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The map label for this gene is purB [H]

Identifier: 170752003

GI number: 170752003

Start: 5964505

End: 5965812

Strand: Direct

Name: purB [H]

Synonym: Mrad2831_5635

Alternate gene names: 170752003

Gene position: 5964505-5965812 (Clockwise)

Preceding gene: 170752002

Following gene: 170752004

Centisome position: 98.14

GC content: 69.04

Gene sequence:

>1308_bases
ATGATCCCCCGCTACAGCCGCCCCGCGATGACGGCGATCTGGTCGCCCGAGAGCCGTTTCCGGATCTGGTTCGAGATCGA
GGCCCACGCCACCACGGCGCTCGCCGAGATCGGCGTCGTTCCGAAGGAGGCGGCCGAGACGATCTGGAGCAAGGGCAAGG
ACGCGGCCTTCGACGTCGCTCGCATCGACGCGATCGAGGCGGTGACGAAGCACGACGTCATCGCCTTCCTGACCCACCTC
GCCGAGATCGTCGGGCCCGAGGCGCGATTCGTCCACCAGGGCATGACCTCCTCGGACGTCCTCGACACCTGCCTCAACGT
GCAGCTGGTACGCGCCGCCGACATCCTGATCGCCGACGTCGACTCGCTGCTCGCCGCCCTGAAGGCGCGGGCCTTCGAGC
ACAAGATGACCCCGACCATCGGCCGGTCGCACGGTATCCACGCCGAGCCGGTGACCTTCGGTCTGAAGCTCGCCCAGGCC
TATGCCGAGTTCGAGCGCAACCGCCGCCGCCTCGTGGCGGCCCGCGAGGAGGTCGCCACCTGCGCGATCTCCGGCGCGGT
CGGCACGTTCGCCAATATCGACCCGCGGGTGGAGCAGTACGTCGCCGAGAAGATGGGTCTGACCGCCGAGCCGGTCTCGA
CCCAGGTGATCCCGCGCGACCGGCACGCGATGTTCTTCGCGACCCTCGGCGTCGTCGCCTCGTCGCTGGAGCGCTTGGCG
ATCGAGATCCGCCACCTGCAGCGCACCGAGGTCCTGGAGGCGGAGGAGTTCTTCTCCGAGGGGCAGAAGGGTTCCTCGGC
CATGCCGCACAAGCGCAACCCGGTGCTCACCGAGAACATCACGGGGCTCGCCCGGATGGTACGCGGCTACGTCACGCCGG
CCCTGGAGAACGTCGCCCTCTGGCACGAGCGCGACATCTCCCACTCGTCGGTGGAGCGGATGATCGGCCCCGACGCCACC
GTCACCCTCGACTTCGCCCTGGCGCGGATGACCGGGGTGATGGAGAAGCTGCTGGTCTACCCGGCCAACATGCAGAAGAA
CCTCGACCGGCTCGGCGGCCTGATCCACTCGCAGCGCGTGCTGCTGGCGCTGACCCAGGCCGGGGTCTCCCGCGAGGATT
CCTACCGGCTGGTCCAGCGCAACGCGATGCCGGTCTGGCGCGGCGAGGGCGACTTCCTGGCGCTGCTCAAGGCGGACCCG
GAGGTCGCCGCGGCACTCCCGGCCGAGCAGATCGAGGCGTGCTTCGATCTCGGCTACCACTTCAAGCACGTGGACACGAT
CTTCGGGCGGGTCTTCGGCGACCGCTGA

Upstream 100 bases:

>100_bases
CCACATCCGTTGCCGCCTTGACATGGCCGATGCGCATTGAGCGGCCGGGCCGGCGCATGCTACCGGCCTCGCACGCAATC
CGGACCGTGCCGAGGCCCCC

Downstream 100 bases:

>100_bases
GGGTCCCGCACGATTCCTGCTTCGACCCGCCCTCGCGGGCCCGCGTTCCCGGGCCCGCGACGAACACAGGACAGCTCCCC
GTGACCGCTCCCCGCATCGT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MIPRYSRPAMTAIWSPESRFRIWFEIEAHATTALAEIGVVPKEAAETIWSKGKDAAFDVARIDAIEAVTKHDVIAFLTHL
AEIVGPEARFVHQGMTSSDVLDTCLNVQLVRAADILIADVDSLLAALKARAFEHKMTPTIGRSHGIHAEPVTFGLKLAQA
YAEFERNRRRLVAAREEVATCAISGAVGTFANIDPRVEQYVAEKMGLTAEPVSTQVIPRDRHAMFFATLGVVASSLERLA
IEIRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLTENITGLARMVRGYVTPALENVALWHERDISHSSVERMIGPDAT
VTLDFALARMTGVMEKLLVYPANMQKNLDRLGGLIHSQRVLLALTQAGVSREDSYRLVQRNAMPVWRGEGDFLALLKADP
EVAAALPAEQIEACFDLGYHFKHVDTIFGRVFGDR

Sequences:

>Translated_435_residues
MIPRYSRPAMTAIWSPESRFRIWFEIEAHATTALAEIGVVPKEAAETIWSKGKDAAFDVARIDAIEAVTKHDVIAFLTHL
AEIVGPEARFVHQGMTSSDVLDTCLNVQLVRAADILIADVDSLLAALKARAFEHKMTPTIGRSHGIHAEPVTFGLKLAQA
YAEFERNRRRLVAAREEVATCAISGAVGTFANIDPRVEQYVAEKMGLTAEPVSTQVIPRDRHAMFFATLGVVASSLERLA
IEIRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLTENITGLARMVRGYVTPALENVALWHERDISHSSVERMIGPDAT
VTLDFALARMTGVMEKLLVYPANMQKNLDRLGGLIHSQRVLLALTQAGVSREDSYRLVQRNAMPVWRGEGDFLALLKADP
EVAAALPAEQIEACFDLGYHFKHVDTIFGRVFGDR
>Mature_435_residues
MIPRYSRPAMTAIWSPESRFRIWFEIEAHATTALAEIGVVPKEAAETIWSKGKDAAFDVARIDAIEAVTKHDVIAFLTHL
AEIVGPEARFVHQGMTSSDVLDTCLNVQLVRAADILIADVDSLLAALKARAFEHKMTPTIGRSHGIHAEPVTFGLKLAQA
YAEFERNRRRLVAAREEVATCAISGAVGTFANIDPRVEQYVAEKMGLTAEPVSTQVIPRDRHAMFFATLGVVASSLERLA
IEIRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLTENITGLARMVRGYVTPALENVALWHERDISHSSVERMIGPDAT
VTLDFALARMTGVMEKLLVYPANMQKNLDRLGGLIHSQRVLLALTQAGVSREDSYRLVQRNAMPVWRGEGDFLALLKADP
EVAAALPAEQIEACFDLGYHFKHVDTIFGRVFGDR

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=403, Percent_Identity=26.7990074441687, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI183227688, Length=397, Percent_Identity=26.1964735516373, Blast_Score=127, Evalue=3e-29,
Organism=Escherichia coli, GI1787376, Length=382, Percent_Identity=29.3193717277487, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17508577, Length=396, Percent_Identity=25.2525252525253, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI32564234, Length=362, Percent_Identity=25.9668508287293, Blast_Score=85, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323391, Length=416, Percent_Identity=27.4038461538462, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24647570, Length=436, Percent_Identity=25.2293577981651, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48129; Mature: 48129

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSRPAMTAIWSPESRFRIWFEIEAHATTALAEIGVVPKEAAETIWSKGKDAAFDVA
CCCCCCCCCEEEEECCCCCEEEEEEEECCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHH
RIDAIEAVTKHDVIAFLTHLAEIVGPEARFVHQGMTSSDVLDTCLNVQLVRAADILIADV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DSLLAALKARAFEHKMTPTIGRSHGIHAEPVTFGLKLAQAYAEFERNRRRLVAAREEVAT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFANIDPRVEQYVAEKMGLTAEPVSTQVIPRDRHAMFFATLGVVASSLERLA
HHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IEIRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLTENITGLARMVRGYVTPALENVAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERMIGPDATVTLDFALARMTGVMEKLLVYPANMQKNLDRLGGLIHSQRV
HHHCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LLALTQAGVSREDSYRLVQRNAMPVWRGEGDFLALLKADPEVAAALPAEQIEACFDLGYH
HHHHHHHCCCCCCCHHHHHHCCCCEEECCCCEEEEEECCCHHHHHCCHHHHHHHHHHCCH
FKHVDTIFGRVFGDR
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPRYSRPAMTAIWSPESRFRIWFEIEAHATTALAEIGVVPKEAAETIWSKGKDAAFDVA
CCCCCCCCCEEEEECCCCCEEEEEEEECCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHH
RIDAIEAVTKHDVIAFLTHLAEIVGPEARFVHQGMTSSDVLDTCLNVQLVRAADILIADV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DSLLAALKARAFEHKMTPTIGRSHGIHAEPVTFGLKLAQAYAEFERNRRRLVAAREEVAT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFANIDPRVEQYVAEKMGLTAEPVSTQVIPRDRHAMFFATLGVVASSLERLA
HHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IEIRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLTENITGLARMVRGYVTPALENVAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERMIGPDATVTLDFALARMTGVMEKLLVYPANMQKNLDRLGGLIHSQRV
HHHCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LLALTQAGVSREDSYRLVQRNAMPVWRGEGDFLALLKADPEVAAALPAEQIEACFDLGYH
HHHHHHHCCCCCCCHHHHHHCCCCEEECCCCEEEEEECCCHHHHHCCHHHHHHHHHHCCH
FKHVDTIFGRVFGDR
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]