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The map label for this gene is tilS

Identifier: 170761927

GI number: 170761927

Start: 93870

End: 94862

Strand: Reverse

Name: tilS

Synonym: UPA3_0076

Alternate gene names: 170761927

Gene position: 94862-93870 (Counterclockwise)

Preceding gene: 170762127

Following gene: 170762340

Centisome position: 12.62

GC content: 24.17

Gene sequence:

>993_bases
TTGATAAAGTTATGAACAAATTTAATTAATAGAATTAGTAATAAAAAATACTTAGCTGCTGTTTCTGGTGGTCCAGATTC
TATGGCAATGTTAAATATGTACAAAAGAAATATTCGTGTTGTTTGTCATGTGAATTATCATAAACGCGAATCATCCGATC
GTGATCAGCAAATAGTTGTCGATTTTTGTAGAAAAAATAATTTAATAATTGAAATTTTAGATGTTGATGAAAAAGTTTAT
GAACGTTATGGTCATATTGATAATTTTCAAACAAAAGCACGTTTAATTCGTTATGATTTTTTCAAAGAAATTGGTAAAAA
ATATAATATTGAAAATTTGTATATAGCGCATAATTTTGATGATTTTTTAGAAACGGCATATATGCAACGAGCTCGCCAAT
CAAAAGCCTTATTTTATGGCATTAAGGAAAGTAATGTTATTAATGGCATAATTGTTAAACGTCCGGTTTTATTTGTGCGT
AAACAAACATTACAACGCTATTGTGATGAAAATAAAATTAAGTATGGTATTGACGAAACAAATGAATTAGATATATACGA
ACGCAATCGTGTTCGTAAAATAATTAGTTCTTGAAGTTTAAATGAGGTTTATGATTTTAAAAAATTCGTTATTAAATATA
ATAAAGAACATAGTTCTTTTGCTAATTTTATAGATTTATCATATATTGAATTTAAAAAAAATAAATACAAATATGACTAC
TTTATTCGTCAAGACGATATGGTTCAATATTATTTAATTTACTACTTTTTAATTGATCATAAAATTATTAATCCAAGTGA
AAATAAAATCATTTCATTAATCAAATTCTTTGGTAAACAAATTAATAAAGAAAAAGCTTATCGTGTTCAAGAAAATATTT
ATATGCATGTTAATGATGATGAATTAATTAGTTTAATATCATACAATAAAAGCGATAATATTAGCAACTCTAATGTTGAT
ATTATCGATGATCATAATGTTATTGAGAAATAG

Upstream 100 bases:

>100_bases
CAATATGTCTTATCGAATTTTAAGATTGACGAATTAGTTATAATTAAACCAGCTATTATAAAGGGCGCTTTAGCTACATT
AGCAGCGATTGGTGAAGATT

Downstream 100 bases:

>100_bases
GTAGATAATAATTAATGGAAATTCGTGGTAAATTATTTGTTAGTCAAGTTGTTAGAAAGTTTAATTTAAACGTTGTTGCA
AATTCTGACTATATTGATCG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MIKLWTNLINRISNKKYLAAVSGGPDSMAMLNMYKRNIRVVCHVNYHKRESSDRDQQIVVDFCRKNNLIIEILDVDEKVY
ERYGHIDNFQTKARLIRYDFFKEIGKKYNIENLYIAHNFDDFLETAYMQRARQSKALFYGIKESNVINGIIVKRPVLFVR
KQTLQRYCDENKIKYGIDETNELDIYERNRVRKIISSWSLNEVYDFKKFVIKYNKEHSSFANFIDLSYIEFKKNKYKYDY
FIRQDDMVQYYLIYYFLIDHKIINPSENKIISLIKFFGKQINKEKAYRVQENIYMHVNDDELISLISYNKSDNISNSNVD
IIDDHNVIEK

Sequences:

>Translated_330_residues
MIKL*TNLINRISNKKYLAAVSGGPDSMAMLNMYKRNIRVVCHVNYHKRESSDRDQQIVVDFCRKNNLIIEILDVDEKVY
ERYGHIDNFQTKARLIRYDFFKEIGKKYNIENLYIAHNFDDFLETAYMQRARQSKALFYGIKESNVINGIIVKRPVLFVR
KQTLQRYCDENKIKYGIDETNELDIYERNRVRKIISS*SLNEVYDFKKFVIKYNKEHSSFANFIDLSYIEFKKNKYKYDY
FIRQDDMVQYYLIYYFLIDHKIINPSENKIISLIKFFGKQINKEKAYRVQENIYMHVNDDELISLISYNKSDNISNSNVD
IIDDHNVIEK
>Mature_330_residues
MIKL*TNLINRISNKKYLAAVSGGPDSMAMLNMYKRNIRVVCHVNYHKRESSDRDQQIVVDFCRKNNLIIEILDVDEKVY
ERYGHIDNFQTKARLIRYDFFKEIGKKYNIENLYIAHNFDDFLETAYMQRARQSKALFYGIKESNVINGIIVKRPVLFVR
KQTLQRYCDENKIKYGIDETNELDIYERNRVRKIISS*SLNEVYDFKKFVIKYNKEHSSFANFIDLSYIEFKKNKYKYDY
FIRQDDMVQYYLIYYFLIDHKIINPSENKIISLIKFFGKQINKEKAYRVQENIYMHVNDDELISLISYNKSDNISNSNVD
IIDDHNVIEK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 39375; Mature: 39375

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLTNLINRISNKKYLAAVSGGPDSMAMLNMYKRNIRVVCHVNYHKRESSDRDQQIVVD
CEEHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHHH
FCRKNNLIIEILDVDEKVYERYGHIDNFQTKARLIRYDFFKEIGKKYNIENLYIAHNFDD
HHCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHH
FLETAYMQRARQSKALFYGIKESNVINGIIVKRPVLFVRKQTLQRYCDENKIKYGIDETN
HHHHHHHHHHHHCCCEEEECCCCCCCCEEHECCHHHHHHHHHHHHHCCCCCEEECCCCCC
ELDIYERNRVRKIISSSLNEVYDFKKFVIKYNKEHSSFANFIDLSYIEFKKNKYKYDYFI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCEEEEEEE
RQDDMVQYYLIYYFLIDHKIINPSENKIISLIKFFGKQINKEKAYRVQENIYMHVNDDEL
CCCCHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHCCHHHHHEEECCEEEEECHHHH
ISLISYNKSDNISNSNVDIIDDHNVIEK
HHHHHCCCCCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MIKLTNLINRISNKKYLAAVSGGPDSMAMLNMYKRNIRVVCHVNYHKRESSDRDQQIVVD
CEEHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHHH
FCRKNNLIIEILDVDEKVYERYGHIDNFQTKARLIRYDFFKEIGKKYNIENLYIAHNFDD
HHCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHH
FLETAYMQRARQSKALFYGIKESNVINGIIVKRPVLFVRKQTLQRYCDENKIKYGIDETN
HHHHHHHHHHHHCCCEEEECCCCCCCCEEHECCHHHHHHHHHHHHHCCCCCEEECCCCCC
ELDIYERNRVRKIISSSLNEVYDFKKFVIKYNKEHSSFANFIDLSYIEFKKNKYKYDYFI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCEEEEEEE
RQDDMVQYYLIYYFLIDHKIINPSENKIISLIKFFGKQINKEKAYRVQENIYMHVNDDEL
CCCCHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHCCHHHHHEEECCEEEEECHHHH
ISLISYNKSDNISNSNVDIIDDHNVIEK
HHHHHCCCCCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]