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The map label for this gene is dut [H]

Identifier: 170026364

GI number: 170026364

Start: 4588479

End: 4588937

Strand: Direct

Name: dut [H]

Synonym: YPK_4157

Alternate gene names: 170026364

Gene position: 4588479-4588937 (Clockwise)

Preceding gene: 229597228

Following gene: 170026365

Centisome position: 97.85

GC content: 49.02

Gene sequence:

>459_bases
ATGATGAAAAAAATCGACATTAAAATTCTGGACCCACGTGTTGGCAACGAATTTCCATTACCCACGTATGCCACCGAAGG
TTCTGCCGGGCTGGATTTACGTGCTTGTTTAGATCACGCAGTAGAACTACAGCCTGGACAAACAACCTTGTTACCAACCG
GTTTAGCCATTCACATTGGTGACAGTGCCCTCGCCGCGGTTATTTTGCCGCGTTCAGGTCTGGGGCATAAACACGGGATC
GTATTAGGTAATTTAGTGGGCCTTATAGACTCCGACTATCAGGGGCAGTTGATGGTATCCGTATGGAACCGTGGCCAGCA
GCCTTTCACCATCGAACCCGGTGAGCGTATCGCTCAGATGGTATTTGTTCCTGTGGTACAAGCAGAGTTCAATCTGGTTG
AAGACTTTACCGACAGTGAACGTGGTACCGGTGGTTTTGGCCATTCTGGACGCCAATAA

Upstream 100 bases:

>100_bases
AATGCCTTGCACCTTTTTTGGTCGACTGGAGAGAAACATTTACCACTCAGTGATAAATATCTTCTCAGTCAACGTTTAAT
AGACGAGATTGTCAGCCGTT

Downstream 100 bases:

>100_bases
CCAACCCTAGACCCCAATACCGTAAAAAAGCTATCACATAAGCCGCCTGGGCATTGTTCCCAGAGCGGCCGGTGTTTGGG
TTTTTGTTATTAAGTCATTT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MMKKIDIKILDPRVGNEFPLPTYATEGSAGLDLRACLDHAVELQPGQTTLLPTGLAIHIGDSALAAVILPRSGLGHKHGI
VLGNLVGLIDSDYQGQLMVSVWNRGQQPFTIEPGERIAQMVFVPVVQAEFNLVEDFTDSERGTGGFGHSGRQ

Sequences:

>Translated_152_residues
MMKKIDIKILDPRVGNEFPLPTYATEGSAGLDLRACLDHAVELQPGQTTLLPTGLAIHIGDSALAAVILPRSGLGHKHGI
VLGNLVGLIDSDYQGQLMVSVWNRGQQPFTIEPGERIAQMVFVPVVQAEFNLVEDFTDSERGTGGFGHSGRQ
>Mature_152_residues
MMKKIDIKILDPRVGNEFPLPTYATEGSAGLDLRACLDHAVELQPGQTTLLPTGLAIHIGDSALAAVILPRSGLGHKHGI
VLGNLVGLIDSDYQGQLMVSVWNRGQQPFTIEPGERIAQMVFVPVVQAEFNLVEDFTDSERGTGGFGHSGRQ

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI70906444, Length=140, Percent_Identity=33.5714285714286, Blast_Score=84, Evalue=5e-17,
Organism=Homo sapiens, GI4503423, Length=140, Percent_Identity=33.5714285714286, Blast_Score=84, Evalue=6e-17,
Organism=Homo sapiens, GI70906441, Length=140, Percent_Identity=33.5714285714286, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1790071, Length=151, Percent_Identity=84.7682119205298, Blast_Score=269, Evalue=7e-74,
Organism=Caenorhabditis elegans, GI71988561, Length=132, Percent_Identity=37.1212121212121, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319729, Length=132, Percent_Identity=32.5757575757576, Blast_Score=72, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583610, Length=149, Percent_Identity=32.8859060402685, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921126, Length=131, Percent_Identity=32.824427480916, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 16344; Mature: 16344

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKIDIKILDPRVGNEFPLPTYATEGSAGLDLRACLDHAVELQPGQTTLLPTGLAIHIG
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEECCCCCEEEECCCEEEEEC
DSALAAVILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMVSVWNRGQQPFTIEPGERIAQM
CCCEEEEEECCCCCCCCCCEEEHHHHHHCCCCCCCEEEEEEECCCCCCEEECCHHHHHHH
VFVPVVQAEFNLVEDFTDSERGTGGFGHSGRQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMKKIDIKILDPRVGNEFPLPTYATEGSAGLDLRACLDHAVELQPGQTTLLPTGLAIHIG
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEECCCCCEEEECCCEEEEEC
DSALAAVILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMVSVWNRGQQPFTIEPGERIAQM
CCCEEEEEECCCCCCCCCCEEEHHHHHHCCCCCCCEEEEEEECCCCCCEEECCHHHHHHH
VFVPVVQAEFNLVEDFTDSERGTGGFGHSGRQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA