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The map label for this gene is ligB [H]

Identifier: 170681126

GI number: 170681126

Start: 4057512

End: 4059194

Strand: Reverse

Name: ligB [H]

Synonym: EcSMS35_3981

Alternate gene names: 170681126

Gene position: 4059194-4057512 (Counterclockwise)

Preceding gene: 170682521

Following gene: 170680501

Centisome position: 80.09

GC content: 52.53

Gene sequence:

>1683_bases
ATGAAAGTATGGATGGCGATATTAATAAGTATCTTGTGCTGGCAATCATCGGCGTGGGCAGTCTGTCCGGCCTGGTCGCC
AGCCAGAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAAATAAAACAGTGGGACGATGACTACTGGAAGGAAGGGAAAA
GTGAGGTGGAAGACGGTGTTTACGATCAGTTAAGCGCCCGTCTCACGCAGTGGCAACGCTGTTTTGGGAACGAGACTCGC
GACGCTATGATGCCGCCTTTAAACGGTGCGGTTATGCATCCGGTTGCCCATACGGGTGTACGCAAAATGGCGGATAAAAA
CGCATTAAGTCTGTGGATGCGCGAACGTAGCGATCTTTGGGTGCAGCCAAAAGTTGATGGCGTTGCGGTAACCCTGGTTT
ATCGGGACGGGAAACTGAACAAAGCAATCAGTCGCGGTAATGGCCTGAAAGGCGAGGACTGGACGCAGAAAGTTCGCTTA
ATTTCCGCTGTGCCGCAAACCGTTAGCGGGCCTTTAGCCAACAGCACGCTTCAGGGGGAAATATTTCTCAAGCGTAAGGG
GCATATCCAACAACAAATGGGGGGAATAAATGCCCGCGCAAAAGTTGCTGGCCTGATGATGCGCCAGGGGAATAGCGATA
CGCTAAACTCACTGGCTGTTTTTGTCTGGGCATGGCCGGATGGACCGCAGTTAATGACCGATCGTTTAAAGGAGCTGGCT
ACCGCAGGTTTTACTCTAACGCAGACGTATACCCGTGCGGTTAAAAATGCTGATGAGGTTGCGCACGTACGCAATGAGTG
GTGGAAAGCAAAATTACCCTTCGTCACCGATGGCGTGGTTGTGCGAGCGGCGAAAGAACCCGAATCCCGTCATTGGCTAC
CGGGCCAGGCGGAGTGGCTGGTGGCCTGGAAATATCAACCTGTAGCTCAGGTTGCCGAAGTGAAGGCGATTCAGTTTGCG
GTGGGTAAGAGCGGTAAAATATCGGTGGTTGCGTCACTCGCACCTGTCATGCTGGATGATAAAAAAGTCCAGCGGGTGAA
TATTGGTTCCGTCAGGCGCTGGCAGGAGTGGGATATTGCGCCTGGTGATCAGATCCTCGTCAGCCTTGCCGGTCAGGGTA
TTCCTCGCATTGATGATGTGGTCTGGCGCGGTGCAGAACGTACAAAACCGACACCACCAGAAAACCGCTTTAACTCGTTG
ACCTGCTACTTTGCTTCTGATGTTTGTCAGGAACAGTTCATTTCACGCTTAGTCTGGCTGGGGTCAAAACAGGTTCTTGG
GCTGGATGGCATTGGTGAGGCCGGCTGGCGCGCGCTGCATCAGACTCATCGCTTTGAGCATATCTTTTCCTGGCTTTTAT
TAACGCCAGAGCAATTACAGAACACGCCGGGGATCGCGAAAAGTAAAAGTGCGCAGTTATGGCATCAATTTAATCTGGCT
CGTCAGCAGCCTTTTACTCGCTGGGTGATGGCAATGGGAATACCGCTAACCCGGGCGGCGCTTAATGCCAGTGATGAACG
GTCCTGGTCGCAACTTTTATTTAGCACAGAACAGTTCTGGCAGCAGTTGCCGGGGACTGGATCGGGGCGCGCCAGACAGG
TTATTGAATGGAAGGAAAATGCGCAAATCAAGAAGCTGGGCAGTTGGCTGGCTGCCCAGCAGATCACAGGTTTTGAACCT
TAG

Upstream 100 bases:

>100_bases
GACGATTCTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGAC
TATCTACAGCGGAGGTGATG

Downstream 100 bases:

>100_bases
TGACCTTCGTGGCTGTAGTAAAAGACCGGTAATCCCAGCTTAAGACGTAGCGCCAGTAAGCGGGCAAAAAAGCCGAATAC
CAGGGTGGAGATAATCACCA

Product: NAD-dependent DNA ligase LigB

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] B [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETR
DAMMPPLNGAVMHPVAHTGVRKMADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
ISAVPQTVSGPLANSTLQGEIFLKRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSLAVFVWAWPDGPQLMTDRLKELA
TAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVTDGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFA
VGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSL
TCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLA
RQQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP

Sequences:

>Translated_560_residues
MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETR
DAMMPPLNGAVMHPVAHTGVRKMADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
ISAVPQTVSGPLANSTLQGEIFLKRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSLAVFVWAWPDGPQLMTDRLKELA
TAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVTDGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFA
VGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSL
TCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLA
RQQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP
>Mature_560_residues
MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETR
DAMMPPLNGAVMHPVAHTGVRKMADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
ISAVPQTVSGPLANSTLQGEIFLKRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSLAVFVWAWPDGPQLMTDRLKELA
TAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVTDGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFA
VGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSL
TCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLA
RQQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP

Specific function: Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction [H]

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082305, Length=560, Percent_Identity=96.9642857142857, Blast_Score=1112, Evalue=0.0,
Organism=Escherichia coli, GI1788750, Length=518, Percent_Identity=23.1660231660232, Blast_Score=102, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018239
- InterPro:   IPR020923
- InterPro:   IPR004150
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 63190; Mature: 63190

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVEDGV
CHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHH
YDQLSARLTQWQRCFGNETRDAMMPPLNGAVMHPVAHTGVRKMADKNALSLWMRERSDLW
HHHHHHHHHHHHHHHCCCCCHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCEE
VQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRLISAVPQTVSGPLANSTLQGE
ECCCCCCEEEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCE
IFLKRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSLAVFVWAWPDGPQLMTDRLKELA
EEEECCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHH
TAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVTDGVVVRAAKEPESRHWLPGQAEWL
HCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEECCEEEEECCCCCCCCCCCCCCCEE
VAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIA
EEEECCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCHHEEECCCCCCCCCCCCCC
PGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSLTCYFASDVCQEQFISRLVWL
CCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHC
GSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLA
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHH
RQQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKEN
HHCHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
AQIKKLGSWLAAQQITGFEP
CHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVEDGV
CHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHH
YDQLSARLTQWQRCFGNETRDAMMPPLNGAVMHPVAHTGVRKMADKNALSLWMRERSDLW
HHHHHHHHHHHHHHHCCCCCHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCEE
VQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRLISAVPQTVSGPLANSTLQGE
ECCCCCCEEEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCE
IFLKRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSLAVFVWAWPDGPQLMTDRLKELA
EEEECCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHH
TAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVTDGVVVRAAKEPESRHWLPGQAEWL
HCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEECCEEEEECCCCCCCCCCCCCCCEE
VAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIA
EEEECCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCHHEEECCCCCCCCCCCCCC
PGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSLTCYFASDVCQEQFISRLVWL
CCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHC
GSKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLA
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHH
RQQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKEN
HHCHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
AQIKKLGSWLAAQQITGFEP
CHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA