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The map label for this gene is murD [H]

Identifier: 170077472

GI number: 170077472

Start: 876764

End: 877960

Strand: Reverse

Name: murD [H]

Synonym: SYNPCC7002_A0850

Alternate gene names: 170077472

Gene position: 877960-876764 (Counterclockwise)

Preceding gene: 170077473

Following gene: 170077471

Centisome position: 29.19

GC content: 53.63

Gene sequence:

>1197_bases
ATGGTCAAACTCGGCCATACCCCCAGTTTGAAAGAAGGCGATCGCCCGGATTTAATCGTCGTCAGTCCGGGGGTGCCCTG
GGATATTCCTTTTCTGAAAGAGGCGCGGGATCAAGGCATTGACACCATCGGCGAAATGGAATTGGCCTGGCGCAATCTGG
ATCAAAAACCCTGGGTCGGCATTACAGGCACCAATGGTAAAACCACCACTACGGCCCTGGTAGAAGCGATTTTTCAGGTA
GCGGGTCGGGGCGGCCCTGCCTGCGGGAATATTGGCCATGCCGCCTGCGAGCTTGCGTTGGGAGAAGCCACCCCGGAGTG
GATCGTGGCCGAAATTAGTAGCTATCAGATCGAATCTGCGCCGACCCTGCGTCCCCAAATTGGTGTGTGGACGACCTTCA
CGCCAGACCACCTCGCCCGCCACTACACCCTCGACAATTATCACAGCATTAAAGCGTCCCTCATTCAGCGTAGTCGTCGC
CAGGTCTTAAATGGAGATGATCCGTTTTTGCGATCGCAGGCAGAGCAATGGCCCGACGCCTATTGGACAAGCATCAAGGG
AGCAGAACATTTACCGTGCGATCCTAAACGGGGGGTATTTATCCAGGATGCTTGGGTGAAAGCCTTTGGGGAATTGGTAA
TTCCGGTTTCTCTACTCAAAATGGTCGGCGACCATAATCTCCAGAATTTACTCTTGGCGATCGCCGCCGCCCGGTTAGCA
AATATTGACAAAGGGGCGATCGCCGAGGCTATTGCTACTTTTCCTGGGGTGCCCCATCGTCTTGAGTTCATTTGCCAGCA
CCAGGGGGTTGATTTTATCAACGACAGTAAAGCAACGAACTATGATGCTGCTGAGGTGGGCTTAAATGCGGCCCCTGGCC
CGGTGATTCTCATTGCTGGGGGCGAACCCAAAGCAGGCAATGCTGGGGCCTGGATCGAAAAAATTAAAGAAAAGGCAGCG
ACGGTGCTTTTAATTGGTGAGGCGGCCCCCCAGTTTGCTCAGTTACTCCAAGAGAGCGATTACACCCAGTACGAAATCGT
TGAAACCCTAGAAAAGGCGATCGCCCGCAGTGTTGCCTTAATTCCGACCTATCAACCGAAAACGGTGCTTCTTTCTCCCG
CCTGTGCCAGCTTCGATCAATACCGCAGTTTCGAGCACCGAGGCGATCATTTTCGTCACCTTTGCCAAGCTCTATAG

Upstream 100 bases:

>100_bases
ACGGCTTTTAAAGCGGCAAGGTTGGGAGGTGGTCTTAGGCGATCGCCAAACGGATGAATCCCTCGTGGCAGTGCAACAGG
AGCTGTCGGCCCAAGGGATT

Downstream 100 bases:

>100_bases
AGCCAAAACCCTTTAGCATTAAAGAGAATTGCAAACACCTACTGGGAGCGCACTGTGAACCAAAGGCCCCTAGCCATTGA
TTTGTTTGCCGGATGCGGCG

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MVKLGHTPSLKEGDRPDLIVVSPGVPWDIPFLKEARDQGIDTIGEMELAWRNLDQKPWVGITGTNGKTTTTALVEAIFQV
AGRGGPACGNIGHAACELALGEATPEWIVAEISSYQIESAPTLRPQIGVWTTFTPDHLARHYTLDNYHSIKASLIQRSRR
QVLNGDDPFLRSQAEQWPDAYWTSIKGAEHLPCDPKRGVFIQDAWVKAFGELVIPVSLLKMVGDHNLQNLLLAIAAARLA
NIDKGAIAEAIATFPGVPHRLEFICQHQGVDFINDSKATNYDAAEVGLNAAPGPVILIAGGEPKAGNAGAWIEKIKEKAA
TVLLIGEAAPQFAQLLQESDYTQYEIVETLEKAIARSVALIPTYQPKTVLLSPACASFDQYRSFEHRGDHFRHLCQAL

