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The map label for this gene is yafC [H]

Identifier: 170751315

GI number: 170751315

Start: 5252115

End: 5253083

Strand: Reverse

Name: yafC [H]

Synonym: Mrad2831_4932

Alternate gene names: 170751315

Gene position: 5253083-5252115 (Counterclockwise)

Preceding gene: 170751322

Following gene: 170751314

Centisome position: 86.43

GC content: 74.51

Gene sequence:

>969_bases
ATGGAAACCTATCGTTTCTGGAAATGGCTGATGAGACTGCCCGATTTCGAGGCCTGGGCCGTGTTCGCCAAGGTGGTGGA
GACCGGCTCATTCGGGCGCGCCGCCGCGGAGCTCGGCCTGTCGAAGGCCACGGTCTCGAAGGCGGTGAGCCGGCTCGAGC
TGCGGATCGGTGCCCGCCTGTTCAACCGCACATCGCGGAAGCTCGCCCTCACCGAGGCCGGGCAGGCCGCCAAGGACGGC
GCCGCGCGCATCCTGGCCGAGGGCGAGGCCGCGGAAGCCCTCGCCACGGCCGGTGCCGCGGAGCCGCGCGGGCTGGTGCG
GCTCGCAGCGCCGATGACCTTCGGGGTGATGCACGTGGCGCCGATCCTTCCGGCCTTCTTCCGGGAGTACCCGGCCGTGT
CGGTGGATCTCCACCTGTCGGACGCGGTCGTCGACCTCGTCGGCGGCGGGTTCGACATCGGGCTGCGCATCGCGGCGCTG
CCCGATTCCTCCCTGCGCGCGCGCCGTCTCTGCGAGATCCGCCGCTCGCTGGTCGCCACCCCCGCCTATCTCGACCGCCA
CGGCCGGCCCGAGCACCCGGACGACCTCGTCCGCCACACCTGCCTCGGCTACGCCTACCTGCCGACGCCCGACCGCTGGC
GATTCGTGGACGGGAGCGGCGCGGAGGCCGTCGTGGTGCCGGCGGGCCAGCTGCGGGCGAACAACGCCGAGGCGCTGGCG
CCGGCGCTGCGGGCCGGGCTCGGCCTCGCCCTGCAGCCCGACTTCATGATCTGGGACGACCTCGAATCCGGGCGCCTCGA
GCGGGTGCTGCCGGCCTGGTCGCCGCCGCCGATCGCCCTGCACCTCGTGACGCCCCCGGGCGACCCGAGGCCGGCCCGGG
TGACGGCGCTGATCGCCCATCTCGGCCAGGCCCTCGGCCAGGCGCCCTGGGCCGCTCCGCAGCCGCGGCGCGACCCGGCG
GACCGGTGA

Upstream 100 bases:

>100_bases
GTCTGAACCATGGCGATGTCTCCCGACGGAAGGCCGCGACGGGCGGCCTGTTGCGATGACGGGAATGTGCCCCTTGCCAT
GAGCCGCGAAAATAGAAACG

Downstream 100 bases:

>100_bases
GCCGCGCCGGCGCCCTCCCCGCCGCCGGCCCCGATCCGTGCGCGTCACTCACCTGGATCAGTGTCGGGGTCCTGCGCCTG
CGCTATCGGCGCGTCGCACG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
METYRFWKWLMRLPDFEAWAVFAKVVETGSFGRAAAELGLSKATVSKAVSRLELRIGARLFNRTSRKLALTEAGQAAKDG
AARILAEGEAAEALATAGAAEPRGLVRLAAPMTFGVMHVAPILPAFFREYPAVSVDLHLSDAVVDLVGGGFDIGLRIAAL
PDSSLRARRLCEIRRSLVATPAYLDRHGRPEHPDDLVRHTCLGYAYLPTPDRWRFVDGSGAEAVVVPAGQLRANNAEALA
PALRAGLGLALQPDFMIWDDLESGRLERVLPAWSPPPIALHLVTPPGDPRPARVTALIAHLGQALGQAPWAAPQPRRDPA
DR

Sequences:

