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The map label for this gene is nuoN [H]

Identifier: 170748464

GI number: 170748464

Start: 2175209

End: 2176654

Strand: Reverse

Name: nuoN [H]

Synonym: Mrad2831_2046

Alternate gene names: 170748464

Gene position: 2176654-2175209 (Counterclockwise)

Preceding gene: 170748465

Following gene: 170748463

Centisome position: 35.81

GC content: 67.5

Gene sequence:

>1446_bases
ATGACAACCGTCCACTCCGTCCTGCCGTCGCTGGCGCCGCTCCTGCCCGAGCTGATCCTCGGCGTCGGCGTCATGGTCCT
GATCCTCTACGGCGCCTGGCGCGGCGACCGCTCCGCCGAGACCGTCAGCGTCGGCGCCCTGGTCCTGCTCCTCGTCGCCC
TGTCGGTGGTGATCTCCCAGCCGGTCCACGGCCGGGTGACGACCCTGTCGGGGGCGTTCGTGTCGGACGCCTTCTCCAAG
GTGATGAAGTCGCTGGTGCTGCTCGGCTCGTCGGCGACGATCCTGCTGGCGCACGACTTCTTCAAGCGCGAGCGCATCGA
CCGGTTCGAGTTCCCGGTGCTGATCGTGCTCTGCACCATCGGCATGATGGTCATGGTCTCGGCCAACGACCTGATCGCGC
TCTATCTCGGTCTGGAGTTGCAGTCGCTCGCCGCCTACGTGATCGCCGCCTTCCACCGGGACGACGTGAAGTCCACCGAG
GCCGGCCTCAAGTACTTCGTGCTCGGCGCGCTCTCCTCCGGCATGCTGCTCTACGGCGCCTCGCTGGTCTACGGCTTCAC
CGGCACCGTGTCGTTCCCCGGCATCGTCTCCGCGCTGAACGAGAATGCCGGCTTCGGCATCGTGCTCGGCATCGTGTTCC
TGGCGGCCGGCGTCTGCTTCAAGCTGGCGGCCGTGCCGTTCCACATGTGGACGCCCGACGTCTACGAGGGCTCGCCGACG
CCGGTGACGGCCTTCTTCGCCTCGGCGCCCAAGATGGCCGCCGTGGCGATGACGGTCCGCGTCTTTATCGGCGCCATGCC
GGACGTGACGGCGATCTGGCAGCAGATCTTCATCTTCGTCTCGGTGGTGTCGATGGCGCTCGGCTCCTTCGCGGCGATCG
GCCAGACCTCGATCAAGCGCCTGATGGCCTACTCGTCGATCGCCAATATCGGCTACGCGCTGATCGGGCTCGCCTGCGGC
TCGGAGGAGGGGATCAGCGGGCTCGTCACCTACATGATCATCTACCTCGCGATGACCCTCGGCACCTTCGCGATCATCCT
GTCGCTCCGGCGCCGGGACGTGATGTTCGAGAAGATCGAGGACCTGTCGGGCCTGTCGCGCACGCATCCGTGGCTGGCCT
TCTGCCTCGCCGCGATGATGTTCTCGCTGGCCGGCATCCCGCCGCTCGCCGGGTTCTTCGCGAAGTTCTACGTCTTCGCC
GCCGCCATCAAGGCGGGCCTCGTGACGCTGGCGGTGATCGGCGTCGTGACCAGCGTGGTCGGCGCCTACTACTACCTGCG
CGTCGTGCGGATCATGTACTTCGACGAGCCGAAGGAGCCCTACGAGGCGATGGCGCCGGGCCTGCGCGTGGTGCTCGCCC
TGTCGAGCGTCGTGGTGGTGCTGTTCTTCCTGCTGCCCGGCCCGCTGGTCGGCGCCGCCGGCCGGGCCGCCAAGTCGCTG
TTCTAG

Upstream 100 bases:

>100_bases
GCGCCCTCGACCGACGCGCTCATGGCGAGCATCAAGACCGCGCTCTCGAACACCCAGACGGCCGCCGCGGCGCTGCCGAT
CCCGCGCTGAGGCGGACACA

Downstream 100 bases:

>100_bases
GACGGCCTTGAGCTACCGACTCGGGCCCTCGGCGCGCGCCGAGGGCCACCGCCTGCACGTCCACGACACCCTCGGCTCCA
CCAACACGGAGGCCATGGCG

Product: proton-translocating NADH-quinone oxidoreductase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MTTVHSVLPSLAPLLPELILGVGVMVLILYGAWRGDRSAETVSVGALVLLLVALSVVISQPVHGRVTTLSGAFVSDAFSK
VMKSLVLLGSSATILLAHDFFKRERIDRFEFPVLIVLCTIGMMVMVSANDLIALYLGLELQSLAAYVIAAFHRDDVKSTE
AGLKYFVLGALSSGMLLYGASLVYGFTGTVSFPGIVSALNENAGFGIVLGIVFLAAGVCFKLAAVPFHMWTPDVYEGSPT
PVTAFFASAPKMAAVAMTVRVFIGAMPDVTAIWQQIFIFVSVVSMALGSFAAIGQTSIKRLMAYSSIANIGYALIGLACG
SEEGISGLVTYMIIYLAMTLGTFAIILSLRRRDVMFEKIEDLSGLSRTHPWLAFCLAAMMFSLAGIPPLAGFFAKFYVFA
AAIKAGLVTLAVIGVVTSVVGAYYYLRVVRIMYFDEPKEPYEAMAPGLRVVLALSSVVVVLFFLLPGPLVGAAGRAAKSL
F

