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The map label for this gene is nudG [H]

Identifier: 170751322

GI number: 170751322

Start: 5259578

End: 5260018

Strand: Reverse

Name: nudG [H]

Synonym: Mrad2831_4940

Alternate gene names: 170751322

Gene position: 5260018-5259578 (Counterclockwise)

Preceding gene: 170751323

Following gene: 170751315

Centisome position: 86.54

GC content: 73.24

Gene sequence:

>441_bases
GTGAGCGCGCCCGATAGCGCCCCTGCGGCCGCGCCCCTGCGCCTGCTCCTCGTGGTCGCCGTCGCCCTCGTCGACGTCGA
CGGCCGCGTCCTCGTGAGCGAGCGCCCGGCGGGCAAGCAGCTCGCCGGTCTCTGGGAGTTCCCCGGCGGCAAGGTCGAGC
CCGGCGAGCGTCCCGAGCAGACGCTGATCCGGGAGCTCGCCGAGGAACTCGGCATCCGGGTCGAGGAGCCGTGTCTCGCG
CCGCTCACCTTCGCCAGCCACGCCTATCCGGACTTCCACCTTCTGATGCCGCTCTACGTCTGCCGGCGCTGGACCGGCAC
GCCGCGCCCGATGGAGGGGCAGGCGCTCAGATGGGTCCGGCCGAAGGCGCTGCGCGATCTCGCCATGCCGCCGGCCGACG
CGCCGCTGATCCCGTTCCTGATCGACCTGCTCGAGGTCTGA

Upstream 100 bases:

>100_bases
CCCGCGCGGCCCCGATGGCCGCCAAGGGGCGGCGGGTGCCCCTCGAGGCGGGCACGATCCAGATGTCCTCCGAGGTCTCG
GCCACCTTCGCGGTGGCGCA

Downstream 100 bases:

>100_bases
GAGGGTATCCGGCTGAAGCGACCGGGTGACGGAAACGCTCCGTCGTCGCGAGCGCAGCGAAGCAACTCAGGGCAGCGCGA
CATCAGCCGGCGTCGCGCCG

Product: NUDIX hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 145

Protein sequence:

>146_residues
MSAPDSAPAAAPLRLLLVVAVALVDVDGRVLVSERPAGKQLAGLWEFPGGKVEPGERPEQTLIRELAEELGIRVEEPCLA
PLTFASHAYPDFHLLMPLYVCRRWTGTPRPMEGQALRWVRPKALRDLAMPPADAPLIPFLIDLLEV

Sequences:

>Translated_146_residues
MSAPDSAPAAAPLRLLLVVAVALVDVDGRVLVSERPAGKQLAGLWEFPGGKVEPGERPEQTLIRELAEELGIRVEEPCLA
PLTFASHAYPDFHLLMPLYVCRRWTGTPRPMEGQALRWVRPKALRDLAMPPADAPLIPFLIDLLEV
>Mature_145_residues
SAPDSAPAAAPLRLLLVVAVALVDVDGRVLVSERPAGKQLAGLWEFPGGKVEPGERPEQTLIRELAEELGIRVEEPCLAP
LTFASHAYPDFHLLMPLYVCRRWTGTPRPMEGQALRWVRPKALRDLAMPPADAPLIPFLIDLLEV

Specific function: Specific for pyrimidine substrates. Acts on 5-methyl- dCTP, CTP and dCTP in decreasing order [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=141, Percent_Identity=37.5886524822695, Blast_Score=82, Evalue=1e-17,
Organism=Escherichia coli, GI1786288, Length=122, Percent_Identity=35.2459016393443, Blast_Score=81, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.-

Molecular weight: Translated: 16034; Mature: 15903

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPDSAPAAAPLRLLLVVAVALVDVDGRVLVSERPAGKQLAGLWEFPGGKVEPGERPEQ
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCCCCCCCCCHHH
TLIRELAEELGIRVEEPCLAPLTFASHAYPDFHLLMPLYVCRRWTGTPRPMEGQALRWVR
HHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCC
PKALRDLAMPPADAPLIPFLIDLLEV
HHHHHHHCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SAPDSAPAAAPLRLLLVVAVALVDVDGRVLVSERPAGKQLAGLWEFPGGKVEPGERPEQ
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCCCCCCCCCHHH
TLIRELAEELGIRVEEPCLAPLTFASHAYPDFHLLMPLYVCRRWTGTPRPMEGQALRWVR
HHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCC
PKALRDLAMPPADAPLIPFLIDLLEV
HHHHHHHCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 11053429 [H]