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The map label for this gene is metF [H]

Identifier: 170748542

GI number: 170748542

Start: 2264315

End: 2265232

Strand: Reverse

Name: metF [H]

Synonym: Mrad2831_2124

Alternate gene names: 170748542

Gene position: 2265232-2264315 (Counterclockwise)

Preceding gene: 170748543

Following gene: 170748541

Centisome position: 37.27

GC content: 69.39

Gene sequence:

>918_bases
ATGCGCCGCTCCACCCACCGCGCCAGCCGCGACGGCTACCACCCGATCCGCGTCTCGATCGAGTTCTTCCCGCCGAAGAC
CGACGAGATGGAGAAGATCCTCTGGAACTCCATCGAGCGCCTCGCGCCGCTGCAGCCGAGCTTCGTCTCGGTGACCTACG
GGGCCGGTGGCTCGACCCGCGAGCGCACTCACAACACGGTCGCCCGGATGGTCCGCGAGACCGGCCTCAAGCCCGCCGCC
CACCTCACCTGCGTGGACGCCTCCCGCGACGAGATCGACGCGGTGGTGCAGTCCTACTGGGACGCGGGCGTGCGCCACAT
CGTGGCCCTGCGCGGCGACCCGGCCGAGGGCGTCGGCCACGCCTACCGGCCCCATCCGGAAGGCTACGCCTCGACCGCCG
AGCTGGTGCAGGGGATCAAGCGGATCGGCGACTTCAAGGTCTCGGTCTCGGCCTACCCCGAGAAGCATCCCGAGGCGGCC
TCGCTGGACGCCGACATCGACGCCCTGAAGGCCAAAGTCGATGCCGGGGCCGATCAGGCGATCACCCAGTTCTTCTTCGA
GAACGAGATCTACCTGCGCTACCTGGAGAAGGTCCGGGCGGCCGGGATCACCATCCCGATCATCCCGGGCATCCTGCCGG
TGCAGAACTTCAAGCAGGCGGCGAACTTCGCCCGCCGCACCGGCGCCTCGGTGCCCTACTGGCTGGCCGCCCGGTTCGAG
GGGCTGGAGGACGACGTGGAGACCCGCCGCCTCGTCGCCGCCGCGGTGGCGGCTGAGCAGGTGCTCGACTTGGTGGATGA
GGGCGTCAACGACTTCCACTTCTACTCGATGAACCGCTCGGATCTGGTCTACGCGATCTGCCACCTGCTCGGCCTGCGCG
CTCAGGCGCCGAAGCTGGCGGCGAAGGCGGCGGCTTGA

Upstream 100 bases:

>100_bases
ACGGGCTGACCGACACAGAACAGCCTCAGCGCGCCGTCGCCTGAGACACCGAACTCCGCAGACTTCGTCTGAACCGACCG
ACATAGAGGACGAGACCCCG

Downstream 100 bases:

>100_bases
TTCTCGAGACTGCGCTCGACGACCCCGATCCGCCATCGCGCGCGCAGCGAAGCACCCAGGGCAGCGCGACCTCCGTGAGC
GTGGCGCAGCTGGATCGCTT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MRRSTHRASRDGYHPIRVSIEFFPPKTDEMEKILWNSIERLAPLQPSFVSVTYGAGGSTRERTHNTVARMVRETGLKPAA
HLTCVDASRDEIDAVVQSYWDAGVRHIVALRGDPAEGVGHAYRPHPEGYASTAELVQGIKRIGDFKVSVSAYPEKHPEAA
SLDADIDALKAKVDAGADQAITQFFFENEIYLRYLEKVRAAGITIPIIPGILPVQNFKQAANFARRTGASVPYWLAARFE
GLEDDVETRRLVAAAVAAEQVLDLVDEGVNDFHFYSMNRSDLVYAICHLLGLRAQAPKLAAKAAA

Sequences:

>Translated_305_residues
MRRSTHRASRDGYHPIRVSIEFFPPKTDEMEKILWNSIERLAPLQPSFVSVTYGAGGSTRERTHNTVARMVRETGLKPAA
HLTCVDASRDEIDAVVQSYWDAGVRHIVALRGDPAEGVGHAYRPHPEGYASTAELVQGIKRIGDFKVSVSAYPEKHPEAA
SLDADIDALKAKVDAGADQAITQFFFENEIYLRYLEKVRAAGITIPIIPGILPVQNFKQAANFARRTGASVPYWLAARFE
GLEDDVETRRLVAAAVAAEQVLDLVDEGVNDFHFYSMNRSDLVYAICHLLGLRAQAPKLAAKAAA
>Mature_305_residues
MRRSTHRASRDGYHPIRVSIEFFPPKTDEMEKILWNSIERLAPLQPSFVSVTYGAGGSTRERTHNTVARMVRETGLKPAA
HLTCVDASRDEIDAVVQSYWDAGVRHIVALRGDPAEGVGHAYRPHPEGYASTAELVQGIKRIGDFKVSVSAYPEKHPEAA
SLDADIDALKAKVDAGADQAITQFFFENEIYLRYLEKVRAAGITIPIIPGILPVQNFKQAANFARRTGASVPYWLAARFE
GLEDDVETRRLVAAAVAAEQVLDLVDEGVNDFHFYSMNRSDLVYAICHLLGLRAQAPKLAAKAAA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=287, Percent_Identity=33.1010452961672, Blast_Score=158, Evalue=7e-39,
Organism=Escherichia coli, GI1790377, Length=278, Percent_Identity=55.7553956834532, Blast_Score=322, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI25147436, Length=286, Percent_Identity=29.7202797202797, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25147441, Length=286, Percent_Identity=29.7202797202797, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321313, Length=269, Percent_Identity=32.7137546468402, Blast_Score=142, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6325234, Length=315, Percent_Identity=33.3333333333333, Blast_Score=142, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24662658, Length=228, Percent_Identity=28.0701754385965, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33612; Mature: 33612

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSTHRASRDGYHPIRVSIEFFPPKTDEMEKILWNSIERLAPLQPSFVSVTYGAGGSTR
CCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCH
ERTHNTVARMVRETGLKPAAHLTCVDASRDEIDAVVQSYWDAGVRHIVALRGDPAEGVGH
HHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCC
AYRPHPEGYASTAELVQGIKRIGDFKVSVSAYPEKHPEAASLDADIDALKAKVDAGADQA
CCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCHHHH
ITQFFFENEIYLRYLEKVRAAGITIPIIPGILPVQNFKQAANFARRTGASVPYWLAARFE
HHHHHHCCHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHC
GLEDDVETRRLVAAAVAAEQVLDLVDEGVNDFHFYSMNRSDLVYAICHLLGLRAQAPKLA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHH
AKAAA
HHCCC
>Mature Secondary Structure
MRRSTHRASRDGYHPIRVSIEFFPPKTDEMEKILWNSIERLAPLQPSFVSVTYGAGGSTR
CCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCH
ERTHNTVARMVRETGLKPAAHLTCVDASRDEIDAVVQSYWDAGVRHIVALRGDPAEGVGH
HHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCC
AYRPHPEGYASTAELVQGIKRIGDFKVSVSAYPEKHPEAASLDADIDALKAKVDAGADQA
CCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCHHHH
ITQFFFENEIYLRYLEKVRAAGITIPIIPGILPVQNFKQAANFARRTGASVPYWLAARFE
HHHHHHCCHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHC
GLEDDVETRRLVAAAVAAEQVLDLVDEGVNDFHFYSMNRSDLVYAICHLLGLRAQAPKLA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHH
AKAAA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6356036; 8346018; 9278503; 10201405 [H]