Sequences:

>Translated_481_residues
MTTVHSVLPSLAPLLPELILGVGVMVLILYGAWRGDRSAETVSVGALVLLLVALSVVISQPVHGRVTTLSGAFVSDAFSK
VMKSLVLLGSSATILLAHDFFKRERIDRFEFPVLIVLCTIGMMVMVSANDLIALYLGLELQSLAAYVIAAFHRDDVKSTE
AGLKYFVLGALSSGMLLYGASLVYGFTGTVSFPGIVSALNENAGFGIVLGIVFLAAGVCFKLAAVPFHMWTPDVYEGSPT
PVTAFFASAPKMAAVAMTVRVFIGAMPDVTAIWQQIFIFVSVVSMALGSFAAIGQTSIKRLMAYSSIANIGYALIGLACG
SEEGISGLVTYMIIYLAMTLGTFAIILSLRRRDVMFEKIEDLSGLSRTHPWLAFCLAAMMFSLAGIPPLAGFFAKFYVFA
AAIKAGLVTLAVIGVVTSVVGAYYYLRVVRIMYFDEPKEPYEAMAPGLRVVLALSSVVVVLFFLLPGPLVGAAGRAAKSL
F
>Mature_480_residues
TTVHSVLPSLAPLLPELILGVGVMVLILYGAWRGDRSAETVSVGALVLLLVALSVVISQPVHGRVTTLSGAFVSDAFSKV
MKSLVLLGSSATILLAHDFFKRERIDRFEFPVLIVLCTIGMMVMVSANDLIALYLGLELQSLAAYVIAAFHRDDVKSTEA
GLKYFVLGALSSGMLLYGASLVYGFTGTVSFPGIVSALNENAGFGIVLGIVFLAAGVCFKLAAVPFHMWTPDVYEGSPTP
VTAFFASAPKMAAVAMTVRVFIGAMPDVTAIWQQIFIFVSVVSMALGSFAAIGQTSIKRLMAYSSIANIGYALIGLACGS
EEGISGLVTYMIIYLAMTLGTFAIILSLRRRDVMFEKIEDLSGLSRTHPWLAFCLAAMMFSLAGIPPLAGFFAKFYVFAA
AIKAGLVTLAVIGVVTSVVGAYYYLRVVRIMYFDEPKEPYEAMAPGLRVVLALSSVVVVLFFLLPGPLVGAAGRAAKSLF

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831108, Length=299, Percent_Identity=24.0802675585284, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI145693160, Length=481, Percent_Identity=36.3825363825364, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1788614, Length=381, Percent_Identity=27.8215223097113, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788831, Length=296, Percent_Identity=25.6756756756757, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788829, Length=359, Percent_Identity=26.4623955431755, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788613, Length=221, Percent_Identity=26.2443438914027, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788827, Length=318, Percent_Identity=25.4716981132075, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 51210; Mature: 51079

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVHSVLPSLAPLLPELILGVGVMVLILYGAWRGDRSAETVSVGALVLLLVALSVVISQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PVHGRVTTLSGAFVSDAFSKVMKSLVLLGSSATILLAHDFFKRERIDRFEFPVLIVLCTI
CCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHH
GMMVMVSANDLIALYLGLELQSLAAYVIAAFHRDDVKSTEAGLKYFVLGALSSGMLLYGA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SLVYGFTGTVSFPGIVSALNENAGFGIVLGIVFLAAGVCFKLAAVPFHMWTPDVYEGSPT
HHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PVTAFFASAPKMAAVAMTVRVFIGAMPDVTAIWQQIFIFVSVVSMALGSFAAIGQTSIKR
CHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMAYSSIANIGYALIGLACGSEEGISGLVTYMIIYLAMTLGTFAIILSLRRRDVMFEKIE
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLSGLSRTHPWLAFCLAAMMFSLAGIPPLAGFFAKFYVFAAAIKAGLVTLAVIGVVTSVV
HHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAYYYLRVVRIMYFDEPKEPYEAMAPGLRVVLALSSVVVVLFFLLPGPLVGAAGRAAKSL
HHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHCC
F
C
>Mature Secondary Structure 
TTVHSVLPSLAPLLPELILGVGVMVLILYGAWRGDRSAETVSVGALVLLLVALSVVISQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC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C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA