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The map label for this gene is gltS [H]

Identifier: 170682521

GI number: 170682521

Start: 4065349

End: 4066554

Strand: Reverse

Name: gltS [H]

Synonym: EcSMS35_3988

Alternate gene names: 170682521

Gene position: 4066554-4065349 (Counterclockwise)

Preceding gene: 170680492

Following gene: 170681126

Centisome position: 80.23

GC content: 53.73

Gene sequence:

>1206_bases
ATGTTTCATCTCGATACTTTAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGCAAGTTGGTCCATTCCGT
CTCCTTTTTGAAGAAATACACCATACCGGAACCTGTTGCGGGTGGTTTATTAGTGGCGCTGGCGCTGCTGATACTGAAAA
AAAGCATGGGCTGGGAAGTCAACTTTGATATGTCCCTGCGCGATCCGTTGATGCTGGCTTTCTTCGCCACCATTGGCCTG
AACGCCAACATTGCCAGTTTGCGTGCCGGTGGGCGTGTGGTTGGCATCTTCTTGATTGTGGTTGTTGGCCTGTTGGTGAT
GCAAAATGCCATTGGCATTGGTATGGCTAGCCTGTTAGGGCTTGATCCGCTGATGGGGCTGTTGGCCGGTTCTATTACGC
TTTCCGGCGGTCACGGTACGGGAGCTGCATGGAGTAAATTGTTCATTGAACGTTATGGCTTCACCAATGCGACAGAAGTG
GCGATGGCCTGTGCAACGTTCGGTTTGGTGCTGGGCGGCTTGATTGGCGGTCCGGTGGCACGCTATCTGGTGAAACACTC
CACCACACCGAACGGTATTCCGGATGACCAGGAAGTTCCGACCGCGTTCGAAAAGCCGGATGTGGGCCGCATGATCACCT
CGCTGGTGTTGATTGAAACTATCGCGCTGATTGCTATCTGCCTGACGGTGGGGAAAATTGTTGCGCAACTTTTGGCTGGT
ACTGCTTTTGAACTACCGACCTTCGTCTGTGTACTATTTGTTGGCGTGATTCTGAGCAACGGTCTGTCAATGATGGGCTT
TTACCGCGTCTTTGAGCGAGCGGTATCCGTGCTGGGTAACGTAAGCCTGTCGTTGTTCCTGGCGATGGCGTTGATGGGGC
TGAAACTGTGGGAGCTGGCTTCGCTGGCGCTGCCGATGCTGGCGATTCTGGTGGTACAGACCATCTTCATGGCGTTGTAT
GCCATCTTCGTTACCTGGCGCATGATGGGCAAAAACTACGATGCGGCAGTGCTGGCTGCGGGTCACTGTGGCTTTGGCCT
TGGCGCAACGCCAACAGCAATCGCCAACATGCAGGCGATCACTGAACGCTTTGGCCCGTCGCACATGGCGTTCCTGGTAG
TGCCGATGGTCGGTGCGTTCTTTATCGATATCGTCAATGCGCTGGTGATTAAGCTGTATTTGATGTTGCCGATTTTTGCC
GGTTAA

Upstream 100 bases:

>100_bases
AGGAAAGCATGACTCGATACTCGTCATATTTCGCCTTACTGCAAATGCGCGATGAAGTATGACGAGTATGAAAGAGTGAT
GCGGACACAAAGGAGTAACT

Downstream 100 bases:

>100_bases
CCGATGAAGCGGCGGTATTCTCACCGCCGCCTTTTACGCATTCGAGTAACGTTCCGTCTCCGGCATCCAGCGTTCTATCA
GGGCTTTTGCCTGTTGTGGG

Product: sodium/glutamate symporter

Products: Na (I) [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate permease [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLLVALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGL
NANIASLRAGGRVVGIFLIVVVGLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLIETIALIAICLTVGKIVAQLLAG
TAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVSVLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALY
AIFVTWRMMGKNYDAAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKLYLMLPIFA
G

Sequences:

>Translated_401_residues
MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLLVALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGL
NANIASLRAGGRVVGIFLIVVVGLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLIETIALIAICLTVGKIVAQLLAG
TAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVSVLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALY
AIFVTWRMMGKNYDAAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKLYLMLPIFA
G
>Mature_401_residues
MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLLVALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGL
NANIASLRAGGRVVGIFLIVVVGLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLIETIALIAICLTVGKIVAQLLAG
TAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVSVLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALY
AIFVTWRMMGKNYDAAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKLYLMLPIFA
G

Specific function: Catalyzes the sodium-dependent uptake of extracellular glutamate [H]

COG id: COG0786

COG function: function code E; Na+/glutamate symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gltS symporter family [H]

Homologues:

Organism=Escherichia coli, GI1790085, Length=401, Percent_Identity=99.501246882793, Blast_Score=788, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004445 [H]

Pfam domain/function: PF03616 Glt_symporter [H]

EC number: NA

Molecular weight: Translated: 42458; Mature: 42458

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLLVALALLILKKSMGWEV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEE
NFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVVGLLVMQNAIGIGMASLLG
EECCCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEVAMACATFGLVLGGLIGGPVA
HHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLIETIALIAICLTVGKIVAQLLAG
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVSVLGNVSLSLFLAMALMGLKLWELA
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLALPMLAILVVQTIFMALYAIFVTWRMMGKNYDAAVLAAGHCGFGLGATPTAIANMQAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCCCCCCHHHHHHHHHH
TERFGPSHMAFLVVPMVGAFFIDIVNALVIKLYLMLPIFAG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLLVALALLILKKSMGWEV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEE
NFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVVGLLVMQNAIGIGMASLLG
EECCCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEVAMACATFGLVLGGLIGGPVA
HHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLIETIALIAICLTVGKIVAQLLAG
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVSVLGNVSLSLFLAMALMGLKLWELA
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLALPMLAILVVQTIFMALYAIFVTWRMMGKNYDAAVLAAGHCGFGLGATPTAIANMQAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCCCCCCHHHHHHHHHH
TERFGPSHMAFLVVPMVGAFFIDIVNALVIKLYLMLPIFAG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-glutamate [Periplasm] = Na (I) [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2254324; 2017136; 7686882; 9278503; 1544582 [H]