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The map label for this gene is gudD [H]

Identifier: 170751532

GI number: 170751532

Start: 5478973

End: 5480358

Strand: Direct

Name: gudD [H]

Synonym: Mrad2831_5152

Alternate gene names: 170751532

Gene position: 5478973-5480358 (Clockwise)

Preceding gene: 170751531

Following gene: 170751541

Centisome position: 90.15

GC content: 72.66

Gene sequence:

>1386_bases
ATGGATCACGCCACGGATCACGCCACGGATCACGCCATGTCGAACGAGAGCCGCCACGCCGCCCGCCACACGCCGACCGT
GACCGCCATGCGTGTCGTGCCGGTGGCCGGCCACGACAGCATGCTCCTCAACCTCTCGGGCGCGCACGGGCCGTACTTCA
CCCGCAACCTCGTGATCCTGACCGACTCCACCGGCGCCACCGGCCTCGGCGAGGTCCCCGGCGGCGAGGGCATCCGCAGA
ACCCTGGAGGATGCCCGGGACCTCGTGATCGGGCGGCCGCTCGGCGCCTGGAACGCGGTGCTCAACGCCATGCGGACCCG
CTTCGCCGACCGCGACGCGGGCGGCCGCGGCCTCCAGACCTTCGACCTGCGCATCACCATCCACGCGGTGACGGCGGTCG
AGTCCGCCTTCCTCGACCTGCTCGGCCAGTACCTCGACGTGCCGGTGGCCGCGCTCCTGGGCGAGGGCCAGCAGCGCGAC
GCGGTCGAGATGCTGGGCTACCTGTTCTACGTCGGCGACCGCACCCGCACGGATCTCGGCTACCGGGCGCCGAGCGCCCG
CGACGGCTGGTTCCGCCTGCGCGACGAGGAGGCGCTGACCCCCGAGGCCGTGGCCCGGCTCGCCGGGGCCGCCTACGACC
AGTACGGCTTCAACGACTTCAAGCTGAAGGGCGGCGTGCTGCGCGGCGAGGACGAGATCGCCGCCGTCACCGCGATCCAC
GAGCGGTTCCCCAAGGCCCGCGTGACGCTCGACCCGAACGGCGCGTGGTCCCTCGACGAGGCGATCCGCCTCTGCAAGGG
CCAGGGCCACGTCCTGGCCTACGCGGAGGATCCCTGCGGCGCCGAGGGCGGGTTCTCGGGCCGCGAGATCATGGCCGAGT
TCCGCCGCGCCACCGGCCTGCCCACCGCGACCAACATGATCGCCACCGACTGGCGGCAGATGAACCACGCGATCCAGCTC
GGCGCCGTCGACATCCCGCTGGCGGACCCGCACTTCTGGACGCTCGCCGGCTCGGTCCGGGTGGCCCAGCTGTGCCGGGA
CCACGGCCTGACCTGGGGCTCGCACTCGAACAACCACTTCGACGTGTCGCTCGCGATGTTCACCCACGTGGCCGCGGCGG
CGCCGGGCAACGTCACGGCGATCGACACCCACTGGATCTGGCAGGACGGCGAGCGCCTGACCAAGGAGCCTCTGGCGATC
CGCGGCGGCCTCGTGCGGGTGCCGGAGCGGCCGGGCCTCGGCATCGAGCTCGACATGGCGGCGGTGGAGGCCGCCCACGC
CCTCTACCGCGACCACGGCCTCGGCGCCCGCGACGACGCGGCCGCCATGCAGTTCCTGATCCCCGGCTGGACCTTCGACA
ACAAGAAGCCCTGCCTGGTCCGCTGA

Upstream 100 bases:

>100_bases
CACTGACCCGCCCACCGGGCCGGCCCCGTCCGGACCGGCCCCGCATCGATCCCGCCCCGCGCCCGGCGAAGACCGGCCGG
GGGCCCCCGAGGAGGACGCC

Downstream 100 bases:

>100_bases
GGACCGGCCCGGGCCCGCGCCGTCAGACGCGCGAGCTCGGGCGGTAGAACGGCTCGTGCCGCGACGGGTCGATGTAGTCG
GCGTAGAAGCGCCGCGCGTG

Product: glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MDHATDHATDHAMSNESRHAARHTPTVTAMRVVPVAGHDSMLLNLSGAHGPYFTRNLVILTDSTGATGLGEVPGGEGIRR
TLEDARDLVIGRPLGAWNAVLNAMRTRFADRDAGGRGLQTFDLRITIHAVTAVESAFLDLLGQYLDVPVAALLGEGQQRD
AVEMLGYLFYVGDRTRTDLGYRAPSARDGWFRLRDEEALTPEAVARLAGAAYDQYGFNDFKLKGGVLRGEDEIAAVTAIH
ERFPKARVTLDPNGAWSLDEAIRLCKGQGHVLAYAEDPCGAEGGFSGREIMAEFRRATGLPTATNMIATDWRQMNHAIQL
GAVDIPLADPHFWTLAGSVRVAQLCRDHGLTWGSHSNNHFDVSLAMFTHVAAAAPGNVTAIDTHWIWQDGERLTKEPLAI
RGGLVRVPERPGLGIELDMAAVEAAHALYRDHGLGARDDAAAMQFLIPGWTFDNKKPCLVR

Sequences:

>Translated_461_residues
MDHATDHATDHAMSNESRHAARHTPTVTAMRVVPVAGHDSMLLNLSGAHGPYFTRNLVILTDSTGATGLGEVPGGEGIRR
TLEDARDLVIGRPLGAWNAVLNAMRTRFADRDAGGRGLQTFDLRITIHAVTAVESAFLDLLGQYLDVPVAALLGEGQQRD
AVEMLGYLFYVGDRTRTDLGYRAPSARDGWFRLRDEEALTPEAVARLAGAAYDQYGFNDFKLKGGVLRGEDEIAAVTAIH
ERFPKARVTLDPNGAWSLDEAIRLCKGQGHVLAYAEDPCGAEGGFSGREIMAEFRRATGLPTATNMIATDWRQMNHAIQL
GAVDIPLADPHFWTLAGSVRVAQLCRDHGLTWGSHSNNHFDVSLAMFTHVAAAAPGNVTAIDTHWIWQDGERLTKEPLAI
RGGLVRVPERPGLGIELDMAAVEAAHALYRDHGLGARDDAAAMQFLIPGWTFDNKKPCLVR
>Mature_461_residues
MDHATDHATDHAMSNESRHAARHTPTVTAMRVVPVAGHDSMLLNLSGAHGPYFTRNLVILTDSTGATGLGEVPGGEGIRR
TLEDARDLVIGRPLGAWNAVLNAMRTRFADRDAGGRGLQTFDLRITIHAVTAVESAFLDLLGQYLDVPVAALLGEGQQRD
AVEMLGYLFYVGDRTRTDLGYRAPSARDGWFRLRDEEALTPEAVARLAGAAYDQYGFNDFKLKGGVLRGEDEIAAVTAIH
ERFPKARVTLDPNGAWSLDEAIRLCKGQGHVLAYAEDPCGAEGGFSGREIMAEFRRATGLPTATNMIATDWRQMNHAIQL
GAVDIPLADPHFWTLAGSVRVAQLCRDHGLTWGSHSNNHFDVSLAMFTHVAAAAPGNVTAIDTHWIWQDGERLTKEPLAI
RGGLVRVPERPGLGIELDMAAVEAAHALYRDHGLGARDDAAAMQFLIPGWTFDNKKPCLVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=440, Percent_Identity=71.3636363636364, Blast_Score=670, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=447, Percent_Identity=62.6398210290828, Blast_Score=548, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 50104; Mature: 50104

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHATDHATDHAMSNESRHAARHTPTVTAMRVVPVAGHDSMLLNLSGAHGPYFTRNLVIL
CCCCCCCCHHHHCCCCCHHHHHCCCCEEEEEEEEECCCCEEEEEECCCCCCEEECEEEEE
TDSTGATGLGEVPGGEGIRRTLEDARDLVIGRPLGAWNAVLNAMRTRFADRDAGGRGLQT
ECCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEE
FDLRITIHAVTAVESAFLDLLGQYLDVPVAALLGEGQQRDAVEMLGYLFYVGDRTRTDLG
EEEEEEEEEHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCCC
YRAPSARDGWFRLRDEEALTPEAVARLAGAAYDQYGFNDFKLKGGVLRGEDEIAAVTAIH
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCEEECCCHHHHHHHHH
ERFPKARVTLDPNGAWSLDEAIRLCKGQGHVLAYAEDPCGAEGGFSGREIMAEFRRATGL
HHCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCC
PTATNMIATDWRQMNHAIQLGAVDIPLADPHFWTLAGSVRVAQLCRDHGLTWGSHSNNHF
CCHHHHHHHHHHHHHHEEEECEEECCCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCEE
DVSLAMFTHVAAAAPGNVTAIDTHWIWQDGERLTKEPLAIRGGLVRVPERPGLGIELDMA
EEEHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHCCCHHHCCCEEECCCCCCCCEEEHHH
AVEAAHALYRDHGLGARDDAAAMQFLIPGWTFDNKKPCLVR
HHHHHHHHHHHCCCCCCCCHHHHHEEECCEECCCCCCCCCC
>Mature Secondary Structure
MDHATDHATDHAMSNESRHAARHTPTVTAMRVVPVAGHDSMLLNLSGAHGPYFTRNLVIL
CCCCCCCCHHHHCCCCCHHHHHCCCCEEEEEEEEECCCCEEEEEECCCCCCEEECEEEEE
TDSTGATGLGEVPGGEGIRRTLEDARDLVIGRPLGAWNAVLNAMRTRFADRDAGGRGLQT
ECCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEE
FDLRITIHAVTAVESAFLDLLGQYLDVPVAALLGEGQQRDAVEMLGYLFYVGDRTRTDLG
EEEEEEEEEHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCCC
YRAPSARDGWFRLRDEEALTPEAVARLAGAAYDQYGFNDFKLKGGVLRGEDEIAAVTAIH
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCEEECCCHHHHHHHHH
ERFPKARVTLDPNGAWSLDEAIRLCKGQGHVLAYAEDPCGAEGGFSGREIMAEFRRATGL
HHCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCC
PTATNMIATDWRQMNHAIQLGAVDIPLADPHFWTLAGSVRVAQLCRDHGLTWGSHSNNHF
CCHHHHHHHHHHHHHHEEEECEEECCCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCEE
DVSLAMFTHVAAAAPGNVTAIDTHWIWQDGERLTKEPLAIRGGLVRVPERPGLGIELDMA
EEEHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHCCCHHHCCCEEECCCCCCCCEEEHHH
AVEAAHALYRDHGLGARDDAAAMQFLIPGWTFDNKKPCLVR
HHHHHHHHHHHCCCCCCCCHHHHHEEECCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9772161 [H]