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The map label for this gene is ptsN [H]

Identifier: 170746652

GI number: 170746652

Start: 211125

End: 211571

Strand: Reverse

Name: ptsN [H]

Synonym: Mrad2831_0203

Alternate gene names: 170746652

Gene position: 211571-211125 (Counterclockwise)

Preceding gene: 170746653

Following gene: 170746648

Centisome position: 3.48

GC content: 76.29

Gene sequence:

>447_bases
ATGACGATAGATCGGATCCTCGCGCCCGCCGACGTACTCGTCGGCCTGCGCGCCGCCAGCAAGAAGGCGCTGCTCGAGGA
GATGGCGTGCCGCGCGGCCAGCGCCCTCGGCCTCGCCGTGGAGACGGTCCTCTCGGCGCTGATCCGGCGCGAGACCCTGG
GCTCGACCGGGATCGGCGACGGCATCGCCCTGCCGCATGCGCGGCTCGAGGCTGTCGCGCGCCCGTACGGGATCCTGGCC
CGGCTCCGCGACCCGATCGAGTTCGATGCGGTCGACGACCGGCCGGTGGACCTCGTCGCTCTGCTCCTGCTCCCGACCGC
CGCCGAGGGCGAGACGCTGAACGCGCTGGCCTGCGTGGCGCGGCGCCTGCGCGACCCGGGGGCCGCTGCGGCGATGCGCG
GCGCGCGGGACGCGGCGGCGCTCCACGCGGCCGCGACGGGCCGTTAG

Upstream 100 bases:

>100_bases
GGACGACGAGCCGGCCCCGCATTCCCGCGGCGCCGACGGCGCGACCGCCCCCTGATTCCGAGGACGAGTTGATGCTTCGC
AGCGCCCGCGATCCCGCGCG

Downstream 100 bases:

>100_bases
GGGCGCCGCGCGGGCGTCAATGCGCGCCGCCGGTCTCCACGAACTTGAACAGGAAGACCGCGGCGATCACCCAGACGATC
GGCTTCACCTCCCGGGCGCG

Product: putative PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Nitrogen-metabolic PTS system EIIA component [H]

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MTIDRILAPADVLVGLRAASKKALLEEMACRAASALGLAVETVLSALIRRETLGSTGIGDGIALPHARLEAVARPYGILA
RLRDPIEFDAVDDRPVDLVALLLLPTAAEGETLNALACVARRLRDPGAAAAMRGARDAAALHAAATGR

Sequences:

>Translated_148_residues
MTIDRILAPADVLVGLRAASKKALLEEMACRAASALGLAVETVLSALIRRETLGSTGIGDGIALPHARLEAVARPYGILA
RLRDPIEFDAVDDRPVDLVALLLLPTAAEGETLNALACVARRLRDPGAAAAMRGARDAAALHAAATGR
>Mature_147_residues
TIDRILAPADVLVGLRAASKKALLEEMACRAASALGLAVETVLSALIRRETLGSTGIGDGIALPHARLEAVARPYGILAR
LRDPIEFDAVDDRPVDLVALLLLPTAAEGETLNALACVARRLRDPGAAAAMRGARDAAALHAAATGR

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=132, Percent_Identity=40.1515151515151, Blast_Score=96, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 15323; Mature: 15192

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDRILAPADVLVGLRAASKKALLEEMACRAASALGLAVETVLSALIRRETLGSTGIGD
CCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GIALPHARLEAVARPYGILARLRDPIEFDAVDDRPVDLVALLLLPTAAEGETLNALACVA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
RRLRDPGAAAAMRGARDAAALHAAATGR
HHHCCCCHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TIDRILAPADVLVGLRAASKKALLEEMACRAASALGLAVETVLSALIRRETLGSTGIGD
CHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GIALPHARLEAVARPYGILARLRDPIEFDAVDDRPVDLVALLLLPTAAEGETLNALACVA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
RRLRDPGAAAAMRGARDAAALHAAATGR
HHHCCCCHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 1991712 [H]