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The map label for this gene is intA [H]

Identifier: 170748415

GI number: 170748415

Start: 2125227

End: 2126429

Strand: Reverse

Name: intA [H]

Synonym: Mrad2831_1997

Alternate gene names: 170748415

Gene position: 2126429-2125227 (Counterclockwise)

Preceding gene: 170748417

Following gene: 170748414

Centisome position: 34.99

GC content: 68.41

Gene sequence:

>1203_bases
ATGCAGGGTAAGCTCACGGCCCTCGGCGTGGCTCGGCTGAAGATGCCGGGCATGTACGGCGACGGACACGGCCTCTGGCT
TCAGGTGACCGGCAAGGGCGGGAAGAGCTGGATCTTCCGGTACAAAATCGCCGGCCGGGCGCGGGAGATGGGGCTCGGGT
CGTGCGCGACCTGGTCGCTCGCCGAAGCGCGCGAGCGGGCGCGGGAATGCCGGAAGCTGACCTCGGAGGGCATCGACCCG
ATCGATGTCCGCAAAGTGAAGCGCCAGGAGGCAGCCCTAGAGGCCGCGAAGGCCATCACCTTCCGCGCCTGCGCCGAGAA
GTACATCGAGGCCCACAAGGCCGGCTGGCGCAACGCCATCCACGCGGCGCAATGGCCGTCGTCGCTGGAGAACCACGTCC
ATCCGGTGATCGGCGACCTTCCGGTCCAGGCCATCGACACCGGCTCGGTGATGAAGGTGCTGGAGCCGATCTGGGCCACG
CGATCCGAGACGGCGACGAGGGTGCGCGCCCGGATCGAGTCGGTGCTCGATTGGGCGGCTACCCATGGCTACCGGCGCGG
CGAGAACCCTGCGCGCTGGCGCGGGCACCTGCAGAACCTCCTTCCGAAACGGTCGCGGGTGAAGCGGGTGCAGCACTACG
CGGCGCTGCCGTTCAAGGAGATGCCCGCCTTCATGGTCGACCTTCGAGGCGAGGACTCTGTCCCTGCGCGCGCGCTGGAG
TTCGCGATCCTGACGGCAGCCCGCTCGGGCGAGGCGCTGGGCGCACGCTGGTCCGAGATCGACATGGAAGCTGGGATCTG
GGCCATACCGGCCGAGCGGATGAAATCCGGCGTCGAGCACCGGGTGCCGCTGTCGGCGCCGGCCATGGCTCTCTTGGCGA
AGATGCGCGGCCTCAACGAGACCTTCGTGTTTCCGGGCAGCCGGAGCCGGCGGCCGCTCACGGACAAGATGGCGCGCGAC
GTCCTCGTGAAGATGGACCGAGCCGGACTGACCGTTCACGGCTTCCGATCGTCGTTCCGGGACTGGGCCGGTGAGTGCAC
CTCGTTCCCCCGTGACCTCGCTGAGATGGCTTTGGCTCACACCGTGGGCGACTCGACCGAGCGGGCCTATCGGCGCGGCG
ACCTATTTGAGAAGCGGCGCGAGTTGATGGACGCCTGGGCTCAACACGCTGGTCCGCCGTCTGCGCATAAGCGGGGCGCA
TAA

Upstream 100 bases:

>100_bases
GAAAAACTGGAGCTATTCCGGGACAGGAGTTCGCAGAAGTGCGCTGGCGTTCCCACAAATCCGCGTTTACGGTGTGGGTA
GAAGCGCGGGTGAAGATCCT

Downstream 100 bases:

>100_bases
GATGAGCGATGACGCCGACAACGACGCAGCAGTCGACGCGCTTCTTGCTGTCGCAGCACCAGCGTTCAATGCGAAAGCCT
CTCCCTATTTTACTGAGGTC

Product: integrase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MQGKLTALGVARLKMPGMYGDGHGLWLQVTGKGGKSWIFRYKIAGRAREMGLGSCATWSLAEARERARECRKLTSEGIDP
IDVRKVKRQEAALEAAKAITFRACAEKYIEAHKAGWRNAIHAAQWPSSLENHVHPVIGDLPVQAIDTGSVMKVLEPIWAT
RSETATRVRARIESVLDWAATHGYRRGENPARWRGHLQNLLPKRSRVKRVQHYAALPFKEMPAFMVDLRGEDSVPARALE
FAILTAARSGEALGARWSEIDMEAGIWAIPAERMKSGVEHRVPLSAPAMALLAKMRGLNETFVFPGSRSRRPLTDKMARD
VLVKMDRAGLTVHGFRSSFRDWAGECTSFPRDLAEMALAHTVGDSTERAYRRGDLFEKRRELMDAWAQHAGPPSAHKRGA

Sequences:

>Translated_400_residues
MQGKLTALGVARLKMPGMYGDGHGLWLQVTGKGGKSWIFRYKIAGRAREMGLGSCATWSLAEARERARECRKLTSEGIDP
IDVRKVKRQEAALEAAKAITFRACAEKYIEAHKAGWRNAIHAAQWPSSLENHVHPVIGDLPVQAIDTGSVMKVLEPIWAT
RSETATRVRARIESVLDWAATHGYRRGENPARWRGHLQNLLPKRSRVKRVQHYAALPFKEMPAFMVDLRGEDSVPARALE
FAILTAARSGEALGARWSEIDMEAGIWAIPAERMKSGVEHRVPLSAPAMALLAKMRGLNETFVFPGSRSRRPLTDKMARD
VLVKMDRAGLTVHGFRSSFRDWAGECTSFPRDLAEMALAHTVGDSTERAYRRGDLFEKRRELMDAWAQHAGPPSAHKRGA
>Mature_400_residues
MQGKLTALGVARLKMPGMYGDGHGLWLQVTGKGGKSWIFRYKIAGRAREMGLGSCATWSLAEARERARECRKLTSEGIDP
IDVRKVKRQEAALEAAKAITFRACAEKYIEAHKAGWRNAIHAAQWPSSLENHVHPVIGDLPVQAIDTGSVMKVLEPIWAT
RSETATRVRARIESVLDWAATHGYRRGENPARWRGHLQNLLPKRSRVKRVQHYAALPFKEMPAFMVDLRGEDSVPARALE
FAILTAARSGEALGARWSEIDMEAGIWAIPAERMKSGVEHRVPLSAPAMALLAKMRGLNETFVFPGSRSRRPLTDKMARD
VLVKMDRAGLTVHGFRSSFRDWAGECTSFPRDLAEMALAHTVGDSTERAYRRGDLFEKRRELMDAWAQHAGPPSAHKRGA

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=376, Percent_Identity=30.8510638297872, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI145693166, Length=373, Percent_Identity=32.9758713136729, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788690, Length=378, Percent_Identity=29.8941798941799, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 44588; Mature: 44588

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGKLTALGVARLKMPGMYGDGHGLWLQVTGKGGKSWIFRYKIAGRAREMGLGSCATWSL
CCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCHHHCCCCCCCHHHH
AEARERARECRKLTSEGIDPIDVRKVKRQEAALEAAKAITFRACAEKYIEAHKAGWRNAI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAAQWPSSLENHVHPVIGDLPVQAIDTGSVMKVLEPIWATRSETATRVRARIESVLDWAA
HHHHCHHHHHHCCCHHHCCCCCHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
THGYRRGENPARWRGHLQNLLPKRSRVKRVQHYAALPFKEMPAFMVDLRGEDSVPARALE
HCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCHHEEEECCCCCCCHHHHH
FAILTAARSGEALGARWSEIDMEAGIWAIPAERMKSGVEHRVPLSAPAMALLAKMRGLNE
HHHHHHHCCCCHHCCCHHHHHHCCCEEECCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCC
TFVFPGSRSRRPLTDKMARDVLVKMDRAGLTVHGFRSSFRDWAGECTSFPRDLAEMALAH
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHH
TVGDSTERAYRRGDLFEKRRELMDAWAQHAGPPSAHKRGA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQGKLTALGVARLKMPGMYGDGHGLWLQVTGKGGKSWIFRYKIAGRAREMGLGSCATWSL
CCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCHHHCCCCCCCHHHH
AEARERARECRKLTSEGIDPIDVRKVKRQEAALEAAKAITFRACAEKYIEAHKAGWRNAI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAAQWPSSLENHVHPVIGDLPVQAIDTGSVMKVLEPIWATRSETATRVRARIESVLDWAA
HHHHCHHHHHHCCCHHHCCCCCHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
THGYRRGENPARWRGHLQNLLPKRSRVKRVQHYAALPFKEMPAFMVDLRGEDSVPARALE
HCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCHHEEEECCCCCCCHHHHH
FAILTAARSGEALGARWSEIDMEAGIWAIPAERMKSGVEHRVPLSAPAMALLAKMRGLNE
HHHHHHHCCCCHHCCCHHHHHHCCCEEECCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCC
TFVFPGSRSRRPLTDKMARDVLVKMDRAGLTVHGFRSSFRDWAGECTSFPRDLAEMALAH
EEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHH
TVGDSTERAYRRGDLFEKRRELMDAWAQHAGPPSAHKRGA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]