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The map label for this gene is metG

Identifier: 170761906

GI number: 170761906

Start: 239846

End: 241411

Strand: Direct

Name: metG

Synonym: UPA3_0203

Alternate gene names: 170761906

Gene position: 239846-241411 (Clockwise)

Preceding gene: 170762441

Following gene: 170762047

Centisome position: 31.91

GC content: 25.54

Gene sequence:

>1566_bases
ATGTTAAAACAAAAGAAATTTTTTATCTCTACTCCCATTTACTATTCTTCTGGAAATCCTCATATTGGTCATGCTTATAC
AACGATCATTGCTGATGTTTTAGCACGTTATAAACGCTTGTTTGGATATGATGTTTTCTTTTTAACAGGAATGGATGAGC
ATGGTCAAAAAATCCAACAAAAAGCTTTTGAAGAAAACATTTCTCCCAAAGCTTTGGTCGATCGTAATTCAATTATTTTT
TTAAATTTATGAAAACGATTAAATATTAGTTTTTCAAAATTTATTCGTACAACGCAAATGGATCATGAAGAGTCTGTACA
AAAAGTTTTTAGTTATTTATATAAACAAGGAAAAATTTATTTAGGCCAATGGACAGGTTATTATTGTGTATCATGCGAAG
AAAATTATAATCCTGCTGAAATTATTAAAAGTCAAGATAATATAATGTTATGTCGCATGGGTCATAAATTAGAAACTAAG
TCTGAAGAATCATATTTTTATAAAATGAGTGATCAAGCACCATTTTTAAAAACTTACTATCAAAATCATCCGAATTTCAT
TATTCCAAATGAACGTGCAAATGAAATGGTTAATAACTTCTTAAATAATTTAGAAGATTTAAGTATTTCACGTACTACTT
TTGATTGAGGGATTCCTATTGCTGAAAATCCAAAACATGTAATTTATGTTTGATTAGATGCATTAATGAATTATTTAACT
GCAACTGGTTATTTATCAAATAATGAGGAATTATTTCAAAAATATTGATGTGATAATGAAACTGAAATTGTTCATTTATT
ATCAAAGGAAATTGCGCGTTTTCACTGCATTTATTGACCAATCTTTTTAAATGATTTACAAATTCGTTTTCCATCAACAA
TTTTATCACATGGATGAATCATTACAAAAGAAGGCAAAATGTCTAAATCATTAGGCAATGTAATTGATCCTAATGTTTTA
ATTGATACTTATGGTGTGGATGCACTTCGTTATTATTTAATGGCTGATTTAAGTTTATTTAGAGATGCTATTTTTAGTGA
AGATAATTTGATTGAAACTTATAATACTCAATTAGCAAATAGTTATGGTAACATGATTTCAAGAACATTGGGAATGTTAA
AAAAATATCGAAATAATATAGTTCCAAAATATGTTGGTTGTGTTTTAAAAAATGATGAAAAATTAGAAAATTTAATTAAT
AAAAATATTGAATTAGTTCAAGAAAACATTAATAAATATAGTATTGATAAAGCACTTAATTGCATTCAAGAAATTTTAGT
TGAAGCAAATAAATATATTGAAGATAATAAACCTTGAGAATTAGCTAAAAATCAACAAGAGCAAGAATTAGATTCACTTT
TAGTTCATTTAGTAAAAGTTATTCAAGTAACTACTACTTTATTATCACCAATTTTAATTGAAGGAAGTAAAAAAGCAGTT
GAACAATTAAATTTTGATGAATCTTTTTTAACTTTAGCTAGTTTAGCATCTTATGATATATTCAACTACCATAAAGTTAA
TGATTCAAAACCAATTTTTGCAAGAATCATTGTAGAAAAACAATAA

Upstream 100 bases:

>100_bases
AAAAAATATTATGTTCCCCAAAACAACGAACAAGAGTTTAAATTAAACCAAAATCAACAAAAATTTAAAAATAATAATTA
AAAATTTTAAGGAGAAAAAT

Downstream 100 bases:

