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The map label for this gene is amdA [H]

Identifier: 170746931

GI number: 170746931

Start: 534698

End: 536143

Strand: Reverse

Name: amdA [H]

Synonym: Mrad2831_0497

Alternate gene names: 170746931

Gene position: 536143-534698 (Counterclockwise)

Preceding gene: 170746932

Following gene: 170746925

Centisome position: 8.82

GC content: 69.02

Gene sequence:

>1446_bases
ATGTGCGCGGGCGACGATCCAACCTGCCACGCCTTCGAGGAGCACCGCCGCCGGTTCCGGCGGGATCTGGAGCCTGAGCG
GCGCGCCTTCCTGAAGAGCGGCTTCGCGGCGACCGGCGCCGCCGCTGCGGCCCTCGCGGCCGGCGGCCCGTCGCTGGTGA
GCCCGGCCCTGGCCCAGGCGAGCGGGGCCAAGATCGCCGCCACGGCGCATTACCACCTGCCGGCGAATGCCGAGACCGTC
CACTGGGGCTTCTTCAGCCGCAGCCTCAAGCCCAAGGTCGAGATCGGTTCGGGCGATTTCGTCACGATCGAGACGCTCAC
GCATCAGGCGAGCGACGACGCCGAGCGGATGATCCAGGGCGATCCGGGCGCCGAGAGTGTCTATCTCTGGACGAAGGACA
AGATGGGCGTCTCGCCCCGCGGTGCCGGCGCCATGGACGCCAAGATCGGCCCCGGCGGCGGCCTCGGCGTGCATATCTGC
ACCGGCCCGGTGGCGATCCGCGGCGCCGAGCCCGGCGATGTCCTGGAGGTGCGCATCCTCGACGTCGCTCCCCGCCCCTG
CGCCAATCCCAAGTACAAGGGACTGGCCTTCGGCAGCAACGCGGCGGCGTGGTGGGGCTACCACTACAACGACCTGATCA
CCGGCGATAAGCCCCGGGAGGTCGTGACCATCTACGAGGTCGACGCCACCGGCGAGCGCGACTGGGCCCGGGCGGTCTAC
AGTTTCCGCTGGCCGGGCGTCACCGACCCGTTCGGCGTCTCTCACCCGACCATCGACTATCCCGGCCTGCCGGTCGATCA
TTCCAAGACGCAGAAGACCTTCGAGGTCCTCAAGGGCATCCGTGTGCCGATCCGGCCGCATTTCGGCACGATGGGCGTCG
CGCCCGCCGAGGCCGACCGGGTCAACACGATCCCGCCGAGCTACACCGGCGGCAACATCGACAACTGGCGGATCGGCAAG
GGCGCGACGCTGTACTACCCGGTGGCGGTGCCGGGCGCCCTGTTCTCGGTGGGCGACCCCCACGCCAGCCAGGGCGATTC
CGAATTGTGCGGCACCGCGATCGAGTGCTCGCTCACCGGAACCTTCCAGTTCATCCTGCACAAGAAGGCCGACCTGCCCG
GCACGCCGCTGGAGGCCCTCGACTTCCCGATGATCGAGACCCGGGACGACTGGGTGCTGTCCGGCTTCAGCTACCCGAAC
TACCTGCGCGACCTCGGTCCGGACGCCCAGAGCGCGATCTTCCAGAAGTCCTCCATCGACCTCGCCATGCGCGACGCCTT
CCGGAAGATGCGCCAGTTCCTGATGCACGCGAAGGGCCTCACCGAGGACGAGGCCATCTCGCTGATGTCGGTGGCGGTCG
ATTTCGGGATCACGCAGGTCGTGGACGGCAACTGGGGCGTCCACGCCGTGGTCAGGAAGGCGATCTTCTCGGATCGCGTC
GCCTGA

Upstream 100 bases:

>100_bases
GGCATGATCTCGGAATGAGGCGTGCCCGTCGGCGGCCGCGAGCGTGGCCGTCGATTTGCTTTCCGGTCCCGGTGCCACGA
AATCACCGGGAGACGACGCG

Downstream 100 bases:

>100_bases
CGTCGGGCCGGATCACGCGGCCTTCGCGGTTCCGGCCATCGTCATCAGGCCGGTGATGACCCGGTAGGTCTCGACCCAGG
CCTCCCGGAGTTCCGGCGTG

Product: acetamidase/formamidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MCAGDDPTCHAFEEHRRRFRRDLEPERRAFLKSGFAATGAAAAALAAGGPSLVSPALAQASGAKIAATAHYHLPANAETV
HWGFFSRSLKPKVEIGSGDFVTIETLTHQASDDAERMIQGDPGAESVYLWTKDKMGVSPRGAGAMDAKIGPGGGLGVHIC
TGPVAIRGAEPGDVLEVRILDVAPRPCANPKYKGLAFGSNAAAWWGYHYNDLITGDKPREVVTIYEVDATGERDWARAVY
SFRWPGVTDPFGVSHPTIDYPGLPVDHSKTQKTFEVLKGIRVPIRPHFGTMGVAPAEADRVNTIPPSYTGGNIDNWRIGK
GATLYYPVAVPGALFSVGDPHASQGDSELCGTAIECSLTGTFQFILHKKADLPGTPLEALDFPMIETRDDWVLSGFSYPN
YLRDLGPDAQSAIFQKSSIDLAMRDAFRKMRQFLMHAKGLTEDEAISLMSVAVDFGITQVVDGNWGVHAVVRKAIFSDRV
A

Sequences:

>Translated_481_residues
MCAGDDPTCHAFEEHRRRFRRDLEPERRAFLKSGFAATGAAAAALAAGGPSLVSPALAQASGAKIAATAHYHLPANAETV
HWGFFSRSLKPKVEIGSGDFVTIETLTHQASDDAERMIQGDPGAESVYLWTKDKMGVSPRGAGAMDAKIGPGGGLGVHIC
TGPVAIRGAEPGDVLEVRILDVAPRPCANPKYKGLAFGSNAAAWWGYHYNDLITGDKPREVVTIYEVDATGERDWARAVY
SFRWPGVTDPFGVSHPTIDYPGLPVDHSKTQKTFEVLKGIRVPIRPHFGTMGVAPAEADRVNTIPPSYTGGNIDNWRIGK
GATLYYPVAVPGALFSVGDPHASQGDSELCGTAIECSLTGTFQFILHKKADLPGTPLEALDFPMIETRDDWVLSGFSYPN
YLRDLGPDAQSAIFQKSSIDLAMRDAFRKMRQFLMHAKGLTEDEAISLMSVAVDFGITQVVDGNWGVHAVVRKAIFSDRV
A
>Mature_481_residues
MCAGDDPTCHAFEEHRRRFRRDLEPERRAFLKSGFAATGAAAAALAAGGPSLVSPALAQASGAKIAATAHYHLPANAETV
HWGFFSRSLKPKVEIGSGDFVTIETLTHQASDDAERMIQGDPGAESVYLWTKDKMGVSPRGAGAMDAKIGPGGGLGVHIC
TGPVAIRGAEPGDVLEVRILDVAPRPCANPKYKGLAFGSNAAAWWGYHYNDLITGDKPREVVTIYEVDATGERDWARAVY
SFRWPGVTDPFGVSHPTIDYPGLPVDHSKTQKTFEVLKGIRVPIRPHFGTMGVAPAEADRVNTIPPSYTGGNIDNWRIGK
GATLYYPVAVPGALFSVGDPHASQGDSELCGTAIECSLTGTFQFILHKKADLPGTPLEALDFPMIETRDDWVLSGFSYPN
YLRDLGPDAQSAIFQKSSIDLAMRDAFRKMRQFLMHAKGLTEDEAISLMSVAVDFGITQVVDGNWGVHAVVRKAIFSDRV
A

Specific function: Allows acetamide to be used as a sole carbon or nitrogen source [H]

COG id: COG2421

COG function: function code C; Predicted acetamidase/formamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetamidase/formamidase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004304 [H]

Pfam domain/function: PF03069 FmdA_AmdA [H]

EC number: NA

Molecular weight: Translated: 51838; Mature: 51838

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAGDDPTCHAFEEHRRRFRRDLEPERRAFLKSGFAATGAAAAALAAGGPSLVSPALAQA
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHC
SGAKIAATAHYHLPANAETVHWGFFSRSLKPKVEIGSGDFVTIETLTHQASDDAERMIQG
CCCEEEEEEEEECCCCCCEEEEEHHCCCCCCEEEECCCCEEEEEEECCCCCCCHHHHHCC
DPGAESVYLWTKDKMGVSPRGAGAMDAKIGPGGGLGVHICTGPVAIRGAEPGDVLEVRIL
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCCCEEEEEEE
DVAPRPCANPKYKGLAFGSNAAAWWGYHYNDLITGDKPREVVTIYEVDATGERDWARAVY
EECCCCCCCCCCCCEEECCCCCEEECCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHH
SFRWPGVTDPFGVSHPTIDYPGLPVDHSKTQKTFEVLKGIRVPIRPHFGTMGVAPAEADR
EEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
VNTIPPSYTGGNIDNWRIGKGATLYYPVAVPGALFSVGDPHASQGDSELCGTAIECSLTG
CCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCEEECCCCCCCCCCHHHCCCEEEEEECC
TFQFILHKKADLPGTPLEALDFPMIETRDDWVLSGFSYPNYLRDLGPDAQSAIFQKSSID
EEEEEEEECCCCCCCCHHHHCCCCEECCCCEEEECCCCHHHHHHCCCCHHHHHHHHCCCC
LAMRDAFRKMRQFLMHAKGLTEDEAISLMSVAVDFGITQVVDGNWGVHAVVRKAIFSDRV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCC
A
C
>Mature Secondary Structure
MCAGDDPTCHAFEEHRRRFRRDLEPERRAFLKSGFAATGAAAAALAAGGPSLVSPALAQA
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHC
SGAKIAATAHYHLPANAETVHWGFFSRSLKPKVEIGSGDFVTIETLTHQASDDAERMIQG
CCCEEEEEEEEECCCCCCEEEEEHHCCCCCCEEEECCCCEEEEEEECCCCCCCHHHHHCC
DPGAESVYLWTKDKMGVSPRGAGAMDAKIGPGGGLGVHICTGPVAIRGAEPGDVLEVRIL
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCCCEEEEEEE
DVAPRPCANPKYKGLAFGSNAAAWWGYHYNDLITGDKPREVVTIYEVDATGERDWARAVY
EECCCCCCCCCCCCEEECCCCCEEECCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHH
SFRWPGVTDPFGVSHPTIDYPGLPVDHSKTQKTFEVLKGIRVPIRPHFGTMGVAPAEADR
EEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
VNTIPPSYTGGNIDNWRIGKGATLYYPVAVPGALFSVGDPHASQGDSELCGTAIECSLTG
CCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCEEECCCCCCCCCCHHHCCCEEEEEECC
TFQFILHKKADLPGTPLEALDFPMIETRDDWVLSGFSYPNYLRDLGPDAQSAIFQKSSID
EEEEEEEECCCCCCCCHHHHCCCCEECCCCEEEECCCCHHHHHHCCCCHHHHHHHHCCCC
LAMRDAFRKMRQFLMHAKGLTEDEAISLMSVAVDFGITQVVDGNWGVHAVVRKAIFSDRV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8473863 [H]