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The map label for this gene is ycgF [H]

Identifier: 170746455

GI number: 170746455

Start: 4411

End: 5190

Strand: Reverse

Name: ycgF [H]

Synonym: Mrad2831_0005

Alternate gene names: 170746455

Gene position: 5190-4411 (Counterclockwise)

Preceding gene: 170746457

Following gene: NA

Centisome position: 0.09

GC content: 69.23

Gene sequence:

>780_bases
ATGACGAAGGATTCCGGCTGCCGCGCCTGTCGCGCCGGCACCAAGCTCGACTTCGACTTCACGATGGCGTTTCAGCCGAT
CCTCGACCTCGCGGACGGCAGCGTCTGGGCCTACGAGGCGCTGGTGCGCGGCCCGAACGGCGAGCCAGCCTACACGATCC
TCGACCGGGTGACCGACGACACCCGCTACCAGTTCGATCAGGCCGCCCGCGTCAAGGCGATCGAACTCGCCGGCACCCTG
TTCCCCCGCGACAGCGCCACGCGGCTGTCGATCAACTTCATGCCGAACGCGGTCTACGAGCCGAATGCCTGCATCCGCGC
CTCGCTCGAGGCGGCCCGGCGCATCGACTTCGCCCACCGACGCATCATGTTCGAGTTCACCGAGCAGGAGCGGTTTCGCG
ACCTCGAGCACGTGAAGGGCATCGTCGCCGCCTACCGGAAGCAGGGATTCCTGACGGCGCTCGACGATTTCGGCGCCGGC
TTCGCGGGCTTGAGCCTGCTCGCCAACTTCCGCCCCGACCTGATCAAGATCGACATGGACGTGCTGCGCGGCCTCGACAA
CGACGCCGGGCGCTACGCCATCGTCTCGGGCGTGGTCGCCATCGCCCGCGCCCTCGACGTCACGGTGCTGGCCGAGGGCG
TGGAGACCGCCGGGGAGCTCGACGCCCTGCGAAACATCGGGATCACGCTCGTGCAGGGCTACCACTTCGCCAAGCCCCTC
CTGGAGGGACTGCCCCCGGTCGCGGGCTTCGCCCCCGTGGCTGCCGCGCGCGCAGCCTGA

Upstream 100 bases:

>100_bases
CTGGATTCCGCCGACGATCCGCCGGCGCGCTTGCATTAAGGTTGTGTTGAGCGCGGCCGCCCATTGTCCGCCGGCCGGGG
TCACTCACCGAAAGCCGCAC

Downstream 100 bases:

>100_bases
CGCCGGACCGACGCGCGCGGCCTTCATCGCCTACTTCGGAGCCGGGGTCGGCGCCGCGGCCGGCGGCACGGGCGGCACCG
GCGGGGTGCCGGGCTTCGGC

Product: diguanylate phosphodiesterase

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTKDSGCRACRAGTKLDFDFTMAFQPILDLADGSVWAYEALVRGPNGEPAYTILDRVTDDTRYQFDQAARVKAIELAGTL
FPRDSATRLSINFMPNAVYEPNACIRASLEAARRIDFAHRRIMFEFTEQERFRDLEHVKGIVAAYRKQGFLTALDDFGAG
FAGLSLLANFRPDLIKIDMDVLRGLDNDAGRYAIVSGVVAIARALDVTVLAEGVETAGELDALRNIGITLVQGYHFAKPL
LEGLPPVAGFAPVAAARAA

Sequences:

>Translated_259_residues
MTKDSGCRACRAGTKLDFDFTMAFQPILDLADGSVWAYEALVRGPNGEPAYTILDRVTDDTRYQFDQAARVKAIELAGTL
FPRDSATRLSINFMPNAVYEPNACIRASLEAARRIDFAHRRIMFEFTEQERFRDLEHVKGIVAAYRKQGFLTALDDFGAG
FAGLSLLANFRPDLIKIDMDVLRGLDNDAGRYAIVSGVVAIARALDVTVLAEGVETAGELDALRNIGITLVQGYHFAKPL
LEGLPPVAGFAPVAAARAA
>Mature_258_residues
TKDSGCRACRAGTKLDFDFTMAFQPILDLADGSVWAYEALVRGPNGEPAYTILDRVTDDTRYQFDQAARVKAIELAGTLF
PRDSATRLSINFMPNAVYEPNACIRASLEAARRIDFAHRRIMFEFTEQERFRDLEHVKGIVAAYRKQGFLTALDDFGAGF
AGLSLLANFRPDLIKIDMDVLRGLDNDAGRYAIVSGVVAIARALDVTVLAEGVETAGELDALRNIGITLVQGYHFAKPLL
EGLPPVAGFAPVAAARAA

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=246, Percent_Identity=30.8943089430894, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI226510982, Length=238, Percent_Identity=26.890756302521, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1790496, Length=241, Percent_Identity=26.9709543568465, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87081845, Length=246, Percent_Identity=29.2682926829268, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI87081743, Length=233, Percent_Identity=27.0386266094421, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081980, Length=236, Percent_Identity=25, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787541, Length=131, Percent_Identity=35.1145038167939, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082096, Length=227, Percent_Identity=27.7533039647577, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788849, Length=154, Percent_Identity=29.2207792207792, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788502, Length=235, Percent_Identity=24.6808510638298, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081921, Length=120, Percent_Identity=32.5, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1786507, Length=120, Percent_Identity=30.8333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787055, Length=228, Percent_Identity=27.1929824561404, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 28263; Mature: 28131

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKDSGCRACRAGTKLDFDFTMAFQPILDLADGSVWAYEALVRGPNGEPAYTILDRVTDD
CCCCCCCHHHHCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHCCC
TRYQFDQAARVKAIELAGTLFPRDSATRLSINFMPNAVYEPNACIRASLEAARRIDFAHR
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
RIMFEFTEQERFRDLEHVKGIVAAYRKQGFLTALDDFGAGFAGLSLLANFRPDLIKIDMD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCHHHHHHHHHHCCCCCEEECHH
VLRGLDNDAGRYAIVSGVVAIARALDVTVLAEGVETAGELDALRNIGITLVQGYHFAKPL
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHH
LEGLPPVAGFAPVAAARAA
HHCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
TKDSGCRACRAGTKLDFDFTMAFQPILDLADGSVWAYEALVRGPNGEPAYTILDRVTDD
CCCCCCHHHHCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHCCC
TRYQFDQAARVKAIELAGTLFPRDSATRLSINFMPNAVYEPNACIRASLEAARRIDFAHR
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
RIMFEFTEQERFRDLEHVKGIVAAYRKQGFLTALDDFGAGFAGLSLLANFRPDLIKIDMD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCHHHHHHHHHHCCCCCEEECHH
VLRGLDNDAGRYAIVSGVVAIARALDVTVLAEGVETAGELDALRNIGITLVQGYHFAKPL
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHH
LEGLPPVAGFAPVAAARAA
HHCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]