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The map label for this gene is alkJ [H]

Identifier: 170746779

GI number: 170746779

Start: 362834

End: 364468

Strand: Reverse

Name: alkJ [H]

Synonym: Mrad2831_0332

Alternate gene names: 170746779

Gene position: 364468-362834 (Counterclockwise)

Preceding gene: 170746780

Following gene: 170746778

Centisome position: 6.0

GC content: 73.7

Gene sequence:

>1635_bases
ATGGCGCGCACCGTGGGATCGACCGACATCGACGTCGTCGCGGCGGGCGTCTTCGACTACGTGATCGTGGGCGGCGGCAC
CGCGGGCTGCGTCCTGGCCAACCGGCTGACCGAGTCCGGGACGCGCCGGGTGCTGCTCCTCGAGGCGGGGGGGCGGGCGC
GCAGCCCCTGGGTCTCGATCCCGGCCGGGTTCAGCCGCCTGCTCCAGCACCCCTCCTACAACTGGCGCTTCCGGACCGAG
CCCGAGGAGGCGACCGGCGGCCGGGTGATCGCGGTTCCGCGGGGCAAGGGCCTCGGCGGGTCGACCCTCATCAACGGCAT
GATCTACGTGCGCGGGCAGCCGCAGGACTACGACGCCTGGGCGCAGGCCGGCTGCCGCGGATGGGGCTTCGCGGACGTGC
TGCCCTACTTCCGCAGGCTCGAGGATTACGACGGGGCGCCCGACCCCCTGCGGGCCCGCGGCGGACCGCTGCCGATCACG
GAAGTCGCCGAGCGGCCGGCGATCGCCGAGGCCTTCATCGCCGCCGCGCGGGCGGCTGGGCATCCGCGCAACCCCGACTA
CAACGCGGCGAGTCAGGACGGCTTCGGCTACTACCAAGTGAACCAGCGTCGCGGGCGGCGGGTCAGCGCCGCCGACGCCT
ATCTGCGGCCGGCCCTCGCCCGTCCGAACCTCGCCGTCGTGACGGACGCGCACGTCCTGCGGCTCACCTTCGACAGGGAT
CGGGCGAGCGGCGTCGCCGCTCTGGTCGGCGGCCGCGAGCGGCTGTTCCGCTGCCGCGGGGAGGTGATCCTCGCCGCAGG
GGCGGCGCAGACCCCGCAGATCCTGGAACTGTCCGGCCTCGGCGACCCGTCGATCCTCGCCGGCCTCGGCGTGGCGGTGC
GGCGCGCGCTCCCCGCCGTCGGCGCCAACTACATGGACCATTACTGCACGCGCATGAACTGGCGCGTGCGCCTGCCGGTG
ACGCTGAACGAGCAAACCCGCGGCCTGCGCCTCGGCGTCGCCGCGGCGCGCTACCTCGCGACGCGGCAGGGTATCCTGGC
GCTGGGGACCGGCCTCGCCCACGGGTTCGTGCGCACCCGGCCGGGGCTCGACGGGCCCGACGTGCAGTACTTCTTCATGC
ACGCGAGCTACGCCAACGCCGCCGAGCGCAAGCTCGACCGCCTGCCCGGCATGACCATCGGCGTCACGCAGCTGCGACCG
GAATCGCGCGGGACGATCCACGCGGCGTCGCCCGACCCGCTCGAGCCGCCCGCCATCCGCCCGAACTTCCTCGCCACCGA
GGAGGATCGCCGGGCGATGGTCGAGGGCATGAAGATCGCGCGCACCGTCGTCGCGCAGGCGCCGATGGAGCGCTTTCGCG
GGGCCGAGCTCAATCCGGGGCCGGACTGCCGGACCGACGCGGACTGGCTCGATTTCGCCCGCCGCGACGGCCAGACGATC
TACCACATCTGCGGCACGTGCCGCATGGGCGGCGATCCGGGTTCCGTGACGGATCCCAACCTGCGTGTGCGCGGCGTGGC
CGGCCTCCGGGTCGCGGACGCCTCGATCATGCCGCGGATCGTCTCCGGCAACACGCAGGCCGCGGTGTTCATGATCGCCG
AGAAGGCCGCCGACCTGATCCGGGCCGATGCCTGA

Upstream 100 bases:

>100_bases
CGGCTGCCGCATTCAGAGGGGGCTACCGATGCTGGAGTGCCAGATCGCCCTCATGGCCCGCCACATGGGCGCCCTCTGAG
GCTGGATCCGAGGACTGAGC

Downstream 100 bases:

>100_bases
TGCGGTCTGGCCATCGGGCGCCGCGGCGCGTACGGCAGGATCGCGCCAACCTCGGCCGTACTGCGCCGGTCCCGGCCGTG
TCTCGGCGTCGACCGGCACT

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MARTVGSTDIDVVAAGVFDYVIVGGGTAGCVLANRLTESGTRRVLLLEAGGRARSPWVSIPAGFSRLLQHPSYNWRFRTE
PEEATGGRVIAVPRGKGLGGSTLINGMIYVRGQPQDYDAWAQAGCRGWGFADVLPYFRRLEDYDGAPDPLRARGGPLPIT
EVAERPAIAEAFIAAARAAGHPRNPDYNAASQDGFGYYQVNQRRGRRVSAADAYLRPALARPNLAVVTDAHVLRLTFDRD
RASGVAALVGGRERLFRCRGEVILAAGAAQTPQILELSGLGDPSILAGLGVAVRRALPAVGANYMDHYCTRMNWRVRLPV
TLNEQTRGLRLGVAAARYLATRQGILALGTGLAHGFVRTRPGLDGPDVQYFFMHASYANAAERKLDRLPGMTIGVTQLRP
ESRGTIHAASPDPLEPPAIRPNFLATEEDRRAMVEGMKIARTVVAQAPMERFRGAELNPGPDCRTDADWLDFARRDGQTI
YHICGTCRMGGDPGSVTDPNLRVRGVAGLRVADASIMPRIVSGNTQAAVFMIAEKAADLIRADA

Sequences:

>Translated_544_residues
MARTVGSTDIDVVAAGVFDYVIVGGGTAGCVLANRLTESGTRRVLLLEAGGRARSPWVSIPAGFSRLLQHPSYNWRFRTE
PEEATGGRVIAVPRGKGLGGSTLINGMIYVRGQPQDYDAWAQAGCRGWGFADVLPYFRRLEDYDGAPDPLRARGGPLPIT
EVAERPAIAEAFIAAARAAGHPRNPDYNAASQDGFGYYQVNQRRGRRVSAADAYLRPALARPNLAVVTDAHVLRLTFDRD
RASGVAALVGGRERLFRCRGEVILAAGAAQTPQILELSGLGDPSILAGLGVAVRRALPAVGANYMDHYCTRMNWRVRLPV
TLNEQTRGLRLGVAAARYLATRQGILALGTGLAHGFVRTRPGLDGPDVQYFFMHASYANAAERKLDRLPGMTIGVTQLRP
ESRGTIHAASPDPLEPPAIRPNFLATEEDRRAMVEGMKIARTVVAQAPMERFRGAELNPGPDCRTDADWLDFARRDGQTI
YHICGTCRMGGDPGSVTDPNLRVRGVAGLRVADASIMPRIVSGNTQAAVFMIAEKAADLIRADA
>Mature_543_residues
ARTVGSTDIDVVAAGVFDYVIVGGGTAGCVLANRLTESGTRRVLLLEAGGRARSPWVSIPAGFSRLLQHPSYNWRFRTEP
EEATGGRVIAVPRGKGLGGSTLINGMIYVRGQPQDYDAWAQAGCRGWGFADVLPYFRRLEDYDGAPDPLRARGGPLPITE
VAERPAIAEAFIAAARAAGHPRNPDYNAASQDGFGYYQVNQRRGRRVSAADAYLRPALARPNLAVVTDAHVLRLTFDRDR
ASGVAALVGGRERLFRCRGEVILAAGAAQTPQILELSGLGDPSILAGLGVAVRRALPAVGANYMDHYCTRMNWRVRLPVT
LNEQTRGLRLGVAAARYLATRQGILALGTGLAHGFVRTRPGLDGPDVQYFFMHASYANAAERKLDRLPGMTIGVTQLRPE
SRGTIHAASPDPLEPPAIRPNFLATEEDRRAMVEGMKIARTVVAQAPMERFRGAELNPGPDCRTDADWLDFARRDGQTIY
HICGTCRMGGDPGSVTDPNLRVRGVAGLRVADASIMPRIVSGNTQAAVFMIAEKAADLIRADA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=533, Percent_Identity=39.0243902439024, Blast_Score=362, Evalue=1e-100,
Organism=Escherichia coli, GI1786503, Length=533, Percent_Identity=39.5872420262664, Blast_Score=357, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17532301, Length=548, Percent_Identity=35.2189781021898, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24642048, Length=568, Percent_Identity=36.9718309859155, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI45551458, Length=567, Percent_Identity=36.68430335097, Blast_Score=305, Evalue=5e-83,
Organism=Drosophila melanogaster, GI45549471, Length=567, Percent_Identity=37.037037037037, Blast_Score=305, Evalue=5e-83,
Organism=Drosophila melanogaster, GI24642059, Length=567, Percent_Identity=36.331569664903, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24642055, Length=580, Percent_Identity=36.0344827586207, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24642042, Length=566, Percent_Identity=37.2791519434629, Blast_Score=295, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24650267, Length=568, Percent_Identity=35.2112676056338, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24642035, Length=573, Percent_Identity=35.0785340314136, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24642039, Length=578, Percent_Identity=35.4671280276817, Blast_Score=279, Evalue=4e-75,
Organism=Drosophila melanogaster, GI24642037, Length=579, Percent_Identity=35.2331606217617, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI17137792, Length=554, Percent_Identity=34.6570397111913, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI18859995, Length=573, Percent_Identity=35.6020942408377, Blast_Score=271, Evalue=6e-73,
Organism=Drosophila melanogaster, GI18859993, Length=570, Percent_Identity=32.280701754386, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24642051, Length=571, Percent_Identity=32.7495621716287, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI24645930, Length=582, Percent_Identity=30.0687285223368, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24642057, Length=592, Percent_Identity=27.5337837837838, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 58600; Mature: 58468

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTVGSTDIDVVAAGVFDYVIVGGGTAGCVLANRLTESGTRRVLLLEAGGRARSPWVSI
CCCCCCCCCHHHHHHHHEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEC
PAGFSRLLQHPSYNWRFRTEPEEATGGRVIAVPRGKGLGGSTLINGMIYVRGQPQDYDAW
CHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCCCCCCHHEECCEEEEECCCCCHHHH
AQAGCRGWGFADVLPYFRRLEDYDGAPDPLRARGGPLPITEVAERPAIAEAFIAAARAAG
HHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHCCCHHHHHHHHHHHHCC
HPRNPDYNAASQDGFGYYQVNQRRGRRVSAADAYLRPALARPNLAVVTDAHVLRLTFDRD
CCCCCCCCCCCCCCCEEEEECCCCCCEECHHHHHHHHHHCCCCEEEEECCEEEEEEECCC
RASGVAALVGGRERLFRCRGEVILAAGAAQTPQILELSGLGDPSILAGLGVAVRRALPAV
CCCCEEEECCCHHHHHHHCCCEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
GANYMDHYCTRMNWRVRLPVTLNEQTRGLRLGVAAARYLATRQGILALGTGLAHGFVRTR
CCHHHHHHHHCCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
PGLDGPDVQYFFMHASYANAAERKLDRLPGMTIGVTQLRPESRGTIHAASPDPLEPPAIR
CCCCCCCCEEEEEECHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCC
PNFLATEEDRRAMVEGMKIARTVVAQAPMERFRGAELNPGPDCRTDADWLDFARRDGQTI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEE
YHICGTCRMGGDPGSVTDPNLRVRGVAGLRVADASIMPRIVSGNTQAAVFMIAEKAADLI
EEEEEEEECCCCCCCCCCCCEEEEEECCEEEECHHHCCEEECCCCCEEEEEEEHHHHHHH
RADA
HCCC
>Mature Secondary Structure 
ARTVGSTDIDVVAAGVFDYVIVGGGTAGCVLANRLTESGTRRVLLLEAGGRARSPWVSI
CCCCCCCCHHHHHHHHEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEC
PAGFSRLLQHPSYNWRFRTEPEEATGGRVIAVPRGKGLGGSTLINGMIYVRGQPQDYDAW
CHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEECCCCCCCHHEECCEEEEECCCCCHHHH
AQAGCRGWGFADVLPYFRRLEDYDGAPDPLRARGGPLPITEVAERPAIAEAFIAAARAAG
HHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHCCCHHHHHHHHHHHHCC
HPRNPDYNAASQDGFGYYQVNQRRGRRVSAADAYLRPALARPNLAVVTDAHVLRLTFDRD
CCCCCCCCCCCCCCCEEEEECCCCCCEECHHHHHHHHHHCCCCEEEEECCEEEEEEECCC
RASGVAALVGGRERLFRCRGEVILAAGAAQTPQILELSGLGDPSILAGLGVAVRRALPAV
CCCCEEEECCCHHHHHHHCCCEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
GANYMDHYCTRMNWRVRLPVTLNEQTRGLRLGVAAARYLATRQGILALGTGLAHGFVRTR
CCHHHHHHHHCCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
PGLDGPDVQYFFMHASYANAAERKLDRLPGMTIGVTQLRPESRGTIHAASPDPLEPPAIR
CCCCCCCCEEEEEECHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCC
PNFLATEEDRRAMVEGMKIARTVVAQAPMERFRGAELNPGPDCRTDADWLDFARRDGQTI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEE
YHICGTCRMGGDPGSVTDPNLRVRGVAGLRVADASIMPRIVSGNTQAAVFMIAEKAADLI
EEEEEEEECCCCCCCCCCCCEEEEEECCEEEECHHHCCEEECCCCCEEEEEEEHHHHHHH
RADA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]