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The map label for this gene is birA [H]

Identifier: 170748463

GI number: 170748463

Start: 2174398

End: 2175201

Strand: Reverse

Name: birA [H]

Synonym: Mrad2831_2045

Alternate gene names: 170748463

Gene position: 2175201-2174398 (Counterclockwise)

Preceding gene: 170748464

Following gene: 170748462

Centisome position: 35.79

GC content: 75.25

Gene sequence:

>804_bases
TTGAGCTACCGACTCGGGCCCTCGGCGCGCGCCGAGGGCCACCGCCTGCACGTCCACGACACCCTCGGCTCCACCAACAC
GGAGGCCATGGCGCGCGCGCGCGCGGGCGAGACCGGCCCGCTCTGGGTCGTGACGCACCGCCAGGAGACGGGCCGCGGCC
GGCGCGGCAACAGCTGGGCCTCGCTTCCCGGGAACCTCGCGGCCAGCGTGCTCTGGCCCGTTGCCGGGGTCGCGCCGGAC
CACCTCGCCGAACTCGGCTTCGTGGCCGGCCTCGCCCTGATCGAGGCGCTGGAGGCGGCCTGCGGCACCCTGCCGGATAC
CGGATCCGGCGCCGGGGCCCGATCTTTCCGGCTGAAATGGCCGAACGACGTTCTGCTCGGCGGGGCGAAGCTCGCGGGTC
TTCTCCTGGAGGCGGAGACGCTGCCCGGCGGCCGGCGCGCCGCCGTCATCGGCTTCGGCGTGAACGTCGCGGCCGCGCCG
GACGGCCTGGAGGCCGCGAGCTTGGCGGCGGCGGGTTACGCGCGCGACGCCGAGGCGCTACTGGAACATCTCTCGGACCG
GATGGCCGCGCGGGCGCGCGCCTGGGACCGAGGACGGAATTTCGCTGACCTGCGCGCGGCCTGGCTCGCGCGGGCCGCGG
GTCTCGGATCGGAGATCGCGGTGCACAGCGGCGGGCACACCCTGCGGGGCGTGTTCGAGACGATCGACGAGGGGGGGCGG
CTCGTGATCCTCGCCCCCGATGGGAACCGGCATACCGTGACGGCGGGCGAGGTGCATTTCGGATCGGCCGCGACGGCGGC
CTGA

Upstream 100 bases:

>100_bases
CTCGCCCTGTCGAGCGTCGTGGTGGTGCTGTTCTTCCTGCTGCCCGGCCCGCTGGTCGGCGCCGCCGGCCGGGCCGCCAA
GTCGCTGTTCTAGGACGGCC

Downstream 100 bases:

>100_bases
GTTTGGACGACGACAGATGGTTAAAGGACAGGAGGAGCTGGTCTTCCTGCCGCTCGGCGGCGTGGGCGAGATCGGGATGA
ACGCGGCGCTCTACGGGTTC

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSYRLGPSARAEGHRLHVHDTLGSTNTEAMARARAGETGPLWVVTHRQETGRGRRGNSWASLPGNLAASVLWPVAGVAPD
HLAELGFVAGLALIEALEAACGTLPDTGSGAGARSFRLKWPNDVLLGGAKLAGLLLEAETLPGGRRAAVIGFGVNVAAAP
DGLEAASLAAAGYARDAEALLEHLSDRMAARARAWDRGRNFADLRAAWLARAAGLGSEIAVHSGGHTLRGVFETIDEGGR
LVILAPDGNRHTVTAGEVHFGSAATAA

Sequences:

>Translated_267_residues
MSYRLGPSARAEGHRLHVHDTLGSTNTEAMARARAGETGPLWVVTHRQETGRGRRGNSWASLPGNLAASVLWPVAGVAPD
HLAELGFVAGLALIEALEAACGTLPDTGSGAGARSFRLKWPNDVLLGGAKLAGLLLEAETLPGGRRAAVIGFGVNVAAAP
DGLEAASLAAAGYARDAEALLEHLSDRMAARARAWDRGRNFADLRAAWLARAAGLGSEIAVHSGGHTLRGVFETIDEGGR
LVILAPDGNRHTVTAGEVHFGSAATAA
>Mature_266_residues
SYRLGPSARAEGHRLHVHDTLGSTNTEAMARARAGETGPLWVVTHRQETGRGRRGNSWASLPGNLAASVLWPVAGVAPDH
LAELGFVAGLALIEALEAACGTLPDTGSGAGARSFRLKWPNDVLLGGAKLAGLLLEAETLPGGRRAAVIGFGVNVAAAPD
GLEAASLAAAGYARDAEALLEHLSDRMAARARAWDRGRNFADLRAAWLARAAGLGSEIAVHSGGHTLRGVFETIDEGGRL
VILAPDGNRHTVTAGEVHFGSAATAA

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=273, Percent_Identity=27.4725274725275, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 27518; Mature: 27387

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYRLGPSARAEGHRLHVHDTLGSTNTEAMARARAGETGPLWVVTHRQETGRGRRGNSWA
CCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECHHCCCCCCCCCCC
SLPGNLAASVLWPVAGVAPDHLAELGFVAGLALIEALEAACGTLPDTGSGAGARSFRLKW
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEC
PNDVLLGGAKLAGLLLEAETLPGGRRAAVIGFGVNVAAAPDGLEAASLAAAGYARDAEAL
CCCEEECCHHHHHHEEECCCCCCCCEEEEEEECCEEEECCCCCHHHHHHHHCCCCCHHHH
LEHLSDRMAARARAWDRGRNFADLRAAWLARAAGLGSEIAVHSGGHTLRGVFETIDEGGR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCE
LVILAPDGNRHTVTAGEVHFGSAATAA
EEEEECCCCCEEEEECCEECCCCCCCC
>Mature Secondary Structure 
SYRLGPSARAEGHRLHVHDTLGSTNTEAMARARAGETGPLWVVTHRQETGRGRRGNSWA
CCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECHHCCCCCCCCCCC
SLPGNLAASVLWPVAGVAPDHLAELGFVAGLALIEALEAACGTLPDTGSGAGARSFRLKW
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEC
PNDVLLGGAKLAGLLLEAETLPGGRRAAVIGFGVNVAAAPDGLEAASLAAAGYARDAEAL
CCCEEECCHHHHHHEEECCCCCCCCEEEEEEECCEEEECCCCCHHHHHHHHCCCCCHHHH
LEHLSDRMAARARAWDRGRNFADLRAAWLARAAGLGSEIAVHSGGHTLRGVFETIDEGGR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCE
LVILAPDGNRHTVTAGEVHFGSAATAA
EEEEECCCCCEEEEECCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]