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The map label for this gene is ksgA

Identifier: 170077519

GI number: 170077519

Start: 929212

End: 930033

Strand: Reverse

Name: ksgA

Synonym: SYNPCC7002_A0897

Alternate gene names: 170077519

Gene position: 930033-929212 (Counterclockwise)

Preceding gene: 170077520

Following gene: 170077515

Centisome position: 30.92

GC content: 51.7

Gene sequence:

>822_bases
ATGCGCCCCCGAAAACGGTTTGGTCAACATTGGCTGACGGATCAACAGATTTTAGATGAGATTGTTGCGGCGGCAAACCT
GCAACCGACAGACCGGGTCTTGGAAATTGGCCCAGGGAAAGGGGCGTTAACCAGCCGTTTATTGCCTCAAGTAGAAGCCT
TGCTCTCGGTAGAAATTGACCGGGATCTATGCAAGTACATGGTCAAAAATTATGGCGATCGCCCGAACTTTTTACTCCTC
GAAGCGGACTATCTCCAGACAGATATTAATGAATTTTTGGGGGATTTTCCCCAATTTCAGAACCCCCGCAAGGTGGTGGC
GAATATTCCCTACAACATCACCGGGCCGATTTTAGAGAAGCTCCTGGGCACCATTGACCGACCGAACTCGAATCCCTTTG
AGTCGATTGTGCTGCTGATTCAAAAGGAAGTGGGCGATCGCCTGGTAGCCCATCCCTGTACCAAAGCCTTTGGGGCCTTG
ACTCTGCGGGTGCAATATCTCGCCGATTGTGAAACGGTTTGTGTCGTCCCGCCGAAGGCCTTTTACCCGAAACCAAAGGT
CGAATCCGTGGTGGTGCGCCTACGGCCCCGGCCCCTTGCCCAGCCAGCCCAGAACCCCAAACTACTCGCAACCCTGATTA
AGGTGGGCTTTGCCAGTAAGCGCAAAATGCTGCGCAATAACCTTAAAAGCCTTTATAACCCGGAAATTCTCGACCCGATT
TTTGCGGATCTCGACATTAGTCCCCAGGCGAGGGGAGAAGAAGTGGATCTTTTACAATGGATTGCCCTGAGCGATCGCCT
CAATGATTACCCAAAGGAATAG

Upstream 100 bases:

>100_bases
CGGCGGTAGACCCGATGTTTATCTTGAGTGGGGAAAAAATTTAGGGGTAGAAAATGCTTGTAAAATAAACCCCTGCCCCT
GGATGGAGAGAGACAAAACG

Downstream 100 bases:

>100_bases
TGCTTTTTCACAGGTTGGGTAATCTCCCAGGTACAGGTCAGGCCCGCCGCAAAGGTCACTAAATCCCCTTTACCCATGGT
GACGGGGGAGCCGTCTTCTG

Product: S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MRPRKRFGQHWLTDQQILDEIVAAANLQPTDRVLEIGPGKGALTSRLLPQVEALLSVEIDRDLCKYMVKNYGDRPNFLLL
EADYLQTDINEFLGDFPQFQNPRKVVANIPYNITGPILEKLLGTIDRPNSNPFESIVLLIQKEVGDRLVAHPCTKAFGAL
TLRVQYLADCETVCVVPPKAFYPKPKVESVVVRLRPRPLAQPAQNPKLLATLIKVGFASKRKMLRNNLKSLYNPEILDPI
FADLDISPQARGEEVDLLQWIALSDRLNDYPKE

Sequences:

>Translated_273_residues
MRPRKRFGQHWLTDQQILDEIVAAANLQPTDRVLEIGPGKGALTSRLLPQVEALLSVEIDRDLCKYMVKNYGDRPNFLLL
EADYLQTDINEFLGDFPQFQNPRKVVANIPYNITGPILEKLLGTIDRPNSNPFESIVLLIQKEVGDRLVAHPCTKAFGAL
TLRVQYLADCETVCVVPPKAFYPKPKVESVVVRLRPRPLAQPAQNPKLLATLIKVGFASKRKMLRNNLKSLYNPEILDPI
FADLDISPQARGEEVDLLQWIALSDRLNDYPKE
>Mature_273_residues
MRPRKRFGQHWLTDQQILDEIVAAANLQPTDRVLEIGPGKGALTSRLLPQVEALLSVEIDRDLCKYMVKNYGDRPNFLLL
EADYLQTDINEFLGDFPQFQNPRKVVANIPYNITGPILEKLLGTIDRPNSNPFESIVLLIQKEVGDRLVAHPCTKAFGAL
TLRVQYLADCETVCVVPPKAFYPKPKVESVVVRLRPRPLAQPAQNPKLLATLIKVGFASKRKMLRNNLKSLYNPEILDPI
FADLDISPQARGEEVDLLQWIALSDRLNDYPKE

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=234, Percent_Identity=34.6153846153846, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI156415992, Length=277, Percent_Identity=28.158844765343, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1786236, Length=267, Percent_Identity=34.0823970037453, Blast_Score=156, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25146882, Length=192, Percent_Identity=36.4583333333333, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI25141369, Length=294, Percent_Identity=25.5102040816327, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324989, Length=237, Percent_Identity=31.2236286919831, Blast_Score=96, Evalue=5e-21,
Organism=Drosophila melanogaster, GI21358017, Length=236, Percent_Identity=34.7457627118644, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21357273, Length=289, Percent_Identity=26.2975778546713, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_SYNP2 (B1XIV9)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001734157.1
- ProteinModelPortal:   B1XIV9
- SMR:   B1XIV9
- GeneID:   6056556
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A0897
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30892; Mature: 30892

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 10-10 BINDING 12-12 BINDING 37-37 BINDING 58-58 BINDING 83-83 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRKRFGQHWLTDQQILDEIVAAANLQPTDRVLEIGPGKGALTSRLLPQVEALLSVEID
CCCHHHHHHHCCCHHHHHHHHHHHCCCCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHH
RDLCKYMVKNYGDRPNFLLLEADYLQTDINEFLGDFPQFQNPRKVVANIPYNITGPILEK
HHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHH
LLGTIDRPNSNPFESIVLLIQKEVGDRLVAHPCTKAFGALTLRVQYLADCETVCVVPPKA
HHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
FYPKPKVESVVVRLRPRPLAQPAQNPKLLATLIKVGFASKRKMLRNNLKSLYNPEILDPI
CCCCCCHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FADLDISPQARGEEVDLLQWIALSDRLNDYPKE
HHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRPRKRFGQHWLTDQQILDEIVAAANLQPTDRVLEIGPGKGALTSRLLPQVEALLSVEID
CCCHHHHHHHCCCHHHHHHHHHHHCCCCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHH
RDLCKYMVKNYGDRPNFLLLEADYLQTDINEFLGDFPQFQNPRKVVANIPYNITGPILEK
HHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHH
LLGTIDRPNSNPFESIVLLIQKEVGDRLVAHPCTKAFGALTLRVQYLADCETVCVVPPKA
HHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
FYPKPKVESVVVRLRPRPLAQPAQNPKLLATLIKVGFASKRKMLRNNLKSLYNPEILDPI
CCCCCCHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FADLDISPQARGEEVDLLQWIALSDRLNDYPKE
HHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA