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The map label for this gene is miaB

Identifier: 170747073

GI number: 170747073

Start: 686542

End: 687879

Strand: Reverse

Name: miaB

Synonym: Mrad2831_0639

Alternate gene names: 170747073

Gene position: 687879-686542 (Counterclockwise)

Preceding gene: 170747074

Following gene: 170747072

Centisome position: 11.32

GC content: 71.97

Gene sequence:

>1338_bases
TTGAAGAAGGCTTTCGTCAAATCCTACGGTTGCCAGATGAACGCCTACGACGCGGCCCGCATGGCCGACGTCCTCGGCGC
TGAGGGCTACACGGCGACCGACACGGTCGAGGACGCGGACGTCGTCGTCCTCAACACCTGCCACATCCGCGAGAAGGCCG
CCGAGAAGGTCTATTCCGAGCTCGGCCGCCTGCGCGTGCTCAAGGGCGAGCGGAAAGCGCAGGGCCTCGACACGCGCATC
GTGGTGGCCGGCTGCGTCGCCCAGGCCGAGGGCGCCGAGATCCAGGCCCGCCAGCCCGCCGTCGACGTGGTGGTCGGCCC
CCAGAGCTACCACCGCCTGCCGGACCTGCTGGCCCGCTCCCGCGAGCGCCGCGTCGTCGACACCGAGTTCCCGGTCGAGG
ACAAGTTCGACCACCTGCCCCGCCGGCGGACCCTCGGGGCGTCGGCCTTCCTCACCGTGCAGGAGGGCTGCGACAAGTTC
TGCGCCTTCTGCGTGGTCCCCTACACGCGGGGCGCGGAGGTCTCGCGCTCCGTGGCGGCGGTGCTCGCCGAGGCCGAGAA
GCTCGCCGACGGGGGCGTCCGCGAGATCACGCTCATCGGCCAGAACGTCAACGCCTATCACGGCGACGGCCCCGACGGCG
CGCCCGCGGGACTCGCCGATCTGGTGCGCGCGGTGGCGAAGATTCCCGGCATCGCGCGGATCCGCTACACGACGAGCCAC
CCGAACGATTTCGACGACGCCCTGATCCGGGCCCACGCGGAGGTGCCGGCGCTGATGCCCTACCTGCACCTGCCGGTGCA
GTCGGGCTCCGACCGGATCCTCGCCGCGATGAACCGCAAGCACACCGGCGACGCGTACCGCCGGCTGATCGACCGGATCC
GGGCCGCCCGGCCCGACATCGCCCTCTCGTCCGACTTCATCGTCGGCTTCCCGGGCGAGACCGACGCGGACTTCGCCGAG
ACCCTGCGGCTCGTGCGCGACGTCGGGTTCGAGAGCGCCTTCTCGTTCAAGTACAGCACGCGCCCCGGCACGCCGGCCGC
GGACCGCACCGATCAGGTGCCGGAGGCCGTCATGGCGGCGCGGCTCGCCGAGCTGCAGGCGCTCCTCGACAGCCAGCGCC
ACGCCTATCAGCGGGCGGCCGCGGGCCGGGTCTTCGACGTGCTGGTGGAGAAGCGCGGGCGCCATCCCGGCCAGGTCGCG
GGCAAGACGCCGCACCTGCTGGCCGTCCAGTTCGACGCCGCGCCTCACCATATCGGGCAGGTCGTGCCGGTGCGGATCAC
CGAGGCGGGGTCCAACAGCCTGTTCGGGGAACTCGTCTCCGAGGCTGCGGCGGCGTGA

Upstream 100 bases:

>100_bases
TTCGGGCGCCGCGACGGACCCCGCGATCCGTGCTACAGGCCGCGTGCCGGGCCCCGCGGGGCGGGTGCAAGGATCATCGG
ACGGTTCGGGACGGCGCGCG

Downstream 100 bases:

>100_bases
GGATCGGAGCGGTGGGGATGATGGCGAGGATGACGGCTTGAGCGCATCGGACGGTGCGGGCGCGCGCGGCGCGCTCCGGG
GTCGCGGCCCGGCGGCGCGG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKKAFVKSYGCQMNAYDAARMADVLGAEGYTATDTVEDADVVVLNTCHIREKAAEKVYSELGRLRVLKGERKAQGLDTRI
VVAGCVAQAEGAEIQARQPAVDVVVGPQSYHRLPDLLARSRERRVVDTEFPVEDKFDHLPRRRTLGASAFLTVQEGCDKF
CAFCVVPYTRGAEVSRSVAAVLAEAEKLADGGVREITLIGQNVNAYHGDGPDGAPAGLADLVRAVAKIPGIARIRYTTSH
PNDFDDALIRAHAEVPALMPYLHLPVQSGSDRILAAMNRKHTGDAYRRLIDRIRAARPDIALSSDFIVGFPGETDADFAE
TLRLVRDVGFESAFSFKYSTRPGTPAADRTDQVPEAVMAARLAELQALLDSQRHAYQRAAAGRVFDVLVEKRGRHPGQVA
GKTPHLLAVQFDAAPHHIGQVVPVRITEAGSNSLFGELVSEAAAA

Sequences:

>Translated_445_residues
MKKAFVKSYGCQMNAYDAARMADVLGAEGYTATDTVEDADVVVLNTCHIREKAAEKVYSELGRLRVLKGERKAQGLDTRI
VVAGCVAQAEGAEIQARQPAVDVVVGPQSYHRLPDLLARSRERRVVDTEFPVEDKFDHLPRRRTLGASAFLTVQEGCDKF
CAFCVVPYTRGAEVSRSVAAVLAEAEKLADGGVREITLIGQNVNAYHGDGPDGAPAGLADLVRAVAKIPGIARIRYTTSH
PNDFDDALIRAHAEVPALMPYLHLPVQSGSDRILAAMNRKHTGDAYRRLIDRIRAARPDIALSSDFIVGFPGETDADFAE
TLRLVRDVGFESAFSFKYSTRPGTPAADRTDQVPEAVMAARLAELQALLDSQRHAYQRAAAGRVFDVLVEKRGRHPGQVA
GKTPHLLAVQFDAAPHHIGQVVPVRITEAGSNSLFGELVSEAAAA
>Mature_445_residues
MKKAFVKSYGCQMNAYDAARMADVLGAEGYTATDTVEDADVVVLNTCHIREKAAEKVYSELGRLRVLKGERKAQGLDTRI
VVAGCVAQAEGAEIQARQPAVDVVVGPQSYHRLPDLLARSRERRVVDTEFPVEDKFDHLPRRRTLGASAFLTVQEGCDKF
CAFCVVPYTRGAEVSRSVAAVLAEAEKLADGGVREITLIGQNVNAYHGDGPDGAPAGLADLVRAVAKIPGIARIRYTTSH
PNDFDDALIRAHAEVPALMPYLHLPVQSGSDRILAAMNRKHTGDAYRRLIDRIRAARPDIALSSDFIVGFPGETDADFAE
TLRLVRDVGFESAFSFKYSTRPGTPAADRTDQVPEAVMAARLAELQALLDSQRHAYQRAAAGRVFDVLVEKRGRHPGQVA
GKTPHLLAVQFDAAPHHIGQVVPVRITEAGSNSLFGELVSEAAAA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=476, Percent_Identity=34.8739495798319, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI28872782, Length=476, Percent_Identity=34.8739495798319, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI93277076, Length=461, Percent_Identity=29.2841648590022, Blast_Score=183, Evalue=3e-46,
Organism=Escherichia coli, GI1786882, Length=442, Percent_Identity=50, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1787057, Length=402, Percent_Identity=30.3482587064677, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17553146, Length=471, Percent_Identity=33.3333333333333, Blast_Score=244, Evalue=8e-65,
Organism=Caenorhabditis elegans, GI71996771, Length=366, Percent_Identity=27.8688524590164, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI21356207, Length=474, Percent_Identity=32.2784810126582, Blast_Score=258, Evalue=8e-69,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=28.322440087146, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_METRJ (B1LWE8)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001753333.1
- ProteinModelPortal:   B1LWE8
- SMR:   B1LWE8
- GeneID:   6136653
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_0639
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14325
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48170; Mature: 48170

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAFVKSYGCQMNAYDAARMADVLGAEGYTATDTVEDADVVVLNTCHIREKAAEKVYSE
CCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHH
LGRLRVLKGERKAQGLDTRIVVAGCVAQAEGAEIQARQPAVDVVVGPQSYHRLPDLLARS
HHHEEEECCCHHHCCCCCEEEEEEHHHCCCCCCEECCCCCEEEEECCHHHHHHHHHHHHH
RERRVVDTEFPVEDKFDHLPRRRTLGASAFLTVQEGCDKFCAFCVVPYTRGAEVSRSVAA
HCCCEECCCCCCCCHHHHCCCHHHCCCHHEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHH
VLAEAEKLADGGVREITLIGQNVNAYHGDGPDGAPAGLADLVRAVAKIPGIARIRYTTSH
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCC
PNDFDDALIRAHAEVPALMPYLHLPVQSGSDRILAAMNRKHTGDAYRRLIDRIRAARPDI
CCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
ALSSDFIVGFPGETDADFAETLRLVRDVGFESAFSFKYSTRPGTPAADRTDQVPEAVMAA
EECCCEEEECCCCCCHHHHHHHHHHHHCCCCHHEEEEECCCCCCCCCCCCHHHHHHHHHH
RLAELQALLDSQRHAYQRAAAGRVFDVLVEKRGRHPGQVAGKTPHLLAVQFDAAPHHIGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHCC
VVPVRITEAGSNSLFGELVSEAAAA
EEEEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKAFVKSYGCQMNAYDAARMADVLGAEGYTATDTVEDADVVVLNTCHIREKAAEKVYSE
CCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHH
LGRLRVLKGERKAQGLDTRIVVAGCVAQAEGAEIQARQPAVDVVVGPQSYHRLPDLLARS
HHHEEEECCCHHHCCCCCEEEEEEHHHCCCCCCEECCCCCEEEEECCHHHHHHHHHHHHH
RERRVVDTEFPVEDKFDHLPRRRTLGASAFLTVQEGCDKFCAFCVVPYTRGAEVSRSVAA
HCCCEECCCCCCCCHHHHCCCHHHCCCHHEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHH
VLAEAEKLADGGVREITLIGQNVNAYHGDGPDGAPAGLADLVRAVAKIPGIARIRYTTSH
HHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCC
PNDFDDALIRAHAEVPALMPYLHLPVQSGSDRILAAMNRKHTGDAYRRLIDRIRAARPDI
CCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
ALSSDFIVGFPGETDADFAETLRLVRDVGFESAFSFKYSTRPGTPAADRTDQVPEAVMAA
EECCCEEEECCCCCCHHHHHHHHHHHHCCCCHHEEEEECCCCCCCCCCCCHHHHHHHHHH
RLAELQALLDSQRHAYQRAAAGRVFDVLVEKRGRHPGQVAGKTPHLLAVQFDAAPHHIGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHCC
VVPVRITEAGSNSLFGELVSEAAAA
EEEEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA