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The map label for this gene is rep [H]

Identifier: 170026248

GI number: 170026248

Start: 4454995

End: 4457016

Strand: Reverse

Name: rep [H]

Synonym: YPK_4038

Alternate gene names: 170026248

Gene position: 4457016-4454995 (Counterclockwise)

Preceding gene: 170026257

Following gene: 170026245

Centisome position: 95.04

GC content: 48.76

Gene sequence:

>2022_bases
ATGCGATTAAACCCCAGCCAACAAGAAGCCGTCGAATTTGTCACTGGACCCTGTCTGGTGCTGGCCGGTGCGGGATCGGG
TAAAACCCGAGTCATTACCAACAAGATTGCCCACCTGATCCGTCAGTGTGGTTATCAGCCTAAGCATATTGCTGCCGTGA
CCTTCACCAATAAAGCAGCCCGCGAGATGAAAGAGCGTGTTGCTCAAACGCTGGGGCGCAAAGAAGCGCGGGGGTTGATG
ATCGCGACTTTCCACACCTTGGGGTTGGAAATCATCAAGAAAGAGTATGTGGCTCTGGGGATGAAGTCGAACTTCTCACT
GTTCGATGCACAGGATCAAATGGGGTTATTGAAAGATCTGACCCATAAGTGGCTGGAAGATGACAAAACATTGCTGCAGC
AACTGGTGTCGGCGATCTCCAATTGGAAGAATGATCTGCTGGATCCAGCCGCCGCTGCTGCGACAGCACGATCCGAGCGT
GATAAGTTATTTGTCCATTGCTACGGCTTATATGACGCCCATTTGAAAGCCTGTAATGTGCTGGATTTTGACGACCTTAT
CTCGCTACCCACCCTATTGTTGCAAAAAAATCTGGAGGTCCGTGAGCGGTGGCAAAACCGTCTGCGTTACCTACTGGTTG
ATGAATATCAGGATACCAATACCAGCCAGTATCAAATGGTGAAATTGCTGGTGGGGAATCGGGCGCGTTTTACTGTCGTG
GGTGATGACGACCAATCTATCTATTCATGGCGTGGTGCTCGGCCACAGAACTTGGTCCTGCTAAATGAAGATTTCCCTGC
GCTACGGGTGATTAAGCTGGAACAGAATTACCGTTCTTCGGGGCGTATCTTGAAGGCGGCGAATATTTTGATTGCCAACA
ACCCACATGTTTTTGAAAAGAAACTTTTTTCTGAACTGAGCTATGGCGATGAGTTGAAAGTGATTACCGCCAATAACGAA
GATCATGAAGCGGAGCGGGTGGTCGGCGAGCTTATTGCACATCACTTTGTTAAAAAGACCCAATATAGCGATTACGCCAT
TTTGTATCGTGGGAATCACCAATCGCGGTTGTTTGAAAAATTGCTGATGCAAAACCGTATTCCTTATCGAATTTCGGGTG
GCGATTCATTTTTCTCTCGTCCAGAGATTAAGGATTTGTTGGCTTACCTACGGGTATTGACCAATCAGGATGATGACAGT
GCATTCTTACGAATTGTGAATACACCAAAGCGTGAGATTGGTGCGGCCACCGTCCAAAAATTGGGTGAATGGGCTAATTT
ACGCAATAAGAGCCTGTTTCGGGCCAGTTTTGATTTAGGGTTAGGCGAGCACCTGAAGGGCCGTGGCCTGGAGTCATTGC
AACGTTTTACTCACTGGATGGATGGGATTATTCGTTTGGTTGAGCGTGAACCCGTGGCAGCAGTCCGGGATTTGATCCAC
GGTATTGATTACGAAAGTTGGTTATTCGAAACCGCGCCCAGCCCAAAAGCCGCTGAAATGCGTATGAAAAACGTTAATTT
ATTATTCAGTTGGATGACCGAAATGCTGGAAGGGTCGGAACTGGATGAACCGATGACGCTGACTCAGGTGGTAACGCGTT
TTACCCTGCGTGACATGATGGAGCGAGGGGAGAGTGATGAAGAACTGGATCAAGTGCAATTAATGACGTTGCATGCCTCT
AAAGGGCTGGAGTTCCCTTATGTATTCTTAGTGGGGATGGAAGAGGGGTTGCTGCCGCATCAAAGCAGCATTGATGAAGA
TAATGTGGATGAAGAACGGCGGTTGGCTTATGTGGGGATCACCCGCGCCCAGCGTGAACTGTTCTTCACCCTGTGTAAAG
AACGGCGGCAATACGGCGAGTTGATTCGGCCAGAACCTAGCCGTTTTCTGATGGAGTTGCCGCAGGACGATTTAAACTGG
GAAAACGAACGTAAAGCCGTCAGCCCGGAAGAGCGTATGCAAAAAGGCCAGAGTCATCTGGCCAATCTTCGTGCTCAGTT
AGCGAATGCGAAGAAACCGTAG

Upstream 100 bases:

>100_bases
TGCGTGCGTATTGCCAATCAAAAAAGCAGGCAGTTTTTTTTCATCTGTGGCTTTCTGGTACAATTCGCTTCCCCGTAAAC
CCATTGAGCAACCGAGCGTC

Downstream 100 bases:

>100_bases
TTAATATGAAAAGCCGTAGTTAACACGAAAAGCAGCAGTTACAGTAGTGAAGATAATAACGGGCAGTCTATTGCCCGTTA
GCCATGCATTAAGGCGCGAC

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MRLNPSQQEAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRQCGYQPKHIAAVTFTNKAAREMKERVAQTLGRKEARGLM
IATFHTLGLEIIKKEYVALGMKSNFSLFDAQDQMGLLKDLTHKWLEDDKTLLQQLVSAISNWKNDLLDPAAAAATARSER
DKLFVHCYGLYDAHLKACNVLDFDDLISLPTLLLQKNLEVRERWQNRLRYLLVDEYQDTNTSQYQMVKLLVGNRARFTVV
GDDDQSIYSWRGARPQNLVLLNEDFPALRVIKLEQNYRSSGRILKAANILIANNPHVFEKKLFSELSYGDELKVITANNE
DHEAERVVGELIAHHFVKKTQYSDYAILYRGNHQSRLFEKLLMQNRIPYRISGGDSFFSRPEIKDLLAYLRVLTNQDDDS
AFLRIVNTPKREIGAATVQKLGEWANLRNKSLFRASFDLGLGEHLKGRGLESLQRFTHWMDGIIRLVEREPVAAVRDLIH
GIDYESWLFETAPSPKAAEMRMKNVNLLFSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESDEELDQVQLMTLHAS
KGLEFPYVFLVGMEEGLLPHQSSIDEDNVDEERRLAYVGITRAQRELFFTLCKERRQYGELIRPEPSRFLMELPQDDLNW
ENERKAVSPEERMQKGQSHLANLRAQLANAKKP

Sequences:

>Translated_673_residues
MRLNPSQQEAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRQCGYQPKHIAAVTFTNKAAREMKERVAQTLGRKEARGLM
IATFHTLGLEIIKKEYVALGMKSNFSLFDAQDQMGLLKDLTHKWLEDDKTLLQQLVSAISNWKNDLLDPAAAAATARSER
DKLFVHCYGLYDAHLKACNVLDFDDLISLPTLLLQKNLEVRERWQNRLRYLLVDEYQDTNTSQYQMVKLLVGNRARFTVV
GDDDQSIYSWRGARPQNLVLLNEDFPALRVIKLEQNYRSSGRILKAANILIANNPHVFEKKLFSELSYGDELKVITANNE
DHEAERVVGELIAHHFVKKTQYSDYAILYRGNHQSRLFEKLLMQNRIPYRISGGDSFFSRPEIKDLLAYLRVLTNQDDDS
AFLRIVNTPKREIGAATVQKLGEWANLRNKSLFRASFDLGLGEHLKGRGLESLQRFTHWMDGIIRLVEREPVAAVRDLIH
GIDYESWLFETAPSPKAAEMRMKNVNLLFSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESDEELDQVQLMTLHAS
KGLEFPYVFLVGMEEGLLPHQSSIDEDNVDEERRLAYVGITRAQRELFFTLCKERRQYGELIRPEPSRFLMELPQDDLNW
ENERKAVSPEERMQKGQSHLANLRAQLANAKKP
>Mature_673_residues
MRLNPSQQEAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRQCGYQPKHIAAVTFTNKAAREMKERVAQTLGRKEARGLM
IATFHTLGLEIIKKEYVALGMKSNFSLFDAQDQMGLLKDLTHKWLEDDKTLLQQLVSAISNWKNDLLDPAAAAATARSER
DKLFVHCYGLYDAHLKACNVLDFDDLISLPTLLLQKNLEVRERWQNRLRYLLVDEYQDTNTSQYQMVKLLVGNRARFTVV
GDDDQSIYSWRGARPQNLVLLNEDFPALRVIKLEQNYRSSGRILKAANILIANNPHVFEKKLFSELSYGDELKVITANNE
DHEAERVVGELIAHHFVKKTQYSDYAILYRGNHQSRLFEKLLMQNRIPYRISGGDSFFSRPEIKDLLAYLRVLTNQDDDS
AFLRIVNTPKREIGAATVQKLGEWANLRNKSLFRASFDLGLGEHLKGRGLESLQRFTHWMDGIIRLVEREPVAAVRDLIH
GIDYESWLFETAPSPKAAEMRMKNVNLLFSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESDEELDQVQLMTLHAS
KGLEFPYVFLVGMEEGLLPHQSSIDEDNVDEERRLAYVGITRAQRELFFTLCKERRQYGELIRPEPSRFLMELPQDDLNW
ENERKAVSPEERMQKGQSHLANLRAQLANAKKP

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=668, Percent_Identity=85.0299401197605, Blast_Score=1189, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=648, Percent_Identity=38.4259259259259, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1787196, Length=359, Percent_Identity=29.2479108635098, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6322369, Length=734, Percent_Identity=27.1117166212534, Blast_Score=197, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6324477, Length=696, Percent_Identity=21.9827586206897, Blast_Score=100, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77318; Mature: 77318

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLNPSQQEAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRQCGYQPKHIAAVTFTNKAA
CCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCHHH
REMKERVAQTLGRKEARGLMIATFHTLGLEIIKKEYVALGMKSNFSLFDAQDQMGLLKDL
HHHHHHHHHHHCHHHHCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHH
THKWLEDDKTLLQQLVSAISNWKNDLLDPAAAAATARSERDKLFVHCYGLYDAHLKACNV
HHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEHHHHHHHHCCC
LDFDDLISLPTLLLQKNLEVRERWQNRLRYLLVDEYQDTNTSQYQMVKLLVGNRARFTVV
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCEEEEEE
GDDDQSIYSWRGARPQNLVLLNEDFPALRVIKLEQNYRSSGRILKAANILIANNPHVFEK
CCCCCHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCEEEEEEEEEECCCCHHHH
KLFSELSYGDELKVITANNEDHEAERVVGELIAHHFVKKTQYSDYAILYRGNHQSRLFEK
HHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHH
LLMQNRIPYRISGGDSFFSRPEIKDLLAYLRVLTNQDDDSAFLRIVNTPKREIGAATVQK
HHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHH
LGEWANLRNKSLFRASFDLGLGEHLKGRGLESLQRFTHWMDGIIRLVEREPVAAVRDLIH
HHHHHHCCCCHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
GIDYESWLFETAPSPKAAEMRMKNVNLLFSWMTEMLEGSELDEPMTLTQVVTRFTLRDMM
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ERGESDEELDQVQLMTLHASKGLEFPYVFLVGMEEGLLPHQSSIDEDNVDEERRLAYVGI
HCCCCHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHEEEEEH
TRAQRELFFTLCKERRQYGELIRPEPSRFLMELPQDDLNWENERKAVSPEERMQKGQSHL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ANLRAQLANAKKP
HHHHHHHHCCCCC
>Mature Secondary Structure
MRLNPSQQEAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRQCGYQPKHIAAVTFTNKAA
CCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCHHH
REMKERVAQTLGRKEARGLMIATFHTLGLEIIKKEYVALGMKSNFSLFDAQDQMGLLKDL
HHHHHHHHHHHCHHHHCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHH
THKWLEDDKTLLQQLVSAISNWKNDLLDPAAAAATARSERDKLFVHCYGLYDAHLKACNV
HHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEHHHHHHHHCCC
LDFDDLISLPTLLLQKNLEVRERWQNRLRYLLVDEYQDTNTSQYQMVKLLVGNRARFTVV
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCEEEEEE
GDDDQSIYSWRGARPQNLVLLNEDFPALRVIKLEQNYRSSGRILKAANILIANNPHVFEK
CCCCCHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCEEEEEEEEEECCCCHHHH
KLFSELSYGDELKVITANNEDHEAERVVGELIAHHFVKKTQYSDYAILYRGNHQSRLFEK
HHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHH
LLMQNRIPYRISGGDSFFSRPEIKDLLAYLRVLTNQDDDSAFLRIVNTPKREIGAATVQK
HHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHH
LGEWANLRNKSLFRASFDLGLGEHLKGRGLESLQRFTHWMDGIIRLVEREPVAAVRDLIH
HHHHHHCCCCHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
GIDYESWLFETAPSPKAAEMRMKNVNLLFSWMTEMLEGSELDEPMTLTQVVTRFTLRDMM
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ERGESDEELDQVQLMTLHASKGLEFPYVFLVGMEEGLLPHQSSIDEDNVDEERRLAYVGI
HCCCCHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHEEEEEH
TRAQRELFFTLCKERRQYGELIRPEPSRFLMELPQDDLNWENERKAVSPEERMQKGQSHL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ANLRAQLANAKKP
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]