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The map label for this gene is rfaJ2 [H]

Identifier: 170679827

GI number: 170679827

Start: 4041135

End: 4042130

Strand: Reverse

Name: rfaJ2 [H]

Synonym: EcSMS35_3963

Alternate gene names: 170679827

Gene position: 4042130-4041135 (Counterclockwise)

Preceding gene: 170681283

Following gene: 170680583

Centisome position: 79.75

GC content: 35.24

Gene sequence:

>996_bases
ATGAATGAATTTATAAAAGAACGGTTTTCGTATTTAGCAGATAATAAAAAAGAAAACGTCCCAGAGCTAAATGTTTCCTA
CGGTATCGATAAGAATTTTTTGTATGGTGCTGGCGTTTCAATTTCTTCCGTTTTGATTAATAATTCAGATATTAATTTTG
TCTTTCATGTTTTCACTGATTATGTGGATGATGATTATTTAAAGTCATTTAATGAAACAGCAAAACAATTTAATACCTCA
ATTATTGTATATTTAATTGACCCAAAATACTTTGCTGATCTGCCGACGTCACAGTTTTGGTCGTACGCGACATACTTCAG
GGTATTGTCCTTTGAATATCTGAGTGAAAGTATTTCCACACTGCTGTATCTGGATGCCGATGTCGTTTGTAAAGGAAGTC
TGAAACCTCTCACAAAAATTATATTTAAAGATGAGTTTGCTGCGGTTATTCCTGACAATGATAGTACTCAGGCGGCATGT
GCAAAACGTCTTAACATTCCCGAAATGAATGGACGTTATTTCAATGCAGGCGTTATCTATGTCAATCTTAAAAAATGGCA
TGAAGCAAATTTGACACCGTATTTACTCACGCTTTTACGAGGGGAAACTAAATATGGCTCTCTTAAATATTTAGATCAGG
ATGCGTTGAATATCGCATTTAATATGAATAATATCTACCTCGCGAAGGATTTTGATACTATTTATACCCTGAAAAACGAA
CTTCATGATCGTAGTCATCGAAAGTATCAGCAAACCATTACCGATAAAACAGTATTGATTCACTATACAGGGATAACTAA
ACCATGGCATAGCTGGGCTGGATATCCGTCTGCATCATACTTTAATATCGCGCGTGAACAATCTCCCTGGAAGAAATATC
CTCTTAAAGAGGCGCGGACTGTTGCAGAAATGCAGAAACAATATAAGCATCTGTTTGCCCATGGTGAGTATATAAAAGGC
ATAACTTCATTAATTAAGTACAAGCTTAAGAAATAA

Upstream 100 bases:

>100_bases
GCCAAGCATAATTTTAAACAAAATAAACCAATTAACGGGATAATGAATTATATTTATTATTTTTATTTAAAGATAATAAA
ATGACATTGAGAAAAGTTAA

Downstream 100 bases:

>100_bases
ATTACTCTTATGATTACAAGTATACGTTACCGCGGCTTCTCATTTTATTACAAAAATAATGATAATAAATATAAAGAAAT
CTTTGATGAAATACTGGCCT

Product: lipopolysaccharide 1,2-glucosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNEFIKERFSYLADNKKENVPELNVSYGIDKNFLYGAGVSISSVLINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTS
IIVYLIDPKYFADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTKIIFKDEFAAVIPDNDSTQAAC
AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNE
LHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKG
ITSLIKYKLKK

Sequences:

>Translated_331_residues
MNEFIKERFSYLADNKKENVPELNVSYGIDKNFLYGAGVSISSVLINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTS
IIVYLIDPKYFADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTKIIFKDEFAAVIPDNDSTQAAC
AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNE
LHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKG
ITSLIKYKLKK
>Mature_331_residues
MNEFIKERFSYLADNKKENVPELNVSYGIDKNFLYGAGVSISSVLINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTS
IIVYLIDPKYFADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTKIIFKDEFAAVIPDNDSTQAAC
AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNE
LHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKG
ITSLIKYKLKK

Specific function: Adds the glucose(II) group on the galactose(I) group of LPS [H]

COG id: COG1442

COG function: function code M; Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 8 family [H]

Homologues:

Organism=Escherichia coli, GI1790056, Length=309, Percent_Identity=43.042071197411, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1790057, Length=332, Percent_Identity=31.6265060240964, Blast_Score=190, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002495
- InterPro:   IPR013645 [H]

Pfam domain/function: PF01501 Glyco_transf_8; PF08437 Glyco_transf_8N [H]

EC number: =2.4.1.58 [H]

Molecular weight: Translated: 38491; Mature: 38491

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEFIKERFSYLADNKKENVPELNVSYGIDKNFLYGAGVSISSVLINNSDINFVFHVFTD
CCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEECCCEEEEEEEECCCCEEEEEEHHH
YVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSYATYFRVLSFEYLSESIST
HCCHHHHHHHHHHHHHCCCEEEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLYLDADVVCKGSLKPLTKIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGRYFNAGVIY
EEEECCCEEECCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCCCCEEEECEEEE
VNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNE
EEEEECCCCCCCHHHHHHHCCCCCCCCEEEECCCCEEEEEECCEEEEEECCHHHHHHHHH
LHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEART
HHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHH
VAEMQKQYKHLFAHGEYIKGITSLIKYKLKK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNEFIKERFSYLADNKKENVPELNVSYGIDKNFLYGAGVSISSVLINNSDINFVFHVFTD
CCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEECCCEEEEEEEECCCCEEEEEEHHH
YVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSYATYFRVLSFEYLSESIST
HCCHHHHHHHHHHHHHCCCEEEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLYLDADVVCKGSLKPLTKIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGRYFNAGVIY
EEEECCCEEECCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCCCCEEEECEEEE
VNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNE
EEEEECCCCCCCHHHHHHHCCCCCCCCEEEECCCCEEEEEECCEEEEEECCHHHHHHHHH
LHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEART
HHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHH
VAEMQKQYKHLFAHGEYIKGITSLIKYKLKK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2235496; 9535865; 11677609 [H]