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The map label for this gene is proB [H]

Identifier: 170746554

GI number: 170746554

Start: 109859

End: 110974

Strand: Reverse

Name: proB [H]

Synonym: Mrad2831_0104

Alternate gene names: 170746554

Gene position: 110974-109859 (Counterclockwise)

Preceding gene: 170746555

Following gene: 170746553

Centisome position: 1.83

GC content: 71.86

Gene sequence:

>1116_bases
ATGATCCCTGCCCTCGAAGAATTTCGCCGTGTGGTCATCAAGGTCGGCTCGGCCCTGCTGGTCGACCGCAGTGCCGGCCG
CCTGCGCCATGCCTGGCTCGCGGCGCTGGCCGAGGACATCGCGGAGTTGCACGGGCGCGGCGTCGACGTTCTGGTCGTGT
CGTCCGGCAGCATCGCCCTCGGCCGGACGGTGCTGGGTTTGCCGCCCGGCGTCCTGCGCCTGGAGGAGAGCCAGGGCGCG
GCCTCGGTGGGGCAGATCACGCTGGCGCGCCACTGGGCCGAAGCTCTGGGCCATCACGGCATCGTCGCAGGCCAGATCCT
CGTGACGCCGCAGGACACCGAGGAGCGCCGCCGCTACCTCAACGCCCGCGCCACCGTGCTGAAGCTCCTGGAGATGCGCG
CGGTCCCGGTCGTGAACGAGAACGACACGGTGGCGACCTCGGAGATCCGCTACGGCGACAACGACCGGCTCGCCGCCCGG
GTCGCCACCATGATCGACGCCGACGTGCTGGTGCTGTTCTCGGACATCGACGGCCTCTACACCGCGCCGCCCAAGAGCGA
TCCGGACGCCCGCCACCTCGCCGTGGTCGAGCGCATCACCCCGGAGATCGAGGCGATGGCCGGCGGCCCGGCCTCCGAGC
TGTCGCGGGGCGGCATGCGCACCAAGGTCGAGGCCGCCAAGATCGCCGCCTCGGGCGGCACGCACCTGGTCATCGCGGAC
GGACGCGGGAAGAACCCGCTCAAGGCCGTGCGGGACGGCGCGCGCTGCACGTGGTTCCTGTCGGGCTCCACGCCGACGGC
CGCCCGGAAGACCTGGATCGCCGGCTCCCTCGAGCCACGCGGCACGCTGACGATCGACGCGGGTGCCGAGCAGGCGCTCC
GGAGCGGGGCCAGCCTGCTCCCGGTGGGCGTGCGGGCGATCGAGGGCAGCTTCTCGCGCGGCGACGCCGTGATGATCCGC
GATCCGGAGGGCCGGATCCTGGGCCGCGGCCTGGTGGCGTACGACAGCGCCGAGGCCGCGCTGATCATCGGTCATCCGAG
CGCGCGCATTCCGGAACTGCTCAACTATCCCGGTCGCGCCTGGATGGTGCACCGCGATGACTTGGCATTGTTCTAG

Upstream 100 bases:

>100_bases
CGGAAGCCGGTAGCCGCCTGGCAGCCGTAGCCCTCGACCGACTGTTCAAAGGGCGGCTCTTGGTTCACACCCGCCGTCGA
CGCGCCGTCCCGCAAGAATC

Downstream 100 bases:

>100_bases
AAGTCGCGGACATAAGCTAGTCTATATACAATTTTTCCTCTCAATTACCGTCACAATTCCCGGCCCGGCACTCTTGTCGG
GACGGCGCTCGATGTGCAAA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MIPALEEFRRVVIKVGSALLVDRSAGRLRHAWLAALAEDIAELHGRGVDVLVVSSGSIALGRTVLGLPPGVLRLEESQGA
ASVGQITLARHWAEALGHHGIVAGQILVTPQDTEERRRYLNARATVLKLLEMRAVPVVNENDTVATSEIRYGDNDRLAAR
VATMIDADVLVLFSDIDGLYTAPPKSDPDARHLAVVERITPEIEAMAGGPASELSRGGMRTKVEAAKIAASGGTHLVIAD
GRGKNPLKAVRDGARCTWFLSGSTPTAARKTWIAGSLEPRGTLTIDAGAEQALRSGASLLPVGVRAIEGSFSRGDAVMIR
DPEGRILGRGLVAYDSAEAALIIGHPSARIPELLNYPGRAWMVHRDDLALF

Sequences:

>Translated_371_residues
MIPALEEFRRVVIKVGSALLVDRSAGRLRHAWLAALAEDIAELHGRGVDVLVVSSGSIALGRTVLGLPPGVLRLEESQGA
ASVGQITLARHWAEALGHHGIVAGQILVTPQDTEERRRYLNARATVLKLLEMRAVPVVNENDTVATSEIRYGDNDRLAAR
VATMIDADVLVLFSDIDGLYTAPPKSDPDARHLAVVERITPEIEAMAGGPASELSRGGMRTKVEAAKIAASGGTHLVIAD
GRGKNPLKAVRDGARCTWFLSGSTPTAARKTWIAGSLEPRGTLTIDAGAEQALRSGASLLPVGVRAIEGSFSRGDAVMIR
DPEGRILGRGLVAYDSAEAALIIGHPSARIPELLNYPGRAWMVHRDDLALF
>Mature_371_residues
MIPALEEFRRVVIKVGSALLVDRSAGRLRHAWLAALAEDIAELHGRGVDVLVVSSGSIALGRTVLGLPPGVLRLEESQGA
ASVGQITLARHWAEALGHHGIVAGQILVTPQDTEERRRYLNARATVLKLLEMRAVPVVNENDTVATSEIRYGDNDRLAAR
VATMIDADVLVLFSDIDGLYTAPPKSDPDARHLAVVERITPEIEAMAGGPASELSRGGMRTKVEAAKIAASGGTHLVIAD
GRGKNPLKAVRDGARCTWFLSGSTPTAARKTWIAGSLEPRGTLTIDAGAEQALRSGASLLPVGVRAIEGSFSRGDAVMIR
DPEGRILGRGLVAYDSAEAALIIGHPSARIPELLNYPGRAWMVHRDDLALF

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=268, Percent_Identity=32.089552238806, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI62912457, Length=266, Percent_Identity=32.3308270676692, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=40.4371584699454, Blast_Score=242, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17569969, Length=260, Percent_Identity=30.7692307692308, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17569967, Length=260, Percent_Identity=30.7692307692308, Blast_Score=121, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6320506, Length=414, Percent_Identity=31.6425120772947, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321822, Length=413, Percent_Identity=32.4455205811138, Blast_Score=182, Evalue=7e-47,
Organism=Drosophila melanogaster, GI21357643, Length=294, Percent_Identity=29.5918367346939, Blast_Score=118, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39454; Mature: 39454

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPALEEFRRVVIKVGSALLVDRSAGRLRHAWLAALAEDIAELHGRGVDVLVVSSGSIAL
CCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEE
GRTVLGLPPGVLRLEESQGAASVGQITLARHWAEALGHHGIVAGQILVTPQDTEERRRYL
CCEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHH
NARATVLKLLEMRAVPVVNENDTVATSEIRYGDNDRLAARVATMIDADVLVLFSDIDGLY
HHHHHHHHHHHHHCCCEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCE
TAPPKSDPDARHLAVVERITPEIEAMAGGPASELSRGGMRTKVEAAKIAASGGTHLVIAD
ECCCCCCCCHHHHHHHHHHCCHHHHHCCCCHHHHHCCCCCEEEHHHEEEECCCCEEEEEC
GRGKNPLKAVRDGARCTWFLSGSTPTAARKTWIAGSLEPRGTLTIDAGAEQALRSGASLL
CCCCCHHHHHCCCCEEEEEECCCCCCHHHHEEEECCCCCCCEEEEECCHHHHHHCCCCEE
PVGVRAIEGSFSRGDAVMIRDPEGRILGRGLVAYDSAEAALIIGHPSARIPELLNYPGRA
EECCEEECCCCCCCCEEEEECCCCCEEECEEEEECCCCEEEEEECCCCCCHHHHCCCCCE
WMVHRDDLALF
EEEECCCCCCC
>Mature Secondary Structure
MIPALEEFRRVVIKVGSALLVDRSAGRLRHAWLAALAEDIAELHGRGVDVLVVSSGSIAL
CCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEE
GRTVLGLPPGVLRLEESQGAASVGQITLARHWAEALGHHGIVAGQILVTPQDTEERRRYL
CCEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHH
NARATVLKLLEMRAVPVVNENDTVATSEIRYGDNDRLAARVATMIDADVLVLFSDIDGLY
HHHHHHHHHHHHHCCCEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCE
TAPPKSDPDARHLAVVERITPEIEAMAGGPASELSRGGMRTKVEAAKIAASGGTHLVIAD
ECCCCCCCCHHHHHHHHHHCCHHHHHCCCCHHHHHCCCCCEEEHHHEEEECCCCEEEEEC
GRGKNPLKAVRDGARCTWFLSGSTPTAARKTWIAGSLEPRGTLTIDAGAEQALRSGASLL
CCCCCHHHHHCCCCEEEEEECCCCCCHHHHEEEECCCCCCCEEEEECCHHHHHHCCCCEE
PVGVRAIEGSFSRGDAVMIRDPEGRILGRGLVAYDSAEAALIIGHPSARIPELLNYPGRA
EECCEEECCCCCCCCEEEEECCCCCEEECEEEEECCCCEEEEEECCCCCCHHHHCCCCCE
WMVHRDDLALF
EEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA