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The map label for this gene is nrgA [H]

Identifier: 170761982

GI number: 170761982

Start: 262391

End: 263947

Strand: Direct

Name: nrgA [H]

Synonym: UPA3_0226

Alternate gene names: 170761982

Gene position: 262391-263947 (Clockwise)

Preceding gene: 170762016

Following gene: 170761980

Centisome position: 34.91

GC content: 33.2

Gene sequence:

>1557_bases
GTGTCAACTTGAGGCCTAAATAAAGATATTTATTTCTCAACTCATGAATTAACATGAGTGCCCGCAGATGCAAGTCAATC
AACACTTAATGCAATAGGTTTTAATCCTACTAATACACTACTAGTAGCTTTAGCAATTGCTTTTGTTTTACTAATGACAC
CAGGGTTAGCATTATTTTATGGTGGTTTAGTAAGACGTAAATCAGCTTTAACAATTATTAATCAATGTGTAGCTTCACTT
GGAATTACAACATTGATTTGAATTTTTGGAGGTTTCTCACTTGCTTTTGGACCTTCTGTTGGTAAAGGAATAATTGGTGA
TATTTCAACTTATTTTGCATTTAGAAATCTACTATTTGCTGATGGTTGAAGTGGAGGAGCAGTTTTAGTTTTTGCTAATT
TTACTAACGGCGTACCATTAATTTTATTCTTTGCTTATCAATTAGCTTTTGCAATTATTACACCACCATTAATGGTTGGT
GCATTTGCAGACCGAATGAAATTTAAAAATTATCTAGTATTCTTAGTACTATGACAATATTTAATTTATATTCCTTTTGC
ACATTGAATTTGAGGTCAAGGATTCTTAGCTGCTGCTGGAGTAATTGACTTTGCTGGTGGTATTGTAATTCATACAACTG
CTGGTTTTGGAGCTTTGGCTGCTTCATTTATGTTAGGAAAACGTACAACATTAATTACAGATAAAAAGCGAGCTAATAAT
TTACCAATGGTTGTATTAGGTGCAGCATTACTATTTTTTGGTTGATTTGGATTTAATGTTGGTGGTGCAGGTTTTGTTAA
AGAAAATACAAATCACATTTCACAAGCTATATCATCATGATTAAATACAATTATTGCCTTAGCAATAGGTATGATAGGTT
GAGTTACACTTGAAACTATTGTTAACAAAAACCACAAACCAACAACAGTAGGTTTAGTGACTGGTGCAGTCGCTGGATTA
GCAACAATTACTCCCGCAGCAGGTTTTGTTCCAGTTTGAGCCTCTGTTCCTATTGCCATTATGGGTGTTTTAGTATGTTA
TTCAGTTTCTAGATTATTAGATTACTTCCGTTTTGATGATTCTTTAGAAGTTCTACCAGTTCATGGAATGGGTGGTGTTA
TAGGTTCTTTATTAATTGGAGCATTTGCTACTAACACGGTTGCACATGGAATTAAATATGACGCCGTCGGATTAGATAAT
AATGGAAATCCAATTTATGGTATTTTATTTGGACTACAATTAGGTGCTGTATTATTAGCAGCAACATGGGCATTTGCCTT
TACATGTTTAATAGTTTATATTACTAAACCTCGTTTATCAGTGCGTCAACAATTAGGCCATATAGACTACATCAATCATG
GTGAAGATGCATACAAGTTTGATTTTATTATTCCTTCAGAAAGTGAAATGTTTGAATATGAAAGTTTTGATCAAACTTGA
GAATGTAAAGCTAGTGGTTGTAAAAAAAGCAACTTAGCTGCCAAGAAAAACCCTGTTTTTATAGAGAATGAGAATGATTG
TACATGACAATCTAAATCTCGTGAGTGTACAAAATAG

Upstream 100 bases:

>100_bases
TAATTAACGTAATATAATACTATAGATATATTTTGTATTTATAAATTTATGTACAAAATTTATTTATCGAAAACGAAAAA
GAAAACACGAGGTGAAAATT

Downstream 100 bases:

>100_bases
AAAATATCAGTATTTCATAATTAAGAAGTTAATTAAAACTAACTTCTTTTTTATTTATAATTTATAATTGATGAAATAGT
AATATTTATCTTAAATGGGG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSTWGLNKDIYFSTHELTWVPADASQSTLNAIGFNPTNTLLVALAIAFVLLMTPGLALFYGGLVRRKSALTIINQCVASL
GITTLIWIFGGFSLAFGPSVGKGIIGDISTYFAFRNLLFADGWSGGAVLVFANFTNGVPLILFFAYQLAFAIITPPLMVG
AFADRMKFKNYLVFLVLWQYLIYIPFAHWIWGQGFLAAAGVIDFAGGIVIHTTAGFGALAASFMLGKRTTLITDKKRANN
LPMVVLGAALLFFGWFGFNVGGAGFVKENTNHISQAISSWLNTIIALAIGMIGWVTLETIVNKNHKPTTVGLVTGAVAGL
ATITPAAGFVPVWASVPIAIMGVLVCYSVSRLLDYFRFDDSLEVLPVHGMGGVIGSLLIGAFATNTVAHGIKYDAVGLDN
NGNPIYGILFGLQLGAVLLAATWAFAFTCLIVYITKPRLSVRQQLGHIDYINHGEDAYKFDFIIPSESEMFEYESFDQTW
ECKASGCKKSNLAAKKNPVFIENENDCTWQSKSRECTK

Sequences:

>Translated_518_residues
MST*GLNKDIYFSTHELT*VPADASQSTLNAIGFNPTNTLLVALAIAFVLLMTPGLALFYGGLVRRKSALTIINQCVASL
GITTLI*IFGGFSLAFGPSVGKGIIGDISTYFAFRNLLFADG*SGGAVLVFANFTNGVPLILFFAYQLAFAIITPPLMVG
AFADRMKFKNYLVFLVL*QYLIYIPFAH*I*GQGFLAAAGVIDFAGGIVIHTTAGFGALAASFMLGKRTTLITDKKRANN
LPMVVLGAALLFFG*FGFNVGGAGFVKENTNHISQAISS*LNTIIALAIGMIG*VTLETIVNKNHKPTTVGLVTGAVAGL
ATITPAAGFVPV*ASVPIAIMGVLVCYSVSRLLDYFRFDDSLEVLPVHGMGGVIGSLLIGAFATNTVAHGIKYDAVGLDN
NGNPIYGILFGLQLGAVLLAATWAFAFTCLIVYITKPRLSVRQQLGHIDYINHGEDAYKFDFIIPSESEMFEYESFDQT*
ECKASGCKKSNLAAKKNPVFIENENDCT*QSKSRECTK
>Mature_517_residues
ST*GLNKDIYFSTHELT*VPADASQSTLNAIGFNPTNTLLVALAIAFVLLMTPGLALFYGGLVRRKSALTIINQCVASLG
ITTLI*IFGGFSLAFGPSVGKGIIGDISTYFAFRNLLFADG*SGGAVLVFANFTNGVPLILFFAYQLAFAIITPPLMVGA
FADRMKFKNYLVFLVL*QYLIYIPFAH*I*GQGFLAAAGVIDFAGGIVIHTTAGFGALAASFMLGKRTTLITDKKRANNL
PMVVLGAALLFFG*FGFNVGGAGFVKENTNHISQAISS*LNTIIALAIGMIG*VTLETIVNKNHKPTTVGLVTGAVAGLA
TITPAAGFVPV*ASVPIAIMGVLVCYSVSRLLDYFRFDDSLEVLPVHGMGGVIGSLLIGAFATNTVAHGIKYDAVGLDNN
GNPIYGILFGLQLGAVLLAATWAFAFTCLIVYITKPRLSVRQQLGHIDYINHGEDAYKFDFIIPSESEMFEYESFDQT*E
CKASGCKKSNLAAKKNPVFIENENDCT*QSKSRECTK

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=419, Percent_Identity=33.4128878281623, Blast_Score=181, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17550182, Length=436, Percent_Identity=27.0642201834862, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17550180, Length=448, Percent_Identity=22.9910714285714, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32564042, Length=360, Percent_Identity=24.7222222222222, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324187, Length=436, Percent_Identity=32.5688073394495, Blast_Score=177, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6325396, Length=489, Percent_Identity=26.7893660531697, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6321559, Length=352, Percent_Identity=29.5454545454545, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078314, Length=429, Percent_Identity=25.8741258741259, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 53478; Mature: 53347

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGLNKDIYFSTHELTVPADASQSTLNAIGFNPTNTLLVALAIAFVLLMTPGLALFYGG
CCCCCCCCEEEEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
LVRRKSALTIINQCVASLGITTLIIFGGFSLAFGPSVGKGIIGDISTYFAFRNLLFADGS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHHHHHHEEECCC
GGAVLVFANFTNGVPLILFFAYQLAFAIITPPLMVGAFADRMKFKNYLVFLVLQYLIYIP
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FAHIGQGFLAAAGVIDFAGGIVIHTTAGFGALAASFMLGKRTTLITDKKRANNLPMVVLG
HHHHCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCEEEECHHHCCCCCHHHHH
AALLFFGFGFNVGGAGFVKENTNHISQAISSLNTIIALAIGMIGVTLETIVNKNHKPTTV
HHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
GLVTGAVAGLATITPAAGFVPVASVPIAIMGVLVCYSVSRLLDYFRFDDSLEVLPVHGMG
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
GVIGSLLIGAFATNTVAHGIKYDAVGLDNNGNPIYGILFGLQLGAVLLAATWAFAFTCLI
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VYITKPRLSVRQQLGHIDYINHGEDAYKFDFIIPSESEMFEYESFDQTECKASGCKKSNL
HHHCCCCHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHHCCCCCCCHHHHHCCCCCCCC
AAKKNPVFIENENDCTQSKSRECTK
CCCCCCEEEECCCCHHCCCCCCCCC
>Mature Secondary Structure 
STGLNKDIYFSTHELTVPADASQSTLNAIGFNPTNTLLVALAIAFVLLMTPGLALFYGG
CCCCCCCEEEEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
LVRRKSALTIINQCVASLGITTLIIFGGFSLAFGPSVGKGIIGDISTYFAFRNLLFADGS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHHHHHHEEECCC
GGAVLVFANFTNGVPLILFFAYQLAFAIITPPLMVGAFADRMKFKNYLVFLVLQYLIYIP
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
FAHIGQGFLAAAGVIDFAGGIVIHTTAGFGALAASFMLGKRTTLITDKKRANNLPMVVLG
HHHHCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCEEEECHHHCCCCCHHHHH
AALLFFGFGFNVGGAGFVKENTNHISQAISSLNTIIALAIGMIGVTLETIVNKNHKPTTV
HHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
GLVTGAVAGLATITPAAGFVPVASVPIAIMGVLVCYSVSRLLDYFRFDDSLEVLPVHGMG
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
GVIGSLLIGAFATNTVAHGIKYDAVGLDNNGNPIYGILFGLQLGAVLLAATWAFAFTCLI
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VYITKPRLSVRQQLGHIDYINHGEDAYKFDFIIPSESEMFEYESFDQTECKASGCKKSNL
HHHCCCCHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHHCCCCCCCHHHHHCCCCCCCC
AAKKNPVFIENENDCTQSKSRECTK
CCCCCCEEEECCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]