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The map label for this gene is glcA [H]

Identifier: 170747074

GI number: 170747074

Start: 688034

End: 689770

Strand: Reverse

Name: glcA [H]

Synonym: Mrad2831_0640

Alternate gene names: 170747074

Gene position: 689770-688034 (Counterclockwise)

Preceding gene: 170747075

Following gene: 170747073

Centisome position: 11.35

GC content: 69.08

Gene sequence:

>1737_bases
GTGCAAACCTGGAACCAGGTTTATGATCCGTTCGGGAGCCCGTGGCTCTCGACGCTCGCCGCTTCAGTGCCGGTCATCGC
ACTGCTCGCCATGATCGCCAGCGGGCGCGTGAAGGCCCATATCGCGGCCGTCATCGCTCTCGGCCTCGCCATGCTGGTGG
CCATCGTCGGCTTCGGCATGCCGACCGATCTCGCCACCCGCGCCGCCATCCTCGGGATGGTCACGGGCTTCTTCCCGATC
GGCTGGATCATCCTCAACGTGATCTTCCTCTACCGGCTGACGGTGGAGAAGGGCTGGTTCGCGATCCTGCAGCAATCGGT
GGCCGGCATCACCGCCGACCGGCGGTTGCAGCTGCTCCTGGTGGCCTTCGCGTTCGGCGCCTTCTTCGAGGGCGCGGGCG
GCTTCGGCACGCCGGTGGCCGTCACGGGCGCGATCCTGATCGGCCTGGGCTTCTCGCCGCTCGCCGCCTCCGGCCTGTCG
CTGATCGCCAACACCGCCCCGGTGGCCTTCGGGGCGCTCGGCGCGCCGATCCAGGGCCTCGCCTCGGTCACCGGCTACCC
GCCGGAGATCCTCGGCGCGATGATCGGCCGGCAGCTGCCGCTGTTCTCGCTGATCGTGCCGTTCTGGCTGATCTGGGTCT
TCGCGGGCTTCCGCGGCATGATCCGGGTGTGGCCGCCGATCCTGGTCTGCGGCGCCTCCTTCGCGGTCGCGCAGTTCCTG
ATCTCGAACTACGTCAACCCGTGGATCGTCGATATCGGCGCCTCGCTCGCCTCGATGCTGGCCCTCGTGCTGTTCCTGAA
GGTCTGGCAGCCCAAGGAGATCTGGTCGTCGCCGGCCCTGCGCGGCCACGATCCGTCGCTCGCCGTCGCCGGCCCGCGGC
CCGTGGCGCTCGGCGCCGGCGTGCCCGGCACGCCCCCCGTCCATGTCGGCGCCCGCACCGCCTCGCAGGGCGAGATCCTG
ATGGCCTGGGTGCCGTGGATCGTCCTCTCGGTCATCGTCGCGATCTGGGGGACCGGCTGGTTCAAGGGCATCGTCAACCC
GCTCTTCACCTGGAAGTACGAGGTGCCGGGCCTGCACAACGTGATCATGAAGGTGCCGCCGGTGGTGGCCAAGCCCGTCC
CGGAGGGCGCGGTCTTCCTGTTCACCTACATGTCGTACACCGGCACGGGCGTGCTGATCGCGGCGATCATCTCCGGCCTG
ATCATGCGGTTCTCGCCGCTGCGCCTCGTCACGGCCTACTTCGAGACCATCTGGGCCCTGCGCTACTCGCTGATCACCAT
CGCGGCGATGCTCGCGCTCGGCGTCCTCACCCGCTACGCGGGCGTCGACGCCACGCTCGGCCTCGCCTTCGCGGGCACCG
GCATCCTCTACCCGTTCTTCGGGACGCTGCTCGGCTGGCTCGGCGTGGCGCTCACGGGTTCGGACACGGCCTCGAACGTG
CTGTTCGGCGGCCTCCAGAGGATCACCTCCGAGCAGCTCGGCCTCTCGGGCGTGCTGATGGCCGCCGCGAACTCGTCGGG
CGGCGTGATGGGCAAGATGGTCGACGCCCAGTCGATCGTCGTCGCCTCCACGGCGACCGGCTATTTCGGTCAGGAAGGCA
AGATCCTGCGCTTCGTGTTCTGGCACTCGATCGCGCTCGCCTGCCTCGTCGGCCTGTTCGTGATGCTGCAGGCCTACGTG
CCGTTCTTCCAGCAGATGGTCATCGACCTGCCCGCCGCGGCACCGGCGGCCCACTGA

Upstream 100 bases:

>100_bases
ATCACCGCCAGAATACATGATGCAACGCATTGAGCGGTCGGAACGCCTCTGCTAGCTAGGCCGCAACCCGGAACAGACGG
GACTTTGTGGAGGACGCCCG

Downstream 100 bases:

>100_bases
GGCGGGTTCGACCGGCCAGCCTTAGACCGGCCGCCCGCGGGCGGCCGGTCGCGTTTCGGGCGCCGCGACGGACCCCGCGA
TCCGTGCTACAGGCCGCGTG

Product: L-lactate transport

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MQTWNQVYDPFGSPWLSTLAASVPVIALLAMIASGRVKAHIAAVIALGLAMLVAIVGFGMPTDLATRAAILGMVTGFFPI
GWIILNVIFLYRLTVEKGWFAILQQSVAGITADRRLQLLLVAFAFGAFFEGAGGFGTPVAVTGAILIGLGFSPLAASGLS
LIANTAPVAFGALGAPIQGLASVTGYPPEILGAMIGRQLPLFSLIVPFWLIWVFAGFRGMIRVWPPILVCGASFAVAQFL
ISNYVNPWIVDIGASLASMLALVLFLKVWQPKEIWSSPALRGHDPSLAVAGPRPVALGAGVPGTPPVHVGARTASQGEIL
MAWVPWIVLSVIVAIWGTGWFKGIVNPLFTWKYEVPGLHNVIMKVPPVVAKPVPEGAVFLFTYMSYTGTGVLIAAIISGL
IMRFSPLRLVTAYFETIWALRYSLITIAAMLALGVLTRYAGVDATLGLAFAGTGILYPFFGTLLGWLGVALTGSDTASNV
LFGGLQRITSEQLGLSGVLMAAANSSGGVMGKMVDAQSIVVASTATGYFGQEGKILRFVFWHSIALACLVGLFVMLQAYV
PFFQQMVIDLPAAAPAAH

Sequences:

>Translated_578_residues
MQTWNQVYDPFGSPWLSTLAASVPVIALLAMIASGRVKAHIAAVIALGLAMLVAIVGFGMPTDLATRAAILGMVTGFFPI
GWIILNVIFLYRLTVEKGWFAILQQSVAGITADRRLQLLLVAFAFGAFFEGAGGFGTPVAVTGAILIGLGFSPLAASGLS
LIANTAPVAFGALGAPIQGLASVTGYPPEILGAMIGRQLPLFSLIVPFWLIWVFAGFRGMIRVWPPILVCGASFAVAQFL
ISNYVNPWIVDIGASLASMLALVLFLKVWQPKEIWSSPALRGHDPSLAVAGPRPVALGAGVPGTPPVHVGARTASQGEIL
MAWVPWIVLSVIVAIWGTGWFKGIVNPLFTWKYEVPGLHNVIMKVPPVVAKPVPEGAVFLFTYMSYTGTGVLIAAIISGL
IMRFSPLRLVTAYFETIWALRYSLITIAAMLALGVLTRYAGVDATLGLAFAGTGILYPFFGTLLGWLGVALTGSDTASNV
LFGGLQRITSEQLGLSGVLMAAANSSGGVMGKMVDAQSIVVASTATGYFGQEGKILRFVFWHSIALACLVGLFVMLQAYV
PFFQQMVIDLPAAAPAAH
>Mature_578_residues
MQTWNQVYDPFGSPWLSTLAASVPVIALLAMIASGRVKAHIAAVIALGLAMLVAIVGFGMPTDLATRAAILGMVTGFFPI
GWIILNVIFLYRLTVEKGWFAILQQSVAGITADRRLQLLLVAFAFGAFFEGAGGFGTPVAVTGAILIGLGFSPLAASGLS
LIANTAPVAFGALGAPIQGLASVTGYPPEILGAMIGRQLPLFSLIVPFWLIWVFAGFRGMIRVWPPILVCGASFAVAQFL
ISNYVNPWIVDIGASLASMLALVLFLKVWQPKEIWSSPALRGHDPSLAVAGPRPVALGAGVPGTPPVHVGARTASQGEIL
MAWVPWIVLSVIVAIWGTGWFKGIVNPLFTWKYEVPGLHNVIMKVPPVVAKPVPEGAVFLFTYMSYTGTGVLIAAIISGL
IMRFSPLRLVTAYFETIWALRYSLITIAAMLALGVLTRYAGVDATLGLAFAGTGILYPFFGTLLGWLGVALTGSDTASNV
LFGGLQRITSEQLGLSGVLMAAANSSGGVMGKMVDAQSIVVASTATGYFGQEGKILRFVFWHSIALACLVGLFVMLQAYV
PFFQQMVIDLPAAAPAAH

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=565, Percent_Identity=46.1946902654867, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1790031, Length=565, Percent_Identity=41.5929203539823, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 60966; Mature: 60966

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]