Click here to switch to the map view.

The map label for this gene is ynfL [H]

Identifier: 170751529

GI number: 170751529

Start: 5475500

End: 5476402

Strand: Reverse

Name: ynfL [H]

Synonym: Mrad2831_5149

Alternate gene names: 170751529

Gene position: 5476402-5475500 (Counterclockwise)

Preceding gene: 170751533

Following gene: 170751528

Centisome position: 90.1

GC content: 74.86

Gene sequence:

>903_bases
ATGTTCGATCTCGGCCACGCCCGCAGCTTCGTCGCCGTCGCCGAGGAGATGCATTTCGGCCGGGCCGCGACGCGGCTGAA
CCTGACCCAATCGCCCCTGTCGCGGCAGATCCAGATGCTGGAGCAGACGCTGGGCGTGACGCTGCTGGAGCGGACCACCC
GGGCGGTGCGCCTGACCCCGGCGGGCCGCACCTTCCTGGCCGAGGCCTACCGGGTGCTGGCGGCGGCCGAGAACGCCGCC
CGCATCACGCGCCGCGCCGCTCGGGGCGAGAGCGGGGTGCTGCGCCTCAGCTTCACCGCCGCCTCGGCCTACCGGACGCT
GCCGCAGGTCGTCGCCCATGTCCGCCGGACGCTGCCCGAGGTCGACCTCGTGCTGGAGGAGATGGTCTCGGACGAGCAGA
TCCTGGCGCTGGGCGAGAACCGCACGGATCTCGCGATCCTGCGGCCGGCCCCGGTCCTGCAGGATCCGAAATCCGCGATC
GCCACCGCGCCGCTGGCGCGCGAGCGGCTCCTCCTCGCGGTCCCGCGCGGGCACCGCCTCGCCGCGGCGCGCGCGCCGAC
CCTGAGGGACCTCGACGGCGAGGCCTTCGTGACGTGGTCGCCCCGGGGCGGCCGCTACTTCATCGACCTGCTCGACCGTC
TGTTCGAGGCCGGGGGCGTGCAGCCCCGGATCGTGCAGCGGGTGAACCAGGTCCACGCGATGCTGGCCCTCGTCGGCGCC
GGCATCGGCGTCGCGCTGGTGCCGGGCAGCGCCCGGGGCAACAGCATGGCCGACATCCGGCTCCGGCCGATCGCCCTGCC
GGCCGCGACCGGACCGGAGCTGTGGCTCGCGTGGCGCAGCGACAACGCCAATCCGTGCCTGCCCTCGGTGCGGGCCGCGG
CGCTGGAGACCGGCGAGGCGTGA

Upstream 100 bases:

>100_bases
CTCCCTGGAACGCACCGTTCGGTGTCGCGTCCGGGGTTAGGCGCCGGCGTTAAATGCGTCCAACGTTGATTTTGGCTGGA
TTATCCCGGATCCTGCATCA

Downstream 100 bases:

>100_bases
CCGCCGGCGCCGCCCTGGAAAGTCGCCGCCGCCGGCGATAGGCTGCCCGCTCGCCCCGCTTCCGGTCGCGCTGCCGGTCG
GGGCCGCCCCTCCCGGATCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MFDLGHARSFVAVAEEMHFGRAATRLNLTQSPLSRQIQMLEQTLGVTLLERTTRAVRLTPAGRTFLAEAYRVLAAAENAA
RITRRAARGESGVLRLSFTAASAYRTLPQVVAHVRRTLPEVDLVLEEMVSDEQILALGENRTDLAILRPAPVLQDPKSAI
ATAPLARERLLLAVPRGHRLAAARAPTLRDLDGEAFVTWSPRGGRYFIDLLDRLFEAGGVQPRIVQRVNQVHAMLALVGA
GIGVALVPGSARGNSMADIRLRPIALPAATGPELWLAWRSDNANPCLPSVRAAALETGEA

Sequences:

