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The map label for this gene is rfaY [H]

Identifier: 170680583

GI number: 170680583

Start: 4040433

End: 4041125

Strand: Reverse

Name: rfaY [H]

Synonym: EcSMS35_3962

Alternate gene names: 170680583

Gene position: 4041125-4040433 (Counterclockwise)

Preceding gene: 170679827

Following gene: 170682179

Centisome position: 79.73

GC content: 35.35

Gene sequence:

>693_bases
ATGATTACAAGTATACGTTACCGCGGCTTCTCATTTTATTACAAAAATAATGATAATAAATATAAAGAAATCTTTGATGA
AATACTGGCCTATAATTTCAAAACAGTAAAAGTATTACGTAATATTGATGATACCAAAGTATCGTTGATTGATACGAAAT
ATGGTCGCTATGTTTTTAAAGTCTTTGCTCCGAAAACGAAAAGGAATGAACGTTTTTTAAAATCTTTTGTAAAAGGTGAC
TACTATCAGAATTTGATCGTTGAAACCGATCGTGTCAGAAGTGCAGGTCTTACTTTTCCTAATGATTTTTATTTTCTGGC
TGAGCGTAAAATTTTCAATTATGCCAGTGTTTTTATCATGCTTATCGAGTATGTCGAGGGCGTAGAGCTCAATGATATGC
CGATTATTCCAGAAGATGTTAAAGCAGAAATTAAGGCTTCAATGGAAAAATTGCATGCCTTAAATATGCTATCGGGCGAC
CCACACCGGGGTAACTTTATCGTTAGTAAAGATGGTGTCAGGATAATTGACCTATCCGGTAAAAGCTGTACGGCAGAGCG
TAAAGCTCGTGATCGTCTGGCCATGGAACGTCACCTGGGTATTGCCAACGAAATAAAAGATTATGGCTACTATTCAGTAA
TCTACAGAACCAAACTACGCAAATTTATTAAAAAATTAAAAGGCAAAGCGTAA

Upstream 100 bases:

>100_bases
AGAAATGCAGAAACAATATAAGCATCTGTTTGCCCATGGTGAGTATATAAAAGGCATAACTTCATTAATTAAGTACAAGC
TTAAGAAATAAATTACTCTT

Downstream 100 bases:

>100_bases
ACCACACAGTCAAAACGGAACCAACATGGATTTATTAGCTGAGAGTATTACTGAAGTCGCTGTCTCTGGGGAAATTGCTA
ACACCGATCGTGTGTTAAAT

Product: lipopolysaccharide core biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MITSIRYRGFSFYYKNNDNKYKEIFDEILAYNFKTVKVLRNIDDTKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGD
YYQNLIVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA

Sequences:

>Translated_230_residues
MITSIRYRGFSFYYKNNDNKYKEIFDEILAYNFKTVKVLRNIDDTKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGD
YYQNLIVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA
>Mature_230_residues
MITSIRYRGFSFYYKNNDNKYKEIFDEILAYNFKTVKVLRNIDDTKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGD
YYQNLIVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA

Specific function: Catalyzes the phosphorylation of heptose(II) of the outer membrane lipopolysaccharide core [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protein kinase superfamily. RfaY/waaY family [H]

Homologues:

Organism=Escherichia coli, GI1790055, Length=230, Percent_Identity=52.1739130434783, Blast_Score=258, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR009330 [H]

Pfam domain/function: PF06176 WaaY [H]

EC number: 2.7.-.-

Molecular weight: Translated: 26995; Mature: 26995

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSIRYRGFSFYYKNNDNKYKEIFDEILAYNFKTVKVLRNIDDTKVSLIDTKYGRYVFK
CCCEEEECCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCEEEE
VFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIM
EECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHH
LIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSG
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECC
KSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA
CCCCHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MITSIRYRGFSFYYKNNDNKYKEIFDEILAYNFKTVKVLRNIDDTKVSLIDTKYGRYVFK
CCCEEEECCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCEEEE
VFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIM
EECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHH
LIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSG
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECC
KSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA
CCCCHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA