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The map label for this gene is nadB [H]

Identifier: 170751832

GI number: 170751832

Start: 5775298

End: 5776821

Strand: Direct

Name: nadB [H]

Synonym: Mrad2831_5464

Alternate gene names: 170751832

Gene position: 5775298-5776821 (Clockwise)

Preceding gene: 170751831

Following gene: 170751833

Centisome position: 95.02

GC content: 77.82

Gene sequence:

>1524_bases
ATGAACGCCGTCTCTCCCAACAACGCCGACCGCGTGGTCGTGGTCGGCGGCGGGGTCGCGGGCCTCAGCGTCGCGCTGCG
CCTCGCGCCCCGCCCGGTCACGCTGGTCACCGCGTCGGCGCTCGGCCTCGGCACCGCCACCGGGTGGGCCCAGGGCGGCA
TCGCCGCCGCGGTCGGCGCCGACGACGCGGCGGCGCTCCACGCCGCCGACACGGAGGCCGCCGGCGCCGGGCTGACCGAA
CCCGACGTGGCGCTGCGGGTCGCCGGGGAGGGGCCCGCGCTGATCGACTGGCTGGTGCGGATCGGCGTCCCGTTCGACCG
CACCGACGACGCCGCGCTGGCCCTCGGGCTCGAGGCGGCGCACGGCCGCCGCCGCATCGTCCGCTCCGGCGGCGACGCCA
CCGGCGCCCGCGTCCTCGAAGGGCTGGTGCGGGCCGTGCTGGAGGCGCCCTCCGTCGAGATCGTCACCGCGCGAGCGTGC
GATCTCCTGCGGGACACGAATGGGGCGGTGGCGGGCATTGTCGCCGAGCGAGATGGCGCGCTGTTCCGCATCCCGGCCCG
CGCCGTGGTGCTCGCTACCGGCGGCGTCGGCGCGCTCTATGCCGCGACGACGAACCCGCCGGGCGCCGTCGGCCGCGGGC
TGGCGCTCGCCGCCCGGGCCGGCGCGGTGATGCGCGACATGGAGTTCGTCCAGTTCCACCCGACCGCCATCGCGGCCGGC
GCCGACCCGATGCCGCTCGCCACCGAGGCCCTGCGCGGTGAGGGTGCCCGCCTCATCGACGAGACCGGCGCCGCCGTGAT
GGCCGGCATCGCGGGCGGCGACCTCGCCCCCCGCGACGTGGTCGCCCGCGGCATCTTCCAGGCGCTGCAGCGGGGCCGCA
CGGTGTACCTCGACGCCCGGGGCGCACTCGGGGCCGCGATGCCGACGCGCTTCCCGACGGTGGCGGGGCTCTGCGCCGCC
GCCGGCATCGACCCGGCCGTGCAGGCGATTCCCGTCCGTCCGGCCGCGCACTACCACATGGGCGGCGTGAAGGTGGACGC
CCACGGCGCCACCTCGGTGCCGGGCCTCTACGCCTGCGGCGAGGTCTCCTCCACGGGCCTCCACGGCGCCAACCGGCTCG
CCAGCAACTCCCTGCTGGAGGGCTTGGCCTACGCGCGCTTCATCGCCGACGGTCTCGACGCGCCGCCGCCCGCCGCCATC
GTGCCGGAGGCCGTGTCCGCGCGCGCCGCCGGCGATCTGCCGGCGATCCGCGCGCTGATGGAGAGCAGAGTCGGCGTCGT
GCGCGACGCCGGGGGGCTCGCCGAGGCCGTCGCCGTCCTGGAGCCCGGGGCGGAGACCTGCGACGCCGCCCTCGTCGCAC
TGCTCATCGCCCGCGGCGCGCTCGCCCGCCGGGAGAGCCGCGGCGCCCACTGGCGCAGCGACTTCCCCCACCTCGCTCCG
GCCGCGCACACCGAAGCGACGCTGTTTGACGCGATCGCCGCGCCCCGCGCGACTCCGTCCACCGAGGAGGTGCTGACGCC
ATGA

Upstream 100 bases:

>100_bases
GGCGGTCGCTCGAGACGATGACCCACGAGGTCACGGTCGATCCGGCGCTGGCCGACCGCGCCCGGGCAGCGGTGGAGCGC
ATGCTCCAGGTGCGCACGCG

Downstream 100 bases:

>100_bases
TTCAGTCGGTTCTCTCCGAAGAGATCCTTCCCCTGCCGCGGCTCCTCGTGGAGCCCGTGGTGCGCGCCGCCCTGCTGGAG
GATCTCGGCCGGGCCGGCGA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MNAVSPNNADRVVVVGGGVAGLSVALRLAPRPVTLVTASALGLGTATGWAQGGIAAAVGADDAAALHAADTEAAGAGLTE
PDVALRVAGEGPALIDWLVRIGVPFDRTDDAALALGLEAAHGRRRIVRSGGDATGARVLEGLVRAVLEAPSVEIVTARAC
DLLRDTNGAVAGIVAERDGALFRIPARAVVLATGGVGALYAATTNPPGAVGRGLALAARAGAVMRDMEFVQFHPTAIAAG
ADPMPLATEALRGEGARLIDETGAAVMAGIAGGDLAPRDVVARGIFQALQRGRTVYLDARGALGAAMPTRFPTVAGLCAA
AGIDPAVQAIPVRPAAHYHMGGVKVDAHGATSVPGLYACGEVSSTGLHGANRLASNSLLEGLAYARFIADGLDAPPPAAI
VPEAVSARAAGDLPAIRALMESRVGVVRDAGGLAEAVAVLEPGAETCDAALVALLIARGALARRESRGAHWRSDFPHLAP
AAHTEATLFDAIAAPRATPSTEEVLTP

Sequences:

>Translated_507_residues
MNAVSPNNADRVVVVGGGVAGLSVALRLAPRPVTLVTASALGLGTATGWAQGGIAAAVGADDAAALHAADTEAAGAGLTE
PDVALRVAGEGPALIDWLVRIGVPFDRTDDAALALGLEAAHGRRRIVRSGGDATGARVLEGLVRAVLEAPSVEIVTARAC
DLLRDTNGAVAGIVAERDGALFRIPARAVVLATGGVGALYAATTNPPGAVGRGLALAARAGAVMRDMEFVQFHPTAIAAG
ADPMPLATEALRGEGARLIDETGAAVMAGIAGGDLAPRDVVARGIFQALQRGRTVYLDARGALGAAMPTRFPTVAGLCAA
AGIDPAVQAIPVRPAAHYHMGGVKVDAHGATSVPGLYACGEVSSTGLHGANRLASNSLLEGLAYARFIADGLDAPPPAAI
VPEAVSARAAGDLPAIRALMESRVGVVRDAGGLAEAVAVLEPGAETCDAALVALLIARGALARRESRGAHWRSDFPHLAP
AAHTEATLFDAIAAPRATPSTEEVLTP
>Mature_507_residues
MNAVSPNNADRVVVVGGGVAGLSVALRLAPRPVTLVTASALGLGTATGWAQGGIAAAVGADDAAALHAADTEAAGAGLTE
PDVALRVAGEGPALIDWLVRIGVPFDRTDDAALALGLEAAHGRRRIVRSGGDATGARVLEGLVRAVLEAPSVEIVTARAC
DLLRDTNGAVAGIVAERDGALFRIPARAVVLATGGVGALYAATTNPPGAVGRGLALAARAGAVMRDMEFVQFHPTAIAAG
ADPMPLATEALRGEGARLIDETGAAVMAGIAGGDLAPRDVVARGIFQALQRGRTVYLDARGALGAAMPTRFPTVAGLCAA
AGIDPAVQAIPVRPAAHYHMGGVKVDAHGATSVPGLYACGEVSSTGLHGANRLASNSLLEGLAYARFIADGLDAPPPAAI
VPEAVSARAAGDLPAIRALMESRVGVVRDAGGLAEAVAVLEPGAETCDAALVALLIARGALARRESRGAHWRSDFPHLAP
AAHTEATLFDAIAAPRATPSTEEVLTP

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=505, Percent_Identity=33.0693069306931, Blast_Score=185, Evalue=7e-47,
Organism=Escherichia coli, GI1788928, Length=524, Percent_Identity=36.6412213740458, Blast_Score=264, Evalue=8e-72,
Organism=Escherichia coli, GI1790597, Length=498, Percent_Identity=33.5341365461847, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1786942, Length=504, Percent_Identity=31.1507936507937, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17550100, Length=504, Percent_Identity=32.7380952380952, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17505833, Length=503, Percent_Identity=31.8091451292247, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI71986328, Length=319, Percent_Identity=30.4075235109718, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322701, Length=514, Percent_Identity=31.1284046692607, Blast_Score=187, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6322416, Length=504, Percent_Identity=31.3492063492063, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6320788, Length=474, Percent_Identity=26.7932489451477, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322511, Length=469, Percent_Identity=24.3070362473348, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI17137288, Length=505, Percent_Identity=32.0792079207921, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24655642, Length=505, Percent_Identity=32.0792079207921, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24655647, Length=505, Percent_Identity=32.0792079207921, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24663005, Length=509, Percent_Identity=28.4872298624754, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 50740; Mature: 50740

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00260 GLUCAGON

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVSPNNADRVVVVGGGVAGLSVALRLAPRPVTLVTASALGLGTATGWAQGGIAAAVGA
CCCCCCCCCCEEEEEECCHHHHHHEEEECCCCEEEEEEHHHCCCCCCCCCCCCEEEEECC
DDAAALHAADTEAAGAGLTEPDVALRVAGEGPALIDWLVRIGVPFDRTDDAALALGLEAA
CCHHEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEEHHHH
HGRRRIVRSGGDATGARVLEGLVRAVLEAPSVEIVTARACDLLRDTNGAVAGIVAERDGA
CCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCEEEEEEECCCC
LFRIPARAVVLATGGVGALYAATTNPPGAVGRGLALAARAGAVMRDMEFVQFHPTAIAAG
EEEECCEEEEEEECCCCEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHEECCCEEECC
ADPMPLATEALRGEGARLIDETGAAVMAGIAGGDLAPRDVVARGIFQALQRGRTVYLDAR
CCCCCHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECC
GALGAAMPTRFPTVAGLCAAAGIDPAVQAIPVRPAAHYHMGGVKVDAHGATSVPGLYACG
CCCCCCCCCCCHHHHHHHHHCCCCHHHHEEECCCCCCEECCCEEEECCCCCCCCCEEECC
EVSSTGLHGANRLASNSLLEGLAYARFIADGLDAPPPAAIVPEAVSARAAGDLPAIRALM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHH
ESRVGVVRDAGGLAEAVAVLEPGAETCDAALVALLIARGALARRESRGAHWRSDFPHLAP
HHCCCEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AAHTEATLFDAIAAPRATPSTEEVLTP
CCCCHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure
MNAVSPNNADRVVVVGGGVAGLSVALRLAPRPVTLVTASALGLGTATGWAQGGIAAAVGA
CCCCCCCCCCEEEEEECCHHHHHHEEEECCCCEEEEEEHHHCCCCCCCCCCCCEEEEECC
DDAAALHAADTEAAGAGLTEPDVALRVAGEGPALIDWLVRIGVPFDRTDDAALALGLEAA
CCHHEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEEHHHH
HGRRRIVRSGGDATGARVLEGLVRAVLEAPSVEIVTARACDLLRDTNGAVAGIVAERDGA
CCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCEEEEEEECCCC
LFRIPARAVVLATGGVGALYAATTNPPGAVGRGLALAARAGAVMRDMEFVQFHPTAIAAG
EEEECCEEEEEEECCCCEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHEECCCEEECC
ADPMPLATEALRGEGARLIDETGAAVMAGIAGGDLAPRDVVARGIFQALQRGRTVYLDAR
CCCCCHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECC
GALGAAMPTRFPTVAGLCAAAGIDPAVQAIPVRPAAHYHMGGVKVDAHGATSVPGLYACG
CCCCCCCCCCCHHHHHHHHHCCCCHHHHEEECCCCCCEECCCEEEECCCCCCCCCEEECC
EVSSTGLHGANRLASNSLLEGLAYARFIADGLDAPPPAAIVPEAVSARAAGDLPAIRALM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHH
ESRVGVVRDAGGLAEAVAVLEPGAETCDAALVALLIARGALARRESRGAHWRSDFPHLAP
HHCCCEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AAHTEATLFDAIAAPRATPSTEEVLTP
CCCCHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]