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The map label for this gene is tyrS

Identifier: 170761932

GI number: 170761932

Start: 152620

End: 153867

Strand: Reverse

Name: tyrS

Synonym: UPA3_0123

Alternate gene names: 170761932

Gene position: 153867-152620 (Counterclockwise)

Preceding gene: 170762381

Following gene: 170762026

Centisome position: 20.47

GC content: 24.2

Gene sequence:

>1248_bases
ATGCATAGTTTAATTAAGGATTTAAAAGCACGAAACTTAATCAATAATATAACTAATGAAGAAAAATTAATAAAAGCTTT
AGAGAAAAATAAAGGAATTTATGTAGGTTTTGATCCTTCAGCTGATAGTTTACACTTAGGTAATTATATTATGATAATGT
TATTAAAACGCTTTCGTTTATATAACATTAAAACATTAGCGCTTGTTGGTGGTGCTACAGGAATGATTGGTGATCCAAGT
GGTAAATCCGCTGAACGTAATTTATTAGATAAAACAATTTTAGAAAAAAATATTGCAAAAATAAAATTTCAACTTGAAAA
ATTTACTAATTCTCAAGTTATTAATAATTATGTTTTTTATGAAAATATGACTTTTTTAGATTTTTTGCGTGATGTTGGAA
AGTTAATCAACATTAATTATTTATTAGAAAAAGAAATTATTAATTCTCGTTTATCTGTTGGTATTTCATATACAGAATTT
AGTTATAATTTGCTACAAGGTTATGATTTTTTACAATTATATAAAAATGATAACATAGCCATTCAAGCTGGTGGTAGTGA
TCAATGGGGTAACATTACAACTGGAATTGAAATGATTCGAAAAAATTTAGGTGATGACAATATCGCTTGTGGATTGACAA
TTAATTTATTAACAAATTCAGAAGGTAAAAAATTTGGTAAAAGTGAAAAAGGTGCTATTTATTTAGACGAAAATAAATCA
ACTGTTTATGAAATGTATCAGTTTTTAATTAATCAAAGCGATGCTGATGTAGAAAAATTACTTAATTTTTTAACATTAAT
TGATGTTGAAGAAATAAAAAAAATAATGCAAGCTCATAAAAATAATCCCGCTTTACGAGTTGCACAAAAAGCATTGGCTA
AAGCTGTTGTTGTTGATATACATGGACAACAAAAATATGAACAAGCATTACATATTAGCGAAGTACTATTTAATGGTAGT
ATTAGTACATTAAATCAAGAAGAATTAGAAATCGCAATTAAGAGTTTACCTGCAACAAAATTAGACAAAGATGAAATTAA
AATAATTGATTTATTAAATTTAGCTAATATTTCATCAAGTAACCGTATAGCACGAGATTTTTTAAATACAGGATCAATTT
TAATTAATGATATTAAAATAAATGATGAAAATTTTTTAGTCAAGAAACAAGATGCTATTAATCAAAAATTTAGTATTATT
AGAAAAGGTAAACGAAATTATTTTTTAATTTTATGAAATAAAGATTAA

Upstream 100 bases:

>100_bases
AATTATAAAACAAATCAATAATTTAATTTGATTTAAAGGTAATATAGTATTAAAATATATAATTATAAATCAAGTAATTA
ATTAAGGAAAAGATAGATCT

Downstream 100 bases:

>100_bases
AAAATTTAATCAATTTTATTTATAATATATATGTCATTACAATATTAAGTAGCGAACCTTGTCAGGCCAGAGATGGAGCA
GCAATAGCAATATCTTTTTA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MHSLIKDLKARNLINNITNEEKLIKALEKNKGIYVGFDPSADSLHLGNYIMIMLLKRFRLYNIKTLALVGGATGMIGDPS
GKSAERNLLDKTILEKNIAKIKFQLEKFTNSQVINNYVFYENMTFLDFLRDVGKLININYLLEKEIINSRLSVGISYTEF
SYNLLQGYDFLQLYKNDNIAIQAGGSDQWGNITTGIEMIRKNLGDDNIACGLTINLLTNSEGKKFGKSEKGAIYLDENKS
TVYEMYQFLINQSDADVEKLLNFLTLIDVEEIKKIMQAHKNNPALRVAQKALAKAVVVDIHGQQKYEQALHISEVLFNGS
ISTLNQEELEIAIKSLPATKLDKDEIKIIDLLNLANISSSNRIARDFLNTGSILINDIKINDENFLVKKQDAINQKFSII
RKGKRNYFLILWNKD

Sequences:

>Translated_415_residues
MHSLIKDLKARNLINNITNEEKLIKALEKNKGIYVGFDPSADSLHLGNYIMIMLLKRFRLYNIKTLALVGGATGMIGDPS
GKSAERNLLDKTILEKNIAKIKFQLEKFTNSQVINNYVFYENMTFLDFLRDVGKLININYLLEKEIINSRLSVGISYTEF
SYNLLQGYDFLQLYKNDNIAIQAGGSDQWGNITTGIEMIRKNLGDDNIACGLTINLLTNSEGKKFGKSEKGAIYLDENKS
TVYEMYQFLINQSDADVEKLLNFLTLIDVEEIKKIMQAHKNNPALRVAQKALAKAVVVDIHGQQKYEQALHISEVLFNGS
ISTLNQEELEIAIKSLPATKLDKDEIKIIDLLNLANISSSNRIARDFLNTGSILINDIKINDENFLVKKQDAINQKFSII
RKGKRNYFLIL*NKD
>Mature_415_residues
MHSLIKDLKARNLINNITNEEKLIKALEKNKGIYVGFDPSADSLHLGNYIMIMLLKRFRLYNIKTLALVGGATGMIGDPS
GKSAERNLLDKTILEKNIAKIKFQLEKFTNSQVINNYVFYENMTFLDFLRDVGKLININYLLEKEIINSRLSVGISYTEF
SYNLLQGYDFLQLYKNDNIAIQAGGSDQWGNITTGIEMIRKNLGDDNIACGLTINLLTNSEGKKFGKSEKGAIYLDENKS
TVYEMYQFLINQSDADVEKLLNFLTLIDVEEIKKIMQAHKNNPALRVAQKALAKAVVVDIHGQQKYEQALHISEVLFNGS
ISTLNQEELEIAIKSLPATKLDKDEIKIIDLLNLANISSSNRIARDFLNTGSILINDIKINDENFLVKKQDAINQKFSII
RKGKRNYFLIL*NKD

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=399, Percent_Identity=33.5839598997494, Blast_Score=225, Evalue=6e-59,
Organism=Escherichia coli, GI1787925, Length=422, Percent_Identity=41.7061611374408, Blast_Score=329, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI25145802, Length=341, Percent_Identity=34.0175953079179, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI25145805, Length=312, Percent_Identity=28.525641025641, Blast_Score=134, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6325160, Length=436, Percent_Identity=34.8623853211009, Blast_Score=256, Evalue=7e-69,
Organism=Drosophila melanogaster, GI20130383, Length=406, Percent_Identity=33.9901477832512, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46912; Mature: 46912

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSLIKDLKARNLINNITNEEKLIKALEKNKGIYVGFDPSADSLHLGNYIMIMLLKRFRL
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCEECCHHHHHHHHHHHHH
YNIKTLALVGGATGMIGDPSGKSAERNLLDKTILEKNIAKIKFQLEKFTNSQVINNYVFY
CCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCEEEE
ENMTFLDFLRDVGKLININYLLEKEIINSRLSVGISYTEFSYNLLQGYDFLQLYKNDNIA
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHCCCCEEEEEECCCEE
IQAGGSDQWGNITTGIEMIRKNLGDDNIACGLTINLLTNSEGKKFGKSEKGAIYLDENKS
EEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCH
TVYEMYQFLINQSDADVEKLLNFLTLIDVEEIKKIMQAHKNNPALRVAQKALAKAVVVDI
HHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEEE
HGQQKYEQALHISEVLFNGSISTLNQEELEIAIKSLPATKLDKDEIKIIDLLNLANISSS
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEHHHCCCCCC
NRIARDFLNTGSILINDIKINDENFLVKKQDAINQKFSIIRKGKRNYFLILNKD
CHHHHHHHCCCCEEEEEEEECCCCEEEECHHHHHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure
MHSLIKDLKARNLINNITNEEKLIKALEKNKGIYVGFDPSADSLHLGNYIMIMLLKRFRL
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCEECCHHHHHHHHHHHHH
YNIKTLALVGGATGMIGDPSGKSAERNLLDKTILEKNIAKIKFQLEKFTNSQVINNYVFY
CCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCEEEE
ENMTFLDFLRDVGKLININYLLEKEIINSRLSVGISYTEFSYNLLQGYDFLQLYKNDNIA
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHCCCCEEEEEECCCEE
IQAGGSDQWGNITTGIEMIRKNLGDDNIACGLTINLLTNSEGKKFGKSEKGAIYLDENKS
EEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCH
TVYEMYQFLINQSDADVEKLLNFLTLIDVEEIKKIMQAHKNNPALRVAQKALAKAVVVDI
HHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEEE
HGQQKYEQALHISEVLFNGSISTLNQEELEIAIKSLPATKLDKDEIKIIDLLNLANISSS
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEHHHCCCCCC
NRIARDFLNTGSILINDIKINDENFLVKKQDAINQKFSIIRKGKRNYFLILNKD
CHHHHHHHCCCCEEEEEEEECCCCEEEECHHHHHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA