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The map label for this gene is folK [H]

Identifier: 170025686

GI number: 170025686

Start: 3813579

End: 3814058

Strand: Direct

Name: folK [H]

Synonym: YPK_3471

Alternate gene names: 170025686

Gene position: 3813579-3814058 (Clockwise)

Preceding gene: 170025685

Following gene: 170025687

Centisome position: 81.32

GC content: 53.54

Gene sequence:

>480_bases
ATGATCCGGGTCTATATCGCGCTGGGTAGCAATTTGGCCATGCCACTACAACAGGTTAGCGCGGCACGGGAAGCCTTGGC
GCATCTGCCGCGTAGCCGGTTAGTCGCGTGTTCGCCACTTTACCGCACCAAGCCATTAGGCCCACAGGATCAACCCGATT
TTCTCAATGCCGTGGTCGCACTGGATACGTCGTTGCCACCAGAGCAGTTGCTGGATCACACACAAGCGATCGAACGCAAC
CAAGGCCGGGTGAGAAAAGAGCAGCGCTGGGGGCCACGGACGCTGGATCTGGATATTATGTTGTATGGCGATCAGGTGAT
AAAAACTGATCGCCTAACGATTCCGCATTATGGTCTGAAAGCGCGTGAATTTATGCTCTATCCACTGGCAGATATCGCCC
CAGATCTTATTTTCCCCGATGGCGAATCCCTATCAGAATGCCTTAAGCGGGTCGATAAGAACGGCTTGGTTCTCTGGTAA

Upstream 100 bases:

>100_bases
TCCATGCTGAATACATTAGGTGCTGATCCTGCGCCACGGCGTTCACGTCCACGCCGCCCACGCAAGCCAGTACCCCGTAA
AGAAGGGGTATAATTTTACC

Downstream 100 bases:

>100_bases
TATCGCCGGCTCACACATGGCAAGATCCTCTGTTTCCCCCGTTCTTTTTATTCTTCGATAGCCAGACTCTGCCCGTGCGT
TGAGCCACTCATTACGTTAT

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MIRVYIALGSNLAMPLQQVSAAREALAHLPRSRLVACSPLYRTKPLGPQDQPDFLNAVVALDTSLPPEQLLDHTQAIERN
QGRVRKEQRWGPRTLDLDIMLYGDQVIKTDRLTIPHYGLKAREFMLYPLADIAPDLIFPDGESLSECLKRVDKNGLVLW

Sequences:

>Translated_159_residues
MIRVYIALGSNLAMPLQQVSAAREALAHLPRSRLVACSPLYRTKPLGPQDQPDFLNAVVALDTSLPPEQLLDHTQAIERN
QGRVRKEQRWGPRTLDLDIMLYGDQVIKTDRLTIPHYGLKAREFMLYPLADIAPDLIFPDGESLSECLKRVDKNGLVLW
>Mature_159_residues
MIRVYIALGSNLAMPLQQVSAAREALAHLPRSRLVACSPLYRTKPLGPQDQPDFLNAVVALDTSLPPEQLLDHTQAIERN
QGRVRKEQRWGPRTLDLDIMLYGDQVIKTDRLTIPHYGLKAREFMLYPLADIAPDLIFPDGESLSECLKRVDKNGLVLW

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=159, Percent_Identity=62.2641509433962, Blast_Score=208, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6324073, Length=156, Percent_Identity=30.1282051282051, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 17970; Mature: 17970

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVYIALGSNLAMPLQQVSAAREALAHLPRSRLVACSPLYRTKPLGPQDQPDFLNAVVA
CEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHEEECCCCHHCCCCCCCCCHHHHHHHHH
LDTSLPPEQLLDHTQAIERNQGRVRKEQRWGPRTLDLDIMLYGDQVIKTDRLTIPHYGLK
HCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEEEEEEECCEEEECCCEECCCCCCC
AREFMLYPLADIAPDLIFPDGESLSECLKRVDKNGLVLW
CHHEEEECHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEC
>Mature Secondary Structure
MIRVYIALGSNLAMPLQQVSAAREALAHLPRSRLVACSPLYRTKPLGPQDQPDFLNAVVA
CEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHEEECCCCHHCCCCCCCCCHHHHHHHHH
LDTSLPPEQLLDHTQAIERNQGRVRKEQRWGPRTLDLDIMLYGDQVIKTDRLTIPHYGLK
HCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEEEEEEECCEEEECCCEECCCCCCC
AREFMLYPLADIAPDLIFPDGESLSECLKRVDKNGLVLW
CHHEEEECHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1325970; 8202364; 9278503; 1657875; 2537812; 10378268; 10452528 [H]