>Translated_322_residues
METYRFWKWLMRLPDFEAWAVFAKVVETGSFGRAAAELGLSKATVSKAVSRLELRIGARLFNRTSRKLALTEAGQAAKDG
AARILAEGEAAEALATAGAAEPRGLVRLAAPMTFGVMHVAPILPAFFREYPAVSVDLHLSDAVVDLVGGGFDIGLRIAAL
PDSSLRARRLCEIRRSLVATPAYLDRHGRPEHPDDLVRHTCLGYAYLPTPDRWRFVDGSGAEAVVVPAGQLRANNAEALA
PALRAGLGLALQPDFMIWDDLESGRLERVLPAWSPPPIALHLVTPPGDPRPARVTALIAHLGQALGQAPWAAPQPRRDPA
DR
>Mature_322_residues
METYRFWKWLMRLPDFEAWAVFAKVVETGSFGRAAAELGLSKATVSKAVSRLELRIGARLFNRTSRKLALTEAGQAAKDG
AARILAEGEAAEALATAGAAEPRGLVRLAAPMTFGVMHVAPILPAFFREYPAVSVDLHLSDAVVDLVGGGFDIGLRIAAL
PDSSLRARRLCEIRRSLVATPAYLDRHGRPEHPDDLVRHTCLGYAYLPTPDRWRFVDGSGAEAVVVPAGQLRANNAEALA
PALRAGLGLALQPDFMIWDDLESGRLERVLPAWSPPPIALHLVTPPGDPRPARVTALIAHLGQALGQAPWAAPQPRRDPA
DR

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=301, Percent_Identity=34.8837209302326, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI1789639, Length=282, Percent_Identity=32.9787234042553, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1789440, Length=261, Percent_Identity=32.9501915708812, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1787128, Length=278, Percent_Identity=31.294964028777, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI145693193, Length=280, Percent_Identity=32.1428571428571, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87081978, Length=269, Percent_Identity=32.3420074349442, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1787589, Length=279, Percent_Identity=29.3906810035842, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1789173, Length=255, Percent_Identity=28.6274509803922, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790011, Length=277, Percent_Identity=25.6317689530686, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI157672245, Length=149, Percent_Identity=31.5436241610738, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787879, Length=150, Percent_Identity=35.3333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786448, Length=128, Percent_Identity=32.03125, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788748, Length=123, Percent_Identity=32.520325203252, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34752; Mature: 34752

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METYRFWKWLMRLPDFEAWAVFAKVVETGSFGRAAAELGLSKATVSKAVSRLELRIGARL
CCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FNRTSRKLALTEAGQAAKDGAARILAEGEAAEALATAGAAEPRGLVRLAAPMTFGVMHVA
HHCCCCEEEEHHCCCHHHCCHHHEEECCHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHH
PILPAFFREYPAVSVDLHLSDAVVDLVGGGFDIGLRIAALPDSSLRARRLCEIRRSLVAT
HHHHHHHHHCCCEEEEEEHHHHHHHHHCCCEEECEEEEECCCCHHHHHHHHHHHHHHHCC
PAYLDRHGRPEHPDDLVRHTCLGYAYLPTPDRWRFVDGSGAEAVVVPAGQLRANNAEALA
CHHHHCCCCCCCHHHHHHHHHHCEEECCCCCCEEEECCCCCCEEEECCCCCCCCCHHHHH
PALRAGLGLALQPDFMIWDDLESGRLERVLPAWSPPPIALHLVTPPGDPRPARVTALIAH
HHHHHCCCCEECCCEEEECCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHH
LGQALGQAPWAAPQPRRDPADR
HHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
METYRFWKWLMRLPDFEAWAVFAKVVETGSFGRAAAELGLSKATVSKAVSRLELRIGARL
CCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
FNRTSRKLALTEAGQAAKDGAARILAEGEAAEALATAGAAEPRGLVRLAAPMTFGVMHVA
HHCCCCEEEEHHCCCHHHCCHHHEEECCHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHH
PILPAFFREYPAVSVDLHLSDAVVDLVGGGFDIGLRIAALPDSSLRARRLCEIRRSLVAT
HHHHHHHHHCCCEEEEEEHHHHHHHHHCCCEEECEEEEECCCCHHHHHHHHHHHHHHHCC
PAYLDRHGRPEHPDDLVRHTCLGYAYLPTPDRWRFVDGSGAEAVVVPAGQLRANNAEALA
CHHHHCCCCCCCHHHHHHHHHHCEEECCCCCCEEEECCCCCCEEEECCCCCCCCCHHHHH
PALRAGLGLALQPDFMIWDDLESGRLERVLPAWSPPPIALHLVTPPGDPRPARVTALIAH
HHHHHCCCCEECCCEEEECCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHH
LGQALGQAPWAAPQPRRDPADR
HHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]