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The map label for this gene is imm [H]

Identifier: 170026257

GI number: 170026257

Start: 4466149

End: 4466406

Strand: Reverse

Name: imm [H]

Synonym: YPK_4047

Alternate gene names: 170026257

Gene position: 4466406-4466149 (Counterclockwise)

Preceding gene: 170026258

Following gene: 170026248

Centisome position: 95.24

GC content: 34.5

Gene sequence:

>258_bases
ATGGAACTTAAAGAGAAGTATGAGGATTATACAGAGCATGAATTTTTAGAGTTTATTAGAAATATTTGTGAGGTGAATAC
TGATAGTCAATCTTTGCATAGTTCATGGGTGAGACACTTCACAAAAATCACTGAGCATCCGTCAGGATCAGATTTAATTT
ATTATCCAGAAGATGGTGCAGATGATAGTCCAGAAGGAATACTTGAGTTAGTAAAAAAATGGCGAGCTGAAAATGGTAAG
CCCGGTTTCAAAAAATAA

Upstream 100 bases:

>100_bases
TCGATCAAAAGTGGTGGAGCAGTTTATGATGTGGATAATTTACGAGTAGCAACGCCAAAACGCCACATTGAAATTCATTC
AAGACGTGGAGGAAAATAAA

Downstream 100 bases:

>100_bases
ATAAAATATATAATCCAAACCGCCTTCTGGTGGTTTTGTTAATATTTATCTGCATTAAAATAACAATTCACTCATAATTT
TGATGCTAATTTCATCTCAA

Product: colicin immunity protein/pyocin immunity protein

Products: NA

Alternate protein names: ImmE9; Microcin-E9 immunity protein [H]

Number of amino acids: Translated: 85; Mature: 85

Protein sequence:

>85_residues
MELKEKYEDYTEHEFLEFIRNICEVNTDSQSLHSSWVRHFTKITEHPSGSDLIYYPEDGADDSPEGILELVKKWRAENGK
PGFKK

Sequences:

>Translated_85_residues
MELKEKYEDYTEHEFLEFIRNICEVNTDSQSLHSSWVRHFTKITEHPSGSDLIYYPEDGADDSPEGILELVKKWRAENGK
PGFKK
>Mature_85_residues
MELKEKYEDYTEHEFLEFIRNICEVNTDSQSLHSSWVRHFTKITEHPSGSDLIYYPEDGADDSPEGILELVKKWRAENGK
PGFKK

Specific function: This protein is able to protect a cell, which harbors the plasmid ColE9 encoding colicin E9, against colicin E9, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the colicins ColE2/ColE8/ColE9 and pyocins S1/S2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000290
- ProDom:   PD007225 [H]

Pfam domain/function: PF01320 Colicin_Pyocin [H]

EC number: NA

Molecular weight: Translated: 9991; Mature: 9991

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKEKYEDYTEHEFLEFIRNICEVNTDSQSLHSSWVRHFTKITEHPSGSDLIYYPEDGA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC
DDSPEGILELVKKWRAENGKPGFKK
CCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MELKEKYEDYTEHEFLEFIRNICEVNTDSQSLHSSWVRHFTKITEHPSGSDLIYYPEDGA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC
DDSPEGILELVKKWRAENGKPGFKK
CCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2549375; 3312476; 8755730 [H]