>100_bases
AATCTTTAATTCTTTGTAATTATTAAAATAATTAAGCTTTTTTTATATAATTTATAAGACTAACATTTAATAGTAGTTGA
GGAATTAATGAGGTTAAGAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MLKQKKFFISTPIYYSSGNPHIGHAYTTIIADVLARYKRLFGYDVFFLTGMDEHGQKIQQKAFEENISPKALVDRNSIIF
LNLWKRLNISFSKFIRTTQMDHEESVQKVFSYLYKQGKIYLGQWTGYYCVSCEENYNPAEIIKSQDNIMLCRMGHKLETK
SEESYFYKMSDQAPFLKTYYQNHPNFIIPNERANEMVNNFLNNLEDLSISRTTFDWGIPIAENPKHVIYVWLDALMNYLT
ATGYLSNNEELFQKYWCDNETEIVHLLSKEIARFHCIYWPIFLNDLQIRFPSTILSHGWIITKEGKMSKSLGNVIDPNVL
IDTYGVDALRYYLMADLSLFRDAIFSEDNLIETYNTQLANSYGNMISRTLGMLKKYRNNIVPKYVGCVLKNDEKLENLIN
KNIELVQENINKYSIDKALNCIQEILVEANKYIEDNKPWELAKNQQEQELDSLLVHLVKVIQVTTTLLSPILIEGSKKAV
EQLNFDESFLTLASLASYDIFNYHKVNDSKPIFARIIVEKQ

Sequences:

>Translated_521_residues
MLKQKKFFISTPIYYSSGNPHIGHAYTTIIADVLARYKRLFGYDVFFLTGMDEHGQKIQQKAFEENISPKALVDRNSIIF
LNL*KRLNISFSKFIRTTQMDHEESVQKVFSYLYKQGKIYLGQWTGYYCVSCEENYNPAEIIKSQDNIMLCRMGHKLETK
SEESYFYKMSDQAPFLKTYYQNHPNFIIPNERANEMVNNFLNNLEDLSISRTTFD*GIPIAENPKHVIYV*LDALMNYLT
ATGYLSNNEELFQKY*CDNETEIVHLLSKEIARFHCIY*PIFLNDLQIRFPSTILSHG*IITKEGKMSKSLGNVIDPNVL
IDTYGVDALRYYLMADLSLFRDAIFSEDNLIETYNTQLANSYGNMISRTLGMLKKYRNNIVPKYVGCVLKNDEKLENLIN
KNIELVQENINKYSIDKALNCIQEILVEANKYIEDNKP*ELAKNQQEQELDSLLVHLVKVIQVTTTLLSPILIEGSKKAV
EQLNFDESFLTLASLASYDIFNYHKVNDSKPIFARIIVEKQ
>Mature_521_residues
MLKQKKFFISTPIYYSSGNPHIGHAYTTIIADVLARYKRLFGYDVFFLTGMDEHGQKIQQKAFEENISPKALVDRNSIIF
LNL*KRLNISFSKFIRTTQMDHEESVQKVFSYLYKQGKIYLGQWTGYYCVSCEENYNPAEIIKSQDNIMLCRMGHKLETK
SEESYFYKMSDQAPFLKTYYQNHPNFIIPNERANEMVNNFLNNLEDLSISRTTFD*GIPIAENPKHVIYV*LDALMNYLT
ATGYLSNNEELFQKY*CDNETEIVHLLSKEIARFHCIY*PIFLNDLQIRFPSTILSHG*IITKEGKMSKSLGNVIDPNVL
IDTYGVDALRYYLMADLSLFRDAIFSEDNLIETYNTQLANSYGNMISRTLGMLKKYRNNIVPKYVGCVLKNDEKLENLIN
KNIELVQENINKYSIDKALNCIQEILVEANKYIEDNKP*ELAKNQQEQELDSLLVHLVKVIQVTTTLLSPILIEGSKKAV
EQLNFDESFLTLASLASYDIFNYHKVNDSKPIFARIIVEKQ

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=517, Percent_Identity=30.174081237911, Blast_Score=223, Evalue=4e-58,
Organism=Homo sapiens, GI14043022, Length=564, Percent_Identity=24.113475177305, Blast_Score=122, Evalue=7e-28,
Organism=Escherichia coli, GI1788432, Length=549, Percent_Identity=24.5901639344262, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI212645096, Length=483, Percent_Identity=31.6770186335404, Blast_Score=204, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI71992781, Length=366, Percent_Identity=29.2349726775956, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17540794, Length=554, Percent_Identity=23.4657039711191, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321610, Length=505, Percent_Identity=30.6930693069307, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6321704, Length=572, Percent_Identity=23.2517482517483, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21358483, Length=492, Percent_Identity=26.6260162601626, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24655311, Length=560, Percent_Identity=23.9285714285714, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 59402; Mature: 59402

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQKKFFISTPIYYSSGNPHIGHAYTTIIADVLARYKRLFGYDVFFLTGMDEHGQKIQQ
CCCCCCEEEECCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHH
KAFEENISPKALVDRNSIIFLNLKRLNISFSKFIRTTQMDHEESVQKVFSYLYKQGKIYL
HHHHHCCCCCEEECCCCEEEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEE
GQWTGYYCVSCEENYNPAEIIKSQDNIMLCRMGHKLETKSEESYFYKMSDQAPFLKTYYQ
EECCCEEEEEECCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHC
NHPNFIIPNERANEMVNNFLNNLEDLSISRTTFDGIPIAENPKHVIYVLDALMNYLTATG
CCCCEEECCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHC
YLSNNEELFQKYCDNETEIVHLLSKEIARFHCIYPIFLNDLQIRFPSTILSHGIITKEGK
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHCCCCCCCCC
MSKSLGNVIDPNVLIDTYGVDALRYYLMADLSLFRDAIFSEDNLIETYNTQLANSYGNMI
HHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
SRTLGMLKKYRNNIVPKYVGCVLKNDEKLENLINKNIELVQENINKYSIDKALNCIQEIL
HHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
VEANKYIEDNKPELAKNQQEQELDSLLVHLVKVIQVTTTLLSPILIEGSKKAVEQLNFDE
HHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCH
SFLTLASLASYDIFNYHKVNDSKPIFARIIVEKQ
HHHHHHHHHHHCCCEEEECCCCCCEEEEEEEECC
>Mature Secondary Structure
MLKQKKFFISTPIYYSSGNPHIGHAYTTIIADVLARYKRLFGYDVFFLTGMDEHGQKIQQ
CCCCCCEEEECCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHH
KAFEENISPKALVDRNSIIFLNLKRLNISFSKFIRTTQMDHEESVQKVFSYLYKQGKIYL
HHHHHCCCCCEEECCCCEEEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEE
GQWTGYYCVSCEENYNPAEIIKSQDNIMLCRMGHKLETKSEESYFYKMSDQAPFLKTYYQ
EECCCEEEEEECCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHC
NHPNFIIPNERANEMVNNFLNNLEDLSISRTTFDGIPIAENPKHVIYVLDALMNYLTATG
CCCCEEECCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHC
YLSNNEELFQKYCDNETEIVHLLSKEIARFHCIYPIFLNDLQIRFPSTILSHGIITKEGK
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHCCCCCCCCC
MSKSLGNVIDPNVLIDTYGVDALRYYLMADLSLFRDAIFSEDNLIETYNTQLANSYGNMI
HHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
SRTLGMLKKYRNNIVPKYVGCVLKNDEKLENLINKNIELVQENINKYSIDKALNCIQEIL
HHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
VEANKYIEDNKPELAKNQQEQELDSLLVHLVKVIQVTTTLLSPILIEGSKKAVEQLNFDE
HHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCH
SFLTLASLASYDIFNYHKVNDSKPIFARIIVEKQ
HHHHHHHHHHHCCCEEEECCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]