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The map label for this gene is phoQ [H]

Identifier: 170748482

GI number: 170748482

Start: 2194267

End: 2195592

Strand: Reverse

Name: phoQ [H]

Synonym: Mrad2831_2064

Alternate gene names: 170748482

Gene position: 2195592-2194267 (Counterclockwise)

Preceding gene: 170748483

Following gene: 170748480

Centisome position: 36.12

GC content: 78.36

Gene sequence:

>1326_bases
ATGACGGGGAGGGCGCCGCGGCGCTCGCTCCGCACCCGGCTGCTCGCGGCGGCCCTGGTGCTGGTCCTGGCGGCCCTGGT
GGTGGCGGGCCTCGTCATAGGGGTGATACTGCACCGGTTCGTGCGCGGGCAGCTCGACGGCCGGCTCGACGCGCAGATCG
TCGCCCTGCGGGCCGGGCTCGAGGCCGGCATCCTCCCGCAGGGTCTCGACGCGCCGCCCTTCGACCGGCCCGGCCCCGGC
TGGGCCTGGGAGGTGCGCCGCGGCGGCGCGACGATCCGGTCCGGCTCCCTGCGGGGCGGGGACATCCGCCTGTCCGATCC
CGAACCGGACGGGGAGCCGGGCCGGCCGCACCCGGCTTCGGGCACGGGACCGCGCGGCGAGCCCCTCGTCGCCCGCGTGC
TGGACGTGCCCGGGCCCGAGCCGGCGACGATCGTGGTGGCGGCACCGGAGGGGGAGCTGCGCGGGCCGCTGCGCGAGGCG
CTGACGAGCCTCGCCCTGGCGCTCGGCATCCTCGGCCTGTGCCTGCTCGCCGGGCTGGCGGTCCAGGTGCGCCTGGGCCT
CGCGCCGCTGACGCGCCTGCGCGCGGACCTCGCCTCGGTCCGGTCGGGGCGGCGCGAGCGCGTGCCGGCGGACCAGCCCG
CCGAGATCGCGCCGGTGGTGGCGGAACTCAACGCGCTCCTCGACCAGAACGCGCAGAACCTGGAACGCGCCCGCGGCCAC
GTCGCCAACCTCGCCCACGCCCTCAAGACGCCGCTCGCCACCCTGTCGATGGCGCTCGCGGAGCCCGCCCGCGATCCGGA
CGGGGCGCTGCGCCGGCAGGTGGACGACATGGACCGGCGGGTGCGGCACCACCTCCGGCGCGCCCGGGCCGCGGCCCTGG
CGGGTTCGGCCCGGGGCCGCACGGCCCTGGCGCCCCGGCTGTCGGACTTGCGCGCGGCGCTGGCCCGGATCCACGCCGAG
AAGGGCGCGGCGATCGACCTCGACGTCCCGGAGGCCCTGGCGGTCTCCTGCGAGGGCCAGGATCTCGACGAGATGCTCGG
CAACCTCGTGGACAATGCCTGCCGCTGGTGCCGCGGCCGCGTGCGGGTCTCCGCGACGGCGCGCGATGGCGCGGTGGCCG
TGTCGGTGGAGGATGACGGGCCCGGCCTCGACCCGGCGGCCGCCTCCGCCGTCATGGCCCGGGGCCGGCGCCTCGACGAG
GGCGTGCCGGGCCACGGCTTCGGGCTGCCGATCACCCTGGAACTCGCCGAGCTGTACGGCGGCGGCCTGAGCCTCGGGCG
CTCGGATCTCGGCGGCCTCCGCGCGGACTTGGTGCTGCCGGCCTGA

Upstream 100 bases:

>100_bases
CCGTCGAGGTGATCATCACGCGCCTGCGCCGGAAGATCGCCCCGGCGCGGATCGAGAGCGTGCGCGGCCACGGCTACCGC
CTGCACGCGGAGCCCGCCTG

Downstream 100 bases:

>100_bases
CCGGCCCCCCTCAGCCGAGCGCCTCCCGCAGGATCGGCCGGATCCGCGGATCGGCGCAGTGGGCGACGTTGAAACGCATG
TAGCCGTTCCACGTATCCGA

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTGRAPRRSLRTRLLAAALVLVLAALVVAGLVIGVILHRFVRGQLDGRLDAQIVALRAGLEAGILPQGLDAPPFDRPGPG
WAWEVRRGGATIRSGSLRGGDIRLSDPEPDGEPGRPHPASGTGPRGEPLVARVLDVPGPEPATIVVAAPEGELRGPLREA
LTSLALALGILGLCLLAGLAVQVRLGLAPLTRLRADLASVRSGRRERVPADQPAEIAPVVAELNALLDQNAQNLERARGH
VANLAHALKTPLATLSMALAEPARDPDGALRRQVDDMDRRVRHHLRRARAAALAGSARGRTALAPRLSDLRAALARIHAE
KGAAIDLDVPEALAVSCEGQDLDEMLGNLVDNACRWCRGRVRVSATARDGAVAVSVEDDGPGLDPAAASAVMARGRRLDE
GVPGHGFGLPITLELAELYGGGLSLGRSDLGGLRADLVLPA

