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The map label for this gene is mcbR [H]

Identifier: 170746615

GI number: 170746615

Start: 162742

End: 163410

Strand: Reverse

Name: mcbR [H]

Synonym: Mrad2831_0165

Alternate gene names: 170746615

Gene position: 163410-162742 (Counterclockwise)

Preceding gene: 170746624

Following gene: 170746613

Centisome position: 2.69

GC content: 73.24

Gene sequence:

>669_bases
ATGCACGGAGACTTCCGGAAGATCGACAGCGGGCTGCTCTCGGACCGGATCCGCGACGCCCTCACCGACGCCATCGCGTC
GGGGACGATCGCGGCCGGCACGGCCCTCGACGAGCAGAACCTGGCCGATCGCTACGGCGCGTCGCGCACGCCCGTCCGGG
AGGCGTTACGCCAGCTCGCCGCCTCCGGGCTCGTGGAGATCCGGCCCCGCCGGGGCGTGGTGGTGGCGCGCCTGACGCCG
CAGCGGATCGCCGACATGTTCGAGACGACCGCCGAGATCGAGGCGATGTGCGCTCGACTCGCGACCTACCGGATGACGCC
CCTCGAGCGCGGGGAGCTGATGGAGCTCCACGAGGAGTCCGCCCAGGCGGTCGAGGCGGGCGACGTCGACGCCTACGACC
GATTCAACCGTGCCTTCCACGAGGCGCTCTACACGGCGACCCATAACGGCTTCATGGCCGAGCAGGCCCTGGCGATCCGC
GAGCGGCTGGCCGCGTTCCGGCGCACGCAGCTCCGCCATGCCGACCGGATCCGCCGCTCGCGGGAGGAGCACGGCGAGAT
CCTGGCGGCGATCGCCGAGGGCGACGGCGAGACGGCCGCCCGGCGCATGCGCGCCCACATGCTCCGGGCCGCGGCGGCGC
TGGGGCGCTACATCGCCGAGACGGAGTGA

Upstream 100 bases:

>100_bases
GGTCGAATTGCGGGCGTGCGCGCGCGGATTAGGGTATTGTTCCTCATCGTCTTCCCGAGGACTGGCAGGCGTCGCTGAAG
GGACGATCGAGGAACGGCGG

Downstream 100 bases:

>100_bases
CGATGCGGACAGTCGCGCACGGGATTCAGAGGCGCGCGACGGCGTCGTGCACCGTGGACCTGTAGGGCGTCGTCGGCCTG
CCGATCAGCGCGCTCAGCTG

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MHGDFRKIDSGLLSDRIRDALTDAIASGTIAAGTALDEQNLADRYGASRTPVREALRQLAASGLVEIRPRRGVVVARLTP
QRIADMFETTAEIEAMCARLATYRMTPLERGELMELHEESAQAVEAGDVDAYDRFNRAFHEALYTATHNGFMAEQALAIR
ERLAAFRRTQLRHADRIRRSREEHGEILAAIAEGDGETAARRMRAHMLRAAAALGRYIAETE

Sequences:

>Translated_222_residues
MHGDFRKIDSGLLSDRIRDALTDAIASGTIAAGTALDEQNLADRYGASRTPVREALRQLAASGLVEIRPRRGVVVARLTP
QRIADMFETTAEIEAMCARLATYRMTPLERGELMELHEESAQAVEAGDVDAYDRFNRAFHEALYTATHNGFMAEQALAIR
ERLAAFRRTQLRHADRIRRSREEHGEILAAIAEGDGETAARRMRAHMLRAAAALGRYIAETE
>Mature_222_residues
MHGDFRKIDSGLLSDRIRDALTDAIASGTIAAGTALDEQNLADRYGASRTPVREALRQLAASGLVEIRPRRGVVVARLTP
QRIADMFETTAEIEAMCARLATYRMTPLERGELMELHEESAQAVEAGDVDAYDRFNRAFHEALYTATHNGFMAEQALAIR
ERLAAFRRTQLRHADRIRRSREEHGEILAAIAEGDGETAARRMRAHMLRAAAALGRYIAETE

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: COG1802

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=187, Percent_Identity=27.807486631016, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1787821, Length=184, Percent_Identity=27.1739130434783, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI87082149, Length=197, Percent_Identity=29.9492385786802, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24689; Mature: 24689

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGDFRKIDSGLLSDRIRDALTDAIASGTIAAGTALDEQNLADRYGASRTPVREALRQLA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHH
ASGLVEIRPRRGVVVARLTPQRIADMFETTAEIEAMCARLATYRMTPLERGELMELHEES
HCCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AQAVEAGDVDAYDRFNRAFHEALYTATHNGFMAEQALAIRERLAAFRRTQLRHADRIRRS
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REEHGEILAAIAEGDGETAARRMRAHMLRAAAALGRYIAETE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHGDFRKIDSGLLSDRIRDALTDAIASGTIAAGTALDEQNLADRYGASRTPVREALRQLA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHH
ASGLVEIRPRRGVVVARLTPQRIADMFETTAEIEAMCARLATYRMTPLERGELMELHEES
HCCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AQAVEAGDVDAYDRFNRAFHEALYTATHNGFMAEQALAIRERLAAFRRTQLRHADRIRRS
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REEHGEILAAIAEGDGETAARRMRAHMLRAAAALGRYIAETE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]