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The map label for this gene is amtB [C]

Identifier: 170762016

GI number: 170762016

Start: 260694

End: 262217

Strand: Direct

Name: amtB [C]

Synonym: UPA3_0225

Alternate gene names: 170762016

Gene position: 260694-262217 (Clockwise)

Preceding gene: 170762079

Following gene: 170761982

Centisome position: 34.68

GC content: 34.12

Gene sequence:

>1524_bases
GTGTCAACAATTATAACTACATCTTGAGGAGTAGATCCAAATATTTACTTTTCAAAGCATGAGTTAACATGAGTGCCAGC
AACAGCTGATGCTAATACATTAGCATTAACTAATTTTAATCCGACTAGCACTTTATTAGTTGCATTAGCAATTGCCTTTG
TCTTATTGATGACTCCAGGTTTAGCATTATTTTATGGTGGGCTAACACGTCGAAAATCTACATTAACGATTATTAATCAA
TGTGTAGCTTCACTTGGAATCACAACACTGATTTGAATTTTTGGAGGGTTCTCACTTGCTTTTGGACCTTCTGTTGGTAA
AGGAATAATTGGTGATATTTCAACTTATTTTGCATTTAGAAATCTATTATTTGCTGATGGTTGAAGTGGAGATGTAGGAA
TTGTTTTTGCAAACTTTACTAATGGTGTACCATTAATTTTATTCTTTGCTTATCAATTAGCTTTTGCAATTATTACACCA
CCATTAATGGTTGGTGCATTTGCAGATCGAATGAAATTTAAAAATTATCTAGTATTCTTAGTACTATGACAATATTTAAT
TTACATTCCTTTTGCACATTGAATTTGAGGTCAAGGATTCTTAGCTGCTGCTGGAGTAATTGACTTTGCTGGTGGTATTG
TAATTCATACAACTGCTGGTTTTGGAGCATTAGCTGCTTCATTAGTATTAGGTAAACGTGTTTTACTTAAGAATGATAAA
AGTCGTCCACACAATATTCCAATGACTATTATTGGAGCAACATTGTTATTCTTTGGTTGATTTGGATTTAATGTTGGTGG
ATCAGGATTTGTAGCTGGTGGGAATGCTGCTACTTGATCAGCTGCTACAAGTGCTTGGATTAATACAATTATTGCTTTAG
CTATTGGAATGGTTGGTTGAGCAGTTCTTGAAACAATCTTTAATAAAAATCATAAAGCCACAGGTGTTGGTTTAGTAACA
GGTGCAATTGCTGGATTAGCAACAATTACTCCTGCAGCAGGTTATGTTCCAATTTGAGCATCTGTTCCTATTGGTGTAGC
TGCTGTGCTAGTTTGTTATACTAGTGCTAAAACTTTACATCATTTCCACAAAATTGATGATACTCTAGAAGTATGAGGCG
TACATGGAATGGGTGGTGTTACTGGTTCATTATTAATTGGTGCGTTTGCATCTAAATCTGTAAACCCATCAATTATGTAT
GAAGCAATTGGTCCAACAACAACTGGTGTTTTATTTGGTGTCCAATTAGGAGCGGTTTTATTAGCTGCGGTTTATGGATT
TGTATTTACTATTTTATTAGTATATATTACTCGTCCAAGATTATCAGCACGCCAACAATTAGGTCATATAGACTATATTA
ATCATGGCGAAGATGCATATTCATTCGATATTGAAATTCCATCTGAAAAAGAAATGGAGCAATATGAATTAGCAGATAGT
ACATGACACTCAAGCACAGGTGTTAAGTATTCATTGCTTAATAATAAAAACCAGCAAAATCATAAAGTAGCAAACGACCA
TTAA

Upstream 100 bases:

>100_bases
TGTGCTTGCTTTTTATTTTTTTAGTACTATAATAGTAATATGTACTTAAAAATTATATTTTGTTTTAAAGTATAAAAAAA
ATAAATAAGAGGTGAGAATT

Downstream 100 bases:

>100_bases
TTTTTAAAAAAAGACTAAAGCAAAATACTTTAGTTTTTTTAATTAATAAATAATAAAAAAATAAAGAATTTATTAATTAA
CGTAATATAATACTATAGAT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MSTIITTSWGVDPNIYFSKHELTWVPATADANTLALTNFNPTSTLLVALAIAFVLLMTPGLALFYGGLTRRKSTLTIINQ
CVASLGITTLIWIFGGFSLAFGPSVGKGIIGDISTYFAFRNLLFADGWSGDVGIVFANFTNGVPLILFFAYQLAFAIITP
PLMVGAFADRMKFKNYLVFLVLWQYLIYIPFAHWIWGQGFLAAAGVIDFAGGIVIHTTAGFGALAASLVLGKRVLLKNDK
SRPHNIPMTIIGATLLFFGWFGFNVGGSGFVAGGNAATWSAATSAWINTIIALAIGMVGWAVLETIFNKNHKATGVGLVT
GAIAGLATITPAAGYVPIWASVPIGVAAVLVCYTSAKTLHHFHKIDDTLEVWGVHGMGGVTGSLLIGAFASKSVNPSIMY
EAIGPTTTGVLFGVQLGAVLLAAVYGFVFTILLVYITRPRLSARQQLGHIDYINHGEDAYSFDIEIPSEKEMEQYELADS
TWHSSTGVKYSLLNNKNQQNHKVANDH

Sequences:

