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The map label for this gene is puuB [H]

Identifier: 170746803

GI number: 170746803

Start: 391941

End: 393230

Strand: Reverse

Name: puuB [H]

Synonym: Mrad2831_0357

Alternate gene names: 170746803

Gene position: 393230-391941 (Counterclockwise)

Preceding gene: 170746804

Following gene: 170746800

Centisome position: 6.47

GC content: 73.02

Gene sequence:

>1290_bases
GTGGCCGAACCGTTCCCGCTCGCACCCTCGCTCTGGGCCACCACGGCGGCGCCCGCGCCGGACACGCCGCCCCTCGCCAG
ATCAGGCTTGGCCGAGGTGGTGATCGTCGGCGGCGGGTTCTGCGGCCTCTCCACCGCCCTGCATCTCGCCGAGCGCGGCA
TCCGACCCGTCGTGCTCGAGGCGCAGGAGGTCGGCTACGGCGGGTCCGGTCGCAACGGTGGCCAGGTCATCCCGGGCCTC
AAATACGATCCCGCCGACCTCGTCGCGCGGTTCGGTCGGGAGCGCGGCGAGCGGCTCGCGCGCTTCGCCGGCGGGACCGC
CGACGTCGTGTTCGATCTCATCGCCCGGCACGGGATGGACGTGCCGCACCGCCGCTCCGGCTGGATCCAGGGCGCCCATA
CCGAGGCCGGGCTCGCCGAGGTCGCGAAGCGGGCGGCCCAGTGGGCGGAGCTCGGGGCCCCGACCCGCGTCCTCGACCGG
GCCGAGACCGAGCAGCTGCTGGGGACCGACCGCTATCTCGGCGGCTGGCTCGACGGCCGCGGCGGGGCGATCCAGCCCCT
GAGCTACGCCCGCGGCCTCGCCCGGGCTGCCCAGAAGGCCGGCGCCATAATCCACGGCGCGTCGCCTGTCACCGCCCTCG
AACGGACCGGGTCGGGCTGGACGGTCACGACGGCCCAGGGCGAGCGGGTGACGACCGGCCGGGTCGTTCTGTGCACCAAC
GGCTACACGGGCGGGCTCTGGCCGAACCTCGCGCGGACGGTGATCGCGGCGAATTCGTTCCAGGTCGCGACGGTGCCGCT
GACGGACAACCTGCGGCGCTCGATCCTGCCCGAAGGTCACGTCTCCTCGGACACGCGGAAGCTCCTGCTCTACTTCCGCC
TCGACCATACCGGCCGCCTGCTGATGGGCGGACGCGGGCCGTTCCGGGAGCCGCGCAGCCCCGCCGACTGGGCGCATCTC
GAGCGGATCGTGGCCAAGCTCTTCCCGCAGCTGGACGGGATCGCCTTCGACCATCGCTGGTGCGGCCGGGTCGCGATCAC
CCGGGACTACCTGCCCCATCTCCACGAGCCGGCGCCCGGGCTGCTCATCGATATCGGCTGCCAGGGTCGGGGCGTCGGCC
TGCAGTCGGCGATGGGCCGGGCGATGGCCACCTACATCGCCACCGGGGATGCCGGCAGCCTGCCTTTGCCGCTCACGCCG
ATCGAGCCGCTTCCGCTTCACGGTCTCAATCGGCTGTACGTCTCGGCGATCATCGCGTGGTACCGCCTGCGTGACGGAGG
CATTCGCTGA

Upstream 100 bases:

>100_bases
TCGTCGAGCAGGGACCGGCCGCCGAAGTCCTCAACCGGCCGCAGAACCCGCGCACCCGGGACTTCCTGCAGCGCGTGCTG
CACCCGCTCTAGGACAAGCC

Downstream 100 bases:

>100_bases
TCGGTCCGGCGCGCCGTCGAACGCGCCGTCACCGCGGGTGCCACAGCGCCGCGCTGAGGCCCAGAGCGGCGGGGCCAGAG
CGACGGCGCCAGGGCGAGGA

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MAEPFPLAPSLWATTAAPAPDTPPLARSGLAEVVIVGGGFCGLSTALHLAERGIRPVVLEAQEVGYGGSGRNGGQVIPGL
KYDPADLVARFGRERGERLARFAGGTADVVFDLIARHGMDVPHRRSGWIQGAHTEAGLAEVAKRAAQWAELGAPTRVLDR
AETEQLLGTDRYLGGWLDGRGGAIQPLSYARGLARAAQKAGAIIHGASPVTALERTGSGWTVTTAQGERVTTGRVVLCTN
GYTGGLWPNLARTVIAANSFQVATVPLTDNLRRSILPEGHVSSDTRKLLLYFRLDHTGRLLMGGRGPFREPRSPADWAHL
ERIVAKLFPQLDGIAFDHRWCGRVAITRDYLPHLHEPAPGLLIDIGCQGRGVGLQSAMGRAMATYIATGDAGSLPLPLTP
IEPLPLHGLNRLYVSAIIAWYRLRDGGIR

Sequences:

>Translated_429_residues
MAEPFPLAPSLWATTAAPAPDTPPLARSGLAEVVIVGGGFCGLSTALHLAERGIRPVVLEAQEVGYGGSGRNGGQVIPGL
KYDPADLVARFGRERGERLARFAGGTADVVFDLIARHGMDVPHRRSGWIQGAHTEAGLAEVAKRAAQWAELGAPTRVLDR
AETEQLLGTDRYLGGWLDGRGGAIQPLSYARGLARAAQKAGAIIHGASPVTALERTGSGWTVTTAQGERVTTGRVVLCTN
GYTGGLWPNLARTVIAANSFQVATVPLTDNLRRSILPEGHVSSDTRKLLLYFRLDHTGRLLMGGRGPFREPRSPADWAHL
ERIVAKLFPQLDGIAFDHRWCGRVAITRDYLPHLHEPAPGLLIDIGCQGRGVGLQSAMGRAMATYIATGDAGSLPLPLTP
IEPLPLHGLNRLYVSAIIAWYRLRDGGIR
>Mature_428_residues
AEPFPLAPSLWATTAAPAPDTPPLARSGLAEVVIVGGGFCGLSTALHLAERGIRPVVLEAQEVGYGGSGRNGGQVIPGLK
YDPADLVARFGRERGERLARFAGGTADVVFDLIARHGMDVPHRRSGWIQGAHTEAGLAEVAKRAAQWAELGAPTRVLDRA
ETEQLLGTDRYLGGWLDGRGGAIQPLSYARGLARAAQKAGAIIHGASPVTALERTGSGWTVTTAQGERVTTGRVVLCTNG
YTGGLWPNLARTVIAANSFQVATVPLTDNLRRSILPEGHVSSDTRKLLLYFRLDHTGRLLMGGRGPFREPRSPADWAHLE
RIVAKLFPQLDGIAFDHRWCGRVAITRDYLPHLHEPAPGLLIDIGCQGRGVGLQSAMGRAMATYIATGDAGSLPLPLTPI
EPLPLHGLNRLYVSAIIAWYRLRDGGIR

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI24797151, Length=221, Percent_Identity=29.4117647058824, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1787559, Length=424, Percent_Identity=32.5471698113208, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 45819; Mature: 45688

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPFPLAPSLWATTAAPAPDTPPLARSGLAEVVIVGGGFCGLSTALHLAERGIRPVVLE
CCCCCCCCCCHHHCCCCCCCCCCCHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEE
AQEVGYGGSGRNGGQVIPGLKYDPADLVARFGRERGERLARFAGGTADVVFDLIARHGMD
HHHCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
VPHRRSGWIQGAHTEAGLAEVAKRAAQWAELGAPTRVLDRAETEQLLGTDRYLGGWLDGR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHCCCCCCC
GGAIQPLSYARGLARAAQKAGAIIHGASPVTALERTGSGWTVTTAQGERVTTGRVVLCTN
CCCCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHCCCCEEEEECCCCEEECCEEEEEEC
GYTGGLWPNLARTVIAANSFQVATVPLTDNLRRSILPEGHVSSDTRKLLLYFRLDHTGRL
CCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCCCCCCEEEEEEEEECCCCEE
LMGGRGPFREPRSPADWAHLERIVAKLFPQLDGIAFDHRWCGRVAITRDYLPHLHEPAPG
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCEEEEHHHHHHCCCCCCC
LLIDIGCQGRGVGLQSAMGRAMATYIATGDAGSLPLPLTPIEPLPLHGLNRLYVSAIIAW
EEEEECCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
YRLRDGGIR
HHHCCCCCC
>Mature Secondary Structure 
AEPFPLAPSLWATTAAPAPDTPPLARSGLAEVVIVGGGFCGLSTALHLAERGIRPVVLE
CCCCCCCCCHHHCCCCCCCCCCCHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEE
AQEVGYGGSGRNGGQVIPGLKYDPADLVARFGRERGERLARFAGGTADVVFDLIARHGMD
HHHCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
VPHRRSGWIQGAHTEAGLAEVAKRAAQWAELGAPTRVLDRAETEQLLGTDRYLGGWLDGR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHCCCCCCC
GGAIQPLSYARGLARAAQKAGAIIHGASPVTALERTGSGWTVTTAQGERVTTGRVVLCTN
CCCCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHCCCCEEEEECCCCEEECCEEEEEEC
GYTGGLWPNLARTVIAANSFQVATVPLTDNLRRSILPEGHVSSDTRKLLLYFRLDHTGRL
CCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCCCCCCEEEEEEEEECCCCEE
LMGGRGPFREPRSPADWAHLERIVAKLFPQLDGIAFDHRWCGRVAITRDYLPHLHEPAPG
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCEEEEHHHHHHCCCCCCC
LLIDIGCQGRGVGLQSAMGRAMATYIATGDAGSLPLPLTPIEPLPLHGLNRLYVSAIIAW
EEEEECCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
YRLRDGGIR
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]