Click here to switch to the map view.

The map label for this gene is 170746679

Identifier: 170746679

GI number: 170746679

Start: 245289

End: 246785

Strand: Reverse

Name: 170746679

Synonym: Mrad2831_0230

Alternate gene names: NA

Gene position: 246785-245289 (Counterclockwise)

Preceding gene: 170746680

Following gene: 170746675

Centisome position: 4.06

GC content: 72.81

Gene sequence:

>1497_bases
GTGACCGACCTTTGCGACATGTCCGCGGTGGACCTGCGCCGCCTCATCGGGCGCGGGGATGTCTCCCCCGTCGAACTCAT
GGACTCGTGTCTCGACCGGATCGCCCGGATCAACCCGGCAGTGAACGCGGTCGTCGCCCTCGATACCGAGCGGGCGCGGG
CCGCGGCGCGGAGAGCCGAGGCCGCGGTCCGCGTCGGCGAGCCCCTTGGCCCGCTCCACGGCATCCCGGTCGGCATCAAG
GATACGGAGGACACGGCCGGGCTGCGCACGACCTACGGCAGCCCGATCCACGCCGACAACGTCCCGGCGCGGGACCTCGG
CGTCGTCGCGCGGCTGCGCGAGGCCGGAGCCATCGTGCTGGCCAAGACCAACACGCCCGAATTCGCGGCCGGCGCGAACA
CGCGCAACACCGTCTACGGTGCCACCGGCAACGCCTTCGATCCGACGCGCTCCGCGGCGGGTTCATCGGGTGGCTCGGCG
GTCGCCCTCGCCTGCGGCATGGTCCCGCTCGCGTCCGGCTCGGATACCGGGGGGTCGCTGCGCAACCCGGCCGCCTACGC
GGGGATCGTCGGGATGCGCCCGTCCTCCGGACTGGTCCCGAACGAGCGTCGCGGGCTCGGTTGGGCCAATCTTCCGGTGA
ACGGGCCGATGGCCCGGACGGTGGGCGATGCCGCCCTCATGCTCGGCGCGATGGCCAACGATGATCCGCGCGATCCCCTC
GCCTACACGCTGCCGGGCGAGCCGGTGCGCGGGCGCGCGGATCGCTACGACCCGCACCGCGCGCTCGACCTCGGCGCCCT
CCGCGTCGCGTTCACGGAGGATTTCGGCTTCGCGCCGACCGAGCAGCACGTCCGGCGCGTGTTCCGTCACCGGGTCGCCT
CCCTGTCGCCGCTCTTCGCCACCGCCGAGACGGCGACACCGGACTGCGCGGGGGCGGATGAGGCCTTCGCGATCCTCCGG
GCCGCGCAGTTCCTCGCGCTCCACCTCGACAATTACCGCACCCGGCCGCACCTTCTCGGACCGAATGTCCGCACGAACGT
CGAGGAAGGCCTCGGCTACACGCTCGCCGACTATGCCCGGGCGGCGAGCGTCCAGACCGCCATCTACCGGACTTTTGTGG
ACTTCTTCCGTCGGTACGACCTGCTGATCAGCCCGACCATCACGCTCTCGCCCCGCCCCTGGACCGAGCTGTACCCGACA
GAGATCGACGGTGCCGCCACCCGCTCGTACTTCCACTGGCTGGCCCTGGCCTACGGCGTGACCCTCGCAGGCCACCCGGC
GATCAGCCTGCCGCTCGGTGTCGACGAGGCCGGCCTGCCCTTCGGCGTACAGGTCGTGGCCCGGCGCGGCGGCGACGCCG
ACCTGCTCGCGGCGGCGGCGGCCATCGAGGCCCACTGCGCCGGCCGACCGGAGCTGACCCGTCCGAGACCCGACCTCGCT
GGACTGGCCGGGCGGGCGCCGATCGCCCGGAGCCCCGGCTTCTTCGATCTCGGCTGA

Upstream 100 bases:

>100_bases
CGCATTGACCATCCGGAGCCGCTCGACCGCCGCCGTGCCGCAGGCGGCCTGACGAAGTTTCCCATCGATCCGCGTGCCGA
ATTCTGACGGAGATGACGCC

Downstream 100 bases:

>100_bases
TCGATCGCGCCCCAGTGGAGCCGTCGGCACCGGCCGGCGGTCACTCCATCAGGTCGGTCAGAGCCCCGATCTCGTCGGCA
TTCTTCCAGACCGTCTCGCA

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MTDLCDMSAVDLRRLIGRGDVSPVELMDSCLDRIARINPAVNAVVALDTERARAAARRAEAAVRVGEPLGPLHGIPVGIK
DTEDTAGLRTTYGSPIHADNVPARDLGVVARLREAGAIVLAKTNTPEFAAGANTRNTVYGATGNAFDPTRSAAGSSGGSA
VALACGMVPLASGSDTGGSLRNPAAYAGIVGMRPSSGLVPNERRGLGWANLPVNGPMARTVGDAALMLGAMANDDPRDPL
AYTLPGEPVRGRADRYDPHRALDLGALRVAFTEDFGFAPTEQHVRRVFRHRVASLSPLFATAETATPDCAGADEAFAILR
AAQFLALHLDNYRTRPHLLGPNVRTNVEEGLGYTLADYARAASVQTAIYRTFVDFFRRYDLLISPTITLSPRPWTELYPT
EIDGAATRSYFHWLALAYGVTLAGHPAISLPLGVDEAGLPFGVQVVARRGGDADLLAAAAAIEAHCAGRPELTRPRPDLA
GLAGRAPIARSPGFFDLG

Sequences:

>Translated_498_residues
MTDLCDMSAVDLRRLIGRGDVSPVELMDSCLDRIARINPAVNAVVALDTERARAAARRAEAAVRVGEPLGPLHGIPVGIK
DTEDTAGLRTTYGSPIHADNVPARDLGVVARLREAGAIVLAKTNTPEFAAGANTRNTVYGATGNAFDPTRSAAGSSGGSA
VALACGMVPLASGSDTGGSLRNPAAYAGIVGMRPSSGLVPNERRGLGWANLPVNGPMARTVGDAALMLGAMANDDPRDPL
AYTLPGEPVRGRADRYDPHRALDLGALRVAFTEDFGFAPTEQHVRRVFRHRVASLSPLFATAETATPDCAGADEAFAILR
AAQFLALHLDNYRTRPHLLGPNVRTNVEEGLGYTLADYARAASVQTAIYRTFVDFFRRYDLLISPTITLSPRPWTELYPT
EIDGAATRSYFHWLALAYGVTLAGHPAISLPLGVDEAGLPFGVQVVARRGGDADLLAAAAAIEAHCAGRPELTRPRPDLA
GLAGRAPIARSPGFFDLG
>Mature_497_residues
TDLCDMSAVDLRRLIGRGDVSPVELMDSCLDRIARINPAVNAVVALDTERARAAARRAEAAVRVGEPLGPLHGIPVGIKD
TEDTAGLRTTYGSPIHADNVPARDLGVVARLREAGAIVLAKTNTPEFAAGANTRNTVYGATGNAFDPTRSAAGSSGGSAV
ALACGMVPLASGSDTGGSLRNPAAYAGIVGMRPSSGLVPNERRGLGWANLPVNGPMARTVGDAALMLGAMANDDPRDPLA
YTLPGEPVRGRADRYDPHRALDLGALRVAFTEDFGFAPTEQHVRRVFRHRVASLSPLFATAETATPDCAGADEAFAILRA
AQFLALHLDNYRTRPHLLGPNVRTNVEEGLGYTLADYARAASVQTAIYRTFVDFFRRYDLLISPTITLSPRPWTELYPTE
IDGAATRSYFHWLALAYGVTLAGHPAISLPLGVDEAGLPFGVQVVARRGGDADLLAAAAAIEAHCAGRPELTRPRPDLAG
LAGRAPIARSPGFFDLG

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=27.9207920792079, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI195972892, Length=506, Percent_Identity=26.6798418972332, Blast_Score=132, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17537465, Length=500, Percent_Identity=27.6, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17543272, Length=453, Percent_Identity=27.8145695364238, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71990152, Length=322, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=289, Percent_Identity=27.3356401384083, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=185, Percent_Identity=31.3513513513514, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17538254, Length=292, Percent_Identity=27.3972602739726, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=160, Percent_Identity=33.75, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=453, Percent_Identity=26.2693156732892, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320448, Length=230, Percent_Identity=29.5652173913043, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=438, Percent_Identity=24.8858447488584, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24648113, Length=494, Percent_Identity=30.3643724696356, Blast_Score=141, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24648435, Length=508, Percent_Identity=26.7716535433071, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24648437, Length=508, Percent_Identity=26.7716535433071, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI45550774, Length=509, Percent_Identity=26.9155206286837, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24652985, Length=524, Percent_Identity=25.5725190839695, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19922090, Length=524, Percent_Identity=25.5725190839695, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24652981, Length=524, Percent_Identity=25.5725190839695, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24652983, Length=524, Percent_Identity=25.5725190839695, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21356731, Length=498, Percent_Identity=27.1084337349398, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24644968, Length=250, Percent_Identity=31.6, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648441, Length=441, Percent_Identity=26.7573696145125, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648439, Length=441, Percent_Identity=26.7573696145125, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078093, Length=500, Percent_Identity=26.6, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 52510; Mature: 52379

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLCDMSAVDLRRLIGRGDVSPVELMDSCLDRIARINPAVNAVVALDTERARAAARRAE
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHEEEEEECCHHHHHHHHHHH
AAVRVGEPLGPLHGIPVGIKDTEDTAGLRTTYGSPIHADNVPARDLGVVARLREAGAIVL
HHHHCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEE
AKTNTPEFAAGANTRNTVYGATGNAFDPTRSAAGSSGGSAVALACGMVPLASGSDTGGSL
EECCCCCCCCCCCCCCEEEECCCCCCCCCHHHCCCCCCCEEEEEECCEEECCCCCCCCCC
RNPAAYAGIVGMRPSSGLVPNERRGLGWANLPVNGPMARTVGDAALMLGAMANDDPRDPL
CCCHHHHCEEECCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCE
AYTLPGEPVRGRADRYDPHRALDLGALRVAFTEDFGFAPTEQHVRRVFRHRVASLSPLFA
EEECCCCCCCCCCCCCCCHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHE
TAETATPDCAGADEAFAILRAAQFLALHLDNYRTRPHLLGPNVRTNVEEGLGYTLADYAR
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHCCCCHHHHHHH
AASVQTAIYRTFVDFFRRYDLLISPTITLSPRPWTELYPTEIDGAATRSYFHWLALAYGV
HHHHHHHHHHHHHHHHHHCCEEECCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TLAGHPAISLPLGVDEAGLPFGVQVVARRGGDADLLAAAAAIEAHCAGRPELTRPRPDLA
HCCCCCCEEECCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHH
GLAGRAPIARSPGFFDLG
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDLCDMSAVDLRRLIGRGDVSPVELMDSCLDRIARINPAVNAVVALDTERARAAARRAE
CCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHEEEEEECCHHHHHHHHHHH
AAVRVGEPLGPLHGIPVGIKDTEDTAGLRTTYGSPIHADNVPARDLGVVARLREAGAIVL
HHHHCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEE
AKTNTPEFAAGANTRNTVYGATGNAFDPTRSAAGSSGGSAVALACGMVPLASGSDTGGSL
EECCCCCCCCCCCCCCEEEECCCCCCCCCHHHCCCCCCCEEEEEECCEEECCCCCCCCCC
RNPAAYAGIVGMRPSSGLVPNERRGLGWANLPVNGPMARTVGDAALMLGAMANDDPRDPL
CCCHHHHCEEECCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCE
AYTLPGEPVRGRADRYDPHRALDLGALRVAFTEDFGFAPTEQHVRRVFRHRVASLSPLFA
EEECCCCCCCCCCCCCCCHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHE
TAETATPDCAGADEAFAILRAAQFLALHLDNYRTRPHLLGPNVRTNVEEGLGYTLADYAR
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHCCCCHHHHHHH
AASVQTAIYRTFVDFFRRYDLLISPTITLSPRPWTELYPTEIDGAATRSYFHWLALAYGV
HHHHHHHHHHHHHHHHHHCCEEECCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TLAGHPAISLPLGVDEAGLPFGVQVVARRGGDADLLAAAAAIEAHCAGRPELTRPRPDLA
HCCCCCCEEECCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHH
GLAGRAPIARSPGFFDLG
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]