHCHHHHHHHHHH
TRDRTRLLP
CCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]