>Translated_300_residues
MFDLGHARSFVAVAEEMHFGRAATRLNLTQSPLSRQIQMLEQTLGVTLLERTTRAVRLTPAGRTFLAEAYRVLAAAENAA
RITRRAARGESGVLRLSFTAASAYRTLPQVVAHVRRTLPEVDLVLEEMVSDEQILALGENRTDLAILRPAPVLQDPKSAI
ATAPLARERLLLAVPRGHRLAAARAPTLRDLDGEAFVTWSPRGGRYFIDLLDRLFEAGGVQPRIVQRVNQVHAMLALVGA
GIGVALVPGSARGNSMADIRLRPIALPAATGPELWLAWRSDNANPCLPSVRAAALETGEA
>Mature_300_residues
MFDLGHARSFVAVAEEMHFGRAATRLNLTQSPLSRQIQMLEQTLGVTLLERTTRAVRLTPAGRTFLAEAYRVLAAAENAA
RITRRAARGESGVLRLSFTAASAYRTLPQVVAHVRRTLPEVDLVLEEMVSDEQILALGENRTDLAILRPAPVLQDPKSAI
ATAPLARERLLLAVPRGHRLAAARAPTLRDLDGEAFVTWSPRGGRYFIDLLDRLFEAGGVQPRIVQRVNQVHAMLALVGA
GIGVALVPGSARGNSMADIRLRPIALPAATGPELWLAWRSDNANPCLPSVRAAALETGEA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=278, Percent_Identity=39.568345323741, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI2367136, Length=293, Percent_Identity=32.0819112627986, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788887, Length=288, Percent_Identity=32.6388888888889, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788748, Length=268, Percent_Identity=28.7313432835821, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI145693105, Length=252, Percent_Identity=28.1746031746032, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790399, Length=243, Percent_Identity=30.0411522633745, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI157672245, Length=233, Percent_Identity=32.1888412017167, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787806, Length=248, Percent_Identity=27.8225806451613, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI87081904, Length=203, Percent_Identity=33.9901477832512, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790262, Length=248, Percent_Identity=25.4032258064516, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790208, Length=277, Percent_Identity=28.8808664259928, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786401, Length=165, Percent_Identity=35.1515151515151, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693193, Length=247, Percent_Identity=27.5303643724696, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788296, Length=237, Percent_Identity=27.8481012658228, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787530, Length=241, Percent_Identity=24.896265560166, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32460; Mature: 32460

Theoretical pI: Translated: 11.16; Mature: 11.16

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDLGHARSFVAVAEEMHFGRAATRLNLTQSPLSRQIQMLEQTLGVTLLERTTRAVRLTP
CCCCCHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
AGRTFLAEAYRVLAAAENAARITRRAARGESGVLRLSFTAASAYRTLPQVVAHVRRTLPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCH
VDLVLEEMVSDEQILALGENRTDLAILRPAPVLQDPKSAIATAPLARERLLLAVPRGHRL
HHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCCCHHHHHHCCHHHCCEEEECCCCCEE
AAARAPTLRDLDGEAFVTWSPRGGRYFIDLLDRLFEAGGVQPRIVQRVNQVHAMLALVGA
ECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GIGVALVPGSARGNSMADIRLRPIALPAATGPELWLAWRSDNANPCLPSVRAAALETGEA
CCCEEEECCCCCCCCEEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MFDLGHARSFVAVAEEMHFGRAATRLNLTQSPLSRQIQMLEQTLGVTLLERTTRAVRLTP
CCCCCHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
AGRTFLAEAYRVLAAAENAARITRRAARGESGVLRLSFTAASAYRTLPQVVAHVRRTLPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCH
VDLVLEEMVSDEQILALGENRTDLAILRPAPVLQDPKSAIATAPLARERLLLAVPRGHRL
HHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCCCHHHHHHCCHHHCCEEEECCCCCEE
AAARAPTLRDLDGEAFVTWSPRGGRYFIDLLDRLFEAGGVQPRIVQRVNQVHAMLALVGA
ECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GIGVALVPGSARGNSMADIRLRPIALPAATGPELWLAWRSDNANPCLPSVRAAALETGEA
CCCEEEECCCCCCCCEEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]