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The map label for this gene is flbD [H]

Identifier: 170751436

GI number: 170751436

Start: 5369853

End: 5371214

Strand: Reverse

Name: flbD [H]

Synonym: Mrad2831_5055

Alternate gene names: 170751436

Gene position: 5371214-5369853 (Counterclockwise)

Preceding gene: 170751437

Following gene: 170751433

Centisome position: 88.37

GC content: 73.13

Gene sequence:

>1362_bases
ATGCGGCTCCTGATGATCGGGCGGCTCAACGGCGAGCTGATCACCGCCTCGAAGATCGCCATGCAGCGCGGCGCCGCCGT
CACGCAGGCCGACGCCGTTCCGCAGGGCCTCACCGTCCTGCGCGCGCGGGGCGCCGACCTCATCATGATCGACGTGGGGC
TGCCGATCCGGGAGCTCGTCACCGCGTTGGAGGGGGAGCGGATCCGCACGCCGGTGGTCGCCTGCGGCGTCTCGGCGGAC
GCCTCAGCCGCCGTGGCGGCGATCCGGGCGGGCGCCAAGGAATACATCCCGCTCCCGCCCGATCCCGAGATGATCGCGGC
GGTGCTGGAGGCAGTCGCCACCGACCGCGCCGCCTTCGTCTGGCGCGACCCGTCCATGGAGCGGGTGGTCAAGCTCGCCG
AGCAGGTCGCCCGCTCCGAGGCCTCGGTGCTGATCACCGGCGAGAGCGGCACCGGCAAGGAGGTGCTGGCCCGGCACGTC
CACCAGAAGTCCAACCGGGCGGGCAAGCCCTTCGTCTCGGTGAACTGCGCGGCGATCCCCGAGGCCCTGCTCGAGTCCGA
GCTGTTCGGCCACGAGAAGGGCGCGTTCACCGGCGCGGTCGCCCGACGCATCGGCCGGTTCGAGGAGGCCAACGGCGGCA
CGCTGCTCCTCGACGAGATCTCCGAGATGGACGTGCGCCTGCAGGCCAAGCTGCTGCGCGCCCTGCAGGAGCGGGTGATC
GACCGGGTCGGCGGCACCGCCCCGGTCCGGGTCGACATCCGCGTGCTCGCCACCTCGAACCGCAACCTCGCCGAGGAGGT
CCGCAAGGGCACGTTCCGCGAGGACCTGCTCTACCGCCTCAACGTCGTCGGGCTGCGGCTCCCGCCGCTGCGCGAGCGAC
CGGCCGACATCCTCGAACTCGCCACTCACTTCGCCCGGAAATACGCCGCCGTGAACGGGATGCCGGTCCGCCCGCTCTCC
GCGGAGGCCCGGGCGCTCGTGCTGCGGAACCCGTGGCGCGGCAACGTGCGCGAGCTGGAGAACACCCTGCACCGGGCGGT
GCTGCTCGCCTCGGGCGACGCGATCGGCCCGGAGGCGATCCTCTCGCCCGAGGGCGAGACCTTCGCGGCGGAGGGGCCGG
CCGCGCGCGCCGCGCAGGTCGCCGAGGCCGCCACCCGCGGGCTGGTGGGCCGGACCGTGGCGCAGGTCGAGTGCGACCTG
ATCCTCGACACGCTGGACCATTGCCTCGGCAACCGGACCCACGCGGCCAAGATCCTCGGCATCTCGATCCGGACCCTGCG
CAACAAGCTCAACGAGTACGTCTCGGCCGGTCTCGACGTGGCCGAGCCCGGCAGCGTCCGGGCGAGCGTCGCCTGCGGCT
GA

Upstream 100 bases:

>100_bases
CCGCCTCGGCGTGACGATGACCGAGATCGTCAAGGGTGAGTGATTGTTCGGTGCGGATGCCGGGGGGCTGTCCGCGCCCG
GGTTTTGAGAGGGACGAACC

Downstream 100 bases:

>100_bases
CGCGCGTGTCCCTGTAGGGGCTTCGGGATCGGCTTCACCCCGCCATCCCGGGGCCGGGCGGCGGAGCCCGGGATCCCGAC
GCGCCAGCGCAGGGCGGACC

Product: two component sigma-54 specific Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MRLLMIGRLNGELITASKIAMQRGAAVTQADAVPQGLTVLRARGADLIMIDVGLPIRELVTALEGERIRTPVVACGVSAD
ASAAVAAIRAGAKEYIPLPPDPEMIAAVLEAVATDRAAFVWRDPSMERVVKLAEQVARSEASVLITGESGTGKEVLARHV
HQKSNRAGKPFVSVNCAAIPEALLESELFGHEKGAFTGAVARRIGRFEEANGGTLLLDEISEMDVRLQAKLLRALQERVI
DRVGGTAPVRVDIRVLATSNRNLAEEVRKGTFREDLLYRLNVVGLRLPPLRERPADILELATHFARKYAAVNGMPVRPLS
AEARALVLRNPWRGNVRELENTLHRAVLLASGDAIGPEAILSPEGETFAAEGPAARAAQVAEAATRGLVGRTVAQVECDL
ILDTLDHCLGNRTHAAKILGISIRTLRNKLNEYVSAGLDVAEPGSVRASVACG

Sequences:

