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The map label for this gene is nudG [H]

Identifier: 170679665

GI number: 170679665

Start: 1417202

End: 1417609

Strand: Reverse

Name: nudG [H]

Synonym: EcSMS35_1430

Alternate gene names: 170679665

Gene position: 1417609-1417202 (Counterclockwise)

Preceding gene: 170684124

Following gene: 170681354

Centisome position: 27.97

GC content: 55.64

Gene sequence:

>408_bases
ATGAAAATGATTGAAGTTGTTGCCGCCATCATCGAACGTGATGGCAAAATTTTACTCGCGCAACGCCCCGCCCATAGCGA
TCAGGCGGGATTATGGGAGTTTGCCGGTGGTAAAGTCGAGCCGGATGAAAGCCAGCGGCAGGCGCTGGTGCGTGAGTTAC
GCGAAGAACTGGGCATCGAAGCAGCTGTGGGTGAATATGTTGCCAGCCATCAGCGAGAAGTTTCGGGGCGGATTATCCAT
CTTCATGCCTGGCACGTACCCGACTTCCACGGGACGTTACAGGCACATGAACATCAGGCGCTGGTCTGGTGCTCACCTGA
AGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCACTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAG
CGGATTAG

Upstream 100 bases:

>100_bases
TGGCGGTCTAGCACAGGCACTCCTTAAATATAAAGCCTTTCTGATTGAGCAACAGTGCGGATATTATGGCATTTTTCGCT
TATCTGCCCGTGTGTAATTT

Downstream 100 bases:

>100_bases
TGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGA
TCCTGATAAACGCAGCACTG

Product: pyrimidine (deoxy)nucleoside triphosphate pyrophosphohydrolase

Products: CMP; diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MKMIEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDESQRQALVRELREELGIEAAVGEYVASHQREVSGRIIH
LHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPADIPLLEAFMALRAARPAD

Sequences:

>Translated_135_residues
MKMIEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDESQRQALVRELREELGIEAAVGEYVASHQREVSGRIIH
LHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPADIPLLEAFMALRAARPAD
>Mature_135_residues
MKMIEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDESQRQALVRELREELGIEAAVGEYVASHQREVSGRIIH
LHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPADIPLLEAFMALRAARPAD

Specific function: Specific for pyrimidine substrates. Acts on 5-methyl- dCTP, CTP and dCTP in decreasing order [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=135, Percent_Identity=98.5185185185185, Blast_Score=267, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 15025; Mature: 15025

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMIEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDESQRQALVRELREELGIE
CHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCH
AAVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPADIP
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECHHHHHHCCCCCCCCH
LLEAFMALRAARPAD
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MKMIEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDESQRQALVRELREELGIE
CHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCH
AAVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPADIP
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECHHHHHHCCCCCCCCH
LLEAFMALRAARPAD
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; H2O [C]

Specific reaction: CTP + H2O = CMP + diphosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 11053429 [H]