Sequences:

>Translated_398_residues
MVKLGHTPSLKEGDRPDLIVVSPGVPWDIPFLKEARDQGIDTIGEMELAWRNLDQKPWVGITGTNGKTTTTALVEAIFQV
AGRGGPACGNIGHAACELALGEATPEWIVAEISSYQIESAPTLRPQIGVWTTFTPDHLARHYTLDNYHSIKASLIQRSRR
QVLNGDDPFLRSQAEQWPDAYWTSIKGAEHLPCDPKRGVFIQDAWVKAFGELVIPVSLLKMVGDHNLQNLLLAIAAARLA
NIDKGAIAEAIATFPGVPHRLEFICQHQGVDFINDSKATNYDAAEVGLNAAPGPVILIAGGEPKAGNAGAWIEKIKEKAA
TVLLIGEAAPQFAQLLQESDYTQYEIVETLEKAIARSVALIPTYQPKTVLLSPACASFDQYRSFEHRGDHFRHLCQAL
>Mature_398_residues
MVKLGHTPSLKEGDRPDLIVVSPGVPWDIPFLKEARDQGIDTIGEMELAWRNLDQKPWVGITGTNGKTTTTALVEAIFQV
AGRGGPACGNIGHAACELALGEATPEWIVAEISSYQIESAPTLRPQIGVWTTFTPDHLARHYTLDNYHSIKASLIQRSRR
QVLNGDDPFLRSQAEQWPDAYWTSIKGAEHLPCDPKRGVFIQDAWVKAFGELVIPVSLLKMVGDHNLQNLLLAIAAARLA
NIDKGAIAEAIATFPGVPHRLEFICQHQGVDFINDSKATNYDAAEVGLNAAPGPVILIAGGEPKAGNAGAWIEKIKEKAA
TVLLIGEAAPQFAQLLQESDYTQYEIVETLEKAIARSVALIPTYQPKTVLLSPACASFDQYRSFEHRGDHFRHLCQAL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=388, Percent_Identity=35.3092783505155, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 43448; Mature: 43448

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKLGHTPSLKEGDRPDLIVVSPGVPWDIPFLKEARDQGIDTIGEMELAWRNLDQKPWVG
CCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEE
ITGTNGKTTTTALVEAIFQVAGRGGPACGNIGHAACELALGEATPEWIVAEISSYQIESA
EECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHEECCC
PTLRPQIGVWTTFTPDHLARHYTLDNYHSIKASLIQRSRRQVLNGDDPFLRSQAEQWPDA
CCCCCCCCEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHH
YWTSIKGAEHLPCDPKRGVFIQDAWVKAFGELVIPVSLLKMVGDHNLQNLLLAIAAARLA
HHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NIDKGAIAEAIATFPGVPHRLEFICQHQGVDFINDSKATNYDAAEVGLNAAPGPVILIAG
CCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEEEC
GEPKAGNAGAWIEKIKEKAATVLLIGEAAPQFAQLLQESDYTQYEIVETLEKAIARSVAL
CCCCCCCCHHHHHHHHHHHCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEE
IPTYQPKTVLLSPACASFDQYRSFEHRGDHFRHLCQAL
ECCCCCCEEEECCHHHCHHHHHHHHHCCHHHHHHHHHC
>Mature Secondary Structure
MVKLGHTPSLKEGDRPDLIVVSPGVPWDIPFLKEARDQGIDTIGEMELAWRNLDQKPWVG
CCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEE
ITGTNGKTTTTALVEAIFQVAGRGGPACGNIGHAACELALGEATPEWIVAEISSYQIESA
EECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHEECCC
PTLRPQIGVWTTFTPDHLARHYTLDNYHSIKASLIQRSRRQVLNGDDPFLRSQAEQWPDA
CCCCCCCCEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHH
YWTSIKGAEHLPCDPKRGVFIQDAWVKAFGELVIPVSLLKMVGDHNLQNLLLAIAAARLA
HHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NIDKGAIAEAIATFPGVPHRLEFICQHQGVDFINDSKATNYDAAEVGLNAAPGPVILIAG
CCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEEEC
GEPKAGNAGAWIEKIKEKAATVLLIGEAAPQFAQLLQESDYTQYEIVETLEKAIARSVAL
CCCCCCCCHHHHHHHHHHHCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEE
IPTYQPKTVLLSPACASFDQYRSFEHRGDHFRHLCQAL
ECCCCCCEEEECCHHHCHHHHHHHHHCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA