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The map label for this gene is ycgG [H]

Identifier: 170683384

GI number: 170683384

Start: 2002860

End: 2004320

Strand: Reverse

Name: ycgG [H]

Synonym: EcSMS35_1983

Alternate gene names: 170683384

Gene position: 2004320-2002860 (Counterclockwise)

Preceding gene: 170680345

Following gene: 170684081

Centisome position: 39.55

GC content: 45.38

Gene sequence:

>1461_bases
TTGAACATCCAGCTCTGGTATTCCGCAAAAGCAGAGTACCTGGCAGGAGCGAGATATGCCGCCAACAATATCAATCATAT
ACTTGAAGAAGCGTCACAGGCGACTCAAACAGCGGTTAACATTGCCGGGAAGGAATGCGACCTCGAGGAGCAATATCAGC
TTGGCACTGAAGCAGCTCTGAAACCTCACCTGCGCACAATCATCATTCTCAAACAGGGAACGGTCTGGTGTACATCCTTA
CCGGGGAATCGGGTCCTGCTGTCTCGTATTCCTGTTTTCCCGGACAGTAATTTACTGTTGGCTCCGGCAATCGACACCGT
TAATAGATTACCTATCCTGCTCTATCAGAGCCAATTTGCAGATACGCGCATTTTGGTTACGATAAGTGATCAGCATATTC
GCGGGGCGCTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCTACGGGTGAT
GTGATGACGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCC
GCCACCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATATTGATATTTATTCTGTTAATCGCCTGCGCCG
CTGCTTTGCTGCTTGATAGGTATTTCAATAAAAGCGCAACGCCTGAAGAGATCCTGCGACGGGCTATAAATAATGGGGAG
ATCGTCCCTTTTTACCAACCTGTGGTAAATGGTCGGGAAGGGACATTGCGGGGAGTTGAGGTGTTAGCCCGCTGGAAACA
ACCTCACGGTGGATATATATCACCCGCGGCATTTATTCCACTTGCTGAAAAATCCGGATTAATCGTTCCGCTTACGCAAA
GCCTGATGAATCAGGTTGCCAGACAGATGAACGCTATCTCGAGTAAACTGCCGGAGGGTTTTCATATTGGAATTAATTTT
AGCGCCTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGCTTAAATTACCGTGACAGTTTTACCCGCCGCGATTTAAA
CCTCGTTCTGGAAGTCACCGAGCGTGAGCCATTAAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACATTCTGCATGAAA
ATGGTTTTGTCATCGCGCTGGATGATTTCGGTACTGGCTACTCAGGGCTTTCTTATCTGCATGACCTGCATATTGATTAT
ATCAAAATTGATCACAGTTTCGTTGGCCGCGTCAACGCAGACCCAGAATCAACCCGAATTCTGGATTGTGTATTGGATCT
GGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGGTGTCGAAACGAAAGAACAACTTGATTATCTGAACCAAAATAATA
TCACATTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAG
GTGAAGGTTGTGGTTGAGTGA

Upstream 100 bases:

>100_bases
ATTTTCATGTATTAAACCCTTTGGCCGGGACAAGCAAATGCGCAATACACTCATACCCATCCTCGTTGCGATATGCTTGT
TTATAACCGGTGTCGCTATT

Downstream 100 bases:

>100_bases
AAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTA
TTTTGTTTGCTATAATTGTT

Product: cyclic diguanylate phosphodiesterase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MNIQLWYSAKAEYLAGARYAANNINHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSL
PGNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNVPLKGVRYVLRVADDIIGPTGD
VMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLIDKGFGILIFILLIACAAALLLDRYFNKSATPEEILRRAINNGE
IVPFYQPVVNGREGTLRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAISSKLPEGFHIGINF
SASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNILHENGFVIALDDFGTGYSGLSYLHDLHIDY
IKIDHSFVGRVNADPESTRILDCVLDLARKLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPK
VKVVVE

Sequences:

>Translated_486_residues
MNIQLWYSAKAEYLAGARYAANNINHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSL
PGNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNVPLKGVRYVLRVADDIIGPTGD
VMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLIDKGFGILIFILLIACAAALLLDRYFNKSATPEEILRRAINNGE
IVPFYQPVVNGREGTLRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAISSKLPEGFHIGINF
SASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNILHENGFVIALDDFGTGYSGLSYLHDLHIDY
IKIDHSFVGRVNADPESTRILDCVLDLARKLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPK
VKVVVE
>Mature_486_residues
MNIQLWYSAKAEYLAGARYAANNINHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSL
PGNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNVPLKGVRYVLRVADDIIGPTGD
VMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLIDKGFGILIFILLIACAAALLLDRYFNKSATPEEILRRAINNGE
IVPFYQPVVNGREGTLRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAISSKLPEGFHIGINF
SASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNILHENGFVIALDDFGTGYSGLSYLHDLHIDY
IKIDHSFVGRVNADPESTRILDCVLDLARKLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPK
VKVVVE

Specific function: Unknown

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cytoplasm (Potential). Note=Found associated with the RNA degradosome [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI87081845, Length=486, Percent_Identity=98.3539094650206, Blast_Score=983, Evalue=0.0,
Organism=Escherichia coli, GI1786507, Length=248, Percent_Identity=44.758064516129, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788502, Length=252, Percent_Identity=39.2857142857143, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI87081980, Length=489, Percent_Identity=29.4478527607362, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI87081743, Length=293, Percent_Identity=34.1296928327645, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI87081921, Length=235, Percent_Identity=34.468085106383, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1790496, Length=279, Percent_Identity=31.8996415770609, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787541, Length=235, Percent_Identity=34.0425531914894, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI226510982, Length=250, Percent_Identity=31.2, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787055, Length=239, Percent_Identity=29.7071129707113, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI87082096, Length=235, Percent_Identity=29.7872340425532, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788849, Length=258, Percent_Identity=28.6821705426357, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001633 [H]

Pfam domain/function: PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 54582; Mature: 54582

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQLWYSAKAEYLAGARYAANNINHILEEASQATQTAVNIAGKECDLEEQYQLGTEAAL
CCEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHC
KPHLRTIIILKQGTVWCTSLPGNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFA
CCCEEEEEEEECCCEEEECCCCCEEEEEECCCCCCCCEEEECCHHHHCCCEEEEEECCCC
DTRILVTISDQHIRGALNVPLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYH
CEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEE
FTIIFNPPPLFSFYRLIDKGFGILIFILLIACAAALLLDRYFNKSATPEEILRRAINNGE
EEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
IVPFYQPVVNGREGTLRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVA
EEEEEHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHEEEECCCCCEEEEHHHHHHHHHH
RQMNAISSKLPEGFHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNV
HHHHHHHHHCCCCEEEEEECCHHHCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCC
DESLVQRLNILHENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRI
CHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCEEEEEECCCEECCCCCCCCHHHH
LDCVLDLARKLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPK
HHHHHHHHHHHEEEEEECCCCHHHHHHHCCCCCEEEECCEEEECCHHHHHHHHHHHCCCC
VKVVVE
EEEEEC
>Mature Secondary Structure
MNIQLWYSAKAEYLAGARYAANNINHILEEASQATQTAVNIAGKECDLEEQYQLGTEAAL
CCEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHC
KPHLRTIIILKQGTVWCTSLPGNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFA
CCCEEEEEEEECCCEEEECCCCCEEEEEECCCCCCCCEEEECCHHHHCCCEEEEEECCCC
DTRILVTISDQHIRGALNVPLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYH
CEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEE
FTIIFNPPPLFSFYRLIDKGFGILIFILLIACAAALLLDRYFNKSATPEEILRRAINNGE
EEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
IVPFYQPVVNGREGTLRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVA
EEEEEHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHEEEECCCCCEEEEHHHHHHHHHH
RQMNAISSKLPEGFHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNV
HHHHHHHHHCCCCEEEEEECCHHHCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCC
DESLVQRLNILHENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRI
CHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCEEEEEECCCEECCCCCCCCHHHH
LDCVLDLARKLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPK
HHHHHHHHHHHEEEEEECCCCHHHHHHHCCCCCEEEECCEEEECCHHHHHHHHHHHCCCC
VKVVVE
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]