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The map label for this gene is dme [H]

Identifier: 170746932

GI number: 170746932

Start: 536226

End: 538553

Strand: Reverse

Name: dme [H]

Synonym: Mrad2831_0498

Alternate gene names: 170746932

Gene position: 538553-536226 (Counterclockwise)

Preceding gene: 170746935

Following gene: 170746931

Centisome position: 8.86

GC content: 69.59

Gene sequence:

>2328_bases
ATGGCCGATAACATGTCCGAGGACCTCAAGGCCGGGGCGCTCGTCTACCACCGCCTGCCCAAGCCCGGGAAGCTGGAGAT
CCAGGCGACCAAGCCGCTCGGCAACCAGCGCGACCTCGCCCTGGCCTACTCGCCCGGCGTCGCCGCCGCCTGCATGGCGA
TCCACGACGACCCGCAGCAGGCCGCCGAGCTCACGATCCGCCAGAACCTCGTGGCGGTGCTCTCCAACGGCACGGCCGTG
CTGGGCCTGGGCGATATCGGCCCGCTGGCCTCGAAGCCCGTGATGGAGGGCAAGGCCGTCCTGTTCAAGAAATTCGCCGG
CATCGACGTCTTCGACATCGAGGTCGACCAGAAGGACGTGTCCAAGCTCGTCGACGTGGTCTGCGCCCTCGAGCCGACCT
TCGGCGGCATCAACCTGGAGGACATCAAGGCGCCCGAGTGCTTCGAGGTCGAGGAGCAGTGCCGGGCCCGGATGAACATC
CCGGTCTTCCACGACGATCAGCACGGCACAGCGATCATCGTGGCGGCGGCCGTCCTCAACGGCCTGGAACTCGCCGGCAA
GAACCTGTCCGACGTCCGGATCGTCACGTCCGGTGCCGGCGCGGCGGCCCTCGCCTGCCTCAACCTGCTCGTGTCGCTCG
GCGCGACCCGCGAGAACATCACGGTCACCGACATCAAGGGCGTCGTCTACAAGGGCCGCCCCGAGCTGATGGACCGCTGG
AAGGACGTCTACGCGCAGGAGACCGACAAGCGGACGCTCGCCGAGGTGATCCCGGGAGCGGACGTGTTCATCGGCCTGTC
GGCCGGCGGCGTGCTCAAGCCCGAGTACCTGAAGGAGATGGCCGAGAAGCCGCTGATCATGGCGCTCGCCAACCCCTATC
CGGAGATCATGCCGGACCTGGCCGAGAAGGAGCGGCCGGACGCGATGATCTGCACCGGCCGGTCGGACTTCCCGAACCAG
GTCAACAACGTCCTGTGCTTCCCCTACATCTTCCGCGGCGCGCTCGATGTCGGCGCCCACAAGATCAACGAGGAGATGAA
GAAGGCGGCCGTGAAGGCGATCGCGGGCCTGGCCCACGAGACGACCTCCGACGTGGTCGCCCGCGCCTACGGCGGCGAGG
CCCGGCCGTTCGGGCCGAAATCCCTGATCCCGAGCCCGTTCGACCCGCGCCTGATCCTGCGCATCGCCCCGGCCGTGGCC
AAGGCCGCGATGGATTCGGGCGTCGCCGGCCGGCCGCTCCCGGATCTCGACGCCTACGTCGAGAGCCTCGACCGGTTCGT
GCACCGGTCCGGCCTGATCATGAAGCCCCTGTTCTCGAAGGCGAAGGCCGAGCCCAAGCGCGTGATCTACGCCGAGGGCG
AGGACGAGCGCGTGCTCCGCGCCGTCCAGGCGATCGTCGAGGACAAGGTCGCCTTCCCGATCCTGGTCGGCCGGCCGCGG
GTCGTGGAGACCCGGCTGAAGCGCTTCGGCCTGTCGATCGAGCACGGCCGCGACTTCGAGCTGATCGATCCCGAGGACGA
TCCCCGCTACCGCGCCTACGTGCAGACCTATCTGGAGGTGGCGGGTCGCCGGGGCATCACCCCCGACGCGGCGCGCACCC
TGGTGCGCACCAACAGCACGGTGATCGGCGCCATCGCGGTCCGCCGCGGCGAGGCGGACGCGCTGATCTGCGGCCTCGAG
GGCCGGTTCGAGACGCGGCTGCGCATCATCCGCGACGTGATCGGGCTCGCCCCCGGCGTGGAGCAGTGCGCCGCGATGAG
CCTCGTCGTGACGCATAACGGCGCCTACTTCCTGGCCGACACCCACGTCCGCCAGAACCCGACCGCCGAGGAGATCGCCG
ACGTGGCGCAGGCCTGCGCCAGCCATGTCAGCCGCTTCGGGATGACGCCGAAGATCGCGCTGCTCAGCCACTCGGATTTT
GGCCAGTCGGATTCGCCCTCCGCGATGAAGATGCGCGACGCCCTCGCGCTCCTGCACGCGCGGGATCCCGAGCTGCAGGT
CGACGGCGAGATGCAGGCGGATTCGGCCCTCAACGAGATGGTGCGCGAGCGCGTGCTGCCCGGCTCCCGCCTCAAGGGCG
CGGCCAACGTCCTGATCTTCCCGAACCTCGACGCGGCCAACATCGCCTTCCAGTTCGCGAAGGTGCTGGCGGACGCCCTG
CCGGTCGGCCCACTCCTGATCGGTCCGGCCAAGCCCGCCCACATCCTGACGCCGTCGGTGACGGCGCGCGGCATCGTCAA
CGTCACGGCCGCCGCCGTGGTCGAGGCCCAGGCGGACGAGGCCGCCCCGCGAGCCGAGGAGCGCGGTGTCGGCATGATCT
CGGAATGA

Upstream 100 bases:

>100_bases
GCCCCCTGCGCAGCCGGTGCAACAGACCGGTCATGACGCGAGCCTGCGCGATGCGCGGCCGCGTCGTCCTCGGTGAAGAA
GGTGAGCGGGAGCGTGGGCG

Downstream 100 bases:

>100_bases
GGCGTGCCCGTCGGCGGCCGCGAGCGTGGCCGTCGATTTGCTTTCCGGTCCCGGTGCCACGAAATCACCGGGAGACGACG
CGATGTGCGCGGGCGACGAT

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 775; Mature: 774

Protein sequence:

>775_residues
MADNMSEDLKAGALVYHRLPKPGKLEIQATKPLGNQRDLALAYSPGVAAACMAIHDDPQQAAELTIRQNLVAVLSNGTAV
LGLGDIGPLASKPVMEGKAVLFKKFAGIDVFDIEVDQKDVSKLVDVVCALEPTFGGINLEDIKAPECFEVEEQCRARMNI
PVFHDDQHGTAIIVAAAVLNGLELAGKNLSDVRIVTSGAGAAALACLNLLVSLGATRENITVTDIKGVVYKGRPELMDRW
KDVYAQETDKRTLAEVIPGADVFIGLSAGGVLKPEYLKEMAEKPLIMALANPYPEIMPDLAEKERPDAMICTGRSDFPNQ
VNNVLCFPYIFRGALDVGAHKINEEMKKAAVKAIAGLAHETTSDVVARAYGGEARPFGPKSLIPSPFDPRLILRIAPAVA
KAAMDSGVAGRPLPDLDAYVESLDRFVHRSGLIMKPLFSKAKAEPKRVIYAEGEDERVLRAVQAIVEDKVAFPILVGRPR
VVETRLKRFGLSIEHGRDFELIDPEDDPRYRAYVQTYLEVAGRRGITPDAARTLVRTNSTVIGAIAVRRGEADALICGLE
GRFETRLRIIRDVIGLAPGVEQCAAMSLVVTHNGAYFLADTHVRQNPTAEEIADVAQACASHVSRFGMTPKIALLSHSDF
GQSDSPSAMKMRDALALLHARDPELQVDGEMQADSALNEMVRERVLPGSRLKGAANVLIFPNLDAANIAFQFAKVLADAL
PVGPLLIGPAKPAHILTPSVTARGIVNVTAAAVVEAQADEAAPRAEERGVGMISE

Sequences:

>Translated_775_residues
MADNMSEDLKAGALVYHRLPKPGKLEIQATKPLGNQRDLALAYSPGVAAACMAIHDDPQQAAELTIRQNLVAVLSNGTAV
LGLGDIGPLASKPVMEGKAVLFKKFAGIDVFDIEVDQKDVSKLVDVVCALEPTFGGINLEDIKAPECFEVEEQCRARMNI
PVFHDDQHGTAIIVAAAVLNGLELAGKNLSDVRIVTSGAGAAALACLNLLVSLGATRENITVTDIKGVVYKGRPELMDRW
KDVYAQETDKRTLAEVIPGADVFIGLSAGGVLKPEYLKEMAEKPLIMALANPYPEIMPDLAEKERPDAMICTGRSDFPNQ
VNNVLCFPYIFRGALDVGAHKINEEMKKAAVKAIAGLAHETTSDVVARAYGGEARPFGPKSLIPSPFDPRLILRIAPAVA
KAAMDSGVAGRPLPDLDAYVESLDRFVHRSGLIMKPLFSKAKAEPKRVIYAEGEDERVLRAVQAIVEDKVAFPILVGRPR
VVETRLKRFGLSIEHGRDFELIDPEDDPRYRAYVQTYLEVAGRRGITPDAARTLVRTNSTVIGAIAVRRGEADALICGLE
GRFETRLRIIRDVIGLAPGVEQCAAMSLVVTHNGAYFLADTHVRQNPTAEEIADVAQACASHVSRFGMTPKIALLSHSDF
GQSDSPSAMKMRDALALLHARDPELQVDGEMQADSALNEMVRERVLPGSRLKGAANVLIFPNLDAANIAFQFAKVLADAL
PVGPLLIGPAKPAHILTPSVTARGIVNVTAAAVVEAQADEAAPRAEERGVGMISE
>Mature_774_residues
ADNMSEDLKAGALVYHRLPKPGKLEIQATKPLGNQRDLALAYSPGVAAACMAIHDDPQQAAELTIRQNLVAVLSNGTAVL
GLGDIGPLASKPVMEGKAVLFKKFAGIDVFDIEVDQKDVSKLVDVVCALEPTFGGINLEDIKAPECFEVEEQCRARMNIP
VFHDDQHGTAIIVAAAVLNGLELAGKNLSDVRIVTSGAGAAALACLNLLVSLGATRENITVTDIKGVVYKGRPELMDRWK
DVYAQETDKRTLAEVIPGADVFIGLSAGGVLKPEYLKEMAEKPLIMALANPYPEIMPDLAEKERPDAMICTGRSDFPNQV
NNVLCFPYIFRGALDVGAHKINEEMKKAAVKAIAGLAHETTSDVVARAYGGEARPFGPKSLIPSPFDPRLILRIAPAVAK
AAMDSGVAGRPLPDLDAYVESLDRFVHRSGLIMKPLFSKAKAEPKRVIYAEGEDERVLRAVQAIVEDKVAFPILVGRPRV
VETRLKRFGLSIEHGRDFELIDPEDDPRYRAYVQTYLEVAGRRGITPDAARTLVRTNSTVIGAIAVRRGEADALICGLEG
RFETRLRIIRDVIGLAPGVEQCAAMSLVVTHNGAYFLADTHVRQNPTAEEIADVAQACASHVSRFGMTPKIALLSHSDFG
QSDSPSAMKMRDALALLHARDPELQVDGEMQADSALNEMVRERVLPGSRLKGAANVLIFPNLDAANIAFQFAKVLADALP
VGPLLIGPAKPAHILTPSVTARGIVNVTAAAVVEAQADEAAPRAEERGVGMISE

Specific function: Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=470, Percent_Identity=26.1702127659574, Blast_Score=84, Evalue=6e-16,
Organism=Homo sapiens, GI62420880, Length=470, Percent_Identity=26.1702127659574, Blast_Score=84, Evalue=6e-16,
Organism=Homo sapiens, GI239049447, Length=470, Percent_Identity=26.1702127659574, Blast_Score=84, Evalue=6e-16,
Organism=Homo sapiens, GI4505143, Length=398, Percent_Identity=25.3768844221106, Blast_Score=80, Evalue=8e-15,
Organism=Escherichia coli, GI1788806, Length=755, Percent_Identity=58.2781456953642, Blast_Score=895, Evalue=0.0,
Organism=Escherichia coli, GI1788800, Length=322, Percent_Identity=33.8509316770186, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788635, Length=345, Percent_Identity=27.8260869565217, Blast_Score=138, Evalue=2e-33,
Organism=Escherichia coli, GI87081919, Length=292, Percent_Identity=26.3698630136986, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17537199, Length=413, Percent_Identity=25.181598062954, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322823, Length=234, Percent_Identity=27.3504273504274, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281362672, Length=396, Percent_Identity=26.7676767676768, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281362674, Length=396, Percent_Identity=26.7676767676768, Blast_Score=80, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21356279, Length=396, Percent_Identity=26.7676767676768, Blast_Score=80, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.39 [H]

Molecular weight: Translated: 83273; Mature: 83142

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNMSEDLKAGALVYHRLPKPGKLEIQATKPLGNQRDLALAYSPGVAAACMAIHDDPQQ
CCCCCCHHHHHHHHHEECCCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHCCCHHH
AAELTIRQNLVAVLSNGTAVLGLGDIGPLASKPVMEGKAVLFKKFAGIDVFDIEVDQKDV
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECHHHH
SKLVDVVCALEPTFGGINLEDIKAPECFEVEEQCRARMNIPVFHDDQHGTAIIVAAAVLN
HHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHH
GLELAGKNLSDVRIVTSGAGAAALACLNLLVSLGATRENITVTDIKGVVYKGRPELMDRW
HHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEECCEEECCCHHHHHHH
KDVYAQETDKRTLAEVIPGADVFIGLSAGGVLKPEYLKEMAEKPLIMALANPYPEIMPDL
HHHHHCCCCHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHH
AEKERPDAMICTGRSDFPNQVNNVLCFPYIFRGALDVGAHKINEEMKKAAVKAIAGLAHE
HHHCCCCEEEEECCCCCCHHHHCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTSDVVARAYGGEARPFGPKSLIPSPFDPRLILRIAPAVAKAAMDSGVAGRPLPDLDAYV
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHH
ESLDRFVHRSGLIMKPLFSKAKAEPKRVIYAEGEDERVLRAVQAIVEDKVAFPILVGRPR
HHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCH
VVETRLKRFGLSIEHGRDFELIDPEDDPRYRAYVQTYLEVAGRRGITPDAARTLVRTNST
HHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCE
VIGAIAVRRGEADALICGLEGRFETRLRIIRDVIGLAPGVEQCAAMSLVVTHNGAYFLAD
EEEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHEEEEEEECCEEEEEE
THVRQNPTAEEIADVAQACASHVSRFGMTPKIALLSHSDFGQSDSPSAMKMRDALALLHA
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHC
RDPELQVDGEMQADSALNEMVRERVLPGSRLKGAANVLIFPNLDAANIAFQFAKVLADAL
CCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHC
PVGPLLIGPAKPAHILTPSVTARGIVNVTAAAVVEAQADEAAPRAEERGVGMISE
CCCCEEECCCCCCEEECCCCHHCCHHHHHHHHHHHHCCHHCCCCHHHCCCCCCCC
>Mature Secondary Structure 
ADNMSEDLKAGALVYHRLPKPGKLEIQATKPLGNQRDLALAYSPGVAAACMAIHDDPQQ
CCCCCHHHHHHHHHEECCCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHCCCHHH
AAELTIRQNLVAVLSNGTAVLGLGDIGPLASKPVMEGKAVLFKKFAGIDVFDIEVDQKDV
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECHHHH
SKLVDVVCALEPTFGGINLEDIKAPECFEVEEQCRARMNIPVFHDDQHGTAIIVAAAVLN
HHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHH
GLELAGKNLSDVRIVTSGAGAAALACLNLLVSLGATRENITVTDIKGVVYKGRPELMDRW
HHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEECCEEECCCHHHHHHH
KDVYAQETDKRTLAEVIPGADVFIGLSAGGVLKPEYLKEMAEKPLIMALANPYPEIMPDL
HHHHHCCCCHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHH
AEKERPDAMICTGRSDFPNQVNNVLCFPYIFRGALDVGAHKINEEMKKAAVKAIAGLAHE
HHHCCCCEEEEECCCCCCHHHHCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTSDVVARAYGGEARPFGPKSLIPSPFDPRLILRIAPAVAKAAMDSGVAGRPLPDLDAYV
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHH
ESLDRFVHRSGLIMKPLFSKAKAEPKRVIYAEGEDERVLRAVQAIVEDKVAFPILVGRPR
HHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCH
VVETRLKRFGLSIEHGRDFELIDPEDDPRYRAYVQTYLEVAGRRGITPDAARTLVRTNST
HHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCE
VIGAIAVRRGEADALICGLEGRFETRLRIIRDVIGLAPGVEQCAAMSLVVTHNGAYFLAD
EEEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHEEEEEEECCEEEEEE
THVRQNPTAEEIADVAQACASHVSRFGMTPKIALLSHSDFGQSDSPSAMKMRDALALLHA
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHC
RDPELQVDGEMQADSALNEMVRERVLPGSRLKGAANVLIFPNLDAANIAFQFAKVLADAL
CCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHC
PVGPLLIGPAKPAHILTPSVTARGIVNVTAAAVVEAQADEAAPRAEERGVGMISE
CCCCEEECCCCCCEEECCCCHHCCHHHHHHHHHHHHCCHHCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]