Click here to switch to the map view.

The map label for this gene is ntrB [H]

Identifier: 170748587

GI number: 170748587

Start: 2310823

End: 2311965

Strand: Reverse

Name: ntrB [H]

Synonym: Mrad2831_2169

Alternate gene names: 170748587

Gene position: 2311965-2310823 (Counterclockwise)

Preceding gene: 170748588

Following gene: 170748586

Centisome position: 38.04

GC content: 70.87

Gene sequence:

>1143_bases
GTGAGCGACGAGCCCCTCCCGAAAGAGTCCGGCAACCGGCGCGACACCGCCGCCGAGGCCGTGCTCAACGCCCTGCCGCT
GCCGGTACTCACCATCGGCGCCGACGAGCGGATCCTCCACTGCAACCACGCCGCGGAAGCGTTCTTCGACTATTCCGCCC
GGCTGATGCAGCGCCGTCGCCTGCGCGACATCATTCCCTTCGCCTCGCCGATCATCGCCCTGGTGGCCGAGGTGCGGCGG
CGCCAGGCCAGCGTCAGCGAATACCGGGTGGAGCTCACCCAGCCGCGCCTCGGCCTGGAGCGCAGCGTCGACGTGTTCGC
GACGCCGCTCGGCGACGACGGCGAGGCCGTGGTGATGATGCTCCAGGAGCGCACCATCGCCGACAAGATGAACCGCCAGC
TCACCCATCGCGGCGCGGCGCGCTCGATGGTGGCGCTCGGCGCGATGCTGGCCCACGAGATCAAGAACCCGCTCGCGGGC
ATCCGCGGCGCGGCGCAGCTCCTGGAGAGCACCGGTGCCGAGGAGGACCGGCTGCTCACCCGCCTGATCTGCGACGAATC
GGACCGGATCGTCCGCATCGTCGAGCGCATGGAGCTGTTCGGCGACGAGCGCCCCTCGGATCGCGGCGCCGTCAACGTCC
ACGGGGTGCTCGATCAGGTGAAGCGCTCGGCCCAGTCGGGCTTCGCGCGGCACATCCGGTTCATCGAGACCTACGACCCG
TCGCTGCCGCCGGTGCTGGGCAACCGCGACCAGCTGATCCAGGTGATCCTGAACCTCGTGAAGAACGCCGCCGAGGCGAT
CGGCCCGGACGCGGTCGAGGGCGAGATCACCCTGTCGACCGCCTTCCGCACCGGCCTGCGCCTGCAGGTCCCGGGCTCCC
GCGAGCGGGTCAGCCTGCCGATCGAGGTGGCGGTGCGCGACAACGGCCCGGGCATCTCGGCCGACCTGCTGCCCGACCTG
TTCGACCCGTTCGTCACCACCAAGGCGCAGGGCTCCGGCCTCGGTCTTGCATTGGTGGCCAAGATCGTCGGCGATCACGG
CGGGATCGTCGAGTGCGATCCCGCCCCGCGCCGCACGGCGTTCCGCATCCTTCTGCCGATGTCGAGTGCCCGGGACGGGC
GCGAATCGGCGGTCGATCCGTGA

Upstream 100 bases:

>100_bases
CCGAACGGTCGGCCCTCCTGACCACCACCGATCCGGAGACGGCGCTCGCCTTGCTGGCCCGCGCCTTCGCGGATGCGGCC
GATCCCGTCCAGGAGGCCGC

Downstream 100 bases:

>100_bases
TGTCGATCCCCGAATCCGGCCCCCGTCGGAGGTCTGAGTAGAGAGTCATGCCGAACGGCCACATCATCGTCGCCGACGAC
GACGCCGCCATCCGCACCGT

Product: nitrogen-specific signal transduction histidine kinase NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSDEPLPKESGNRRDTAAEAVLNALPLPVLTIGADERILHCNHAAEAFFDYSARLMQRRRLRDIIPFASPIIALVAEVRR
RQASVSEYRVELTQPRLGLERSVDVFATPLGDDGEAVVMMLQERTIADKMNRQLTHRGAARSMVALGAMLAHEIKNPLAG
IRGAAQLLESTGAEEDRLLTRLICDESDRIVRIVERMELFGDERPSDRGAVNVHGVLDQVKRSAQSGFARHIRFIETYDP
SLPPVLGNRDQLIQVILNLVKNAAEAIGPDAVEGEITLSTAFRTGLRLQVPGSRERVSLPIEVAVRDNGPGISADLLPDL
FDPFVTTKAQGSGLGLALVAKIVGDHGGIVECDPAPRRTAFRILLPMSSARDGRESAVDP

Sequences:

>Translated_380_residues
MSDEPLPKESGNRRDTAAEAVLNALPLPVLTIGADERILHCNHAAEAFFDYSARLMQRRRLRDIIPFASPIIALVAEVRR
RQASVSEYRVELTQPRLGLERSVDVFATPLGDDGEAVVMMLQERTIADKMNRQLTHRGAARSMVALGAMLAHEIKNPLAG
IRGAAQLLESTGAEEDRLLTRLICDESDRIVRIVERMELFGDERPSDRGAVNVHGVLDQVKRSAQSGFARHIRFIETYDP
SLPPVLGNRDQLIQVILNLVKNAAEAIGPDAVEGEITLSTAFRTGLRLQVPGSRERVSLPIEVAVRDNGPGISADLLPDL
FDPFVTTKAQGSGLGLALVAKIVGDHGGIVECDPAPRRTAFRILLPMSSARDGRESAVDP
>Mature_379_residues
SDEPLPKESGNRRDTAAEAVLNALPLPVLTIGADERILHCNHAAEAFFDYSARLMQRRRLRDIIPFASPIIALVAEVRRR
QASVSEYRVELTQPRLGLERSVDVFATPLGDDGEAVVMMLQERTIADKMNRQLTHRGAARSMVALGAMLAHEIKNPLAGI
RGAAQLLESTGAEEDRLLTRLICDESDRIVRIVERMELFGDERPSDRGAVNVHGVLDQVKRSAQSGFARHIRFIETYDPS
LPPVLGNRDQLIQVILNLVKNAAEAIGPDAVEGEITLSTAFRTGLRLQVPGSRERVSLPIEVAVRDNGPGISADLLPDLF
DPFVTTKAQGSGLGLALVAKIVGDHGGIVECDPAPRRTAFRILLPMSSARDGRESAVDP

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=357, Percent_Identity=34.453781512605, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1790436, Length=255, Percent_Identity=32.5490196078431, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788549, Length=251, Percent_Identity=31.0756972111554, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790567, Length=135, Percent_Identity=34.0740740740741, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41457; Mature: 41326

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDEPLPKESGNRRDTAAEAVLNALPLPVLTIGADERILHCNHAAEAFFDYSARLMQRRR
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCEEECCHHHHHHHHHHHHHHHHHH
LRDIIPFASPIIALVAEVRRRQASVSEYRVELTQPRLGLERSVDVFATPLGDDGEAVVMM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHEEHH
LQERTIADKMNRQLTHRGAARSMVALGAMLAHEIKNPLAGIRGAAQLLESTGAEEDRLLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RLICDESDRIVRIVERMELFGDERPSDRGAVNVHGVLDQVKRSAQSGFARHIRFIETYDP
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLPPVLGNRDQLIQVILNLVKNAAEAIGPDAVEGEITLSTAFRTGLRLQVPGSRERVSLP
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHCCCEEECCCCCCEEECE
IEVAVRDNGPGISADLLPDLFDPFVTTKAQGSGLGLALVAKIVGDHGGIVECDPAPRRTA
EEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCE
FRILLPMSSARDGRESAVDP
EEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SDEPLPKESGNRRDTAAEAVLNALPLPVLTIGADERILHCNHAAEAFFDYSARLMQRRR
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCEEECCHHHHHHHHHHHHHHHHHH
LRDIIPFASPIIALVAEVRRRQASVSEYRVELTQPRLGLERSVDVFATPLGDDGEAVVMM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHEEHH
LQERTIADKMNRQLTHRGAARSMVALGAMLAHEIKNPLAGIRGAAQLLESTGAEEDRLLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RLICDESDRIVRIVERMELFGDERPSDRGAVNVHGVLDQVKRSAQSGFARHIRFIETYDP
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLPPVLGNRDQLIQVILNLVKNAAEAIGPDAVEGEITLSTAFRTGLRLQVPGSRERVSLP
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHCCCEEECCCCCCEEECE
IEVAVRDNGPGISADLLPDLFDPFVTTKAQGSGLGLALVAKIVGDHGGIVECDPAPRRTA
EEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCE
FRILLPMSSARDGRESAVDP
EEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3020561 [H]