>Translated_453_residues
MRLLMIGRLNGELITASKIAMQRGAAVTQADAVPQGLTVLRARGADLIMIDVGLPIRELVTALEGERIRTPVVACGVSAD
ASAAVAAIRAGAKEYIPLPPDPEMIAAVLEAVATDRAAFVWRDPSMERVVKLAEQVARSEASVLITGESGTGKEVLARHV
HQKSNRAGKPFVSVNCAAIPEALLESELFGHEKGAFTGAVARRIGRFEEANGGTLLLDEISEMDVRLQAKLLRALQERVI
DRVGGTAPVRVDIRVLATSNRNLAEEVRKGTFREDLLYRLNVVGLRLPPLRERPADILELATHFARKYAAVNGMPVRPLS
AEARALVLRNPWRGNVRELENTLHRAVLLASGDAIGPEAILSPEGETFAAEGPAARAAQVAEAATRGLVGRTVAQVECDL
ILDTLDHCLGNRTHAAKILGISIRTLRNKLNEYVSAGLDVAEPGSVRASVACG
>Mature_453_residues
MRLLMIGRLNGELITASKIAMQRGAAVTQADAVPQGLTVLRARGADLIMIDVGLPIRELVTALEGERIRTPVVACGVSAD
ASAAVAAIRAGAKEYIPLPPDPEMIAAVLEAVATDRAAFVWRDPSMERVVKLAEQVARSEASVLITGESGTGKEVLARHV
HQKSNRAGKPFVSVNCAAIPEALLESELFGHEKGAFTGAVARRIGRFEEANGGTLLLDEISEMDVRLQAKLLRALQERVI
DRVGGTAPVRVDIRVLATSNRNLAEEVRKGTFREDLLYRLNVVGLRLPPLRERPADILELATHFARKYAAVNGMPVRPLS
AEARALVLRNPWRGNVRELENTLHRAVLLASGDAIGPEAILSPEGETFAAEGPAARAAQVAEAATRGLVGRTVAQVECDL
ILDTLDHCLGNRTHAAKILGISIRTLRNKLNEYVSAGLDVAEPGSVRASVACG

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=419, Percent_Identity=43.436754176611, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1790437, Length=430, Percent_Identity=39.7674418604651, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI1790299, Length=426, Percent_Identity=38.9671361502347, Blast_Score=271, Evalue=8e-74,
Organism=Escherichia coli, GI87082117, Length=237, Percent_Identity=52.3206751054852, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1788905, Length=422, Percent_Identity=37.6777251184834, Blast_Score=248, Evalue=8e-67,
Organism=Escherichia coli, GI1787583, Length=324, Percent_Identity=42.5925925925926, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI87082152, Length=257, Percent_Identity=50.5836575875486, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1789087, Length=262, Percent_Identity=47.7099236641221, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1789233, Length=312, Percent_Identity=41.3461538461538, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1786524, Length=334, Percent_Identity=42.5149700598802, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87081872, Length=314, Percent_Identity=39.171974522293, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1789828, Length=244, Percent_Identity=38.1147540983607, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI87081858, Length=350, Percent_Identity=27.7142857142857, Blast_Score=125, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48438; Mature: 48438

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLMIGRLNGELITASKIAMQRGAAVTQADAVPQGLTVLRARGADLIMIDVGLPIRELV
CEEEEEECCCCCEEEHHHHHHHCCCCHHHHHCCCCCHHHHHCCCCCEEEEECCCCHHHHH
TALEGERIRTPVVACGVSADASAAVAAIRAGAKEYIPLPPDPEMIAAVLEAVATDRAAFV
HHHCCCCCCCCEEEECCCCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCCCEEEE
WRDPSMERVVKLAEQVARSEASVLITGESGTGKEVLARHVHQKSNRAGKPFVSVNCAAIP
ECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHH
EALLESELFGHEKGAFTGAVARRIGRFEEANGGTLLLDEISEMDVRLQAKLLRALQERVI
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
DRVGGTAPVRVDIRVLATSNRNLAEEVRKGTFREDLLYRLNVVGLRLPPLRERPADILEL
HHHCCCCCEEEEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHEEECCCCCCCCHHHHHH
ATHFARKYAAVNGMPVRPLSAEARALVLRNPWRGNVRELENTLHRAVLLASGDAIGPEAI
HHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHEECCCCCCCCCE
LSPEGETFAAEGPAARAAQVAEAATRGLVGRTVAQVECDLILDTLDHCLGNRTHAAKILG
ECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
ISIRTLRNKLNEYVSAGLDVAEPGSVRASVACG
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCC
>Mature Secondary Structure
MRLLMIGRLNGELITASKIAMQRGAAVTQADAVPQGLTVLRARGADLIMIDVGLPIRELV
CEEEEEECCCCCEEEHHHHHHHCCCCHHHHHCCCCCHHHHHCCCCCEEEEECCCCHHHHH
TALEGERIRTPVVACGVSADASAAVAAIRAGAKEYIPLPPDPEMIAAVLEAVATDRAAFV
HHHCCCCCCCCEEEECCCCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCCCEEEE
WRDPSMERVVKLAEQVARSEASVLITGESGTGKEVLARHVHQKSNRAGKPFVSVNCAAIP
ECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHH
EALLESELFGHEKGAFTGAVARRIGRFEEANGGTLLLDEISEMDVRLQAKLLRALQERVI
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
DRVGGTAPVRVDIRVLATSNRNLAEEVRKGTFREDLLYRLNVVGLRLPPLRERPADILEL
HHHCCCCCEEEEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHEEECCCCCCCCHHHHHH
ATHFARKYAAVNGMPVRPLSAEARALVLRNPWRGNVRELENTLHRAVLLASGDAIGPEAI
HHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHEECCCCCCCCCE
LSPEGETFAAEGPAARAAQVAEAATRGLVGRTVAQVECDLILDTLDHCLGNRTHAAKILG
ECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
ISIRTLRNKLNEYVSAGLDVAEPGSVRASVACG
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]