Sequences:

>Translated_441_residues
MTGRAPRRSLRTRLLAAALVLVLAALVVAGLVIGVILHRFVRGQLDGRLDAQIVALRAGLEAGILPQGLDAPPFDRPGPG
WAWEVRRGGATIRSGSLRGGDIRLSDPEPDGEPGRPHPASGTGPRGEPLVARVLDVPGPEPATIVVAAPEGELRGPLREA
LTSLALALGILGLCLLAGLAVQVRLGLAPLTRLRADLASVRSGRRERVPADQPAEIAPVVAELNALLDQNAQNLERARGH
VANLAHALKTPLATLSMALAEPARDPDGALRRQVDDMDRRVRHHLRRARAAALAGSARGRTALAPRLSDLRAALARIHAE
KGAAIDLDVPEALAVSCEGQDLDEMLGNLVDNACRWCRGRVRVSATARDGAVAVSVEDDGPGLDPAAASAVMARGRRLDE
GVPGHGFGLPITLELAELYGGGLSLGRSDLGGLRADLVLPA
>Mature_440_residues
TGRAPRRSLRTRLLAAALVLVLAALVVAGLVIGVILHRFVRGQLDGRLDAQIVALRAGLEAGILPQGLDAPPFDRPGPGW
AWEVRRGGATIRSGSLRGGDIRLSDPEPDGEPGRPHPASGTGPRGEPLVARVLDVPGPEPATIVVAAPEGELRGPLREAL
TSLALALGILGLCLLAGLAVQVRLGLAPLTRLRADLASVRSGRRERVPADQPAEIAPVVAELNALLDQNAQNLERARGHV
ANLAHALKTPLATLSMALAEPARDPDGALRRQVDDMDRRVRHHLRRARAAALAGSARGRTALAPRLSDLRAALARIHAEK
GAAIDLDVPEALAVSCEGQDLDEMLGNLVDNACRWCRGRVRVSATARDGAVAVSVEDDGPGLDPAAASAVMARGRRLDEG
VPGHGFGLPITLELAELYGGGLSLGRSDLGGLRADLVLPA

Specific function: Member of the two-component regulatory system phoQ/phoP involved in virulence, adaptation to low Mg(2+) environments and the control of acid resistance genes. In presence of low periplasmic Mg(2+) concentrations, phoQ functions as a membrane- associated p

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787374, Length=307, Percent_Identity=28.6644951140065, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788393, Length=276, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790551, Length=295, Percent_Identity=29.1525423728814, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789808, Length=208, Percent_Identity=27.4038461538462, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR015014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF08918 PhoQ_Sensor [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 46022; Mature: 45890

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRAPRRSLRTRLLAAALVLVLAALVVAGLVIGVILHRFVRGQLDGRLDAQIVALRAGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
EAGILPQGLDAPPFDRPGPGWAWEVRRGGATIRSGSLRGGDIRLSDPEPDGEPGRPHPAS
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCCEEECCCCCCCCCCCCCCCC
GTGPRGEPLVARVLDVPGPEPATIVVAAPEGELRGPLREALTSLALALGILGLCLLAGLA
CCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VQVRLGLAPLTRLRADLASVRSGRRERVPADQPAEIAPVVAELNALLDQNAQNLERARGH
HHHHHCHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
VANLAHALKTPLATLSMALAEPARDPDGALRRQVDDMDRRVRHHLRRARAAALAGSARGR
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TALAPRLSDLRAALARIHAEKGAAIDLDVPEALAVSCEGQDLDEMLGNLVDNACRWCRGR
CHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHEEECCCCCHHHHHHHHHHHHHHHHCCC
VRVSATARDGAVAVSVEDDGPGLDPAAASAVMARGRRLDEGVPGHGFGLPITLELAELYG
EEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHCC
GGLSLGRSDLGGLRADLVLPA
CCHHCCCHHHCCCCCCEECCC
>Mature Secondary Structure 
TGRAPRRSLRTRLLAAALVLVLAALVVAGLVIGVILHRFVRGQLDGRLDAQIVALRAGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
EAGILPQGLDAPPFDRPGPGWAWEVRRGGATIRSGSLRGGDIRLSDPEPDGEPGRPHPAS
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCCEEECCCCCCCCCCCCCCCC
GTGPRGEPLVARVLDVPGPEPATIVVAAPEGELRGPLREALTSLALALGILGLCLLAGLA
CCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VQVRLGLAPLTRLRADLASVRSGRRERVPADQPAEIAPVVAELNALLDQNAQNLERARGH
HHHHHCHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
VANLAHALKTPLATLSMALAEPARDPDGALRRQVDDMDRRVRHHLRRARAAALAGSARGR
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TALAPRLSDLRAALARIHAEKGAAIDLDVPEALAVSCEGQDLDEMLGNLVDNACRWCRGR
CHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHEEECCCCCHHHHHHHHHHHHHHHHCCC
VRVSATARDGAVAVSVEDDGPGLDPAAASAVMARGRRLDEGVPGHGFGLPITLELAELYG
EEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHCC
GGLSLGRSDLGGLRADLVLPA
CCHHCCCHHHCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]