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The map label for this gene is citH [H]

Identifier: 170747103

GI number: 170747103

Start: 716968

End: 718284

Strand: Reverse

Name: citH [H]

Synonym: Mrad2831_0669

Alternate gene names: 170747103

Gene position: 718284-716968 (Counterclockwise)

Preceding gene: 170747104

Following gene: 170747102

Centisome position: 11.82

GC content: 72.67

Gene sequence:

>1317_bases
ATGGACGCCCGGACCGCACCGCATCCCGGCGCGCATCATCCCGCCCTCGACCGCAAGTCGGTCGGGGCCGTCGTCCTGGG
CAACGCCCTCGAGTTCTACGATTTCACCGTCTACGCCTTCTTCGCCAAGCCGATCGGCGAGGCCTTCTTCCCGGCGAACG
ACCCGACCCACAGCCTGCTGGCATCGCTCGCCCTGTTCGGCATCGGCTACGTCATGCGGCCCATCGGCGGCGTGCTGATC
GGCGCTTTCGCGGACCGGGCCGGCCGCAAGCCCGCGATGCTGATCACCATCGCGCTGATGGCGGTCGGCATGGTCCTGCT
GGCCGCCTGCCCCTCCTACGCGACGATCGGCGGCTGGGCGCAGGTCATCGTCATTGCCGGCCGCCTCGTGCAGGGTCTCG
CCCTCGGCGGCGAGGTCGGCCCCTCGACGGCCTTCCTGCTGGAAGCCGCGCCCCCGGACCGGCGCGGCTTCGTGACGAGC
TGGCAGATCGCCAGCCAGGGCTGCGCGGCCCTGTTCGCCGGGATCGTCGCGACCGCCCTGGCGCTCGCGGTCGGCGATCA
GGCGATGGCGCAGTGGGGCTGGCGGCTGATGTTCGCCCTCGGCCTGTGCGTCGTGCCGGTCGGCCTCGTCATCCGCAGTC
ACCTGCCCGAGACGGCCGGCGCCGAGGAGGATCCGCACGCGGCGGCCTCGACGACGGCGGTGGTCGGCCGGCTCCTGCGC
GAGCACGGCCGCCTGCTGGGCCTGACCTTCCTGGTCATCGCGGCCTCGACCGTGTCGAACGCGGTCGGCACCAACATGCC
GGTCTTCGCCGGCGCAACCCTCGGCCTGAGCGAGACCGCCGCCACGGCGGTGCCGATCGCGCTCGGGCTCGCCTCGGTGA
TCTTCCCGCTGCTCGGCGGCTGGCTGGCCGACCGGTACGGGCGGCGACCGGTGATGATCTGGCCGCGGGCGCTGATCCTG
ATCCTGGCGATCCCGGCCTTCGCCTGGGTGGTGCGGGCGCCGAGCGCCGCCAGCGTGTACGCGGTCACCTTCCTGCTCTC
GGCCCTGTCGTCCATCAACGCCGCGGCGGTGATCGTCGGCATCCCCGAGGCCCTGCCCCGGGCGGTGCGCAGCGCCGGAT
TGTCGATCGTCTACGCGGTCTCCGTCTCGGTGTTCGGCGGCAGCACCAACTATGTGGTGAACTGGCTGATCGCCGCGACC
GGCGACCGGCTCGCGCCGGCCTACTACCTCGCCGCGTTCAGCCTCGTCGGGACGATCGCGGCTGTCCTGCTGCCCGAGAC
GCGCGGGCGCGACCTCGACGCCGATTCCGAGGCGTGA

Upstream 100 bases:

>100_bases
GCACGCTCTGGTTGTCCGGCTACGACGAGGCGCTGGCTGAGGGCGAGACGCCCGTCACCGGCGGTCTCGCCAAGCACCCG
GTCGACGGATCGTCCCGCTG

Downstream 100 bases:

>100_bases
TCGGCAAATTCGGGTCATCGCGGATTATGTATGCACGGATCGCATCGCGGCGCCTCATCCATGCGTCCGACGCGGAGCCC
GCGCCCCGGGTATGTGCGCT

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MDARTAPHPGAHHPALDRKSVGAVVLGNALEFYDFTVYAFFAKPIGEAFFPANDPTHSLLASLALFGIGYVMRPIGGVLI
GAFADRAGRKPAMLITIALMAVGMVLLAACPSYATIGGWAQVIVIAGRLVQGLALGGEVGPSTAFLLEAAPPDRRGFVTS
WQIASQGCAALFAGIVATALALAVGDQAMAQWGWRLMFALGLCVVPVGLVIRSHLPETAGAEEDPHAAASTTAVVGRLLR
EHGRLLGLTFLVIAASTVSNAVGTNMPVFAGATLGLSETAATAVPIALGLASVIFPLLGGWLADRYGRRPVMIWPRALIL
ILAIPAFAWVVRAPSAASVYAVTFLLSALSSINAAAVIVGIPEALPRAVRSAGLSIVYAVSVSVFGGSTNYVVNWLIAAT
GDRLAPAYYLAAFSLVGTIAAVLLPETRGRDLDADSEA

Sequences:

>Translated_438_residues
MDARTAPHPGAHHPALDRKSVGAVVLGNALEFYDFTVYAFFAKPIGEAFFPANDPTHSLLASLALFGIGYVMRPIGGVLI
GAFADRAGRKPAMLITIALMAVGMVLLAACPSYATIGGWAQVIVIAGRLVQGLALGGEVGPSTAFLLEAAPPDRRGFVTS
WQIASQGCAALFAGIVATALALAVGDQAMAQWGWRLMFALGLCVVPVGLVIRSHLPETAGAEEDPHAAASTTAVVGRLLR
EHGRLLGLTFLVIAASTVSNAVGTNMPVFAGATLGLSETAATAVPIALGLASVIFPLLGGWLADRYGRRPVMIWPRALIL
ILAIPAFAWVVRAPSAASVYAVTFLLSALSSINAAAVIVGIPEALPRAVRSAGLSIVYAVSVSVFGGSTNYVVNWLIAAT
GDRLAPAYYLAAFSLVGTIAAVLLPETRGRDLDADSEA
>Mature_438_residues
MDARTAPHPGAHHPALDRKSVGAVVLGNALEFYDFTVYAFFAKPIGEAFFPANDPTHSLLASLALFGIGYVMRPIGGVLI
GAFADRAGRKPAMLITIALMAVGMVLLAACPSYATIGGWAQVIVIAGRLVQGLALGGEVGPSTAFLLEAAPPDRRGFVTS
WQIASQGCAALFAGIVATALALAVGDQAMAQWGWRLMFALGLCVVPVGLVIRSHLPETAGAEEDPHAAASTTAVVGRLLR
EHGRLLGLTFLVIAASTVSNAVGTNMPVFAGATLGLSETAATAVPIALGLASVIFPLLGGWLADRYGRRPVMIWPRALIL
ILAIPAFAWVVRAPSAASVYAVTFLLSALSSINAAAVIVGIPEALPRAVRSAGLSIVYAVSVSVFGGSTNYVVNWLIAAT
GDRLAPAYYLAAFSLVGTIAAVLLPETRGRDLDADSEA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=437, Percent_Identity=30.6636155606407, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1788942, Length=421, Percent_Identity=32.541567695962, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1788292, Length=434, Percent_Identity=29.0322580645161, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1789941, Length=449, Percent_Identity=32.293986636971, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI87082231, Length=269, Percent_Identity=24.907063197026, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082404, Length=389, Percent_Identity=24.4215938303342, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45202; Mature: 45202

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDARTAPHPGAHHPALDRKSVGAVVLGNALEFYDFTVYAFFAKPIGEAFFPANDPTHSLL
CCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
ASLALFGIGYVMRPIGGVLIGAFADRAGRKPAMLITIALMAVGMVLLAACPSYATIGGWA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
QVIVIAGRLVQGLALGGEVGPSTAFLLEAAPPDRRGFVTSWQIASQGCAALFAGIVATAL
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
ALAVGDQAMAQWGWRLMFALGLCVVPVGLVIRSHLPETAGAEEDPHAAASTTAVVGRLLR
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
EHGRLLGLTFLVIAASTVSNAVGTNMPVFAGATLGLSETAATAVPIALGLASVIFPLLGG
HCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
WLADRYGRRPVMIWPRALILILAIPAFAWVVRAPSAASVYAVTFLLSALSSINAAAVIVG
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
IPEALPRAVRSAGLSIVYAVSVSVFGGSTNYVVNWLIAATGDRLAPAYYLAAFSLVGTIA
CHHHHHHHHHHCCCEEEEEEEEEEECCCCHHHHEEHHHHCCCCCHHHHHHHHHHHHHHHH
AVLLPETRGRDLDADSEA
HHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDARTAPHPGAHHPALDRKSVGAVVLGNALEFYDFTVYAFFAKPIGEAFFPANDPTHSLL
CCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
ASLALFGIGYVMRPIGGVLIGAFADRAGRKPAMLITIALMAVGMVLLAACPSYATIGGWA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
QVIVIAGRLVQGLALGGEVGPSTAFLLEAAPPDRRGFVTSWQIASQGCAALFAGIVATAL
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
ALAVGDQAMAQWGWRLMFALGLCVVPVGLVIRSHLPETAGAEEDPHAAASTTAVVGRLLR
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
EHGRLLGLTFLVIAASTVSNAVGTNMPVFAGATLGLSETAATAVPIALGLASVIFPLLGG
HCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
WLADRYGRRPVMIWPRALILILAIPAFAWVVRAPSAASVYAVTFLLSALSSINAAAVIVG
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
IPEALPRAVRSAGLSIVYAVSVSVFGGSTNYVVNWLIAATGDRLAPAYYLAAFSLVGTIA
CHHHHHHHHHHCCCEEEEEEEEEEECCCCHHHHEEHHHHCCCCCHHHHHHHHHHHHHHHH
AVLLPETRGRDLDADSEA
HHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]