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The map label for this gene is dacB [H]

Identifier: 170077438

GI number: 170077438

Start: 841805

End: 843244

Strand: Reverse

Name: dacB [H]

Synonym: SYNPCC7002_A0816

Alternate gene names: 170077438

Gene position: 843244-841805 (Counterclockwise)

Preceding gene: 170077441

Following gene: 170077437

Centisome position: 28.03

GC content: 51.53

Gene sequence:

>1440_bases
ATGGGGAAAACACAATTTCAACCGGTAAGCCAGATCCTCGCCCTGGCAAGTTTAGCAACCCTGGCTTTTAGCTCCCAAAG
TCTGGCCCAATCCCAAGAGACCTGCGATCTCTCTGGCACTATTTCTCAGATCCTCCAGCGTCCAGAACAGCAACAAACCC
AGTGGGGCATTGCGATCCAAGATCCCCAGACCGATAGCCTCTTATTTGAGCACCGAGCCGACACTTTTTTTGTGCCAGCC
TCTAACCAAAAACTCCTCACCACTGCCGCTGCCCTGGTAAGTTTTGGCCCAAACTATCAATATCAAACGCCAATCTACAC
CGTTGGAACAGCTCCCAATCTTGAGAAATTAATTATTCGAGGTGTGGGTGATCCAAGTTTGACCACCGAAAAATTAACCA
CAGCTCTCTCGCAGCTATCCCAACAGCAAATCCACCATATTGATCAAGTCATCCTGGACAGCAGCCATTTTCCTGAACCT
GGGGTTAATCCTACCTGGGAATGGTCTGATCTGAGTTTTTATTATGGTGTCCCCGTTACCAGCTTGATGCTCAATGAAAA
CGCCGTTGTTCTCACGGTTTTTCCCACAGAGTCCGGCCAAACCACAACGGTGGGCTGGTCTGATGATATTGCGGCCCAAC
AATGGCAACTGGAAAATCAGATGGTGACGGCCCCTGACGATACTCCCTACAGCGGTTCCCTGACCCAAACCTATGGCACG
ACGACGTTAACCCTCACCGGGAAATTGGCCGCCGACGCCATGCCCGACATTTGGGGACTATCGATTCCCGATCCAGACCG
CTATGCCCTCGATAAAATCACCCAAATTCTGGGAGAGCAGGGTATTACCGTCGGGAAAAGTTTAACGACGCAAACGCCGA
TCCCTAACCTAGATCAAGCGCAACCTTTGGGGGCGATCGCCTCTGATCCCTTAGCTGAAATCCTCAAAACCACTAACCAA
GACAGCAATAACCTTTTTGCCGAAAGTCTCCTAAAAACCCTTACTGCCGCTGACCGAGACCCAGAAACAATCCTAGAATC
TATCGGGGTCGATCCTGCCACCTACCGACTCCGGGATGGCTCTGGTCTTTCTCGCCACAACCTTGTCACCCCCATGGCCT
TGATCCAAACCCTAGACGGTATGCTGACCCATCCCCAGGGGGAAACTTACCGCAATTCTTTGGCGATCGCCGGTCGCACA
GGCACTCTCCAAAACCGCTTTCAAGATACCCCAGTCGAAAACCAATTCTTTGGGAAAACAGGCACCATGACCCACGTTTC
TGCCCTCTCTGGCTACCTCGATCTACCCGATGACAGCACCTTATTGGTCAGTATTTTGGCGAACCAAACCGGCCAATCGG
CCCGAATTACCCGTCAAGCCATTGATGATGTTGTCGTCGCTGTCCATGATTGGCACTCCTGTTCCACGACCCAGCCATAA

Upstream 100 bases:

>100_bases
CCAAGAAAACCACAAAGATCCTTTTCGCAGTGTACCGAAGCCCACCAAAAACGGCAGTATAGTAGGACAAATGCGCCCTG
ACCGTTGACGAGGAGAAAAA

Downstream 100 bases:

>100_bases
CCTCCGCTGTTGATCGAATACAAGGCGAATATTCAATTTTTCCGATTCAAGTTGCAACAATGACCCCAGACGCCATCTCC
GCTACCCTCACTGAATTTTT

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 4a; PBP-4a [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MGKTQFQPVSQILALASLATLAFSSQSLAQSQETCDLSGTISQILQRPEQQQTQWGIAIQDPQTDSLLFEHRADTFFVPA
SNQKLLTTAAALVSFGPNYQYQTPIYTVGTAPNLEKLIIRGVGDPSLTTEKLTTALSQLSQQQIHHIDQVILDSSHFPEP
GVNPTWEWSDLSFYYGVPVTSLMLNENAVVLTVFPTESGQTTTVGWSDDIAAQQWQLENQMVTAPDDTPYSGSLTQTYGT
TTLTLTGKLAADAMPDIWGLSIPDPDRYALDKITQILGEQGITVGKSLTTQTPIPNLDQAQPLGAIASDPLAEILKTTNQ
DSNNLFAESLLKTLTAADRDPETILESIGVDPATYRLRDGSGLSRHNLVTPMALIQTLDGMLTHPQGETYRNSLAIAGRT
GTLQNRFQDTPVENQFFGKTGTMTHVSALSGYLDLPDDSTLLVSILANQTGQSARITRQAIDDVVVAVHDWHSCSTTQP

Sequences:

>Translated_479_residues
MGKTQFQPVSQILALASLATLAFSSQSLAQSQETCDLSGTISQILQRPEQQQTQWGIAIQDPQTDSLLFEHRADTFFVPA
SNQKLLTTAAALVSFGPNYQYQTPIYTVGTAPNLEKLIIRGVGDPSLTTEKLTTALSQLSQQQIHHIDQVILDSSHFPEP
GVNPTWEWSDLSFYYGVPVTSLMLNENAVVLTVFPTESGQTTTVGWSDDIAAQQWQLENQMVTAPDDTPYSGSLTQTYGT
TTLTLTGKLAADAMPDIWGLSIPDPDRYALDKITQILGEQGITVGKSLTTQTPIPNLDQAQPLGAIASDPLAEILKTTNQ
DSNNLFAESLLKTLTAADRDPETILESIGVDPATYRLRDGSGLSRHNLVTPMALIQTLDGMLTHPQGETYRNSLAIAGRT
GTLQNRFQDTPVENQFFGKTGTMTHVSALSGYLDLPDDSTLLVSILANQTGQSARITRQAIDDVVVAVHDWHSCSTTQP
>Mature_478_residues
GKTQFQPVSQILALASLATLAFSSQSLAQSQETCDLSGTISQILQRPEQQQTQWGIAIQDPQTDSLLFEHRADTFFVPAS
NQKLLTTAAALVSFGPNYQYQTPIYTVGTAPNLEKLIIRGVGDPSLTTEKLTTALSQLSQQQIHHIDQVILDSSHFPEPG
VNPTWEWSDLSFYYGVPVTSLMLNENAVVLTVFPTESGQTTTVGWSDDIAAQQWQLENQMVTAPDDTPYSGSLTQTYGTT
TLTLTGKLAADAMPDIWGLSIPDPDRYALDKITQILGEQGITVGKSLTTQTPIPNLDQAQPLGAIASDPLAEILKTTNQD
SNNLFAESLLKTLTAADRDPETILESIGVDPATYRLRDGSGLSRHNLVTPMALIQTLDGMLTHPQGETYRNSLAIAGRTG
TLQNRFQDTPVENQFFGKTGTMTHVSALSGYLDLPDDSTLLVSILANQTGQSARITRQAIDDVVVAVHDWHSCSTTQP

Specific function: Catalyzes DD-carboxypeptidase and transpeptidation reactions [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Secreted (Probable). Note=May be anchored in the membrane via a C-terminal amphiphatic alpha helix [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=386, Percent_Identity=24.3523316062176, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 51991; Mature: 51859

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTQFQPVSQILALASLATLAFSSQSLAQSQETCDLSGTISQILQRPEQQQTQWGIAIQ
CCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHCCEEEC
DPQTDSLLFEHRADTFFVPASNQKLLTTAAALVSFGPNYQYQTPIYTVGTAPNLEKLIIR
CCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEECCCEEEECCCCCHHHHHEE
GVGDPSLTTEKLTTALSQLSQQQIHHIDQVILDSSHFPEPGVNPTWEWSDLSFYYGVPVT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEECCCEE
SLMLNENAVVLTVFPTESGQTTTVGWSDDIAAQQWQLENQMVTAPDDTPYSGSLTQTYGT
EEEECCCEEEEEEEECCCCCEEEECCCCCHHHHHHHHCCCEEECCCCCCCCCCEEEECCC
TTLTLTGKLAADAMPDIWGLSIPDPDRYALDKITQILGEQGITVGKSLTTQTPIPNLDQA
EEEEEECCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
QPLGAIASDPLAEILKTTNQDSNNLFAESLLKTLTAADRDPETILESIGVDPATYRLRDG
CCCCCHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECC
SGLSRHNLVTPMALIQTLDGMLTHPQGETYRNSLAIAGRTGTLQNRFQDTPVENQFFGKT
CCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCEEEECCCCCHHHHHCCCCCCCCCCCCC
GTMTHVSALSGYLDLPDDSTLLVSILANQTGQSARITRQAIDDVVVAVHDWHSCSTTQP
CCHHHHHHHHCCEECCCCCHHHHEEHHCCCCCHHHHHHHHHHHHEEEEECCCCCCCCCC
>Mature Secondary Structure 
GKTQFQPVSQILALASLATLAFSSQSLAQSQETCDLSGTISQILQRPEQQQTQWGIAIQ
CCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHCCEEEC
DPQTDSLLFEHRADTFFVPASNQKLLTTAAALVSFGPNYQYQTPIYTVGTAPNLEKLIIR
CCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCEECCCEEEECCCCCHHHHHEE
GVGDPSLTTEKLTTALSQLSQQQIHHIDQVILDSSHFPEPGVNPTWEWSDLSFYYGVPVT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEECCCEE
SLMLNENAVVLTVFPTESGQTTTVGWSDDIAAQQWQLENQMVTAPDDTPYSGSLTQTYGT
EEEECCCEEEEEEEECCCCCEEEECCCCCHHHHHHHHCCCEEECCCCCCCCCCEEEECCC
TTLTLTGKLAADAMPDIWGLSIPDPDRYALDKITQILGEQGITVGKSLTTQTPIPNLDQA
EEEEEECCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
QPLGAIASDPLAEILKTTNQDSNNLFAESLLKTLTAADRDPETILESIGVDPATYRLRDG
CCCCCHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECC
SGLSRHNLVTPMALIQTLDGMLTHPQGETYRNSLAIAGRTGTLQNRFQDTPVENQFFGKT
CCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCEEEECCCCCHHHHHCCCCCCCCCCCCC
GTMTHVSALSGYLDLPDDSTLLVSILANQTGQSARITRQAIDDVVVAVHDWHSCSTTQP
CCHHHHHHHHCCEECCCCCHHHHEEHHCCCCCHHHHHHHHHHHHEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7711903; 9384377 [H]