>Translated_507_residues
MSTIITTS*GVDPNIYFSKHELT*VPATADANTLALTNFNPTSTLLVALAIAFVLLMTPGLALFYGGLTRRKSTLTIINQ
CVASLGITTLI*IFGGFSLAFGPSVGKGIIGDISTYFAFRNLLFADG*SGDVGIVFANFTNGVPLILFFAYQLAFAIITP
PLMVGAFADRMKFKNYLVFLVL*QYLIYIPFAH*I*GQGFLAAAGVIDFAGGIVIHTTAGFGALAASLVLGKRVLLKNDK
SRPHNIPMTIIGATLLFFG*FGFNVGGSGFVAGGNAAT*SAATSAWINTIIALAIGMVG*AVLETIFNKNHKATGVGLVT
GAIAGLATITPAAGYVPI*ASVPIGVAAVLVCYTSAKTLHHFHKIDDTLEV*GVHGMGGVTGSLLIGAFASKSVNPSIMY
EAIGPTTTGVLFGVQLGAVLLAAVYGFVFTILLVYITRPRLSARQQLGHIDYINHGEDAYSFDIEIPSEKEMEQYELADS
T*HSSTGVKYSLLNNKNQQNHKVANDH
>Mature_506_residues
STIITTS*GVDPNIYFSKHELT*VPATADANTLALTNFNPTSTLLVALAIAFVLLMTPGLALFYGGLTRRKSTLTIINQC
VASLGITTLI*IFGGFSLAFGPSVGKGIIGDISTYFAFRNLLFADG*SGDVGIVFANFTNGVPLILFFAYQLAFAIITPP
LMVGAFADRMKFKNYLVFLVL*QYLIYIPFAH*I*GQGFLAAAGVIDFAGGIVIHTTAGFGALAASLVLGKRVLLKNDKS
RPHNIPMTIIGATLLFFG*FGFNVGGSGFVAGGNAAT*SAATSAWINTIIALAIGMVG*AVLETIFNKNHKATGVGLVTG
AIAGLATITPAAGYVPI*ASVPIGVAAVLVCYTSAKTLHHFHKIDDTLEV*GVHGMGGVTGSLLIGAFASKSVNPSIMYE
AIGPTTTGVLFGVQLGAVLLAAVYGFVFTILLVYITRPRLSARQQLGHIDYINHGEDAYSFDIEIPSEKEMEQYELADST
*HSSTGVKYSLLNNKNQQNHKVANDH

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=421, Percent_Identity=34.6793349168646, Blast_Score=187, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17550182, Length=452, Percent_Identity=27.6548672566372, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17550180, Length=450, Percent_Identity=24, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324187, Length=497, Percent_Identity=32.7967806841046, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321559, Length=488, Percent_Identity=27.2540983606557, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6325396, Length=464, Percent_Identity=26.7241379310345, Blast_Score=126, Evalue=9e-30,
Organism=Drosophila melanogaster, GI161078314, Length=413, Percent_Identity=24.6973365617433, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047
- InterPro:   IPR002229 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 51707; Mature: 51576

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIITTSGVDPNIYFSKHELTVPATADANTLALTNFNPTSTLLVALAIAFVLLMTPGLA
CCCEEECCCCCCCEEEECCEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCHH
LFYGGLTRRKSTLTIINQCVASLGITTLIIFGGFSLAFGPSVGKGIIGDISTYFAFRNLL
HHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHHHHHHE
FADGSGDVGIVFANFTNGVPLILFFAYQLAFAIITPPLMVGAFADRMKFKNYLVFLVLQY
EECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIYIPFAHIGQGFLAAAGVIDFAGGIVIHTTAGFGALAASLVLGKRVLLKNDKSRPHNIP
HHHCCHHHHCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHEECCCCCCCCCCC
MTIIGATLLFFGFGFNVGGSGFVAGGNAATSAATSAWINTIIALAIGMVGAVLETIFNKN
HHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HKATGVGLVTGAIAGLATITPAAGYVPIASVPIGVAAVLVCYTSAKTLHHFHKIDDTLEV
CCCCCHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
GVHGMGGVTGSLLIGAFASKSVNPSIMYEAIGPTTTGVLFGVQLGAVLLAAVYGFVFTIL
CCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVYITRPRLSARQQLGHIDYINHGEDAYSFDIEIPSEKEMEQYELADSTHSSTGVKYSLL
HHHHCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEE
NNKNQQNHKVANDH
CCCCCCCCCCCCCC
>Mature Secondary Structure 
STIITTSGVDPNIYFSKHELTVPATADANTLALTNFNPTSTLLVALAIAFVLLMTPGLA
CCEEECCCCCCCEEEECCEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCHH
LFYGGLTRRKSTLTIINQCVASLGITTLIIFGGFSLAFGPSVGKGIIGDISTYFAFRNLL
HHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHHHHHHE
FADGSGDVGIVFANFTNGVPLILFFAYQLAFAIITPPLMVGAFADRMKFKNYLVFLVLQY
EECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIYIPFAHIGQGFLAAAGVIDFAGGIVIHTTAGFGALAASLVLGKRVLLKNDKSRPHNIP
HHHCCHHHHCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHEECCCCCCCCCCC
MTIIGATLLFFGFGFNVGGSGFVAGGNAATSAATSAWINTIIALAIGMVGAVLETIFNKN
HHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HKATGVGLVTGAIAGLATITPAAGYVPIASVPIGVAAVLVCYTSAKTLHHFHKIDDTLEV
CCCCCHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
GVHGMGGVTGSLLIGAFASKSVNPSIMYEAIGPTTTGVLFGVQLGAVLLAAVYGFVFTIL
CCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LVYITRPRLSARQQLGHIDYINHGEDAYSFDIEIPSEKEMEQYELADSTHSSTGVKYSLL
HHHHCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEE
NNKNQQNHKVANDH
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]