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The map label for this gene is alkK [H]

Identifier: 170746513

GI number: 170746513

Start: 66877

End: 68529

Strand: Reverse

Name: alkK [H]

Synonym: Mrad2831_0063

Alternate gene names: 170746513

Gene position: 68529-66877 (Counterclockwise)

Preceding gene: 170746518

Following gene: 170746504

Centisome position: 1.13

GC content: 71.87

Gene sequence:

>1653_bases
ATGACGGGAACCCGTCCGACCCGAGCCGAACTCCGTGAGAACGGCTCCCACCCGTTCGAGGCCGGGCTCGCGCGCCGGGC
GGCCAACTACGTGCCCCTGACCCCCGTGAGCCTGCTGGCGCGGGCCGCCGCCGCGGCGGGCGCGCGGACCGCCGTGATCG
ACGGCGACCGGCGCCTCACCTACGCGGACCTCTACGCGCGCTGCCGGCGCCTGGCCGCGGGCCTCGCGGCCCGGGGGATC
GGGCCGCTCGACACCGTGGCGATCCTTGCGCCGAACGTCCCGGAGATGATCGAGGCGCATTTCGCCGTGCCGATGCTCGG
CGCGGTGCTGAACCCGCTCAACACCCGCCTCGACGCGGCGACGATCGCGTTCTCGCTGCGGCACGGGGGCGCCCGGGCGC
TCCTCGTCGAGGCCGAGTATGCCGGTCTGGCCGCGCGCGCCCTGGCGGATCTCGCCGAGCCGATCCTCGTCGTGGCGATC
GGCGATTCCGGGATCGACGGGGCGCTGTCCTACGGGGATCTGCTGGCGGAGGGCGATCCGGACTACGCCTGGGCCGGCCC
CGAGGACGAGTGGCAGTCGCTCTGCCTGCTCTACACGTCGGGGACGACCGGCGACCCGAAAGGCGCCGTCTACAGCCATC
GCGGCGGCTATCTCCAGGCCCTGGGCAACGCCGTGACCTTCGGCCTGACCGGCGAGAGCGTGTATCTCTGGACGCTGCCG
ATGTTCCACTGCAGCGGCTGGTCCTACCCCTGGGCGAGCGTCGCGGCCTGCGCCACGCAGGTTTGCCTCCGCAAGGTTGA
GCCGGCGGCGATCTTCCGGCTGATCGCCGAGCACGGGGTGACGCATCTGTGCGGGGCGCCGATCGTCCTCTCGATGATCG
CGCACGCCCCGGCCCAGGACCGGGTGCCGTTCCCACAGCGGGTGCGCTGCGCGGTCGGCGGCGCGGCGCCGTCCTCCACG
ATCATCCGCACCATGGAGGAGCTGGGCTTCCAGGTGACTCATCTCTACGGCGCCACCGAGAGTTACGGTCCCGCGACGGT
CTGCCTGGCGCAGCCGGACTGGACCGACCTCCCCGATACCGAGCGCTACGCCCGCATGGCCCGGCAGGGCGTGCCCCTGG
CGACCCTCGAGGCGGTGAGCGTGGCCGACACCGCGACCGACGTGCCGGTGCCGCGGGACGGCACGACGGTCGGCGAGATC
CGTCTGCGCGGCAACACGGTGATGAAGGGCTACCTCGGCAACCCGGGGGCGACCGAGGCGGCCCTCGCCGACGGCTGGTA
CCGGACCGGCGACCTCGCGGTGTGGCATCCCGACGGGTCGGTGGAGATCAAGGACCGGGCGAAGGACATCATCATCTCGG
GCGGCGAGAATATCTCCAGCCTGGAGGTGGAGGAGGTGCTCATGCGCCACCCCGCAGTGATGCTGGCGGCCGTCGTGGCG
CGGCCGGATCCGACCTGGGGCGAGAGCCCCTGCGCGTTCCTAGAGGTGAAGCCCGGCACGAGCGTGCCGAGCGAGCAGGA
GTTGATCGCGTTCTGTCGCGAGCACATGGCGCGGTTCAAGGTGCCGAAGACGGTCGTGTTCGGGCCTTTGCCGAAGACCT
CGACCGGCAAGATCCAGAAATTCGTCCTGCGCGAGCAGGCGCGGGATCTCTGA

Upstream 100 bases:

>100_bases
CACATTGTCCCGGCGACCGCGATTGACAGGCGGGGCGGCCGCGAAATCATCGTCGCGCGGAGCGCACGCCACGCCCGCCG
CCCGATCAGAAGGCTGCACG

Downstream 100 bases:

>100_bases
CCCGCTTCGGACCCGAGGACGAGCTCGTCCGGGGCGATCGCTACAGGCCGGAGACGAGGTGGCCGCCGTCGACCGCCAGG
ACGCTGCCGGTCATGTAGGC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MTGTRPTRAELRENGSHPFEAGLARRAANYVPLTPVSLLARAAAAAGARTAVIDGDRRLTYADLYARCRRLAAGLAARGI
GPLDTVAILAPNVPEMIEAHFAVPMLGAVLNPLNTRLDAATIAFSLRHGGARALLVEAEYAGLAARALADLAEPILVVAI
GDSGIDGALSYGDLLAEGDPDYAWAGPEDEWQSLCLLYTSGTTGDPKGAVYSHRGGYLQALGNAVTFGLTGESVYLWTLP
MFHCSGWSYPWASVAACATQVCLRKVEPAAIFRLIAEHGVTHLCGAPIVLSMIAHAPAQDRVPFPQRVRCAVGGAAPSST
IIRTMEELGFQVTHLYGATESYGPATVCLAQPDWTDLPDTERYARMARQGVPLATLEAVSVADTATDVPVPRDGTTVGEI
RLRGNTVMKGYLGNPGATEAALADGWYRTGDLAVWHPDGSVEIKDRAKDIIISGGENISSLEVEEVLMRHPAVMLAAVVA
RPDPTWGESPCAFLEVKPGTSVPSEQELIAFCREHMARFKVPKTVVFGPLPKTSTGKIQKFVLREQARDL

Sequences:

>Translated_550_residues
MTGTRPTRAELRENGSHPFEAGLARRAANYVPLTPVSLLARAAAAAGARTAVIDGDRRLTYADLYARCRRLAAGLAARGI
GPLDTVAILAPNVPEMIEAHFAVPMLGAVLNPLNTRLDAATIAFSLRHGGARALLVEAEYAGLAARALADLAEPILVVAI
GDSGIDGALSYGDLLAEGDPDYAWAGPEDEWQSLCLLYTSGTTGDPKGAVYSHRGGYLQALGNAVTFGLTGESVYLWTLP
MFHCSGWSYPWASVAACATQVCLRKVEPAAIFRLIAEHGVTHLCGAPIVLSMIAHAPAQDRVPFPQRVRCAVGGAAPSST
IIRTMEELGFQVTHLYGATESYGPATVCLAQPDWTDLPDTERYARMARQGVPLATLEAVSVADTATDVPVPRDGTTVGEI
RLRGNTVMKGYLGNPGATEAALADGWYRTGDLAVWHPDGSVEIKDRAKDIIISGGENISSLEVEEVLMRHPAVMLAAVVA
RPDPTWGESPCAFLEVKPGTSVPSEQELIAFCREHMARFKVPKTVVFGPLPKTSTGKIQKFVLREQARDL
>Mature_549_residues
TGTRPTRAELRENGSHPFEAGLARRAANYVPLTPVSLLARAAAAAGARTAVIDGDRRLTYADLYARCRRLAAGLAARGIG
PLDTVAILAPNVPEMIEAHFAVPMLGAVLNPLNTRLDAATIAFSLRHGGARALLVEAEYAGLAARALADLAEPILVVAIG
DSGIDGALSYGDLLAEGDPDYAWAGPEDEWQSLCLLYTSGTTGDPKGAVYSHRGGYLQALGNAVTFGLTGESVYLWTLPM
FHCSGWSYPWASVAACATQVCLRKVEPAAIFRLIAEHGVTHLCGAPIVLSMIAHAPAQDRVPFPQRVRCAVGGAAPSSTI
IRTMEELGFQVTHLYGATESYGPATVCLAQPDWTDLPDTERYARMARQGVPLATLEAVSVADTATDVPVPRDGTTVGEIR
LRGNTVMKGYLGNPGATEAALADGWYRTGDLAVWHPDGSVEIKDRAKDIIISGGENISSLEVEEVLMRHPAVMLAAVVAR
PDPTWGESPCAFLEVKPGTSVPSEQELIAFCREHMARFKVPKTVVFGPLPKTSTGKIQKFVLREQARDL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=550, Percent_Identity=29.2727272727273, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI157311624, Length=477, Percent_Identity=25.5765199161426, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI157311622, Length=477, Percent_Identity=25.5765199161426, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI58082049, Length=478, Percent_Identity=25.9414225941423, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI38505220, Length=503, Percent_Identity=26.441351888668, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI187761345, Length=541, Percent_Identity=26.9870609981516, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI187761343, Length=541, Percent_Identity=26.9870609981516, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI42544132, Length=510, Percent_Identity=25.6862745098039, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI122937307, Length=504, Percent_Identity=26.5873015873016, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI115511026, Length=517, Percent_Identity=25.1450676982592, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI42794756, Length=484, Percent_Identity=27.8925619834711, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI42794760, Length=484, Percent_Identity=27.8925619834711, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI42794758, Length=484, Percent_Identity=27.8925619834711, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI62865631, Length=479, Percent_Identity=26.7223382045929, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI13325055, Length=479, Percent_Identity=26.7223382045929, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI40807491, Length=349, Percent_Identity=28.080229226361, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI38524616, Length=440, Percent_Identity=25.4545454545455, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI13236579, Length=419, Percent_Identity=27.2076372315036, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI227499619, Length=486, Percent_Identity=22.6337448559671, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI28416953, Length=124, Percent_Identity=35.4838709677419, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI145693145, Length=521, Percent_Identity=29.9424184261036, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1786810, Length=536, Percent_Identity=29.4776119402985, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788107, Length=547, Percent_Identity=25.7769652650823, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI221142682, Length=519, Percent_Identity=27.1676300578035, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1788595, Length=501, Percent_Identity=25.3493013972056, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786801, Length=480, Percent_Identity=25.4166666666667, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789201, Length=367, Percent_Identity=24.7956403269755, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71994694, Length=535, Percent_Identity=28.411214953271, Blast_Score=176, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI71994703, Length=535, Percent_Identity=28.411214953271, Blast_Score=176, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI71994690, Length=535, Percent_Identity=28.411214953271, Blast_Score=176, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17560308, Length=532, Percent_Identity=26.5037593984962, Blast_Score=155, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI32563687, Length=517, Percent_Identity=27.852998065764, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17559526, Length=368, Percent_Identity=29.6195652173913, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17558820, Length=514, Percent_Identity=27.431906614786, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17538037, Length=536, Percent_Identity=25.5597014925373, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=535, Percent_Identity=24.8598130841122, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=372, Percent_Identity=23.9247311827957, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=512, Percent_Identity=22.8515625, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115534440, Length=458, Percent_Identity=24.8908296943231, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17556552, Length=506, Percent_Identity=24.703557312253, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71983001, Length=535, Percent_Identity=24.6728971962617, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71982997, Length=535, Percent_Identity=24.6728971962617, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=362, Percent_Identity=26.2430939226519, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17510401, Length=192, Percent_Identity=28.125, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=540, Percent_Identity=24.8148148148148, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6323182, Length=556, Percent_Identity=25, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323903, Length=360, Percent_Identity=25.5555555555556, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24581924, Length=539, Percent_Identity=28.5714285714286, Blast_Score=145, Evalue=1e-34,
Organism=Drosophila melanogaster, GI18859661, Length=520, Percent_Identity=29.0384615384615, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21355181, Length=523, Percent_Identity=24.8565965583174, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI19922652, Length=506, Percent_Identity=24.1106719367589, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356947, Length=496, Percent_Identity=21.9758064516129, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45552801, Length=456, Percent_Identity=24.1228070175439, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45550496, Length=549, Percent_Identity=23.6794171220401, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281365686, Length=479, Percent_Identity=22.5469728601253, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24583465, Length=464, Percent_Identity=23.4913793103448, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583463, Length=464, Percent_Identity=23.4913793103448, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356441, Length=504, Percent_Identity=22.6190476190476, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364798, Length=464, Percent_Identity=23.4913793103448, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI62472339, Length=128, Percent_Identity=39.0625, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24667955, Length=128, Percent_Identity=39.0625, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24667959, Length=128, Percent_Identity=39.0625, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI20130357, Length=503, Percent_Identity=21.6699801192843, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24653035, Length=493, Percent_Identity=22.3123732251521, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 58553; Mature: 58422

