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The map label for this gene is proS

Identifier: 170748459

GI number: 170748459

Start: 2169910

End: 2171235

Strand: Reverse

Name: proS

Synonym: Mrad2831_2041

Alternate gene names: 170748459

Gene position: 2171235-2169910 (Counterclockwise)

Preceding gene: 170748460

Following gene: 170748458

Centisome position: 35.72

GC content: 69.31

Gene sequence:

>1326_bases
ATGCGTCTCTCCCGCTACTTCATGCCGACCTTGCGCGAGACGCCCAAGGAAGCCGAGATCGTCTCGCACCGGCTGATGCT
GCGCGCCGGCCTGGTCCGCCAGGAAGCCGCCGGCATCTACGCCTGGCTGCCGCTGGGCCTGCGGGTGCTGGAGCGCGTCT
GCGCGGTGATCCGGCGGGAGCAGGACCGGTCCGGCGCCGTCGAGATCCTGATGCCGACGGTGCAGTCCGCCGAGCTGTGG
CGGGAATCCGGCCGCTACGACGCCTACGGCAAGGAGATGCTGCGCCTGAAGGACCGGCACGACCGCGAGATGCTCTACGG
CCCCACCGCCGAGGAGATGGTCACCGAGATCTTCCGGGCGAGCGTGCGCTCCTACAAGGACCTGCCCAAGAACCTCTACC
AGATCTCGTGGAAGTTCCGCGACGAGGTGCGGCCGCGCTTCGGCACGATGCGCTCCCGCGAGTTCCTGATGAAGGACGCC
TACTCGTTCGACTTCGACCAGGCCGGCGCCCGCCACGCCTACAACCGGATGTTCGTGGCGTATCTGCGCACCTTCGCGGG
CCTCGGCCTGAAGGCGATCCCGATGCGCGCCGATACCGGCCCGATCGGCGGCGACCTGTCCCACGAGTTCATCATCCTGG
CGAAGACCGGCGAGAGCGAGGTCTTCTGCGACAAGGCCTATCTCGGCTTCGACATCCCGCCGGCGACCGTCGATTTCGAC
GACGTCGCCGCCCTGCAGGGCATCGTCGATGCCTGGACCTCGCACTACGCGGCCACCGAGGAGATGCACGAGCCGGCGGC
CTTCGCGGAGGTCCCGGAGGACAGCCGCATGGCCGCCCGCGGCATCGAGGTTGGGCACATCTTCTATTTCGGAACGAAGT
ATTCCGAGCCGATGGGCGCCAAGGTCGCCGGTCCGGACGGGATCGAGCGGCCGGTCCACATGGGCTCCTACGGCATCGGG
CCGAGCCGCCTGGTGGCGGCGATCATCGAGGCCAGCCACGACGAGGCCGGGATCATCTGGCCGGACGCGGTCGCGCCGTT
CGACGTCGCGCTGATCAACCTCAAGGTCGGGGATTCCGCGACCGAAGCCGCCTGCGCGCAGATCCAGTCCGATCTCGAGG
CCGCCGGCCTGTCGGTGCTCTACGACGACCGCGACGAGCGCCCCGGCGCGAAGTTCGCCACCGCCGACCTGATCGGCCTG
CCCTGGCAGGTGATCGTCGGGCCCAAGGGCCTGGCGGAGGGCAAGGTCGAGCTGAAGCGGCGTGCCGGCGGCGAGCGCGA
GAGCCTCGCGACCGTCGATCTGCTGGCACGCATCCGGCGCGTCTGA

Upstream 100 bases:

>100_bases
CGCCGTGGTCGCGCAGTGCTTGTAATTTGCGTCTCCGGCGTGTTGTGTGCCGGCGCGCCGCGGCTCCTCCCGGTCGATCC
TCTTCCAAGACTCCGAAGAC

Downstream 100 bases:

>100_bases
GGCGTGCCGATGGCCGCCTCAGCGAAGCTGGACCGGGTTCGGACCTTCGCGGCCTCGCTCGCGGCCTCGTTCCGCCAGGG
CACCGCGCCCTTCGCGCCGT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MRLSRYFMPTLRETPKEAEIVSHRLMLRAGLVRQEAAGIYAWLPLGLRVLERVCAVIRREQDRSGAVEILMPTVQSAELW
RESGRYDAYGKEMLRLKDRHDREMLYGPTAEEMVTEIFRASVRSYKDLPKNLYQISWKFRDEVRPRFGTMRSREFLMKDA
YSFDFDQAGARHAYNRMFVAYLRTFAGLGLKAIPMRADTGPIGGDLSHEFIILAKTGESEVFCDKAYLGFDIPPATVDFD
DVAALQGIVDAWTSHYAATEEMHEPAAFAEVPEDSRMAARGIEVGHIFYFGTKYSEPMGAKVAGPDGIERPVHMGSYGIG
PSRLVAAIIEASHDEAGIIWPDAVAPFDVALINLKVGDSATEAACAQIQSDLEAAGLSVLYDDRDERPGAKFATADLIGL
PWQVIVGPKGLAEGKVELKRRAGGERESLATVDLLARIRRV

Sequences:

>Translated_441_residues
MRLSRYFMPTLRETPKEAEIVSHRLMLRAGLVRQEAAGIYAWLPLGLRVLERVCAVIRREQDRSGAVEILMPTVQSAELW
RESGRYDAYGKEMLRLKDRHDREMLYGPTAEEMVTEIFRASVRSYKDLPKNLYQISWKFRDEVRPRFGTMRSREFLMKDA
YSFDFDQAGARHAYNRMFVAYLRTFAGLGLKAIPMRADTGPIGGDLSHEFIILAKTGESEVFCDKAYLGFDIPPATVDFD
DVAALQGIVDAWTSHYAATEEMHEPAAFAEVPEDSRMAARGIEVGHIFYFGTKYSEPMGAKVAGPDGIERPVHMGSYGIG
PSRLVAAIIEASHDEAGIIWPDAVAPFDVALINLKVGDSATEAACAQIQSDLEAAGLSVLYDDRDERPGAKFATADLIGL
PWQVIVGPKGLAEGKVELKRRAGGERESLATVDLLARIRRV
>Mature_441_residues
MRLSRYFMPTLRETPKEAEIVSHRLMLRAGLVRQEAAGIYAWLPLGLRVLERVCAVIRREQDRSGAVEILMPTVQSAELW
RESGRYDAYGKEMLRLKDRHDREMLYGPTAEEMVTEIFRASVRSYKDLPKNLYQISWKFRDEVRPRFGTMRSREFLMKDA
YSFDFDQAGARHAYNRMFVAYLRTFAGLGLKAIPMRADTGPIGGDLSHEFIILAKTGESEVFCDKAYLGFDIPPATVDFD
DVAALQGIVDAWTSHYAATEEMHEPAAFAEVPEDSRMAARGIEVGHIFYFGTKYSEPMGAKVAGPDGIERPVHMGSYGIG
PSRLVAAIIEASHDEAGIIWPDAVAPFDVALINLKVGDSATEAACAQIQSDLEAAGLSVLYDDRDERPGAKFATADLIGL
PWQVIVGPKGLAEGKVELKRRAGGERESLATVDLLARIRRV

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=445, Percent_Identity=34.6067415730337, Blast_Score=241, Evalue=9e-64,
Organism=Escherichia coli, GI1786392, Length=227, Percent_Identity=53.7444933920705, Blast_Score=268, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI115532348, Length=408, Percent_Identity=33.3333333333333, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI193203271, Length=91, Percent_Identity=35.1648351648352, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=218, Percent_Identity=35.3211009174312, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24656200, Length=440, Percent_Identity=32.2727272727273, Blast_Score=217, Evalue=2e-56,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_METRJ (B1LUL8)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001754719.1
- ProteinModelPortal:   B1LUL8
- SMR:   B1LUL8
- GeneID:   6138070
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_2041
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK12325
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 49112; Mature: 49112

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRYFMPTLRETPKEAEIVSHRLMLRAGLVRQEAAGIYAWLPLGLRVLERVCAVIRRE
CCCCHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHC
QDRSGAVEILMPTVQSAELWRESGRYDAYGKEMLRLKDRHDREMLYGPTAEEMVTEIFRA
CCCCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHH
SVRSYKDLPKNLYQISWKFRDEVRPRFGTMRSREFLMKDAYSFDFDQAGARHAYNRMFVA
HHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHH
YLRTFAGLGLKAIPMRADTGPIGGDLSHEFIILAKTGESEVFCDKAYLGFDIPPATVDFD
HHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEECCEECCCCCCCCCCHH
DVAALQGIVDAWTSHYAATEEMHEPAAFAEVPEDSRMAARGIEVGHIFYFGTKYSEPMGA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHCCCEEEEEEEECCCCCCCCCC
KVAGPDGIERPVHMGSYGIGPSRLVAAIIEASHDEAGIIWPDAVAPFDVALINLKVGDSA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEEEEEECCCH
TEAACAQIQSDLEAAGLSVLYDDRDERPGAKFATADLIGLPWQVIVGPKGLAEGKVELKR
HHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEHHHHHCCCEEEEECCCCCCCCHHHHHH
RAGGERESLATVDLLARIRRV
HCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLSRYFMPTLRETPKEAEIVSHRLMLRAGLVRQEAAGIYAWLPLGLRVLERVCAVIRRE
CCCCHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHC
QDRSGAVEILMPTVQSAELWRESGRYDAYGKEMLRLKDRHDREMLYGPTAEEMVTEIFRA
CCCCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHH
SVRSYKDLPKNLYQISWKFRDEVRPRFGTMRSREFLMKDAYSFDFDQAGARHAYNRMFVA
HHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHH
YLRTFAGLGLKAIPMRADTGPIGGDLSHEFIILAKTGESEVFCDKAYLGFDIPPATVDFD
HHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEECCEECCCCCCCCCCHH
DVAALQGIVDAWTSHYAATEEMHEPAAFAEVPEDSRMAARGIEVGHIFYFGTKYSEPMGA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHCCCEEEEEEEECCCCCCCCCC
KVAGPDGIERPVHMGSYGIGPSRLVAAIIEASHDEAGIIWPDAVAPFDVALINLKVGDSA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEEEEEECCCH
TEAACAQIQSDLEAAGLSVLYDDRDERPGAKFATADLIGLPWQVIVGPKGLAEGKVELKR
HHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEHHHHHCCCEEEEECCCCCCCCHHHHHH
RAGGERESLATVDLLARIRRV
HCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA