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The map label for this gene is ntrC [H]

Identifier: 170748586

GI number: 170748586

Start: 2309309

End: 2310775

Strand: Reverse

Name: ntrC [H]

Synonym: Mrad2831_2168

Alternate gene names: 170748586

Gene position: 2310775-2309309 (Counterclockwise)

Preceding gene: 170748587

Following gene: 170748584

Centisome position: 38.02

GC content: 71.78

Gene sequence:

>1467_bases
ATGCCGAACGGCCACATCATCGTCGCCGACGACGACGCCGCCATCCGCACCGTGCTGAACCAAGCGCTGTCCCGGGCGGG
CTACGAGGTCCGCTCGACGGGCAATTGCGCCACCCTCTGGCGCTGGGTCGCGCAGGGGGAGGGCGACCTCGTCATCACCG
ACGTGGTCATGCCCGACGAGAACGTGTTCGACCTGCTGCCGCGCATCAAGCGGGTGCGGCCGGAGCTGCCGATCATCGTG
ATGAGCGCGCAGAACACCTTCATGACGGCGATCCGCGCCTCGGAGCGGGGCGCCTACGAGTACCTGCCGAAGCCCTTCGA
CCTGAAGGAGCTGATCGCCATCGTCGGCCGGGCGCTCTCGCGCCCCCGGGGCGCGGCGCCGGCCGGGGCGCCCCCCGAGA
ACGAGGACATCCCGCTGGTCGGCCGCTCGCCGGCCATGCAGGAGATCTACCGGGCGCTCGCCCGGCTGATGCCCACCGAC
CTCACCGTGATGATCACCGGCGAGTCCGGCACCGGCAAGGAGCTGGTCGCCCGGGCGCTGCACGATTACGGCCGCCGCCG
CACCGGCCCGTTCGTGCCGGTCAATATGGCGGCGATCCCGCGCGACCTGATCGAGTCCGAGCTGTTCGGCCACGAGAAGG
GCGCCTTCACGGGCGCGCTCCAGCGCTCCGCCGGCCGGTTCGAGCAGGCCGAGGGCGGCACGCTGTTCCTCGACGAGATC
GGCGACATGCCCATGGAGGCGCAGACCCGGCTGCTGCGCGTGCTCCAGCAGGGCGAGTACACGACGGTGGGCGGCCGCGT
CCCGATCCGCACCAACGTCCGGATCATCGCGGCCACCAACAAGGACCTGCGGGTCTCCATCCAGCAGGGCATCTTCCGGG
AGGACCTGTTCTTCCGCCTCAACGTCGTGCCGCTGCGGCTGCCGGCCCTGCGCGAGCGCTCCGAGGACGTGCCGGACCTC
GTCCGCCACTTCTTCGTGCTCGTCGAGCGCGAGGGCCTGACCCGGAAGACCCTCGACGGCGACGCCATGGAGCGGCTGAA
GCGCTACCGCTGGCCCGGCAACGTCCGCGAGCTCGAGAACCTCGTCCGGCGGCTCGCCGCCCTCTACCCGCAGGAGACGA
TCACCGGCCCGGTGATCGAGGCCGAGCTCGACACGCTGCCGCTGGCGACGCCCGCGGCGCCGGCGGCCAACGGCGCCGCC
CGCAAGGCGAACGGGGAATCGGAGACCCTGTCCAGCGCCGTGGAGCGCCACCTCTCGGACTACTTCTCCGGCTATCGCGA
CACGCTGCCGCCCCCCGGCCTGTATCACCGGATCCTGCGGGAGATCGAGGGCCCGCTGATCGGCGCGGCGCTCGCCGCCA
CCCGCGGCAACCAGATCCGCGCCGCGGAACTGCTCGGCGTCAACCGCAACACCCTGCGCAAGAAGGTCCGCGACCTCGAC
CTGCAGGTCTTCCGGACGGCGCGGTAG

Upstream 100 bases:

>100_bases
TTCTGCCGATGTCGAGTGCCCGGGACGGGCGCGAATCGGCGGTCGATCCGTGATGTCGATCCCCGAATCCGGCCCCCGTC
GGAGGTCTGAGTAGAGAGTC

Downstream 100 bases:

>100_bases
GGCGGTCCCCGGCCGATCCGGCCGGGTCCCCTCAACCCGAGGCGCGTCGGGCGCCGCGCGACGAGGTCCAGAACGGGATG
AGCGCGGTCCTGGCCGCCCT

Product: Fis family nitrogen metabolism transcriptional regulator NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MPNGHIIVADDDAAIRTVLNQALSRAGYEVRSTGNCATLWRWVAQGEGDLVITDVVMPDENVFDLLPRIKRVRPELPIIV
MSAQNTFMTAIRASERGAYEYLPKPFDLKELIAIVGRALSRPRGAAPAGAPPENEDIPLVGRSPAMQEIYRALARLMPTD
LTVMITGESGTGKELVARALHDYGRRRTGPFVPVNMAAIPRDLIESELFGHEKGAFTGALQRSAGRFEQAEGGTLFLDEI
GDMPMEAQTRLLRVLQQGEYTTVGGRVPIRTNVRIIAATNKDLRVSIQQGIFREDLFFRLNVVPLRLPALRERSEDVPDL
VRHFFVLVEREGLTRKTLDGDAMERLKRYRWPGNVRELENLVRRLAALYPQETITGPVIEAELDTLPLATPAAPAANGAA
RKANGESETLSSAVERHLSDYFSGYRDTLPPPGLYHRILREIEGPLIGAALAATRGNQIRAAELLGVNRNTLRKKVRDLD
LQVFRTAR

Sequences:

>Translated_488_residues
MPNGHIIVADDDAAIRTVLNQALSRAGYEVRSTGNCATLWRWVAQGEGDLVITDVVMPDENVFDLLPRIKRVRPELPIIV
MSAQNTFMTAIRASERGAYEYLPKPFDLKELIAIVGRALSRPRGAAPAGAPPENEDIPLVGRSPAMQEIYRALARLMPTD
LTVMITGESGTGKELVARALHDYGRRRTGPFVPVNMAAIPRDLIESELFGHEKGAFTGALQRSAGRFEQAEGGTLFLDEI
GDMPMEAQTRLLRVLQQGEYTTVGGRVPIRTNVRIIAATNKDLRVSIQQGIFREDLFFRLNVVPLRLPALRERSEDVPDL
VRHFFVLVEREGLTRKTLDGDAMERLKRYRWPGNVRELENLVRRLAALYPQETITGPVIEAELDTLPLATPAAPAANGAA
RKANGESETLSSAVERHLSDYFSGYRDTLPPPGLYHRILREIEGPLIGAALAATRGNQIRAAELLGVNRNTLRKKVRDLD
LQVFRTAR
>Mature_487_residues
PNGHIIVADDDAAIRTVLNQALSRAGYEVRSTGNCATLWRWVAQGEGDLVITDVVMPDENVFDLLPRIKRVRPELPIIVM
SAQNTFMTAIRASERGAYEYLPKPFDLKELIAIVGRALSRPRGAAPAGAPPENEDIPLVGRSPAMQEIYRALARLMPTDL
TVMITGESGTGKELVARALHDYGRRRTGPFVPVNMAAIPRDLIESELFGHEKGAFTGALQRSAGRFEQAEGGTLFLDEIG
DMPMEAQTRLLRVLQQGEYTTVGGRVPIRTNVRIIAATNKDLRVSIQQGIFREDLFFRLNVVPLRLPALRERSEDVPDLV
RHFFVLVEREGLTRKTLDGDAMERLKRYRWPGNVRELENLVRRLAALYPQETITGPVIEAELDTLPLATPAAPAANGAAR
KANGESETLSSAVERHLSDYFSGYRDTLPPPGLYHRILREIEGPLIGAALAATRGNQIRAAELLGVNRNTLRKKVRDLDL
QVFRTAR

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=489, Percent_Identity=43.3537832310838, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1790437, Length=475, Percent_Identity=40, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1788550, Length=483, Percent_Identity=38.3022774327122, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1788905, Length=389, Percent_Identity=40.1028277634961, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1789233, Length=356, Percent_Identity=40.7303370786517, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI87082117, Length=233, Percent_Identity=51.0729613733906, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1789087, Length=342, Percent_Identity=40.3508771929825, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87082152, Length=332, Percent_Identity=40.3614457831325, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI87081872, Length=342, Percent_Identity=39.766081871345, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1786524, Length=249, Percent_Identity=45.7831325301205, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1787583, Length=227, Percent_Identity=41.4096916299559, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1789828, Length=241, Percent_Identity=35.6846473029046, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI87081858, Length=209, Percent_Identity=34.4497607655502, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789809, Length=140, Percent_Identity=31.4285714285714, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1786784, Length=230, Percent_Identity=26.9565217391304, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54128; Mature: 53997

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNGHIIVADDDAAIRTVLNQALSRAGYEVRSTGNCATLWRWVAQGEGDLVITDVVMPDE
CCCCCEEEECCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCEEEEEEECCCC
NVFDLLPRIKRVRPELPIIVMSAQNTFMTAIRASERGAYEYLPKPFDLKELIAIVGRALS
HHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHC
RPRGAAPAGAPPENEDIPLVGRSPAMQEIYRALARLMPTDLTVMITGESGTGKELVARAL
CCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHH
HDYGRRRTGPFVPVNMAAIPRDLIESELFGHEKGAFTGALQRSAGRFEQAEGGTLFLDEI
HHHHCCCCCCCCEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEHHHH
GDMPMEAQTRLLRVLQQGEYTTVGGRVPIRTNVRIIAATNKDLRVSIQQGIFREDLFFRL
CCCCHHHHHHHHHHHHCCCEEEECCEEEEECCEEEEEECCCCEEEEHHHCCHHHHHEEEE
NVVPLRLPALRERSEDVPDLVRHFFVLVEREGLTRKTLDGDAMERLKRYRWPGNVRELEN
EEEEECCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
LVRRLAALYPQETITGPVIEAELDTLPLATPAAPAANGAARKANGESETLSSAVERHLSD
HHHHHHHHCCCHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
YFSGYRDTLPPPGLYHRILREIEGPLIGAALAATRGNQIRAAELLGVNRNTLRKKVRDLD
HHCCHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHHH
LQVFRTAR
HHHHHCCC
>Mature Secondary Structure 
PNGHIIVADDDAAIRTVLNQALSRAGYEVRSTGNCATLWRWVAQGEGDLVITDVVMPDE
CCCCEEEECCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCEEEEEEECCCC
NVFDLLPRIKRVRPELPIIVMSAQNTFMTAIRASERGAYEYLPKPFDLKELIAIVGRALS
HHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHC
RPRGAAPAGAPPENEDIPLVGRSPAMQEIYRALARLMPTDLTVMITGESGTGKELVARAL
CCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHH
HDYGRRRTGPFVPVNMAAIPRDLIESELFGHEKGAFTGALQRSAGRFEQAEGGTLFLDEI
HHHHCCCCCCCCEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEHHHH
GDMPMEAQTRLLRVLQQGEYTTVGGRVPIRTNVRIIAATNKDLRVSIQQGIFREDLFFRL
CCCCHHHHHHHHHHHHCCCEEEECCEEEEECCEEEEEECCCCEEEEHHHCCHHHHHEEEE
NVVPLRLPALRERSEDVPDLVRHFFVLVEREGLTRKTLDGDAMERLKRYRWPGNVRELEN
EEEEECCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
LVRRLAALYPQETITGPVIEAELDTLPLATPAAPAANGAARKANGESETLSSAVERHLSD
HHHHHHHHCCCHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
YFSGYRDTLPPPGLYHRILREIEGPLIGAALAATRGNQIRAAELLGVNRNTLRKKVRDLD
HHCCHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHHH
LQVFRTAR
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]