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTRPTRAELRENGSHPFEAGLARRAANYVPLTPVSLLARAAAAAGARTAVIDGDRRLT
CCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCH
YADLYARCRRLAAGLAARGIGPLDTVAILAPNVPEMIEAHFAVPMLGAVLNPLNTRLDAA
HHHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIAFSLRHGGARALLVEAEYAGLAARALADLAEPILVVAIGDSGIDGALSYGDLLAEGDP
HEEEEEECCCCEEEEEECHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCCC
DYAWAGPEDEWQSLCLLYTSGTTGDPKGAVYSHRGGYLQALGNAVTFGLTGESVYLWTLP
CCCCCCCCHHHCCEEEEEECCCCCCCCCCEECCCCCHHHHCCCEEEEEECCCEEEEEEEE
MFHCSGWSYPWASVAACATQVCLRKVEPAAIFRLIAEHGVTHLCGAPIVLSMIAHAPAQD
HHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHCCHHHHHHHHHCCCCCC
RVPFPQRVRCAVGGAAPSSTIIRTMEELGFQVTHLYGATESYGPATVCLAQPDWTDLPDT
CCCCCHHHHEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCH
ERYARMARQGVPLATLEAVSVADTATDVPVPRDGTTVGEIRLRGNTVMKGYLGNPGATEA
HHHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCHH
ALADGWYRTGDLAVWHPDGSVEIKDRAKDIIISGGENISSLEVEEVLMRHPAVMLAAVVA
HHHCCCEECCCEEEECCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHH
RPDPTWGESPCAFLEVKPGTSVPSEQELIAFCREHMARFKVPKTVVFGPLPKTSTGKIQK
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHH
FVLREQARDL
HHHHHHHHCC
>Mature Secondary Structure 
TGTRPTRAELRENGSHPFEAGLARRAANYVPLTPVSLLARAAAAAGARTAVIDGDRRLT
CCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCH
YADLYARCRRLAAGLAARGIGPLDTVAILAPNVPEMIEAHFAVPMLGAVLNPLNTRLDAA
HHHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIAFSLRHGGARALLVEAEYAGLAARALADLAEPILVVAIGDSGIDGALSYGDLLAEGDP
HEEEEEECCCCEEEEEECHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCCC
DYAWAGPEDEWQSLCLLYTSGTTGDPKGAVYSHRGGYLQALGNAVTFGLTGESVYLWTLP
CCCCCCCCHHHCCEEEEEECCCCCCCCCCEECCCCCHHHHCCCEEEEEECCCEEEEEEEE
MFHCSGWSYPWASVAACATQVCLRKVEPAAIFRLIAEHGVTHLCGAPIVLSMIAHAPAQD
HHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHCCHHHHHHHHHCCCCCC
RVPFPQRVRCAVGGAAPSSTIIRTMEELGFQVTHLYGATESYGPATVCLAQPDWTDLPDT
CCCCCHHHHEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCH
ERYARMARQGVPLATLEAVSVADTATDVPVPRDGTTVGEIRLRGNTVMKGYLGNPGATEA
HHHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCHH
ALADGWYRTGDLAVWHPDGSVEIKDRAKDIIISGGENISSLEVEEVLMRHPAVMLAAVVA
HHHCCCEECCCEEEECCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHH
RPDPTWGESPCAFLEVKPGTSVPSEQELIAFCREHMARFKVPKTVVFGPLPKTSTGKIQK
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHH
FVLREQARDL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]