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The map label for this gene is pncA [H]

Identifier: 170683713

GI number: 170683713

Start: 1407785

End: 1408426

Strand: Reverse

Name: pncA [H]

Synonym: EcSMS35_1423

Alternate gene names: 170683713

Gene position: 1408426-1407785 (Counterclockwise)

Preceding gene: 170682503

Following gene: 170679626

Centisome position: 27.79

GC content: 52.49

Gene sequence:

>642_bases
ATGCCCCCTCGCGCCCTGTTACTGGTCGATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCGCCGTGCCGGAAGGTGA
CAGTACGGTGGATGTCGCTAACCGCCTGATTGACTGGTGCCAGTCGCGCGGTGAAGCGGTTATCGCCAGTCAGGACTGGC
ATCCGGCGAATCACGGCAGTTTTGCCAGTCAGCACGGTGTAGAGCCTTATACGCCAGGCCAACTCGACGGTTTGCCACAA
ACCTTCTGGCCCGATCACTGTGTGCAGAACAGTGAAGGTGCACAATTACATCCGTTACTGAAGCAAAAAGCGATCGCAGC
GGTGTTCCATAAAGGCGAAAATCCTTTAGTTGACAGTTACAGTGCCTTTTTTGATAACGGCCGTCGGCAGAAAACTGCGC
TCGATGACTGGTTACGCGATCATGAAATCGATGAATTGATCATTATGGGCCTGGCTACTGACTATTGCGTGAAGTTTACC
GTGCTGGACGCGTTACAGTTAGGCTATAAGGTGAACGTGATTACCGATGGTTGTCGTGGCGTAAATATCCAGCCACAGGA
CAGTGCGCACGCGTTTATGGAAATGTCAGCAGCTGGGGCAACGCTATATACGCTGGCAGACTGGGAAGAAACGCAGGAGT
AA

Upstream 100 bases:

>100_bases
CATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGTGGCGAACTGACGCC
GGATGATTAAGGAGACTGCA

Downstream 100 bases:

>100_bases
TTTTACGCTGGCCTACAATTCTGTACTGGCATTGTAGGCCAGATAAAACACGTCAGTGGTACATCTGGCAATTGATGCCA
TCAACGAAAGATTTAATTCA

Product: nicotinamidase/pyrazinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRGEAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQ
TFWPDHCVQNSEGAQLHPLLKQKAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIIMGLATDYCVKFT
VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE

Sequences:

>Translated_213_residues
MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRGEAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQ
TFWPDHCVQNSEGAQLHPLLKQKAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIIMGLATDYCVKFT
VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE
>Mature_212_residues
PPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRGEAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQT
FWPDHCVQNSEGAQLHPLLKQKAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIIMGLATDYCVKFTV
LDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=212, Percent_Identity=98.5849056603774, Blast_Score=438, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI193206735, Length=218, Percent_Identity=37.6146788990826, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17543130, Length=218, Percent_Identity=37.6146788990826, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71999339, Length=211, Percent_Identity=30.8056872037915, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321401, Length=196, Percent_Identity=33.6734693877551, Blast_Score=117, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24648247, Length=205, Percent_Identity=36.0975609756098, Blast_Score=118, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 23423; Mature: 23292

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRGEAVIASQDWHPANHGS
CCCCEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
FASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQKAIAAVFHKGENPLVDSY
CHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCHHHH
SAFFDNGRRQKTALDDWLRDHEIDELIIMGLATDYCVKFTVLDALQLGYKVNVITDGCRG
HHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCC
VNIQPQDSAHAFMEMSAAGATLYTLADWEETQE
CCCCCCCHHHHHHHHHCCCCEEEEEECCHHCCC
>Mature Secondary Structure 
PPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRGEAVIASQDWHPANHGS
CCCEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
FASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQKAIAAVFHKGENPLVDSY
CHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCHHHH
SAFFDNGRRQKTALDDWLRDHEIDELIIMGLATDYCVKFTVLDALQLGYKVNVITDGCRG
HHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCC
VNIQPQDSAHAFMEMSAAGATLYTLADWEETQE
CCCCCCCHHHHHHHHHCCCCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]