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The map label for this gene is nuoM [H]

Identifier: 170748465

GI number: 170748465

Start: 2176665

End: 2178185

Strand: Reverse

Name: nuoM [H]

Synonym: Mrad2831_2047

Alternate gene names: 170748465

Gene position: 2178185-2176665 (Counterclockwise)

Preceding gene: 170748466

Following gene: 170748464

Centisome position: 35.84

GC content: 65.61

Gene sequence:

>1521_bases
ATGCTCGGCCTCGGCATCCTCTCCGGCCTGCTGATCGTGCCGCTCTGCGGCGCGGCCTTCATCCTGACGCTGAACGGCGA
CGAGGAATCGGTCGCGCGCAACAGCCGCTGGGCCGCGCTCGCCACCACGGTGGTCACCTTCCTGCTCTCCCTGGTGGCCT
GGAGCCGCTACGACGCCGCCTCGTCGAGCTTCCAGCTGGTGGAGAGCCACGCCTGGCTCACCGAGACGATCCGGTTCAAG
CTCGGCGTCGACGGCTTCTCGATGCCGCTGATCCTGCTGACCACGTTCCTGATGCCGTTCTGCATCGGCGCCTCGTGGCA
CTCGATCCACTTCCGGGTGAAGGAGTACTTCGTCGCCTTCCTCGTCCTCGAGACGACGATGATCGGCGTGTTCGCCTCCC
TCGACCTGCTGCTGTTCTACCTGTTCTTCGAGGCCGGCCTGATCCCGATGTTCCTGATCATCGGCATCTGGGGCGGGCAG
CGCCGGATCTACGCGAGCTTCAAGTTCTTCCTCTACACCCTGCTCGGCTCGGTGCTGATGCTGCTCGCCGTGATGGCGAT
GTACTGGCAGGCCGGAACCACCGACATCCCGACCCTGCTGCAGTACCGCTTCCCGGTGCAGATGCAGACCTGGCTGTGGC
TCGCCTTCTTCGCCTCGTTCGCGGTGAAGATGCCGATGTGGCCGGTCCACACCTGGCTGCCCGACGCCCACGTGGAGGCC
CCCACCGCTGGCTCGGTGATCCTGGCCGGCATCCTCCTGAAGATGGGCGGCTACGGCTTCATCCGGATCTCGCTGCCGAT
GCTGCCGGTGGCGAGCCAGGAGTTCGCGCCCTTCGTCTTCGCCCTCTCGGTGATCGCGATCATCTTCACCTCGCTCACCG
CGATGATGCAGACCGACATCAAGAAGCTGATCGCCTACTCGTCGGTGGCCCATATGGGCTTCGTGACGCTCGGCCTGTTC
ACCTTGAACGAGCAGGGCATCCAGGGCGCGCTGTTCCTGATGATCTCCCACGGCATCGTGTCGGGGGCGCTCTTCCTCTG
CGTCGGCGTCGTCTACGACCGGATGCACACCCGCGAGATCGCCGCCTATGGCGGCCTCGTGAAGCGGATGCCGCTCTACG
CCGTGGCCATGCTGATCTTCACCATGGCCAATGTCGGCCTCCCCGGCACCTCCGGCTTCGTCGGCGAGTTCCTGGCGATG
CTCGGCGCCTTCAAGGCGAACCCGAACGTCGCGTTCTTCTCGACCTTCGGCATCATCCTGTCGGCGGCCTACGCCCTGTG
GCTCTACGCCCGGGTGATCTACGGCAAGCTGGAGAAGCCGAACCTCCAGGGCATCCTGGATCTCGACACCCGTGAAAAGA
TCATCCTGGCGCCGCTGGTGGTGCTGACGATCTACTACGGCGTGCATCCGGCCCCGGTGCTCGACGTGTTCGCGCCCTCG
ACCGACGCGCTCATGGCGAGCATCAAGACCGCGCTCTCGAACACCCAGACGGCCGCCGCGGCGCTGCCGATCCCGCGCTG
A

Upstream 100 bases:

>100_bases
ACCGGCTACCTGTACCATTACGCCTTCGTGATGCTCATCGGCGTCGCCGGCCTGATCAGCTGGTACCTGATGTCCGGCCT
GCCCAAGGGTGCTCACTGAT

Downstream 100 bases:

>100_bases
GGCGGACACAATGACAACCGTCCACTCCGTCCTGCCGTCGCTGGCGCCGCTCCTGCCCGAGCTGATCCTCGGCGTCGGCG
TCATGGTCCTGATCCTCTAC

Product: proton-translocating NADH-quinone oxidoreductase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MLGLGILSGLLIVPLCGAAFILTLNGDEESVARNSRWAALATTVVTFLLSLVAWSRYDAASSSFQLVESHAWLTETIRFK
LGVDGFSMPLILLTTFLMPFCIGASWHSIHFRVKEYFVAFLVLETTMIGVFASLDLLLFYLFFEAGLIPMFLIIGIWGGQ
RRIYASFKFFLYTLLGSVLMLLAVMAMYWQAGTTDIPTLLQYRFPVQMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVEA
PTAGSVILAGILLKMGGYGFIRISLPMLPVASQEFAPFVFALSVIAIIFTSLTAMMQTDIKKLIAYSSVAHMGFVTLGLF
TLNEQGIQGALFLMISHGIVSGALFLCVGVVYDRMHTREIAAYGGLVKRMPLYAVAMLIFTMANVGLPGTSGFVGEFLAM
LGAFKANPNVAFFSTFGIILSAAYALWLYARVIYGKLEKPNLQGILDLDTREKIILAPLVVLTIYYGVHPAPVLDVFAPS
TDALMASIKTALSNTQTAAAALPIPR

Sequences:

>Translated_506_residues
MLGLGILSGLLIVPLCGAAFILTLNGDEESVARNSRWAALATTVVTFLLSLVAWSRYDAASSSFQLVESHAWLTETIRFK
LGVDGFSMPLILLTTFLMPFCIGASWHSIHFRVKEYFVAFLVLETTMIGVFASLDLLLFYLFFEAGLIPMFLIIGIWGGQ
RRIYASFKFFLYTLLGSVLMLLAVMAMYWQAGTTDIPTLLQYRFPVQMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVEA
PTAGSVILAGILLKMGGYGFIRISLPMLPVASQEFAPFVFALSVIAIIFTSLTAMMQTDIKKLIAYSSVAHMGFVTLGLF
TLNEQGIQGALFLMISHGIVSGALFLCVGVVYDRMHTREIAAYGGLVKRMPLYAVAMLIFTMANVGLPGTSGFVGEFLAM
LGAFKANPNVAFFSTFGIILSAAYALWLYARVIYGKLEKPNLQGILDLDTREKIILAPLVVLTIYYGVHPAPVLDVFAPS
TDALMASIKTALSNTQTAAAALPIPR
>Mature_506_residues
MLGLGILSGLLIVPLCGAAFILTLNGDEESVARNSRWAALATTVVTFLLSLVAWSRYDAASSSFQLVESHAWLTETIRFK
LGVDGFSMPLILLTTFLMPFCIGASWHSIHFRVKEYFVAFLVLETTMIGVFASLDLLLFYLFFEAGLIPMFLIIGIWGGQ
RRIYASFKFFLYTLLGSVLMLLAVMAMYWQAGTTDIPTLLQYRFPVQMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVEA
PTAGSVILAGILLKMGGYGFIRISLPMLPVASQEFAPFVFALSVIAIIFTSLTAMMQTDIKKLIAYSSVAHMGFVTLGLF
TLNEQGIQGALFLMISHGIVSGALFLCVGVVYDRMHTREIAAYGGLVKRMPLYAVAMLIFTMANVGLPGTSGFVGEFLAM
LGAFKANPNVAFFSTFGIILSAAYALWLYARVIYGKLEKPNLQGILDLDTREKIILAPLVVLTIYYGVHPAPVLDVFAPS
TDALMASIKTALSNTQTAAAALPIPR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=362, Percent_Identity=35.0828729281768, Blast_Score=202, Evalue=9e-52,
Organism=Homo sapiens, GI251831117, Length=373, Percent_Identity=24.3967828418231, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1788613, Length=511, Percent_Identity=36.5949119373777, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1788827, Length=288, Percent_Identity=28.125, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788831, Length=491, Percent_Identity=25.4582484725051, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788614, Length=445, Percent_Identity=24.9438202247191, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI145693160, Length=476, Percent_Identity=26.6806722689076, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI2367154, Length=290, Percent_Identity=25.8620689655172, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788829, Length=405, Percent_Identity=23.9506172839506, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55506; Mature: 55506

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLGILSGLLIVPLCGAAFILTLNGDEESVARNSRWAALATTVVTFLLSLVAWSRYDAA
CCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
SSSFQLVESHAWLTETIRFKLGVDGFSMPLILLTTFLMPFCIGASWHSIHFRVKEYFVAF
CCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LVLETTMIGVFASLDLLLFYLFFEAGLIPMFLIIGIWGGQRRIYASFKFFLYTLLGSVLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LLAVMAMYWQAGTTDIPTLLQYRFPVQMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVEA
HHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCC
PTAGSVILAGILLKMGGYGFIRISLPMLPVASQEFAPFVFALSVIAIIFTSLTAMMQTDI
CCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLIAYSSVAHMGFVTLGLFTLNEQGIQGALFLMISHGIVSGALFLCVGVVYDRMHTREI
HHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAYGGLVKRMPLYAVAMLIFTMANVGLPGTSGFVGEFLAMLGAFKANPNVAFFSTFGIIL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHH
SAAYALWLYARVIYGKLEKPNLQGILDLDTREKIILAPLVVLTIYYGVHPAPVLDVFAPS
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCC
TDALMASIKTALSNTQTAAAALPIPR
HHHHHHHHHHHHCCCCHHHCCCCCCC
>Mature Secondary Structure
MLGLGILSGLLIVPLCGAAFILTLNGDEESVARNSRWAALATTVVTFLLSLVAWSRYDAA
CCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
SSSFQLVESHAWLTETIRFKLGVDGFSMPLILLTTFLMPFCIGASWHSIHFRVKEYFVAF
CCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LVLETTMIGVFASLDLLLFYLFFEAGLIPMFLIIGIWGGQRRIYASFKFFLYTLLGSVLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LLAVMAMYWQAGTTDIPTLLQYRFPVQMQTWLWLAFFASFAVKMPMWPVHTWLPDAHVEA
HHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCC
PTAGSVILAGILLKMGGYGFIRISLPMLPVASQEFAPFVFALSVIAIIFTSLTAMMQTDI
CCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLIAYSSVAHMGFVTLGLFTLNEQGIQGALFLMISHGIVSGALFLCVGVVYDRMHTREI
HHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAYGGLVKRMPLYAVAMLIFTMANVGLPGTSGFVGEFLAMLGAFKANPNVAFFSTFGIIL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHH
SAAYALWLYARVIYGKLEKPNLQGILDLDTREKIILAPLVVLTIYYGVHPAPVLDVFAPS
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCC
TDALMASIKTALSNTQTAAAALPIPR
HHHHHHHHHHHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]